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35 results on '"Georg W. Kreutzberg"'

1. Histochemische Untersuchungen oxydativer Enzyme am regenerierenden Nervus ischiadicus der Ratte

Author

Wolfgang Wechsler and Georg W. Kreutzberg

Subjects
biology, Regeneration (biology), Succinate dehydrogenase, Malate dehydrogenase, Pathology and Forensic Medicine, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Biochemistry, chemistry, Lipoamide Dehydrogenase, Lactate dehydrogenase, biology.protein, Neurology (clinical), Sciatic nerve
Published
1963

2. Intracerebral distribution pattern of radioactive morphine and morphine-like drugs after intraventricular and intrathecal injection

Author

Albert Herz, Georg W. Kreutzberg, Peter Schubert, and Hansjörg Teschemacher

Subjects
Drug, Histology, Chemistry, media_common.quotation_subject, Dihydromorphine, Cell Biology, Drug action, Pharmacology, Intrathecal, Fentanyl, White matter, Medical Laboratory Technology, medicine.anatomical_structure, Anesthesia, medicine, Morphine, Distribution (pharmacology), Molecular Biology, media_common, medicine.drug
Abstract

The intracerebral distribution patterns of 14C-morphine, 3H-dihydromorphine, and 3H-fentanyl after intraventricular injection were studied autoradiographically in rats and rabbits. The extent of permeation into the ventricular wall was measured at different times after injection. The hydrophilic morphine and dihydromorphine could be demonstrated within the tissue up to 4 hours. They seemed to be retained within the gray matter and hindered in crossing fiber bundles. On the other hand, the lipophilic fentanyl was quickly removed from the brain but remained relatively longer demonstrable within the white matter. Also, after intrathecal injection of 14C-morphine a time dependent spread from the injection site was observed. The use of autoradiography in pharmacological experiments as described was found advantageous. Thus, it is possible to correlate directly, the time course of the pharmacological effect and the respective distribution pattern of the drug applied. This may lead to better information about the probable sites of drug action.

Published
1970

3. Beitrag zur Enzymhistochemie und Gewebekultur des Ganglioneuroms

Author

Erich Langer, Margarete Minauf, and Georg W. Kreutzberg

Subjects
Cellular and Molecular Neuroscience, Chemistry, Neurology (clinical), Molecular biology, Pathology and Forensic Medicine
Abstract

Enzymhistochemische Untersuchungen und die Gewebekultur eines unterschiedlich differenzierten Ganglioneuroms des hinteren Mediastinums, das histologisch durch vielfaltige Reifungsstufen der Ganglienzellen gekennzeichnet war, fuhrte zu folgenden Ergebnissen: Die Aktivitat der unspezifischen Esterase, der sauren Phosphatase und oxydativer Enzyme gibt quantitativ eine grose Variabilitat in den unterschiedlich differenzierten Zellen. Die reifen Ganglienzellen besitzen starkste, die Neuroblasten und andere Vorstufen der Nervenzellen nur schwache Enzymaktivitaten. Das Vorkommen von Acetylcholinesterase sowie die relativ starke Aktivitat der Enzyme des Zitronensaurecyclus und anderer Dehydrogenasen last vermuten, das der Stoffwechsel der differenzierten Ganglienzellen des Tumors nicht wesentlich von dem normaler Nervenzellen abweicht. In der Gewebekultur kam es wahrend der ersten Woche zur Proliferation mesenchymaler Zellen und zum Auswachsen von Fasern, die sich mit Silber nach der Methode vonBodian impragnieren liesen und deshalb als Nervenfasern gedeutet werden.

Published
1965

5. Neuroplasmic transport in dendrites: effect of colchicine on morphology and physiology of motoneurones in the cat

Author

H.D. Lux, Georg W. Kreutzberg, and Peter Schubert

Subjects
Electrophoresis, Glycine, Nerve Tissue Proteins, Stimulation, Biology, Tritium, Axonal Transport, Microtubules, chemistry.chemical_compound, Microelectrophoresis, Animals, Colchicine, Molecular Biology, Motor Neurons, Lysine, General Neuroscience, Dendrites, Electrophysiology, Spinal Cord, nervous system, Biochemistry, chemistry, Dendritic transport, Cats, Axoplasmic transport, Biophysics, Autoradiography, Neurology (clinical), Orthodromic, Intracellular, Developmental Biology
Abstract

Intracellular application of tritiated amino acids was performed by microelectrophoresis of single lumbar motoneurones in the cat. Incorporation and transport of the radiochemicals were studied in autoradiographs. Simultaneous recording of the electrophysiological activity permitted direct correlation of biochemical, morphological and physiological parameters in the same individual neurone. This technique was used to test the effect of colchicine. The drug produced severe morphological changes but did not significantly influence amino acid incorporation. But transport of proteins, especially in the dendrites, was impaired. This suggest that dendritic transport depends on the integrity of the neurotubular system. Despite severe morphological changes the neurones retained normal resting potentials and generated spikes when stimulated antidromically. Yet, the majority of the neurones do not respond to orthodromic stimulation. This suggests colchicine-induced structural changes of the presynaptic side.

Published
1972

7. Enzymhistochemie menschlicher Hirntumoren und ihrer Gewebekultur

Author

Peter Schubert and Georg W. Kreutzberg

Subjects
Medical Laboratory Technology, Histology, Chemistry, Cell Biology, Molecular Biology, Molecular biology
Abstract

Aus einem Biopsiematerial von etwa 560 Hirntumoren wurden 10 Ependymome, von denen 7 in vitro erfolgreich gezuchtet werden konnten, histologisch und histochemisch untersucht. In vitro war es unter gunstigen Wachstumsbedingungen zu einer Proliferation uniformer epithelahnlicher Zellen gekommen, die an Grose zur Peripherie des Explantates hin gleichmasig zunahmen und mitunter zu Rosettenbildung neigten. Fur die untersuchten oxydativen Enzyme lies sich nach Reaktionsintensitat geordnet im Schnittpraparat und in der Kultur folgende Reihenfolge aufstellen: NADH-R, LDH, NADPH-R, GADH, SDH, Zyt.-Ox.

Published
1967

8. Das Gliom des Opticus

Author

Filippo Gullotta and Georg W. Kreutzberg

Subjects
Gynecology, Cellular and Molecular Neuroscience, medicine.medical_specialty, medicine, Neurology (clinical), Biology, Pathology and Forensic Medicine
Abstract

Es wird uber die erfolgreiche Zuchtung und vergleichende enzymhistochemische Untersuchung eines Opticusspongioblastoms berichtet. Im frisch eingefrorenen Material enthielten die grostenteils spindelformigen Zellen hohe Aktivitaten von DPN-Diaphorase und Lactatdehydrogenase. Die Fermente des Citronensaurecyclus waren nur schwach oder wie die Succinodehydrogenase nicht nachweisbar. Die gleichen enzymhistochemischen Befunde wurden an den Gewebekulturen erhoben. Das Wachstum des “Opticusglioms” entsprach in allem den vonKersting gegebenen Kriterien fur das Spongioblastom des Kleinhirns. Wir mochten uns deshalb der Meinung vonZulch u.a. anschliesen und den Tumor den Spongioblastomen zuordnen. Im Verlaufe der Zuchtung gelang es, das Auftreten von Rosenthalschen Fasern zu verfolgen. Sie liesen sich als Degenerationsprodukte der Geschwulstzellen identifizieren und enthielten keinerlei oxydative Enzyme.

