1. Automatic digital data acquisition and computer calculation in amino acid analysis
- Author
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Micah I. Krichevsky, Michael G. Mage, and Jonathan Schwartz
- Subjects
Channel (digital image) ,Fortran ,Computer science ,Noise (signal processing) ,Computers ,Computation ,Digital data ,Biophysics ,Cell Biology ,Photometer ,Biochemistry ,Chemistry Techniques, Analytical ,law.invention ,Data acquisition ,law ,Voltmeter ,Computer graphics (images) ,Amino Acids ,Molecular Biology ,computer ,computer.programming_language - Abstract
Amino acid analysis data acquisition and computation have been automated by (1) measuring the ninhydrin color with a spectrophotometer which is linear with respect to optical density, (2) digitizing the data directly from the photometer circuit by means of a digital voltmeter, and (3) formating and punching the digital data in paper tape so that each data word is identified as to channel of information (i.e., cuvet position and wavelength), and processing the punched data on a computer using a FORTRAN program. The program edits the data with regard to noise, compensates for baseline changes of various types, splits unresolved peaks, and calculates peak areas over a range of optical densities from 0 to 3.000. This system, in daily use in our laboratory, provides increased accuracy and saves approximately 2 man hours per analysis.
- Published
- 1965