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Your search keyword '"Prokaryotic initiation factor-1"' showing total 4 results
Start Over Descriptor "Prokaryotic initiation factor-1" Publication Year Range More than 50 years ago
4 results on '"Prokaryotic initiation factor-1"'

1. Myosin synthesis and specificity of eukaryotic initiation factors

Author

Arthur W. Rourke and Stuart M. Heywood

Subjects
Electrophoresis, Peptide Biosynthesis, Erythrocytes, Reticulocytes, Muscle Proteins, Chick Embryo, Myosins, Biochemistry, Hemoglobins, Eukaryotic translation, Eukaryotic initiation factor, Animals, Initiation factor, Magnesium, RNA, Messenger, Amino Acids, Peptide Chain Initiation, Translational, Prokaryotic initiation factor, Carbon Isotopes, Cell-Free System, Chemistry, Prokaryotic initiation factor-2, Muscles, Prokaryotic initiation factor-1, Blood Proteins, EIF4A1, Chromatography, Ion Exchange, Eukaryotic translation initiation factor 4 gamma, Cell biology, Molecular Weight, Chromatography, Gel, Potassium, Rabbits, Carrier Proteins, Chickens, Ribosomes, Peptide Hydrolases
Published
1972
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2. A Complex Between Initiation Factor IF2, Guanosine Triphosphate, and fMet-tRNA f : An Intermediate in Initiation Complex Formation

Author

Umadas Maitra, Prasanta R. Chakraborty, and Arthur H. Lockwood

Subjects
GTP', Uracil Nucleotides, Phenylalanine, Polynucleotides, Cytosine Nucleotides, Guanosine triphosphate, Biology, Tritium, chemistry.chemical_compound, Adenosine Triphosphate, Methionine, Cytosine nucleotide, Bacterial Proteins, RNA, Transfer, Eukaryotic initiation factor, Escherichia coli, Initiation factor, Peptide Chain Initiation, Translational, eIF2, Binding Sites, Multidisciplinary, Prokaryotic initiation factor-2, Prokaryotic initiation factor-1, Phosphorus Isotopes, Adenosine Monophosphate, Guanine Nucleotides, RNA, Bacterial, chemistry, Biochemistry, Chromatography, Gel, Puromycin, Guanosine Triphosphate, Biological Sciences: Biochemistry, Ribosomes, Protein Binding
Abstract

Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNA f . This complex transfers both fMet-tRNA f and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNA f in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNA f ] is an intermediate in the initiation of protein synthesis in Escherichia coli .

Published
1971
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3. Discrimination between eukaryotic and prokaryotic, and formylated and non-formylated, initiator tRNAs by eukaryotic initiation factor EIF-3

Author

Ira G. Wool and Rajinder Singh Ranu

Subjects
Formic Acid Esters, Saccharomyces cerevisiae, macromolecular substances, environment and public health, Mice, Structure-Activity Relationship, Methionine, Eukaryotic translation, RNA, Transfer, Species Specificity, Peptide Initiation Factors, Eukaryotic initiation factor, Escherichia coli, Animals, heterocyclic compounds, Prokaryotic initiation factor, Genetics, Multidisciplinary, Chemistry, Prokaryotic initiation factor-2, Eukaryotic transcription, Prokaryotic initiation factor-1, hemic and immune systems, EIF4A1, Eukaryotic translation initiation factor 4 gamma, Kinetics, RNA, Bacterial, Biochemistry, Guanosine Triphosphate
Abstract

Discrimination between eukaryotic and prokaryotic, and formylated and non-formylated, initiator tRNAs by eukaryotic initiation factor EIF-3

Published
1975
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4. Polypeptide chain initiation in Caulobacter crescentus without initiation factor IF-1

Author

Wlodzimierz Szer and Steven Leffler

Subjects
Formates, genetic processes, Oligonucleotides, Biology, medicine.disease_cause, Biochemistry, Ribosome, Microbiology, Methionine, Drug Stability, RNA, Transfer, Species Specificity, Peptide Initiation Factors, medicine, Escherichia coli, Initiation factor, Carbon Radioisotopes, RNA, Messenger, Peptide Chain Initiation, Translational, Molecular Biology, Bacteria, Oligonucleotide, Caulobacter crescentus, Prokaryotic initiation factor-1, Temperature, Cell Biology, biochemical phenomena, metabolism, and nutrition, Ribosomal RNA, biology.organism_classification, Chromatography, Ion Exchange, Ammonium Sulfate, Chromatography, Gel, bacteria, RNA, Viral, Electrophoresis, Polyacrylamide Gel, biological phenomena, cell phenomena, and immunity, Ribosomes
Abstract

Two subspecies of initiation factor IF-2 have been isolated from ribosomal washes of Caulobacter crescentus and purified to homogeneity. Both factors, C-IF-2a and b, have the same functional properties and are interchangeable with Escherichia coli IF-2 for the formation of initiation complexes with either E. coli or C. crescentus ribosomes. The C. crescentus ribosomal wash contains no detectable IF-1 activity and the formation of initiation complexes with C. crescentus ribosomes has no requirement for added IF-1. With E. coli ribosomes C-IF-2a or b, or the crude C. crescentus ribosomal wash, must be supplemented with E. coli IF-1 for optimum initiation complex formation. IF-1 activity resides in C. crescentus salt-washed 30 S subunits.

Published
1974

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