Published
1963

9. Intradendritic transport to postsynaptic sites

Author

Peter Schubert, Georg W. Kreutzberg, L. Toth, and E. Rieske

Subjects
Electrophoresis, Orotic Acid, Glucosamine, Chemistry, General Neuroscience, Guinea Pigs, Synaptic Membranes, Nerve Tissue Proteins, Dendrites, Axonal Transport, Choline, Rats, Microscopy, Electron, Postsynaptic potential, Acetylcholinesterase, Animals, Autoradiography, Neurology (clinical), Amino Acids, Molecular Biology, Neuroscience, Developmental Biology, Fucose
Published
1973

10. Exogenous acetylcholinesterase as tracer for extracellular pathways in the brain

Author

Hisanobu Kaiya and Georg W. Kreutzberg

Subjects
Histology, Time Factors, Aché, Guinea Pigs, Endogeny, Biology, Basement Membrane, Lateral ventricles, chemistry.chemical_compound, Species Specificity, Extracellular, medicine, Animals, Molecular Biology, chemistry.chemical_classification, Basement membrane, Brain, Cell Biology, General Medicine, biology.organism_classification, Acetylcholinesterase, language.human_language, Electric eel, Rats, Medical Laboratory Technology, Microscopy, Electron, Enzyme, medicine.anatomical_structure, chemistry, Biochemistry, Glutaral, Electrophorus, language, Biophysics, Anatomy, General Agricultural and Biological Sciences
Abstract

Exogenous acetylcholinesterase from the electric eel has been used to trace extracellular pathways of the brain. After pretreatment with DFP, which inhibits irreversibly the endogenous AChE, the enzyme is injected into the brain tissue or the lateral ventricles. From the site of injection the enzyme spreads into the brain along extracellular pathway, so that the extracellular space can be demonstrated easily with this method. AChE shows special affinity towards basal laminae (basement membrane) constituents.

Published
1974

11. Maintenance of synaptic membranes by the fast axonal flow

Author

V. Krygier-Bre´vart, Peter Schubert, Dieter Weiss, Georg W. Kreutzberg, and E. Mehl

Subjects
Sucrose, Time Factors, Nerve Tissue Proteins, Biology, Lateral geniculate nucleus, Tritium, Synaptic vesicle, Axonal Transport, Synapse, chemistry.chemical_compound, Biosynthesis, Leucine, Microsomes, Centrifugation, Density Gradient, Animals, Molecular Biology, Myelin Sheath, Visual Cortex, General Neuroscience, Geniculate Bodies, Biological Transport, Mitochondria, Electrophoresis, Microscopy, Electron, Biochemistry, chemistry, Synapses, Biophysics, Microsome, Autoradiography, Specific activity, Electrophoresis, Polyacrylamide Gel, Neurology (clinical), Rabbits, Synaptic Vesicles, Developmental Biology, Synaptosomes
Abstract

Following injection of [3H]leucine into the striate cortex of the rabbit, the nature of the material transported with the fast axonal flow to the lateral geniculate nucleus was investigated. The system proved to be especially suited for biochemical analysis of the transported proteins since interference by local biosynthesis was virtually nil as shown by autoradiography. SDS-gel electrophoresis of the solubilized lateral geniculate nucleus revealed the same complex radioactive protein profiles at 7 and 24 h after injection. The majority of proteins ranged from 50,000 to 75,000 and from 80,000 to 120,000 daltons. The relative specific activity of the synaptic vesicle and microsomal fraction was very low. By far, the highest incorporation of radioactive proteins was found in the fractions containing external synaptosomal membranes. The results further demonstrate the importance of the fast axonal flow for the structural and functional maintenance of the synapse.

Published
1974

12. Axonal transport of adenosine and uridine derivatives and transfer to postsynaptic neurons

Author

Georg W. Kreutzberg and Peter Schubert

Subjects
Adenosine, Chemistry, General Neuroscience, Geniculate Bodies, Biological Transport, Axonal Transport, Uridine, Axons, chemistry.chemical_compound, Postsynaptic potential, Synapses, medicine, Biophysics, Axoplasmic transport, Dactinomycin, Animals, Autoradiography, Neurology (clinical), Rabbits, Molecular Biology, Developmental Biology, medicine.drug, Visual Cortex
Published
1974

