1. THE USE OF MUTANT MYELOMA CELLS TO EXPLORE THE PRODUCTION OF IMMUNOGLOBULINS
- Author
-
L. Frank, T. Kelly, S. Weitzman, David H. Margulies, Barbara K. Birshtein, J.-L. Preud'homme, S. L. Morrison, B. Dharmgrongartama, Matthew D. Scharff, and W. M. Kuehl
- Subjects
High rate ,chemistry.chemical_compound ,Glycosylation ,chemistry ,biology ,Mutant ,biology.protein ,Mutagenesis (molecular biology technique) ,Secretion ,Antibody ,Primary sequence ,Molecular biology - Abstract
A method has been developed for identifying and quantifying mouse myeloma cells which have undergone mutations in immunoglobulin production. Such variants arise at a very high rate both spontaneously and with mutagenesis. Mutagenesis results in primary sequence variants which are blocked in a variety of steps in the synthesis, assembly, glycosylation, and secretion of H chains. These mutants can be used to investigate the production and genetic control of immunoglobulins.
- Published
- 1975
- Full Text
- View/download PDF