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7. El nucleolo : Componentes bioquímicos. Estudio citológico y bioquímico en algunos vegetales (Allium cepa, Vicia faba, Zea mays, Nitella clavata, Vaucheria sp., Acetabularia sp.) y animales (Drosophila virilis, Smittia sp., Bufo arenarum, Gallus gallus, Cavia porcellus)

Author

Tandler, Carlos Juan and De Carlo, J. M.

Abstract

Una revisión bibliográfica acerca del nucleolo revela que pese a los numerosos trabajos citológicos y bioquímicos del que fuera objeto, su función precisa dentro de la célula es desconocida. En esta tesis se han descripto hallazgos que representan un nuevo aporte a la bioquímica y fisiología del nucleolo, y comprenden: (A) La presencia universal de una sustancia de alto peso molecular localizada específicamente en los nucleolos de las células vegetales y animales, aun no identificada químicamente y caracterizada por reducir fuertemente las sales de plata y emulsiones fotográficas. No está presente en los cromosomas y área nuclear cuando desaparece el nucleolo durante la división celular. En metafase parece localizarse en el citoplasma. (B) Origen del nucleolo: utilizando la técnica anterior se demostró que durante la ana-telofase la sustancia nuclear se origina en contacto con todos los cromosomas, la que por fusión progresiva da lugar a la formación de pequeños cuerpos "prenucleolares". Estos a su vez se organizan finalmente en el nucleolo definitivo. El papel del organizador nucleolar consiste en la colección del material nucleolar disperso en el núcleo. También el microscopio electrónico está suministrando evidencia de la existencia de los cuerpos prenucleolares en diversos materiales biológicos. (C) La existencia de un pool de sustancias de bajo peso molecular-en su mayor parte o en su totalidad constituído por iones ortofosfato- y demostrado por una nueva técnica citoquímica. No está presente en los cromosomas y área nuclear durante la mitosis. La localización obtenida corresponde a una distribución real de estos iones en el nucleolo y no a un artificio de la técnica. La evidencia sugiere tambien algún tipo de combinación física, estructural, con el denso material nucleolar. A juzgar por las imágenes dadas por el microscopio electrónico del tejido fijado en acetato de plomo, la distribución de los iones ortofosfato sería el precursor del ácido ribonucleico (RNA) nucleolar. (D) Se demostró por medio de la autoradiografía de alta resolución que el fosfato radioactivo (P^32O4) no es un buen precursor del RNA nucleolar en células en división mitótica, en contraposición a lo observado con células en crecimiento contínuo (glándulas salivales de Dipteros). Siendo el nucleolo uno de los lugares mas activos en el metabolismo del RNA, evidenciado por numerosos trabajos usando ribonucleósidos radioactivos, la explicación del fenómeno observado radicaría en la existencia del pool de ortofosfato nucleolar. En efecto, ese pool es relativamente impermeable al p^32. Si este pool es usado en el nucleolo para la síntesis y degradación del RNA nucleolar, este RNA tendrá una baja actividad específica relativa como RNA-P^32, que es la que realmente ocurre. Este hallazgo incide en las hipótesis formuladas por algunos investigadores acerca de la probable función del RNA nucleolar o indica que es imposible una contribución masiva del RNA cromosomal al nucleolo, así como también que el nucleolo suministre todo el RNA ribosomal al citoplasma. (E) Se logró demostrar en los nucleolos de glándulas salivares de Dípteros la presencia de la enzima RNA-metilasa. En este trabajo se describe el empleo de la metionina marcada en el grupo metilo (-C^14H3) como precursora de un RNA, empleando la técnica autoradiográfica al nivel intracelular. La transferencia del grupo metilo de la metionina al RNA nucleolar no es afectada por la puremicina, antibiótico que inhibe la incorporación de la metionina en las proteínas. La radioactividad aparece en las bases metiladas del RNA nucleolar tal como indica la separación cromatográfica del hidrolizado alcalino de las glándulas salivares. La metilación observada representa muy probablemente un aspecto de la síntesis de transfer-RNA ya que esta especie de RNA es la única que posee bases metiladas. (F) Las glándulas salivares del género Smittia (Díptera) constituyen un material excelente para el análisis de la relación entre el organizador nucleolar y el RNA nucleolar por la disposición espacial de ambas estructuras. Se observa que el RNA nucleolar no proviene en especial del organizador. La función del organizador reside en la colección del material nucleolar, la que está de acuerdo con el modo de formación del nucleolo (vease punto B). Fil: Tandler, Carlos Juan. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Published
1963

8. Alegación del derecho que assiste a Don Luis Juan de Torres y Zanoguera, Curador de Don Carlos Juan de Torres, Conde de Peñalva su hermano. En la causa que en dicho nombre sigue contra el Administrador de la Administracion dexada por Doña Mariana Zanoguera. Sobre el dominio util de las carnicerias de Pescadores de esta Ciudad

Author

Peñalba, Luis Juan de Torres y Zanoguera y Mingot de Rocafull, Conde de, Peñalba, Carlos Juan de Torres y Zanoguera y Mingot de Rocafull, Conde de, Zanoguera, Mariana, and Nebot y Sans, José (m.1754), altres

Subjects
Judicis Herències i successions Comunitat valenciana S.XVIII Obres anteriors a 1800
Abstract