13. Results

Author

Gopal D. Das and Georg W. Kreutzberg

Published
1968

14. Summary

Author

Gopal D. Das and Georg W. Kreutzberg

Published
1968

15. Third International Congress of Histochemistry and Cytochemistry

Author

J. E. Kephart, Zenon Steplewski, Kokichi Kanazawa, F. Cisotti, M. P. Ehrlich, K. Holubar, D. F. Swaab, Erich Künzel, J. Thomas Danzi, Erna Avrin, L. Konopska, B. Korych, M. T. Tchaika, Donald W. Misch, Peter W. Neurath, M. M. Coombs, N. H. Bass, Gianfranco Ballare, J. D. Vigario, A. Tixier-Vidal, J. W. Anderson, Peter H. Bartels, Floyd E. Bloom, J. M. S. Prewitt, W. Gössner, V. D. Novikov, Masanori Takeda, A. S. Vecher, Gunter F. Bahr, A. Stammler, Ellen M. Rasch, R. Thalmann, Vick Williams, Lois W. Tice, N. T. Raikhlin, Torbjörn Caspersson, Gianmario Mariuzzi, M. M. Salpeter, Jacques Lipetz, Franca Porcelli, V. Preto Parvis, P. B. Gahan, Hans-Jürgen Herrmann, Karoly Balogh, F. L. Schuster, Z. Lojda, Frederick T. Zugibe, B. Hershenov, Moshe Kalina, Heinz Von Mayersbach, Gordon L. Rosene, C. C. Orton, Jan Wieckowski, M. P. King, Charles E. Hughes, A. L. Bastos, A. A. Castellani, E. E. McGarry, G. Szegly, George L. Wied, Lawrence S. Hurwitz, Jerome P. Horwitz, L. K. Sharashidze, George B. Koelle, Sin Hang Lee, G. C. Budd, Gerhard Müller, A. Björklund, Hilde E. Hirsch, Carlo Alberto Beltrami, R. Håkanson, L. D. Lukyanova, G. G. Glenner, J. Drukker, M. Z. M. Ibrahim, Doina Onicescu, Cornelia Prundeanu, N. V. Samosudova, J. A. F. Silva, S. O. Enenko, C. Pilgrim, Takaharu Matsumoto, Masa-Oki Yamada, Allen Costoff, Peter V. Moulder, F. Patocka, P. Tournier, Bo Thorell, Alberto Candiotti, Vinci Mizuhira, Carl Ritter, A. Simard, Donald R. Hodges, S. Guha, M. I. Parfanovich, D. Bootsma, Wesley J. Birge, J. Hartman, Lloyd J. Roth, Sidney Goldfischer, Blake W. Moore, R. Rigler, M. Rizzotti, M. S. Aksyutina, Lucien J. Rubinstein, William J. Dougherty, V. R. Switsur, Richard M. Torack, B. Perry, Paul J. McMillan, Hélio B. Coutinho, B. Ehinger, Leonard F. Bélanger, K. H. Jaeger, O. Macovschi, D. Zaccheo, Stefan Hovmark, Andre Gerard, Robert S. Ledley, Ferdinando Rossi, R. J. Barrnett, Herbert A. Lubs, J. E. Jirasek, Harold Koenig, M. Stroun, B. Sylvén, V. D. Arutyunov, Richard L. Tyndall, Elisabeth Von der Muehll, X. Yataganas, Brian H. Mayall, J. C. Liu, A. Pope, P. X. Callahan, Susan P. Gaffney, Ei-ichi Yokomura, E. A. Kirianova, L. Monus, Fritz Ruch, Josef V. Freisler, A. M. Terrinha, A. Heller, B. Gabara, Peter J. Stoward, David S. Alkek, G. Kiefer, Alfredo Mariano Garcia, H. H. Hess, Gottfried Werner, Alessandra Forni, Josef Vittek, G. A. Jurzina, P. E. Norgren, H. G. Goslar, Ulrich Welsch, Margaret C. Boadle, Katsumi Wakabayashi, Jean McLean, P. Grigoriadis, Jean R. Adams, C. Bode, M. M. Ogieveckaja, Joseph Rigatuso, Tadao Takeuchi, John E. Pauly, Kendall Preston, Patricia D. Wilson, A. G. E. Pearse, David M. Maeir, T. Abe, O. S. Frankfurt, Jan L. E. Ericsson, Karel Philippens, Gy. Vadasz, E. T. Angelakos, F. Hernández, Frank H. Ruddle, Sally B. Fand, Richard Heitsch, Hideo Takamatsu, M. Matsuda, L. Ya. Yablonovskaya, Wiecher H. VanHouten, Hildegard E. Enesco, I. Törö, Takanori Amakawa, T. J. Conway, M. J. Olszewska, V. E. Shungskaya, Joh. Koudstaal, R. S. Morgan, C. Vladescu, Boris Gueft, A. Urano, Viviane Maggi, Totada R. Shantha, Nicolas Zenker, Lawrence E. Scheving, A. Goldstone, E. N. Sarch, Dean E. Abrahamson, K. Felgenhauer, Thomas B. Shows, J. P. Mitchell, Jeffrey P. Chang, A. A. Masko, Ovidio Duarte-Escalante, L. Roizin, Klaus Wächtler, Lenore Wagner, C. P. Leblond, M. L. Mendelsohn, L. Luksys, K. C. Tsou, Erik Zeuthen, M. Kazama, Helen P. Sorokin, K. A. Gornak, Jácia T. Rocha, T. H. Schiebler, C. W. Goodwin, L. M. Franks, K. Wolff, Sun K. Song, Bun-Ichi Tamaoki, D. A. Sakharov, W. King Engel, Shinichi Imura, Rachele Alberti, J. Ken McDonald, V. Áubrechtova, Patricia A. Kinnison, Yoshihiro Hamashima, Z. A. Cohn, Stanley Scher, C. E. Grossi, J. Jos, Arline D. Deitch, N. V. Donskikh, J.-E. Edström, E. Bajusz, M. F. Kramer, Seth W. Gilkerson, H. Benoit, M. A. Van Dilla, G. Freytag, G. Aureli, E. J. Dupraw, H. R. Tyagi, A. Jarrett, A. I. Bukhonova, C. A. Squier, B. Daneholt, Sunao Iwata, K. Yokoyama, Giovanni Lanza, Margaret S. Misch, F. Gregoire, M. Kage, Ryuei Maeda, Eleanor Rita Lappano-Colletta, L. A. Kamentsky, S-U. Kim, U. M. Gross, Halina Sierakowska, M. Dauwalder, Patricia L. Haley, Shunta Hirose, Dorothy J. Moore, E. B. Masurovsky, Firoze B. Jungalwala, Walter E. Stumpf, Howard I. Siegel, M. Chyle, C. Balduini, A. L. Shnaper, Kei-Ichi Hirai, R. E. Smith, M. Serban, J. R. Coulter, A.-M. Bullens, Voldemar Vilter, Ph. J. Hoedemaeker, N. I. Tsirelnikov, Ines Mandl, K. I. Predkel, Dwo Lynm, Shigeru Morikawa, L. V. Danilova, Masasuke Akamatsu, J. Bolubasz, J. C. Beck, Amour F. Liber, Hans-Georg Schiemer, Peter F. Schatzki, J. D. Elema, J. Bernsohn, N. A. Probatova, Barbara Penar, Masando Hayashi, Tibor Barka, G. Gahrton, Annunzia Fraschini, U. Filotto, Paul F. Doolin, G. Fabris, Sergei P. Sorokin, Mildred Y. Chang, H. Dariush Fahimi, Mitsuo Sasaki, Nina D. Gracheva, Tinrat Stitnimankarn, T. Adesanya Ige Grillo, Gösta Jonsson, Regina Leske, A. Huszczuk, Sophia Kakari, W. Straus, J. Martinez De Morentin, M. F. Sheff, Frank Wohlrab, Timothy C. Appleton, T. Gilbert Scott, Eric A. Barnard, N. S. Sturua, Maria Popescu, Junichi Totsu, Takuma Saito, Lew Cunningham, Benito Monis, Ch. Owman, L. Ya. Lomakina, Alvin M. Burt, Z. Lodin, Satimaru Seno, K. P. M. Heirwegh, Giuseppe Gerzeli, Elliott F. Osserman, K. Nakashio, E. Brodie, W. Sandritter, M. H. Himes, John Pryse-Davis, R. S. Santti, Benjamin F. Trump, Nencioni T, B. Falck, Shinsuke Kanamura, Benjamin F. Jales, Attila Tanyolac, Konrad Akert, Eugene H. Blackstone, Donald B. Fisher, Theodore Peters, Zay B. Curtis, C. Mittermayer, B. Sporrong, Kazuko Uchida, Mortimer L. Mendelsohn, Daniel S. Friend, Yasuo Kishino, William D. Selles, A. V. Sakharova, Hazel Cheng, R. Daoust, Vernon J. Perez, W. Gepts, Stanley Ellis, Philip Ohringer, S. A. Burnasheva, J. E. Scott, A. Ingrami, M. J. Hardonk, Yvonne Hosannah, Carlton E. Blackwood, Jerome P. Miksche, Ludmila Fotin, S. Matsukawa, Otfried Müller, A. M. Baptista, V. Genta, P. F. Mullaney, Antti U. Arstila, G. Mori, H. Goebell, M. Ya. Subbotin, H. Kim, Niel Wald, E. Pascoe, Harold J. Sobel, Hideo Shindo, Merton Sandler, Richard Cornell, A. Riva, Sten W. Jakobsson, R. G. J. Willighagen, P. Sadauskas, V. J. Desmet, Roy O. Weller, Bjarne Pearson, L. Kalevi Korhonen, J. P. Waterhouse, Bernard Droz, Frank S. LaBella, H. Galjaard, C. L. Rosales, Andrzej Vorbrodt, Gerard M. Lehrer, Barry W. Wilson, Maria Gabriella, Stanley G. Sawicki, P. Leth Jørgensen, Nobuo Ohuchi, M. Bradshaw, Ju. J. Tendetnik, S. Virág, Norman G. Anderson, C. Burdick, Alex B. Novikoff, Christopher ’Seinde Adeoye, M. Zamfirescu-Gheorghiu, A. Gropp, K. D. Rokhlenko, H. Ooms, Eli Shuter, J. S. Hugon, V. V. Terskikh, C. Bona, Anne B. Johnson, J. M. Nunes, D. M. Conning, Nobutaka Itoh, S. R. Pelc, Z. Chirulescu, Walter C. Schneider, H. Kobayashi, Sohan L. Manocha, John R. Esterly, Marilyn G. Farquhar, O. E. Rjadneva, J. Lindner, N. G. Stepanova, N. N. Shiukashvili, Russell Ranshaw, A. Schauer, Väinö K. Hopsu-Havu, A. W. Carbonell, George B. Jerzy Glass, F. M. Matschinsky, Moshe Wolman, Edgar E. Smith, P. Satir, Claudia Joandrea-Casian, J. Tappeiner, Hans E. Kaiser, Fran Morriss, K. Grasedyck, J. F. Jongkind, Murray B. Bornstein, Winifred W. Doane, K. D. Eristawi, Giovanni Prenna, L. Matturri, Alexander M. Rutenburg, J. R. Garrett, J. Svejda, Muneaki Abe, B. Lederer, Waine C. Johnson, F. W. D. Rost, J. M. Hewitt, K. P. Ganina, Judith M. S. Prewitt, M. B. Kalamkarova, Robert M. Rosenbaum, Sheldon C. Sommers, Alfred C. Standen, W. G. Whaley, A. V. Zelenin, Paul K. Nakane, N. R. Ringertz, Robert Lev, L. L. Fadeeva, J. De Groote, Jacob S. Hanker, N. N. Sokolov, Jürgen Schlüns, V. I. Malyuk, William Bennett, Italo Nenci, E. O. Riecken, Milan R. Henzl, J. P. Fouquet, Vincent Spitaleri, Frank H. Moyer, T. John Leppi, P. Anker, Chandicharan Deb, Tomichi Masuya, L. Bolund, H. G. Godlewski, A. M. Souza Toledo, Jacques de Graef, Robert Katzman, David Glick, Eli Robins, Horst Zimmermann, Premysl Fric, P. J. Melnick, R. Nayak, S. Stroman, C. W. M. Adams, Charles A. Ashley, Teresa I. Mercado, Margaret E. Long, L. Glismann, D. J. Marshall, C. Velican, Gy. Rappay, Falls B. Hershey, M. Bergeron, A. S. Petrova, Ian T. Young, V. N. Reshetnikov, M. van der Ploeg, I. Diculescu, M. G. Manfredi Romanini, Godfrey C. Hoskins, Henri G. Vetter, Maurice Sandler, Takayuki Harada, E. Bacsy, Robert C. Allen, A. J. Hale, O. I. Epifanova, Geoffrey H. Bourne, Hisao Yamaguchi, Antti Penttilä, P. van Duijn, K. D. Barron, Michio Yamamoto, Georg W. Kreutzberg, D. S. Van Fleet, Akira Mizutani, William H. Fishman, Irmin Sternlieb, Thomas H. Roderick, M. R. Murray, G. Johannes, Theodore A. Wilcox, L. Silverman, J. W. Fischbein, A. H. Koeppen, N. O. Jacobsen, B. M. Pollock, E. S. Ruchadse, W. B. Quay, Victor Dubowitz, Janusz Komender, S. I. Zacks, Paul J. Anderson, Annette Herscovics, H. B. Tewari, J. Filkuka, O. K. Langley, W. H. McShan, E. Birch, Philip Pizzolato, Francesco Della Corte, U. A. Gabunia, Eric Holtzman, Yoshihisa Morizono, Nobuo Ihara, L. Vollrath, L. Guerra, Ezio Giacobini, Jose J. Bubis, S. Curri, M. Borgers, C. Vendrely, A. Lageron, A. Weinstock, Morris J. Karnovsky, Arnold Vogt, V. Dabkevcius, P. N. Dean, B. B. Fuchs, McCormick Templeton, M. A. Presnov, E. Thomas, L. P. Lipchina, D. C. Livingston, D. Killander, Maffo Vialli, Doina Velican, M. Guigon, Dante Marques, Paul L. Wolf, L. C. Junqueira, Bette Seamond, Frederick H. Kasten, J. Srajer, Günter Steinbach, H. H. Benitez, J. F. Lhotka, J. Merzel, Arnold M. Seligman, M. P. Viola-Magni, C. L. Rutherford, E. Szabo, R. D. Lillie, N. J. Nadler, Walter Chavin, Masao Yamamura, A. Lauria, Regina O’Brien, F. L. Leites, N. Marcus, Janice Vazquez, Gabriel C. Godman, C. Poort, R. Barer, Friedrich A. O. Eckner, Daniel Roth, Ludwig Sternberger, Ikuo Suesada, and O. N. Berezina

