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Two new parameters of the third chromosome mutator gene, mu, in Drosophila melanogaster are described. The mutator significantly alters both meiotic chromosome disjunction and crossing over in females, but not in males. In meiotic phenotype, the mutator resembles several recently described “meiotic” mutants of D. melanogaster. However, previous studies have demonstrated that the mutator also functions premeiotically. The mutator appears to be a unique mutant which functions exclusively in females during both mitotic and meiotic cell divisions.

ummary Just as group A1 cells exposed to formol-saline are first weakened and then lose their agglutinability to anti-A sera, D positive formoltreated red cells show an essentially similar change. The resistance of the D antigen to formol-treatment is considerably less, however, than that of the A1 antigen, suggesting a possible chemical difference between the antigens of the Rh and ABO systems. The resistance of the red cells varies with their antigenic strength and may therefore be used for the quantitative assessment of the D antigen content. Resume Au meme titre que les erythrocytes A1 dont l'agglutinabilite par les serums anti-A diminue tout d'abord pour disparaitre ensuite lorsqu'ils sont en solution saline formolee, les erythrocytes D positifs, dans le meme milieu, presentent en principe une alteration similaire. Toutefois, la resistance de l'antigene D au traitement formole est beaucoup moins grande que celle de l'antigene A, ce qui laisse supposer une eventuelle difference dans la composition chimique des antigenes du systeme Rhesus et des antigenes du systeme ABO. La resistance des erythrocytes au traitement formole varie avec la quantite d'antigene et, de ce fait, peut ere utilisee pour determiner quantitativement la contenance en antigene D. Zusammenfassung D-positive Erythrozyten werden in derselben Weise wie A1-Erythrozyten durch Formalin-Kochsalzlosung beeinflust: Ihre Agglutinabilitat durch Anti-D-Seren wird zunachst abgeschwacht, spater vollig aufgehoben. Das D-Antigen ist gegenuber der Formalinbehandlung wesentlich weniger resistent als das A1-Antigen. Das unterschiedliche Verhalten der beiden Antigene beruht hochstwahrscheinlich auf chemischen Strukturunterschieden. Die Resistenz gegenuber der Formalinbehandlung ist abhangig vom Gehalt der betreffenden Erythrozyten an D-Antigen. Der Formalintest erlaubt somit eine quantitative Bestimmung des D-Antigengehaltes der Erythrozyten.

Summary Extracts of the albumin glands of Helix aspersa contain in addition to the anti-A-like haemagglutinin also a haemolysin. It was possible by chromatographic resolution to separate the haemolysin from the haemagglutinin. The haemolysin is unrelated to the ABO system as it reacts also with Oh (Bombay) red cells. Haemolysins have been found in crude extracts of various seeds, mushrooms and even molluscs. However all these haemolysins do not need the presence of complement for haemolysis. It seems that the haemolysin reported in this paper is the first which needs probably complement for haemolytic activity.

Summary. The presence of B substance in saline extracts of albumen glands of Helix pomatia has been reported by Kimet al. [5] and the agglutination of Hela cells by anti-AHel mentioned by Uhlenbbucket al. [10]. We report in this paper serological differences between related species of snails revealed by haemolysis, agglutination of Hela cells and presence of B-like substance. The same snail substances have been used as in Part I of this report. Saline extracts of seeds were prepared as described in [2] and anti-BST was the same as that used for experiments in Part I of this report.

The solution is obtained to the problem of the steady one-dimensional flow of an incompressible, viscous, electrically conducting fluid through a circular pipe in the presence of an applied (transverse) uniform magnetic field. A no-slip condition on the velocity is assumed at the non-conducting wall. The solution is exact and thus valid for all values of the Hartmann number. Excellent agreement exists between the present theoretical results and the experimental values obtained by Hartmann & Lazarus (1937) in the low to medium Hartmann number range. The high Hartmann number case is treated by Shercliff (1962) in the following paper.

