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An antiserum was produced against the plasma of rats with adrenal regeneration hypertension. When the antiserum was adsorbed with normal rat plasma a single precipitin arc in the pre-albumin zone remained in the antiserum, indicating a specific antibody against the hypertensive factors responsible for this type of hypertension in rats. Following a single injection of the adsorbed antiserum into rats with adrenal regeneration hypertension, the level of blood pressure dropped noticeably. Histologically, the zona glomerulosa of hypertensive rats was hyperplastic, whereas that of rats receiving the antiserum showed marked atrophy.

The fate of human albumin introduced into the skin of rats and the local immunologic responses were studied using fluorescein-antialbumin and rhodamine-albumin conjugates. Alum-precipitated human albumin injected into subcutaneous tissues of 30 rats was detected locally in the extra-cellular space and in the macrophages until the 16th day. Albumin was observed in the macrophages (cytoplasm and in some nuclei) from the fifth post-injection day. Plasma-cell aggregates appeared locally around blood vessels and, in addition, with macrophages and lymphocytes they formed structures reminiscent of lymph follicles. The term “immunofollicle” is suggested for the latter structures. It is reasonable to postulate that antibodies to the introduced antigen (albumin) may be produced in these immunofollicles-the site of antigen injection.