Subjects
0303 health sciences, Pathology, medicine.medical_specialty, biology, 030302 biochemistry & molecular biology, Acridine orange, Acid phosphatase, Chronic gastritis, Intestinal metaplasia, medicine.disease, Small intestine, 03 medical and health sciences, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, International congress, medicine, Cytochemistry, biology.protein, Immunohistochemistry, 030304 developmental biology
Abstract

The tissue distribution of enzymatic activities in intestinal metaplasia stomachs exhibiting chronic gastritis was compared histochemically with that of the small intestine in man.

Published
1968

16. Selective uptake of 3H-6-hydroxydopamine by neurones of the central nervous system

Author

Peter Schubert, Georg W. Kreutzberg, K. Reinhold, and Albert Herz

Subjects
Central Nervous System, Neurons, Pathology, medicine.medical_specialty, Hydroxydopamine, Frozen section procedure, Chemistry, General Neuroscience, Central nervous system, Biological Transport, Active, Anatomy, Tritium, Cerebral Ventricles, Rats, Hydroxydopamines, medicine.anatomical_structure, Mesencephalon, Ventricle, Freezing, medicine, Animals, Autoradiography, Rabbits, Caudate Nucleus
Abstract

Tritiated 6-hydroxydopamine (6-OHDA) was injected into the lateral ventricle of rats and rabbits and visualized by means of autoradiography. In unfixed frozen sections, radioactive material was found penetrating about 2 mm into the periventricular tissue within 4–48 hours after injection. Radioactivity was localized preferably in particular nuclei, leaving the fiber bundles nearly free of label.