Summary An antigen absorption test using anti-A serum and A substance is described. Using a build-up technique with the antigen absorption test, visual agglutination of formerly inagglutinable A cells, including a mixed-field appearance, has been obtained by both anti-A sera of human origin and Dolichos biflorus extract. Resume Les auteurs decrivent un test d'absorption d'antigenes dans lequel ils ont utilise un serum anti-A et de la substance A. Pour pratiquer ce test d'absorption, ils ont mis au point une technique basee sur le principe des reactions en chaine grâce a laquelle ils ont pu obtenir avec des serums anti-A d'origine humaine et un extrait de dolichos biflorus des agglutinations, presentant souvent l'image de l'agglutination mixte, d'erythrocytes A initialement inagglutinables. Zusammenfassung Es wird ein Antigen-Absorptionstest mit Anti-A-Serum und A-Gruppensubstanz beschrieben. Dieser Test wurde verwendet, um mit einer Mehrphasentechnik nicht agglutinierbare A-Zellen zu agglutinieren. Dies gelang sowohl mit menschlichen Anti-A-Seren als auch mit Dolichos-biflorus-Extrakten, wobei Mischzellbilder beobachtet wurden.

A general analysis of plane wave propagation across thin stratified plasma sheaths (for example, the boundary layer on slender entry vehicles) is presented, including arbitrary polarization, incidence, and plasma properties. Accurate slide-rule calculations of transmission and reflection can be made readily from the approximate expressions for a wide range of applications. Parametric results are presented graphically, demonstrating the salient effects and tradeoffs and providing a convenient reference for preliminary design analyses. Of particular note is the evaluation of the increase in transmitted signal strength for TM waves (where the electric field is polarized in the plane of incidence) as the incidence varies from side-on to grazing.

Antiglobulin Fi. reacts with its own IgG, with some human IgG's of all four subclasses and with rabbit antibody IgG. The determinant is present in the sera of all races tested. It has also been found in the sera of apes and Cercopithecus monkeys, but only in a few high-titer anti-D IgG. It is present in cord serum and serum of aged subjects but is present in very small amounts, if at all, in early fetal sera. It is tentatively assumed that it is present in a basic amino acid sequence of the Fc fragment of the heavy chain of IgG.

Es wird uber ungewohnliche Blutgruppenbefunde bei Leukamie berichtet. Es handelt sich um eine Schwachung bis zu vollkommenem Schwund der Antigene. Diese Anderung wird mit den bei Gesunden gefundenen Varianten und den durch chemische Substanzen erzeugten Veranderungen verglichen. Die Moglichkeit einer somatischen Mutation von «Retikulumzellen» als Ursache der beschriebenen Antigen-veranderungen wird diskutiert.

Summary The passive hemagglutination procedure using a chromium chloride solution to bind antigens and antibodies to red cells has been investigated. The CrCl3 method of coating red cells is rapid and simple, and serologic sensitivity and immunologic specificity are retained. The present studies showed that group O human red cells coated with antigen by the CrCl3 procedure can be used in a number of serologic reactions. In particular, use of CrCl3-treated cells as immunoadsorbent provides a rapid method for the preparation of monospecific antisera to human immunoglobulins. The immunoglobulin content of pathologic sera can be estimated by the degree to which the pathologic serum inhibits an appropriate agglutination system consisting of cells coated with γG-, γA- or γM-globulin by the CrCl3 method and of homologous antibody. “Purified” immunoglobulin preparations can be assessed for contamination by similar inhibition of agglutination of red cells coated with immunoglobulins by the CrCl3 method.

The infrared and laser–Raman spectra of liquid dimethyliodoarsine, (CH3)2AsI, have been recorded. Assignments of the observed frequencies have been made to the 24 normal modes of vibration of the molecule. A normal coordinate calculation has been carried out in support of the assignment.

Human antibodies to IgA globulin have been demonstrated in human sera by a passive hemagglutination method using as antigens IgA myeloma proteins coupled to inert indicator red cells. The vast majority of sera containing such agglutinators occur in patients with little or no serum IgA and normal levels of IgG and/or IgM. Specificity of the antibodies was shown by inhibition of agglutination. The antibodies of different sera appear directed toward different antigenic sites in the alpha chains. Such sera may prove useful in demonstrating allotypic genetically determined antigens in IgA molecules analogous to the Gm factors of IgG molecules.

A questionnaire containing 62 items relating to the New Left and 28 items measuring anomia, authoritarianism, and Machiavellianism was given to 153 entering freshmen at Columbia in the fall of 1968. The five factors emerging from a factor analysis of responses to the items were labeled: New Left Philosophy, Revolutionary Tactics, Machiavellian Tactics, Machiavellian Cynicism, and Traditional Moralism. Students who had been politically active prior to coming to college scored higher than the rest of the group on New Left Philosophy, Revolutionary Tactics, and Machiavellian Cynicism, but scored lower on Machiavellian‘Tactics and Traditional Moralism. In the spring of 1969, a revised set of items was given to students in introductory psychology classes at Columbia University and New York University. While the findings from these subjects replicated those found with the Columbia freshmen, they showed subtle differences which led to speculations about the radicalization process. Also discussed are the relationships between the nature of political participation and all of the factors, with special emphasis on the discrepancy between Machiavellian Tactics and Cynicism.

