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Your search keyword '"Feng Wu"' showing total 2 results
Start Over Author "Feng Wu" Publication Year Range More than 50 years ago
2 results on '"Feng Wu"'

1. Zhongguo de zhi wu you

Author

Feng, Wu, Zhang, Lude, Institute of Botany, Chinese Academy of Sciences, Feng, Wu, and Zhang, Lude

Subjects
Oils and fats
Published
1953

2. Total Synthesis of Acetate from CO 2 : Methyltetrahydrofolate, an Intermediate, and a Procedure for Separation of the Folates

Author

Tsai-Feng Wu, Donald J. Parker, and Harland G. Wood

Subjects
Swine, Physiology and Metabolism, Acetates, Kidney, Microbiology, Cell-free system, Electron Transport, chemistry.chemical_compound, Hydrolysis, Folic Acid, Methionine, Clostridium, Glutamates, Enterococcus faecalis, Methods, Animals, Homocysteine, Molecular Biology, chemistry.chemical_classification, Carbon Isotopes, Chromatography, Cell-Free System, biology, Total synthesis, Methyltransferases, Carbon Dioxide, Chromatography, Ion Exchange, biology.organism_classification, Clostridium thermoaceticum, Culture Media, Enzymes, Linear gradient, Freeze Drying, Enzyme, chemistry, Biochemistry, Biological Assay
Abstract

Whole-cell preparations of Clostridium thermoaceticum were exposed to a short pulse of 14 CO 2 under conditions in which double-labeled acetate was synthesized. Radioactive methyltetrahydrofolate monoglutamate, diglutamate, and triglutamates were isolated from extracts of the cells. The radioactivity was found to be exclusively in the five methyl position. The specific activities of the methyltetrahydrofolate derivatives were very high and were in accord with the proposal that methyltetrahydrofolates are the precursors of the methyl of acetate. A new method of separation of folates employing QAE-Sephadex chromatography and a linear gradient with triethylammonium bicarbonate is presented which completely resolves the common folate monoglutamates and, upon freeze-drying, yields salt-free preparations.

Published
1971

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