1. CHANGES IN THE ENZYME ACTIVITY OF SOLUBLE PROTEIN FRACTIONS IN THE COURSE OF FOLIAR SENESCENCE IN PERILLA FRUTESCENS (L.) BRITT
- Author
-
H. W. Woolhouse and C. G. Kannangara
- Subjects
chemistry.chemical_classification ,Senescence ,Perilla frutescens ,biology ,Molecular mass ,Physiology ,Fraction (chemistry) ,Plant Science ,biology.organism_classification ,Perilla ,Enzyme assay ,Enzyme ,chemistry ,Biochemistry ,Sephadex ,biology.protein - Abstract
Summary Separation of the soluble proteins from leaves of Perilla by means of gel filtration on a Sephadex Groo column yielded two protein bands with molecular weights of approximately 400,000 and 23,000, which appear to correspond to the well-known Fraction I and Fraction II proteins respectively. The amount of Fraction I protein in the leaves decreased progressively from the time that the leaves became fully expanded whilst the amount of Fraction II protein decreased only in the later stages of senescence. Aleasurements of the activity of several enzymes in successive 4 ml fractions from the column showed that each occupied a different peak position within the two main protein fractions. Fach of the enzymes assayed showed a different pattern of change in the course of senescence of the leaves. These changes in protein content and enzyme activity are discussed in relation to earlier observations on the physiological functioning of senescing leaves of Perilla.
- Published
- 1968