1. Quantitative measurement of precipitating antibodies in streptococcal grouping antisera by the single radial immunodiffusion technique
- Author
-
J. Richard George and W K Harrell
- Subjects
Serotype ,Immunodiffusion ,Carbohydrates ,Biology ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Antigen ,medicine ,Methods ,Animals ,General Pharmacology, Toxicology and Pharmaceutics ,Serotyping ,Antiserum ,Radial immunodiffusion ,Antigens, Bacterial ,General Immunology and Microbiology ,Streptococcus ,Immune Sera ,General Medicine ,Precipitin ,Molecular biology ,Precipitin Tests ,Bacterial vaccine ,Agar ,Precipitins ,Evaluation Studies as Topic ,Bacterial Vaccines ,Rabbits ,Clinical Microbiology, Virology, and Immunology - Abstract
A comparative study was made of the single radial immunodiffusion test and the classical quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcal groups A and C antisera. The potency of 21 group A and 54 group C antisera was determined by both methods; purified group-specific carbohydrates were used as antigens. The coefficient of correlation between the results from the two methods was 0.976 for group A antisera and 0.946 for group C antisera. When the concentration of antigen, the volume of antiserum used, and the depth of the antigen-agar mixture are kept constant, the diameter of the precipitin disc is directly related to the concentration of precipitable antibodies present in the antiserum. The use of the radial immunodiffusion test for evaluating and standardizing streptococcal grouping antisera is discussed as well as the advantages and disadvantages of using a concentrated vaccine for producing these antisera.
- Published
- 1972