1. D-glucose: preferential binding to brush borders disrupted with tris(hydroxymethyl)aminomethane
- Author
-
Shih-min L. Wu, Robert G. Faust, and Mary L. Faggard
- Subjects
inorganic chemicals ,Tris ,Phlorizin ,Sodium ,chemistry.chemical_element ,Mannose ,Biological Transport, Active ,Tritium ,Medicinal chemistry ,chemistry.chemical_compound ,Adenosine Triphosphate ,Intestinal mucosa ,Chlorides ,D-Glucose ,Cricetinae ,Animals ,heterocyclic compounds ,Hydroxymethyl ,Magnesium ,Intestinal Mucosa ,Tromethamine ,Carbon Isotopes ,Multidisciplinary ,Temperature ,Glucose ,Jejunum ,Phlorhizin ,chemistry ,Biochemistry ,Potassium ,Adenosine triphosphate - Abstract
The actively transported sugar D-glucose binds to brush borders disrupted with tris(hydroxymethyl)aminomethane in preference to D-mannose and L-glucose, which are not actively transported. This preferential binding of D-glucose is not dependent on either added Na(+) or adenosine triphosphatase activity stimulated by Na(+) with K(+) and Mg(2+), but it is temperature-dependent and is completely inhibited by 0.1 millimolar phlorizin and 1 millimolar mercuric chloride.
- Published
- 1967