Published
1973

17. Third International Congress of Histochemistry and Cytochemistry

Author

P. Tournier, Bo Thorell, F. Cisotti, Nicolas Zenker, M. P. Ehrlich, K. Holubar, Jose J. Bubis, J. Fischbein, Shinichi Imura, S. Curri, Susan P. Gaffney, Ellen M. Rasch, Totada R. Shantha, Lawrence E. Scheving, Hirose Shunta, G. Freytag, U. M. Gross, M. Borgers, G. Fabris, D. A. Sakharov, G. C. Budd, M. F. Kramer, C. Vendrely, A. Lageron, V. K. Hopsu-Havu, A. Weinstock, D. F. Swaab, Arnold Vogt, J. A. F. Silva, J. F. Jongkind, Murray B. Bornstein, B. Lederer, Zay B. Curtis, B. Sporrong, S. O. Enenko, Kazuko Uchida, P. N. Dean, F. W. D. Rost, B. B. Fuchs, M. Kage, M. B. Kalamkarova, Erna Avrin, William Bennett, M. J. Hardonk, K. H. Jaeger, McCormick Templeton, Z. Lojda, M. Chyle, C. A. Squier, M. T. Tchaika, Paul K. Nakane, Firoze B. Jungalwala, O. N. Berezina, Italo Nenci, E. O. Riecken, J. E. Kephart, Zenon Steplewski, Halina Slerakowska, M. M. Coombs, Joandrea-Casian Claudia, L. L. Fadeeva, J. De Groote, Sergei P. Sorokin, M. A. Presnov, Fritz Ruch, Milan R. Henzl, Ellis Stanley, Lloyd J. Roth, Masasuke Akamatsu, Eleanor Rita Lappano-Colletta, J. P. Fouquet, N. I. Tsirelnikov, K. I. Predkel, Dwo Lynm, L. Konopska, R. Rigler, A. Tixier-Vidal, U. Filotto, Paul F. Doolin, G. Gahrton, Junichi Totsu, M. Wolman, L. V. Danilova, Vincent Spitaleri, Takuma Saito, T. Adesanya Ige Grillo, W. Gössner, Katsumi Wakabayashi, J. J. Bubis, E. Thomas, W. Straus, J. Martinez De Morentin, Shinsuke Kanamura, Harold Koenig, P. X. Callahan, A. S. Vecher, Tinrat Stitnimankarn, Eugene H. Blackstone, E. N. Sarch, Dean E. Abrahamson, Thomas B. Shows, J. P. Mitchell, Droz Bernard, Takanori Amakawa, F. Hernández, Frank H. Moyer, Hazel Cheng, R. Daoust, Forni Alessandra, Josef Vittek, F. L. Schuster, Paul L. Wolf, L. C. Junqueira, Bette Seamond, Alex B. Novikoff, Tadao Takeuchi, J. Tappeiner, Abe Muneaki, E. Pascoe, A. M. Rutenburg, Harold J. Sobel, Jean R. Adams, P. Grigoriadis, Patricia D. Wilson, A. G. E. Pearse, A. Pope, Hideo Shindo, Frederick H. Kasten, Heinz Von Mayersbach, Gordon L. Rosene, L. D. Lukyanova, G. G. Glenner, N. H. Bass, K. Nakashio, Gianfranco Ballare, R. Thalmann, George B. Koelle, Jeffre Y. P. Chang, Frank H. Ruddle, John Pryse-Davis, Jerome P. Miksche, R. S. Santti, M. Ya. Subbotin, J. Srajer, Günter Steinbach, Chandicharan Deb, Tomichi Masuya, R. G. J. Willighagen, L. Bolund, H. G. Godlewski, Gerzeli Giuseppe, A. Gropp, Sin Hang Lee, Seth W. Gilkerson, M. A. Van Dilla, C. Burdick, J. D. Vigario, Hilde E. Hirsch, Stanley Ellis, Carlo Alberto Beltrami, Philip Ohringer, Erik Zeuthen, G. Szegly, M. Kazama, Helen P. Sorokin, J. W. Anderson, R. Håkanson, Merton Sandler, O. S. Frankfurt, H. H. Benitez, C. Pilgrim, J. Lindner, Väinö K. Hopsu-Havu, Peter H. Bartels, J. P. Waterhouse, Bernard Droz, Floyd E. Bloom, Masa-Oki Yamada, B. Perry, Paul J. McMillan, Allen Costoff, A. A. Masko, Takaharu Matsumoto, H. Benoit, G. M. Mariuzzi, F. Patocka, Joh. Koudstaal, McDonald J. Ken, J. Ken McDonald, J. M. S. Prewitt, H. R. Tyagi, Hélio B. Coutinho, Rachele Alberti, M. Stroun, E. B. Masurovsky, Jacques Lipetz, V. D. Novikov, G. Gries, Wiecher H. Van Houten, Balogh Kåroly, Patricia L. Haley, O. Macovschi, S-U. Kim, C. Vladescu, I. Törö, V. E. Shungskaya, Wesley J. Birge, Ines Mandl, C. P. Leblond, Wald Niel, Shigeru Morikawa, Dariush H. Fahimi, Anne B. Johnson, J. E. Jirasek, T. H. Schiebler, H. Kobayashi, C. L. Rosales, A. Jarrett, K. Wolff, Nina D. Gracheva, Gösta Jonsson, Hans-Georg Schiemer, Torbjörn Caspersson, K. P. M. Heirwegh, D. Zaccheo, M. M. Salpeter, L. Monus, E. T. Angelakos, Marilyn G. Farquhar, Gy. Vadasz, Franca Porcelli, K. Yokoyama, M. J. Olszewska, H. Koenig, A. L. Shnaper, A. M. Terrinha, L. P. Lipchina, Ryuei Maeda, Ulrich Welsch, Peter J. Stoward, David S. Alkek, Kalevi L. Korhonen, Jan Wieckowski, Mitsuo Sasaki, M. L. Mendelsohn, Maggi Viviane, L. Luksys, Satimaru Seno, A. M. Souza Toledo, Herbert A. Lubs, Josef V. Freisler, Sophia Kakari, C. Mittermayer, N. A. Probatova, Elisabeth Von der Muehll, David M. Maeir, Jan L. E. Ericsson, Karel Philippens, D. C. Livingston, M. Kalina, J. Drukker, L. Ya. Lomakina, Alvin M. Burt, Z. Lodin, J. C. Liu, G. Kiefer, H. G. Goslar, V. Áubrechtova, Patricia A. Kinnison, A. Heller, E. Brodie, Eli Robins, K. C. Tsou, Z. A. Cohn, Stanley Scher, E. Bajusz, Attila Tanyolac, Theodore Peters, Cornell Richard, D. Killander, John E. Pauly, Vernon J. Perez, Maffo Vialli, J. Hartman, K. D. Rokhlenko, H. Ooms, M. Serban, Voldemar Vilter, Margaret C. Boadle, Jean McLean, M. M. Ogieveckaja, C. W. Goodwin, W. Sandritter, M. H. Himes, William D. Selles, A. V. Sakharova, A. Ingrami, A.-M. Bullens, Roy O. Weller, B. Hershenov, Lenore Wagner, Doina Velican, P. B. Gahan, Brian H. Mayall, G. A. Jurzina, Blake W. Moore, P. E. Norgren, Hildegard E. Enesco, Ju. J. Tendetnik, S. Virág, Norman G. Anderson, Carlton E. Blackwood, Annunzia Fraschini, Boris Gueft, A. Goldstone, Mildred Y. Chang, Nobutaka Itoh, Ch. Owman, Della Corte Francesco, Sally B. Fand, M. Guigon, Daniel S. Friend, Howard I. Siegel, T. Gilbert Scott, B. Ehinger, Donald B. Fisher, K. Felgenhauer, J. S. Hugon, Mortimer L. Mendelsohn, Benito Monis, C. Bode, Richard M. Torack, A. Riva, Elliott F. Osserman, A. A. Castellani, A. Simard, Donald R. Hodges, Dante Marques, Leonard F. Bélanger, A. Urano, B. Thorell, Giacobini Ezio, S. A. Burnasheva, A. Schauer, B. Korych, V. V. Terskikh, Klaus Wächtler, S. Guha, M. I. Parfanovich, D. Bootsma, H. Kim, Richard L. Tyndall, N. G. Stepanova, N. N. Shiukashvili, M. Rizzotti, X. Yataganas, J. M. Nunes, M. F. Sheff, Waine C. Johnson, J. E. Scott, Sun K. Song, O‘brien Regina, G. E. Mazza, J. M. Hewitt, Robert M. Rosenbaum, Andre Gerard, Robert S. Ledley, Ferdinando Rossi, Frank S. LaBella, Christopher ’Seinde Adeoye, B. Sylvén, V. D. Arutyunov, Moshe Kalina, Sheldon C. Sommers, P. Sadauskas, Kishino Yasuo, M. Dauwalder, Alfredo Mariano Garcia, H. H. Hess, V. J. Desmet, H. Galjaard, O. E. Rjadneva, Benjamin F. Jales, Ei-ichi Yokomura, L. Roizin, Erich Künzel, J. Thomas Danzi, Peter V. Moulder, Konrad Akert, Moshe Wolman, M. Zamfirescu-Gheorghiu, J. Bolubasz, J. C. Beck, Amour F. Liber, B. Daneholt, Donald W. Misch, Peter W. Neurath, Masanori Takeda, Arline D. Deitch, C. Bona, Alberto Candiotti, Yvonne Hosannah, Sunao Iwata, Giovanni Lanza, Winifred W. Doane, K. D. Eristawi, Giovanni Prenna, P. F. Mullaney, Vinci Mizuhira, Sohan L. Manocha, John R. Esterly, L. Matturri, J. R. Garrett, Sidney Goldfischer, A. Stammler, Antti U. Arstila, George B. Jerzy Glass, A. W. Carbonell, Stefan Hovmark, F. M. Matschinsky, Hans E. Kaiser, K. P. Ganina, L. K. Sharashidze, Judith M. S. Prewitt, Barbara Penar, Edgar E. Smith, A. L. Bastos, Dorothy J. Moore, Gerhard Müller, A. Björklund, E. A. Kirianova, Jürgen Schlüns, Eric A. Barnard, N. S. Sturua, Maria Popescu, Bjarne Pearson, Kokichi Kanazawa, N. N. Sokolov, V. Parvis Preto, G. Mori, Regina Leske, Glick David, V. I. Malyuk, R. J. Barrnett, M. Bradshaw, W. Gepts, Lois W. Tice, N. T. Raikhlin, L. M. Franks, P. Anker, Gerard M. Lehrer, Barry W. Wilson, H. Goebell, Hideo Takamatsu, Jacques de Graef, Frederick T. Zugibe, C. E. Grossi, J. Jos, S. R. Pelc, Z. Chirulescu, E. E. McGarry, George L. Wied, A. I. Bukhonova, Walter C. Schneider, Margaret S. Misch, Lawrence S. Hurwitz, M. Z. M. Ibrahim, Doina Onicescu, N. V. Samosudova, Penttilä Antti, Shuter Eli, Premysl Fric, Sten W. Jakobsson, Frank Wohlrab, M. Matsuda, L. Ya. Yablonovskaya, T. Abe, C. Balduini, R. Nayak, S. Stroman, Masando Hayashi, Tibor Barka, A. Huszczuk, C. W. M. Adams, Margaret E. Long, L. Glismann, K. A. Gornak, Jácia T. Rocha, D. J. Marshall, Nobuo Ohuchi, A. S. Petrova, Yoshihiro Hamashima, Henri G. Vetter, J.-E. Edström, Peter F. Schatzki, E. Bacsy, D. S. Van Fleet, William H. Fishman, J. D. Elema, Annette Herscovics, J. Bernsohn, Williams Vick, L. A. Kamentsky, H. B. Tewari, Walter E. Stumpf, Kei-Ichi Hirai, R. E. Smith, Timothy C. Appleton, Benjamin F. Trump, P. Satir, Nencioni T, B. Falck, Fran Morriss, K. Grasedyck, J. Svejda, Alfred C. Standen, W. G. Whaley, A. V. Zelenin, N. R. Ringertz, Hisao Yamaguchi, Jacob S. Hanker, Cornelia Prundeanu, K. D. Barron, B. Gabara, R. S. Morgan, Michio Yamamoto, G. Aureli, E. J. Dupraw, F. Gregoire, J. R. Coulter, V. N. Reshetnikov, M. van der Ploeg, Irmin Sternlieb, Robert C. Allen, A. J. Hale, O. I. Epifanova, P. van Duijn, Thomas H. Roderick, Georg W. Kreutzberg, Robert Katzman, David Glick, Horst Zimmermann, Tamaoki Bun-Ichis, A. H. Koeppen, Herrmann Hans-Jürgen, M. Bergeron, Joseph Rigatuso, Gottfried Werner, Kendall Preston, Richard Heitsch, T. J. Conway, Ovidio Duarte-Escalante, N. V. Donskikh, Ph. J. Hoedemaeker, N. O. Jacobsen, B. M. Pollock, E. S. Ruchadse, P. Leth Jørgensen, Lew Cunningham, D. M. Conning, Theodore A. Wilcox, L. Silverman, G. F. Bahr, J. W. Fischbein, Ian T. Young, Vorbrodt Andrzej, Geoffrey H. Bourne, W. B. Quay, Victor Dubowitz, Janusz Komender, S. I. Zacks, Akira Mizutani, J. Filkuka, O. K. Langley, W. H. McShan, E. Birch, M. R. Murray, Paul J. Anderson, Philip Pizzolato, Yoshihisa Morizono, U. A. Gabunia, Eric Holtzman, Nobuo Ihara, L. Vollrath, S. Matsukawa, Otfried Müller, A. M. Baptista, V. Genta, L. Guerra, Maria Gabriella, Stanley G. Sawicki, Russell Ranshaw, Robert Lev, T. John Leppi, P. J. Melnick, Morris J. Karnovsky, Charles A. Ashley, Fotin Ludmila, V. Dabkevcius, Teresa I. Mercado, C. Velican, Gy. Rappay, Falls B. Hershey, I. Diculescu, M. G. Manfredi Romanini, Godfrey C. Hoskins, Maurice Sandler, Takayuki Harada, G. Johannes, J. F. Lhotka, J. Merzel, Arnold M. Seligman, M. P. Viola-Magni, C. L. Rutherford, E. Szabo, R. D. Lillie, N. J. Nadler, Walter Chavin, Masao Yamamura, A. Lauria, F. L. Leites, N. Marcus, Janice Vazquez, Gabriel C. Godman, C. Poort, R. Barer, Friedrich A. O. Eckner, Daniel Roth, Ludwig Sternberger, Ikuo Suesada, C. C. Orton, M. P. King, Charles E. Hughes, Jerome P. Horwitz, Ritter Carl, M. S. Aksyutina, Lucien J. Rubinstein, William J. Dougherty, and V. R. Switsur