La segona sequència de 26 pàg. correspon a: "Adicion a la Alegacion en derecho, que se formò por Don Luis Juan de Torres y Zanoguera ... sobre el dominio util de las Carnicerias de Pescadores de esta Ciudad, que por discordia ha passado à la otra Sala" Port. orlada Grav. calc. de "S. Juan Bautista" a la port. Caplletres ornades i sobre el tít. de l'Adició [cristus Sign.: [A]2, B-I2, K3 ; A-F2, G1 El f. pleg. és un arbre genealògic L'Adició sense port. però amb paginació i signatures tip. pròpies Notes al marge i envoltant el text en: "Alegacion del derecho..." Reclams

Published
1743

9. Discurso iuridico por el ilustre don Ioseph Vicente Belvis de Moncada, Marques de Belgida, &c. Contra la egrecia doña Iuana Emanuela Mingot, Condesa viuda de Peñalva, y contra Luis Iuan Torres, tambien su hijo segundogenito. En el pleito petitorio que p

Author

Belgida, José Vicente Belvis y Moncada, Marqués de, Peñalva, Juana Manuela Mingot, Condesa de, Peñalva, Carlos Juan Torres, Conde de, Torres, Luis Juan, and Zanoguera Fenollet y de Blanes, Mariana

Abstract

A 110/112(17)

Published
1725

11. Alegacion en hecho, y derecho, por don Ioseph Vicente Beluis y Moncada, Marques de Belgida, & c. Con doña Iuana Manuela Mingot, Condesa viuda de Peñalva, madre, y curadora de don Carlos Iuan Torres, Conde de Peñalva; y con don Luis Iuan Torres, tambien

Author

Belgida, José Vicente Belvis y Moncada, Marqués de, Ferrer, Juan Bautista, Peñalva, Juana Manuela Mingot, Condesa de, Peñalva, Carlos Juan Torres, Conde de, Torres, Luis Juan, and Zanoguera Fenollet y de Blanes, Mariana

Abstract

A 110/112(18)

Published
1729

12. Letter from Carlos Finley to William C. Gorgas, 1905-04-28

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a grant from Institute of Museum and Library Services (IMLS).

Published
1905

13. Letter from Carlos Finley to William C. Gorgas, 1904-02-25

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a grant from Institute of Museum and Library Services (IMLS).

Published
1904

14. Letter from William C. Gorgas to Dr. Charles Finlay, 1906-05-11

Author

Finlay, Carlos Juan, 1833-1915 (Addressee), Gorgas, William Crawford, 1854-1920 (Correspondent), Finlay, Carlos Juan, 1833-1915 (Addressee), and Gorgas, William Crawford, 1854-1920 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a grant from Institute of Museum and Library Services (IMLS).

Published
1906

15. Letter from Carlos Finley to William C. Gorgas, 1903-12-16

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a grant from Institute of Museum and Library Services (IMLS).

Published
1903

16. Letter from W. C. Gorgas, Chief Sanitary Officer, Ancon, Panama, to Dr. Carlos Finlay, Chief Sanitary Officer, Havana, Cuba, February 20, 1906

Author

Finlay, Carlos Juan, 1833-1915 (Addressee), Gorgas, William Crawford, 1854-1920 (Correspondent), Finlay, Carlos Juan, 1833-1915 (Addressee), and Gorgas, William Crawford, 1854-1920 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a gift from EBSCO Industries.

Published
1906

17. Letter from W. C. Gorgas to Dr. Carlos Finlay, Chief Sanitary Officer, Havana, Cuba, June 16, 1906

Author

Finlay, Carlos Juan, 1833-1915 (Addressee), Gorgas, William Crawford, 1854-1920 (Correspondent), Finlay, Carlos Juan, 1833-1915 (Addressee), and Gorgas, William Crawford, 1854-1920 (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a gift from EBSCO Industries.

Published
1906

18. Letter from Carlos J. Finlay, Havana, Cuba, to Colonel Gorgas, January 30, 1906

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

Gift of Aileen Gorgas Wrightson, 1947 and 1952; Thomas W. Martin, 1952; Jessie Palfrey Leake, Minna Palfrey Tait, Aileen Gorgas Wrightson, Gene Palfrey Ellis, and William Gorgas Palfrey, 1958; Gorgas Memorial Institute of Tropical Medicine and Hygiene and National Library of Medicine, 1991; and Dr. William G. Palfrey and Gene Marie Palfrey Ellis, 1993

Published
1906

19. Letter from Carlos J. Finlay, Havana, Cuba, to Colonel Gorgas, Ancon, Panama, November 10, 1906

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

Gift of Aileen Gorgas Wrightson, 1947 and 1952; Thomas W. Martin, 1952; Jessie Palfrey Leake, Minna Palfrey Tait, Aileen Gorgas Wrightson, Gene Palfrey Ellis, and William Gorgas Palfrey, 1958; Gorgas Memorial Institute of Tropical Medicine and Hygiene and National Library of Medicine, 1991; and Dr. William G. Palfrey and Gene Marie Palfrey Ellis, 1993

Published
1906

20. Letter from Carlos J. Finlay, Havana, Cuba, to Colonel Gorgas, Ancon, Panama, August 30, 1906

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

Gift of Aileen Gorgas Wrightson, 1947 and 1952; Thomas W. Martin, 1952; Jessie Palfrey Leake, Minna Palfrey Tait, Aileen Gorgas Wrightson, Gene Palfrey Ellis, and William Gorgas Palfrey, 1958; Gorgas Memorial Institute of Tropical Medicine and Hygiene and National Library of Medicine, 1991; and Dr. William G. Palfrey and Gene Marie Palfrey Ellis, 1993

Published
1906

21. Letter from Carlos J. