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In Bohr's method1, 2 for calculating the oxygen physically dissolved in blood or hemoglobin solution the assumption is made that the solubility of the gas in these fluids is as it is in water. In actual practice nitrogen is determined in blood or hemoglobin solution and from this and the solubility of oxygen in water the amount of oxygen physically dissolved in blood or hemoglobin solution is calculated. Direct determination of the oxygen in suspensions of cells or solutions of hemoglobin which have previously been treated with sodium nitrite, after which treatment it is supposed3 hemoglobin no longer combines with oxygen, shows that the oxygen is greater than is accounted for in Bohr's method of calculation. Assuming the same physical properties for hemoglobin after treatment with nitrite it would appear that a correction should be made in the usual determinations of oxygen chemically combined with hemoglobin.Treatment of cells or hemoglobin. (1) Cells: A mixture of 2 volumes of 0.9% sodium chloride solu...

It was reported1 that the total base content of the abdominal muscle of rabbits receiving an injection of 25% NaCl in the pleural cavity was considerably higher than that of control animals receiving the same quantity of normal saline. The permeability of the muscle to the sodium ion having been thus demonstrated, the total base content of muscle might be expected to increase with alkalosis and to decrease with acidosis. The experiments reported in this paper are designed to test this point. Some further experiments with NaCl injection are included.Rabbits were anesthetized with morphine hydrochloride (20 mg. per kg.). The abdominal wall was slit open, the ureters were tied, and the abdominal wall closed. Acidosis or alkalosis was produced by giving HCl, NaHCO3 or Na2CO3 solution. In some experiments the solution was introduced into the stomach by means of a tube, in others it was injected into the femoral vein at a rate of 1 cc. per minute. One-half to 2 hours after the introduction of the solution, bloo...

For the determination of CO2 in muscle and other tissues it is obviously necessary to modify the gas burette of Van Slyke and Neill1 so that relatively large pieces of material can be introduced into the burette. Ferguson and Irving2 have proposed to divide the burette into 2 separate parts just below the 2 cc. mark, the 2 parts being connected by means of a ground joint and held together with a pair of springs. In our laboratory several modifications have also been used. The one that seems most satisfactory is shown in Fig. 1.The new feature of the burette is the enlargement of the diameter of the lower stem of the burette and the introduction of a 15 cc. bulb between the burette and the flexible point. The short stem of the bulb is ground on the outside to fit the lower stem of the burette which is ground on the inside. The joint is surrounded by a cup for mercury seal and the cup is provided with an annular rubber cover to prevent the escape of mercury during shaking.For the determination of CO2 about ...