Subjects
0303 health sciences, 03 medical and health sciences, Chemistry, International congress, 030302 biochemistry & molecular biology, Cytochemistry, Anatomy, 030304 developmental biology
Published
1968

18. Volume changes in the axon during regeneration

Author

Peter Schubert and Georg W. Kreutzberg

Subjects
Male, Stylomastoid foramen, Time Factors, Feedback control, Biology, Nerve Fibers, Myelinated, Pathology and Forensic Medicine, Feedback, Cellular and Molecular Neuroscience, medicine, Animals, Axon, Medulla Oblongata, Regeneration (biology), Anatomy, Facial nerve, Axons, Nerve Regeneration, Rats, Facial Nerve, medicine.anatomical_structure, nervous system, Chromatolysis, Medulla oblongata, Axoplasmic transport, Neurology (clinical)
Abstract

Axon diameters have been measured in the intramedullary genu of the facial nerve after cutting the nerve in the periphery at the stylomastoid foramen. Two days after operation decrease of axonal diameters of 11% was observed, and between 10 and 21 days these diameters were decreased by approximately 40%. After the 3rd week p. op. axons regain slowly most of their original size, but a permanent deficit of 13–15% was found 135 to 365 days after operation. The data are discussed in the light of recent findings on enhanced axonal transport during regeneration. A hypothesis is also presented showing conceivable possibilities of a feedback control mechanism informing the neuronal perikaryon on events taking place in the axonal periphery.