(1971). An Empirical Approach to Issues of Selection and Evaluation in Group Therapy. International Journal of Group Psychotherapy: Vol. 21, No. 4, pp. 456-469.

In this paper an attempt has been made to evaluate a method of motion study pertaining to moving machinery. The test object was a 13-ton capacity punch press and the motion study was performed with a high-speed motion picture camera. It is shown that the motion picture camera produces: (1) a visual indication of the actual occurrence which allows an understanding that nonvisual means could not afford; (2) a method of study which does not influence the system studied in the least; and (3) a record of the occurrence which has very little inherent error.

The results of agglutination and agglutination-inhibition experiments with two spong extracts (Axinella sp. and Cliona celata) and three snail extracts (Achatina granulata, Theba pisana and Helicella uirgata, banded form) are reported. The extracts which show D-galactose specificity and are also inhibited by sugars related to D-galactose or containing it, have been subdivided into 1. a subgroup with no blood group specificity to which belong Axinella sp., Cliona celata and Ricinus communis extract and 2. another subgroup with B-like specificity to which belong extracts of Helicella virgata (banded form), Bandeiraea simplicifolia and Fomes fomentarius extracts. Extracts of subgroup 1. but not of subgroup 2. are inhibited by Pn XIV poly-saccharide. In spite of anti-B-like specificity, extracts belonging to subgroup 2. are just as well or even better inhibited by NAcgal than by D-galactose. The extracts of Achatina granulata and Theba pisana lack D-galactose specificity. Achatina granulata extract was inhibited by NAcgal, NAcglu and NANA; Theba pisana extract only by NANA. Theba pisana extract agglutinated only neuraminidase-treated red cells, Helicella virgata (banded form) extract only enzyme-treated B cells.

Resume Les frequences du facteur Gm(4) ont ete recherchees parmi 1 799 sujets appartenant a diverses populations blanches, noires et jaunes. Certains echantillons ont ete testes egalement vis-a-vis des facteurs Gm(1, 2, 4, 6, 8, 10 et 12). De cette analyse se degage l'extreme heterogeneite de la frequence du Gm(4). Si, parmi les leucodermes, le Gm(4) est presque toujours associe aux Gm(8), Gm(10) et Gm(12), il n'en est pas de meme chez les melandoermes et les xanthodermes ou les frequences de ces differents facteurs sont tres variables.

A detailed analysis of fully developed magnetohydrodynamic flow through rectangular and circular channels is presented for nonzero finite values of the uniform wall conductance for the rectangular channel and for small values of for the circular geometry. These results are combined with existing exact and approximate calculations in a semigraphical procedure to obtain the ratio of mean velocity to pressure gradient as a function of arbitrary values of Hartmann number and k, bk, c^dk — constants of integration, Eqs. (17) and (28) A = cross-sectional area of channel Ak, Bk = constant of integration, Eq. (37) C = cross-sectional contour of interior channel wall E = electric field strength f,g = transformed variables, Eq. (6) F} G = transformed variables, Eq. (32)

A 9-year-old female presented with mitral regurgitation, heart failure, fever, abnormal serum proteins, elevated sedimentation rate, and elevated antihyaluronidase titer. At first thought to have rheumatic fever, she initially responded favorably to medical therapy which included corticosteroids. Evidence of progressive disease led to cardiac catheterization and angiography which demonstrated a left atrial myxoma which was successfully excised. Review of the literature reveals that 23 children with left atrial myxomas have been reported. These may present with embolic, obstructive, or constitutional symptoms. Most children have clinical evidence of mitral regurgitation. Associated heart failure, anemia, elevated sedimentation rate, elevated antihyaluronidase titer and initial favorable response to medical therapy often lead to the incorrect diagnosis of acute or chronic rheumatic heart disease. In these patients, increased endogenous hyaluronic acid excretion from tumor cells may lead to increased hyaluronidase and antihyaluronidase activity, abnormal serum proteins and elevated sedimentation rate.

