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We have used the technique of human haemopoietic cell culture in agar to study the peripheral blood and bone marrow colony forming capacity of 23 patients with Ph1+ ve chronic granulocytic leukaemia (CGL) before and after treatment. In comparison with normal controls the number of colony forming cells (CFC) is moderately increased (about three-fold) in the bone marrow and enormously increased in the peripheral blood of untreated patients. In the peripheral blood their number in general is related to the total leucocyte count. In patients whose blood counts have been restored to normal by the use of cytotoxic drugs the number of CFC in the peripheral blood is very greatly reduced. In the marrow of treated patients CFC are present in approximately normal numbers. When used as feeder layer sto support the culture of normal bone marrow cells, the peripheral blood leucocytes of untreated patients are a uniformly poor source of colony stimulating factor (CSF) and fractionation experiments suggest that this is not due merely to a relative scarcity of monocytes. After treatment the peripheral blood has normal CSF activity and this is associated with the monocytic cell component. The last data may be explained in either of two ways: it is possible that restoration of the blood of patients with CGL to normal values removes a homeostatic factor suppressing the formation of CSF by functionally normal monocytes, or alternatively treatment with cytotoxic drugs leads to the replacement of defective monocytes by a population of relatively normal CSF producing cells.

The intrinsic form of bronchial asthma remains an enigma. For some it signifies asthma of unknown cause, preferably termed "idiopathic"; to others it is largely a psychosomatic problem, but for many it is still almost wholly equated with "bacterial" or "infective" asthma. At the heart of the last concept lies the question of the relationship of sinus infection to bronchial asthma, which persists as a matter of controversy. This report is offered in the hope of clarifying the issues involved in this problem.Four years ago a clinical research unit, including an allergist and a bacteriologist, was organized in the department of otolaryngology at the Mount Sinai Hospital, to investigate the subject of focal infection 1 as it concerns the role of nasal and sinus disease. This group met one morning a week for three years. Bronchial asthma constituted the largest group of cases studied, and these comprise the case

Some properties of insoluble corneal collagen were studied using the specific depolymerization action of pronase, pepsin, bacterial α-amylase, 6 M urea, 2% semicarbazide, 2% semicarbazide in combination with 0,5 M Na2CO3, and 0,5 M Na2CO3 alone. Pronase and pepsin released 1.9 % and 1.2 % of the telopeptides in bovine corneal collagen similar to the amount released from other connective tissues (skin 2.6% and 1.9%). Pronase, pepsin and α-amylase solubilized 8.8 %, 2.1%, 24.7 % of insoluble bovine corneal collagen, respectively. It can be concluded that cross-links in corneal collagen are located in the body of the collagen molecule and not in the telopeptide region. The results of the polyacrylamide gel electrophoresis indicate that the α2 chain is presumably much more involved in the cross-linking interactions than α1 The only way to solubilize bovine corneal collagen is the simultaneous treatment of the collagen with semicarbazide and Na2CO3, at pH 10, which seems to be a useful tool for further structural studies of collagen in this tissue.

The precision of in situoptical quantitative thin-layer chromatographic analysis is dependent on the following: load application, reversible chromatography, optical-quality thin-layer chromatographic plates, quantitative relation between photometry and concentration, and accurate recognition of location and extent of the chromatographed zone.