Published
1971

19. Displacement of synaptic terminals from regenerating motoneurons by microglial cells

Author

Georg W. Kreutzberg and K. Blinzinger

Subjects
Male, Motor Neurons, Microscopy, Histology, Chemistry, Right facial nerve, Cell Biology, Dendrites, Axonal Reaction, Pathology and Forensic Medicine, law.invention, Nerve Regeneration, Rats, Microscopy, Electron, nervous system, law, Nerve cells, Synapses, Functional significance, Animals, Displacement (orthopedic surgery), Electron microscope, Neuroscience, Process (anatomy), Neuroglia
Abstract

Axonal reaction of motoneurons has been shown to be usually accompanied by an early and brisk proliferation of perineuronal microgliacytes. In order to clarify the real nature of such newly formed microglial satellites and their fine structural relationships to the regenerating nerve cells, facial nuclei from bilateral preparations were examined by light and electron microscopy 4 days after cutting the right facial nerve in rats. On the transected side, microgliacytes could often be observed closely adjoining motoneuron perikarya and main dendrites over long distances, and thereby removing morphologically intact synaptic terminals from the neuronal surface membranes. This displacement of boutons by microglial cells is probably preceded by a loosening of the synaptic contacts due to some unknown membrane changes in the regenerating motoneurons. The functional significance of this considerable deafferentation process could not be entirely elucidated.

Published
1968

20. Excitation and axonal flow: Autoradiographic study on motoneurons intracellularly injected with a 3H-amino acid

Author

H.D. Lux, Peter Schubert, Georg W. Kreutzberg, and A. Globus

Subjects
Time Factors, Glycine, Stimulation, Tritium, Methods, medicine, Animals, Axon, Motor Neurons, chemistry.chemical_classification, Iontophoresis, Chemistry, General Neuroscience, Axons, Electric Stimulation, Antidromic, Amino acid, Electrophysiology, medicine.anatomical_structure, nervous system, Protein Biosynthesis, Cats, Biophysics, Autoradiography, Neuron, Rheology, Neuroscience
Abstract

Tritiated glycine has been injected intracellularly into cat motoneurons by means of cross barrel iontophoresis through microelectrodes. The amino acid is incorporated into proteins in the cell body and the synthesized radioactive proteins have been localized by autoradiography. The single-cell-injection technique has several advantages over the usual intraperitoneal, intravenous or intrathecal injection, e.g. distribution of the radiochemical is strictly confined to the injected neuron; the amount of injected substance can be estimated; it is easy to investigate simultaneously electrophysiological and morphological characteristics of a defined neuron. Since the injected cell body releases radioactive proteins into the axon, the transport of these substances can be studied under various conditions. In this study the influence of antidromic stimulation has been studied. Stimulated motoneurons demonstrate higher radioactivity in their cell bodies than unstimulated ones, reflecting an increased protein synthesis. The differences are even more pronounced in the axons of stimulated cells when compared with unstimulated ones. A significant, higher amount of radioactive material — up to 100% increase as demonstrated by silver grain counting — can be seen in the axons of stimulated neurons. Although considerable differences seem to exist for the quantity of the exported proteins no significant differences have been detected for the velocity of transportation. In axons of stimulated and unstimulated neurons proteins advance toward the periphery at the same rate.

Published
1970

21. Introduction

Author

Gopal D. Das and Georg W. Kreutzberg

Published
1968

22. Enzymhistochemische ver�nderungen in axonen des r�ckenmarks nach durchschneidung der langen bahnen

Author

Georg W. Kreutzberg

Subjects
chemistry.chemical_compound, Neurology, Chemistry, Lactate dehydrogenase, Neurology (clinical), Molecular biology, Malate dehydrogenase
Abstract

Nach Durchschneidung des Ruckenmarks von Ratten wurden nach Uberlebenszeiten zwischen 4 Std und 50 Tagen folgende Enzyme untersucht: DPNH-Tetrazolium-Reduktase, TPNH-Tetrazolium-Reduktase, Succinodehydrogenase, Lactatdehydrogenase, Malatdehydrogenase und Cytochromoxydase. Innerhalb von 24 Std kommt es dabei zu einer eindrucksvollen Steigerung der Aktivitat aller untersuchten Enzyme in den Axonstumpfen sowohl der aufsteigenden als auch der absteigenden Bahnen. Auch finden sich im Fruhstadium diese Veranderungen sowohl in den proximalen als auch in den distalen Stumpfen. Im Randgebiet zeigen wundnahe Abschnitte von Axonen gleichfalls eine erhohte Enzymaktivitat. Die vermehrte Aktivitat oxydativer Enzyme in den proximalen Axonstumpfen halt etwa 5 Tage an und fallt dann rasch ab. In den distalen Stumpfen nimmt sie bereits am 2. Tag wieder ab.

Published
1963

23. Single Cell Isotope Injection Technique, a Tool for Studying Axonal and Dendritic Transport

Author

Peter Schubert, H.D. Lux, and Georg W. Kreutzberg

Subjects
chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Iontophoresis, Dendritic transport, medicine, Biophysics, Choline, Stimulation, Axon, Spinal cord, Intracellular, Antidromic
Abstract

Radioactive precursors (amino acids and choline) were applied to cat spinal motoneurons by means of micro pipette electrophoresis. The amount injected was determined by the iontophoresis current used. Simultaneous recording of the electrical properties of the nerve cells allowed their identification and the control of the site and effect of injection. At different times after injection (4 min to 3 days) the cats were sacrified by formalin perfusion and autoradiographs were prepared from serial sections of the paraplast embedded spinal cord. This technique provides a high concentration of strictly intracellular radioactivity which is advantageous for the study of intracellular transport. The results indicate that proteins most probably synthetized within the nerve cell soma are transported within the dendrites nearly up to their terminals at a rate of at least 3 mm/h and within the axon at different rates of 0.5 and 1.7 mm/h. Following antidromic stimulation the synthesis of proteins within the nerve cell soma and their export into the axon was increased. Choline derivatives thought to be mainly phospholipids were also transported into dendrites and axon.

Published
1971

24. Pathogenetic mechanism of experimentally-induced spongy degeneration

Author

Georg W. Kreutzberg and William W. Carlton

Subjects
Male, Pathology, medicine.medical_specialty, Cerebellum, Subependymal Tissue, Encephalopathy, Vacuole, Biology, Reticular formation, Pathology and Forensic Medicine, White matter, Neurologic Manifestations, Cellular and Molecular Neuroscience, Cerebellar Cortex, medicine, Isoniazid, Animals, Cell Nucleus, Brain Diseases, Reticular Formation, medicine.disease, medicine.anatomical_structure, Ducks, nervous system, Vacuolization, Medulla oblongata, Neurology (clinical), Neuroglia
Abstract

Ducklings fed diets containing INH at level of 0.1% developed in a period of 4 weeks a tissue syndrome which was described previously as a spongy degeneration of the white matter (Carlton andKreutzberg). The formal pathogenesis was the subject of this study. Early changes were observed after 48 hours of administration of 0.15% INH. Severe alterations appeared in astroglia cells of the subependymal tissue and in deep roof nuclei of the cerebellum, cerebellar white matter, cerebellar peduncles and in the reticular formation of the medulla oblongata. Early changes included swelling of cell bodies and nuclei, formation of cytoplasmic vacuoles, and rupture of nuclei. Later, coalesence of vacuoles and rupture of cells resulted in the formation of microcysts creating a status spongiosus. By this process the INH encephalopathy can be defined basically as an oedematous change of the astrocytes followed by a liquefaction of these glia cells and ending in a vacuolization of the affected region simulating the tissue syndrome of spongy degeneration.