When large numbers of Ehrlich ascites tumor cells were transplanted into the peritoneal cavity of mice, they were found capable of taking up tritiated thymidine within one minute after transplantation. There were only a few necrotic cells after transfer, and the percentage of cells labeled by tritiated thymidine remained constant up to 30 hr after inoculation. These findings indicated that cell death was negligible and that Ehrlich cells transplanted into new hosts continued to synthesize DNA without an appreciable lag. A temporary depression of the mitotic index and a slight delay in the appearance of labeled mitoses suggested a lengthening of that phase of the cell cycle immediately preceding mitosis. However, the length of the DNA synthesis phase did not differ from the values found in previous experiments with tumors in various stages of growth. The percentage of Ehrlich ascites tumor cells labeled by a single injection of tritiated thymidine decreased after the first few days of tumor growth, but it promptly increased again when these cells from tumors in advanced stages of growth were transplanted into new hosts.

Although both methods are employed with varying success, each suffers from disad­vantages when large numbers of well-spread metaphases are desired from a large sample of adult individuals. For example, tissue squashes· often result in low numbers of poorly spread mitotic preparations and in addition require some degree of manipulatory dexterity. Cell cul­ture techniques, on the other hand, while pro­viding excellent spread numbers and resolution of chromosome detail, require laboratory skills and equipment not always available. Moreover, the cost of cell culture procedures necessary for karyotyping a large sample of adult fish would preclude its use in all but very specialized lab­oratories. At the Davis trout genetics laboratory, the possibility of using karyotypic differences as racial and/or species markers among salmonid fish is being pursued. Since a large number of adult individuals are examined, ~rnate meth­ods of chromosome karyotyping have been sought which are both inexpensive and simple in design and execution. The procedure de­scribed below has satisfied both criteria, and is currently employed as the sole method of chro­mosome karyotyping at the Davis laboratory. MATERIALS AND METIIODS

I a lecent article, Drake has presented a method for determining the minimum length of crossed-field MHD accelerators with specified entrance and exit conditions Drake has assumed that the temperature (which is pioportional to enthalpy for a perfect gas) was constant throughout the accelerator and has developed a numerical procedure for finding the minimum length In many practical cases, the joule heating per unit volume is small compared with the net local electrical power input For these cases, it is possible to solve the minimum-length problem in closed form This solution is indicated herein for the case of constant enthalpy, magnetic field, and electrical conductivity A brief comparison is made with the constant enthalpy accelerator solution presented in Ref 2 The notation and configuration are the same as in Ref 2 The one-dimensional equations of motion, neglecting heat conduction, viscous effects, and ion slip, and assuming constant enthalpy, are as follows

It is proposed that mineral samples from the lunar surface be examined for possible measurement of the long-term lunar cosmic-ray environment. Observation of radiation-induced tracks in suitable lunar specimens could reveal, in principle, the primary cosmic-ray fluence for an epoch comparable with the lunar age. Such data would provide quantitative bounds on the long-term variation of cosmic radiation in the vicinity of the earth and thereby test necessary conditions for the hypothesis of cosmic-ray induced evolutionary effects from supernovae.

10-Second summary Concern for the indignities heaped upon man by an endless number of physical and mental onslaughts is bringing dignity to the rapidly developing field of emergency medicine. A similar concern is urged for the good name of this absorbing and dynamic specialty.

Summary Anti-complement antiglobulin was produced by immunising rabbits with formol-treated A1 cells coated with heat-inactivated anti-A serum. Anti-human-gammaglobulin was produced by immunising rabbits with their own formol-treated cells. The mechanism of production of the two antiglobulin reagents is discussed. Resume De l'anti-globuline anti-complement est produite par immunisation de lapins par des erythrocytes A1 traites au formol et sensibilises par un serum anti-A inactive par la chaleur. De la gammaglobuline anti-humaine est produite par immunisation de lapins avec leurs propres erythrocytes traites au formol. Le mecanisme de production de ces deux anti-globulines est discute. Zusammenfassung Durch Immunisierung von Kaninchen mit formalinbehandelten A1-Zellen, welche mit hitzeinaktiviertem Anti-A-Serum beladen worden waren, gelang es Anti-Komplement-aktive Antiglobulinseren herzustellen. Durch Immunisierung von Kaninchen mit ihren eigenen formalinbehandelten Erythrozyten gelang es, Anti-Human-Gammaglobulinseren zu erzeugen. Der Mechanismus der Entstehung der beiden Antiglobulinreagenzien wird diskutiert.