1. Terpenoid biosynthesis by chloroplasts isolated in organic solvents
- Author
-
George Britton, Michael J. Buggy, and Trevor W. Goodwin
- Subjects
chemistry.chemical_classification ,Geranylgeranyl pyrophosphate ,Plant Science ,General Medicine ,Horticulture ,Biochemistry ,Terpenoid ,Chloroplast ,Phytol ,chemistry.chemical_compound ,Phytoene ,chemistry ,Geranylgeraniol ,Biosynthesis ,Organic chemistry ,Molecular Biology ,Carotenoid - Abstract
Chloroplastsisolated in organic solvents (“non-aqueous chloroplasts”)incorporate [2-14C]-mevalonate into geranylgeraniol and phytoene. No incorporation into phytol or into other carotenoids was achieved, even in mixtures of non-aqueous chloroplasts with chloroplasts prepared in aqueous media. The incorporation of geranylgeranyl pyrophosphate into phytoene by the non-aqueous chloroplast preparation has been demonstrated, and the stereochemistry of hydrogen loss in phytoene synthesis by non-aqueous chloroplasts has been determined.
- Published
- 1974