Published
1967

25. Electron microscopical demonstration of acid phosphatase activity in the central nervous system

Author

Georg W. Kreutzberg and Hermann Hager

Subjects
Central Nervous System, Histology, Central nervous system, Acid Phosphatase, law.invention, symbols.namesake, Pigment, law, Cricetinae, medicine, Animals, Molecular Biology, Cerebral Cortex, Neurons, biology, Histocytochemistry, Acid phosphatase, Brain, Cell Biology, Cortical neurons, Golgi apparatus, Enzyme assay, Cell biology, Medical Laboratory Technology, Microscopy, Electron, medicine.anatomical_structure, Biochemistry, visual_art, symbols, visual_art.visual_art_medium, biology.protein, Electron microscope, Developmental biology, Neuroglia
Abstract

A modified technique is described for the demonstration of acid phosphatase activity in the central nervous system by means of electron microscopy. Enzyme activity can be demonstrated in lysosomes, pigment bodies, and the Golgi zone of cortical neurons. Glial and endothelial cells also contain acid phosphatase active lysosomes. They are located in the pericarya, and in the processes of the glial cells, respectively.

Published
1966

26. Changes in Axonal Flow during Regeneration of Mammalian Motor Nerves

Author

Georg W. Kreutzberg and Peter Schubert

Subjects
Distal portion, nervous system, Regeneration (biology), Medulla oblongata, Axoplasmic transport, Motor nerve, Anatomy, Biology, Facial nerve, Hypoglossal nerve, Axonal flow
Abstract

Measurements of axonal diameters in regenerating facial nerves of rats indicated a loss of axonal volume of approximately 60% in 14 days. These measurements were made in the intramedullary genu of the facial nerve thus indicating that transection of the distal portion of the nerve produces marked changes throughout the length of the injured axons.

Published
1971

28. Postnatal differentiation of the granule cells in the hippocampus and cerebellum: a histochemical study

Author

Georg W. Kreutzberg and Gopal D. Das

Subjects
Cerebellum, Histology, biology, Histocytochemistry, Granule (cell biology), Acid Phosphatase, Cell Differentiation, Cell Biology, Granule cell, Hippocampus, Enzyme assay, Cell biology, Succinate Dehydrogenase, Medical Laboratory Technology, medicine.anatomical_structure, biology.protein, medicine, Neuropil, Animals, Fascia dentata, Rabbits, Oxidoreductases, Molecular Biology, Developmental biology
Abstract

Postnatal differentiation of the granule cells in the fascia dentata and the granule cell layer of cerebellum is described in terms of enzymatic activity with NADPH-R, NADH-R, SDH and Ac. Ph. In relation to this the neuropil, where the granule cells have their dendritic ramifications, has been studied. The granule cells in both the regions, after a progression of enzymatic changes, achieve an asymptotic level of enzyme activity around 16 days postnatally.

Published
1967

29. Enzyme histochemistry of human brain tumors and their tissue cultures with special reference to the oxidoreductases in the glioblastoma multiforme

Author

Filippo Gullotta, Margarete Minauf, and Georg W. Kreutzberg

Subjects
Histology, Reductase, Biology, Tissue culture, Fresh Tissue, Culture Techniques, medicine, Cytochrome c oxidase, Humans, Molecular Biology, chemistry.chemical_classification, Brain Neoplasms, Histocytochemistry, Enzyme histochemistry, Brain, Cell Biology, Human brain, medicine.disease, Molecular biology, Medical Laboratory Technology, medicine.anatomical_structure, Enzyme, chemistry, Biochemistry, biology.protein, Glioblastoma, Oxidoreductases
Abstract

Fresh tissue and tissue cultures of 80 glioblastoma multiforme and 12 monstrocellular sarcoma were histochemically investigated. The activity of the following enzymes was demonstrated in the biopsies and tissue cultures of every tumor: NADH-tetrazolium reductase, NADPH-tetrazolium reductase, lactic dehydrogenase, succinic dehydrogenase, glutamic acid dehydrogenase and cytochrome oxidase. No major differences in the relative activity pattern was shown when fresh tissue and tissue cultures were compared, nor did the enzymatic pattern change during the four week observation time.

Published
1966

31. Neuronal dynamics and axonal flow. IV. Blockage of intra-axonal enzyme transport by colchicine

Author

Georg W. Kreutzberg

Subjects
Male, Cytoplasm, Nerve fiber, Biology, Axonal Transport, chemistry.chemical_compound, Microtubule, medicine, Colchicine, Animals, Axon, Dihydrolipoamide Dehydrogenase, Neurons, Multidisciplinary, Histocytochemistry, Biological Transport, NAD, Acetylcholinesterase, Sciatic Nerve, Axons, Rats, Biological Sciences: Physiology, medicine.anatomical_structure, chemistry, Biochemistry, Axoplasmic transport, Biophysics, Neuron, Sciatic nerve
Abstract

The fact that the nucleated center of the nerve cell is the major source of the macromolecular materials required in the maintenance and function of the whole neuron requires the operation of a steady cellulifugal convection of these supplies into and down the nerve fiber. This proximo-distal traffic has been firmly established, but the mechanisms involved in it are still poorly understood. Besides the slow ( ca . 1 mm per day) advance of the axonal column as a whole (“axonal flow” in the strict sense), the demonstration of additional, much faster, traffic rates (up to several cm per day) calls for special conduits within the axon (“intra-axonal flow”). To test the possible role of neurotubules (average width:220 Å) in this traffic, the drug colchicine, known for its immobilizing effect on microtubules in other types of cells, was locally injected into peripheral nerves. This resulted in a major blockage of the proximo-distal movement of a test enzyme, acetylcholinesterase, into and through the injected zone, the extent of blockage varying with the applied dosage. By analogy, the neurotubules thus seem to be definitely implicated in the motile mechanism of intra-axonal transport. By contrast, the movement of a mitochondrion-associated marker enzyme, diphosphopyridine nucleotide diaphorase, was not perceptibly affected (in the submaximal dosage range), which seems to signify that the proximo-distal shift of mitochondria, for which the slow axonal flow acts as carrier, has gone on uninterruptedly. The experiments thus indicate the possibility of uncoupling the axonal and intra-axonal transport mechanisms.

Published
1969

32. Discussion

Author

Gopal D. Das and Georg W. Kreutzberg

Published
1968

33. Changes of Coenzyme (TPN) Diaphorase and TPN-linked Dehydrogenase during Axonal Reaction of the Nerve Cell

Author

Georg W. Kreutzberg

Subjects
Neurons, Multidisciplinary, biology, Histocytochemistry, Chemistry, Cell, Coenzymes, Dehydrogenase, Axonal Reaction, Axons, Cofactor, TPN Diaphorase, medicine.anatomical_structure, nervous system, Biochemistry, Diaphorase, Retrograde Degeneration, medicine, biology.protein, Humans, sense organs, Oxidoreductases, skin and connective tissue diseases
Abstract

Changes of Coenzyme (TPN) Diaphorase and TPN-linked Dehydrogenase during Axonal Reaction of the Nerve Cell

Published
1963

34. Dendritic secretion: A way for the neuron to communicate with the vasculature

Author

L. Tóth and Georg W. Kreutzberg

Subjects
Motor Neurons, Basement membrane, Vasa Vasorum, Guinea Pigs, Dendrites, General Medicine, Acetylcholinesterase, Basement Membrane, Rats, Cell biology, Microscopy, Electron, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Vasa vasorum, medicine, Animals, Secretion, Neuron, Ecology, Evolution, Behavior and Systematics
Published
1974

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