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The polarography of several aliphatic polyamine complexes of Pb(II), Zn(II), and Ni(II), and the anodic stripping polarography of polyamine complexes of Zn(II), Cd(II), Pb(II), and Cu(II) were studied. The aliphatic polyamines used were ethylenediamine(en), 1,3-propanediamine(1,3-pn), 1,2-propanediamine(1,2-pn), diethylenetriamine(den), and triethylenetetramine(trien). These lead(II) complexes gave a one-step reversible wave which was diffusion-controlled. The relation between the logarithm of the concentration of polyamine and the halfwave potential of the Pb(II) complex was straight, so that one species of complex ion was found to be predominant in the bulk of solution. The ionic species in the bulk of solution were found to be Pb(en)22+, Pb(1,3-pn)22+, Pb(1,2-pn)22+, Pb(den)22+, and Pb(trien)2+. The stability constants of these complexes were calculated and were found to increase in the order of Pb(1,3-pn)22+

The application of anodic potential-step stripping voltammetry for the determination of metal ion was investigated. From the dependence of stripping current (ia,d) on electrode radius, the rotational speed of the stirring rod in electrodeposition and electrodeposition time for Cd(II) in 1 M KCl, the diffusion-controlled process could be theoretically predicted. The calibration curves of Cd(II), Mn(II), Ni(II), Zn(II), and Sb(III) were found to be linear in the range 0.01–0.1 ppm, and those of Tl(I), Cu(II), Pb(II), Sn(II), Bi(III), and In(III) in the range 0.02–0.2 ppm, by analysis at time t=1.25 sec on current-time curves. The ratio ia,d⁄ia,p increased in the order n=3

滲出液の性質を鑑別することは各種の検査方法を用いても必ずしも容易でない.胸水あるいは腹水に健康人O型赤血球を浮遊沈降させると,結核性液では赤血球が大きな集塊を作つて速やかに沈降するが,癌性液その他の原因による貯留液では集塊を作ることなく緩やかに沈降するという事実に基づき,赤血球の凝集と赤血球表面荷電は密接に関係すると思われるので,表面荷電を直接反映する赤血球電気泳動速度を測定した.結核性胸水では赤血球易動度が低下し,癌性胸水では赤血球易動度が上昇し,結核性胸水と癌性胸水は泳動速度に相反する影響を及ぼすことを知つた.また,癌血清は赤血球泳動速度を著明に低下せしめ,なかんずく,肺癌患者血清ではばらつきが少ないことが認められた.

第二水銀イオンの溶出波を用いるく形波ポーラログラフィーによるイオウイオンの定量法について検討した.イオウイオンの波が,支持電解質として用いる市販特級の水酸化ナトリウム中に不純物として含まれているPb2+の波と重なることがわかった.この両者の波は,0.09Mエチレンジアミンテトラ酢酸四ナトリウム塩を添加することにより分離できた.1M水酸化ナトリウム,0.09Mエチレンジアミンテトラ酢酸四ナトリウム塩の支持電解質中で,頂点電位が-0.610V vs. Hg plooloをもつ頂点電流を用いて,イオウイオンの濃度1×10-6M~1×10-5M,1×10-5M~1×10-4Mで,検量線を求めることができた.SO42-,SO32-,S2O32-,SCN-,I-,Br-,Cl-などの陰イオンがイオウイオンに対して100倍以上も含まれていても妨害しない.くりかえしの誤差はおよそ±3%であった.

We take some technical considerations in mikroelectrophoresis for cells. Details of construction of a mikroelectrophoretic apparatus, suitable for study of living cells, are given. The problem of measuring mobility is discussed. Electrophoretic mobility of the red blood cells of healthy persons in various buffer solution are presented.

The polarography of several aliphatic polyamine complexes of Pb(II), Zn(II), and Ni(II), and the anodic stripping polarography of polyamine complexes of Zn(II), Cd(II), Pb(II), and Cu(II) were studied. The aliphatic polyamines used were ethylenediamine(en), 1,3-propanediamine(1,3-pn), 1,2-propanediamine(1,2-pn), diethylenetriamine(den), and triethylenetetramine(trien). These lead(II) complexes gave a one-step reversible wave which was diffusion-controlled. The relation between the logarithm of the concentration of polyamine and the halfwave potential of the Pb(II) complex was straight, so that one species of complex ion was found to be predominant in the bulk of solution. The ionic species in the bulk of solution were found to be Pb(en)22+, Pb(1,3-pn)22+, Pb(1,2-pn)22+, Pb(den)22+, and Pb(trien)2+. The stability constants of these complexes were calculated and were found to increase in the order of Pb(1,3-pn)22+

The application of anodic potential-step stripping voltammetry for the determination of metal ion was investigated. From the dependence of stripping current (ia,d) on electrode radius, the rotational speed of the stirring rod in electrodeposition and electrodeposition time for Cd(II) in 1 M KCl, the diffusion-controlled process could be theoretically predicted. The calibration curves of Cd(II), Mn(II), Ni(II), Zn(II), and Sb(III) were found to be linear in the range 0.01–0.1 ppm, and those of Tl(I), Cu(II), Pb(II), Sn(II), Bi(III), and In(III) in the range 0.02–0.2 ppm, by analysis at time t=1.25 sec on current-time curves. The ratio ia,d⁄ia,p increased in the order n=3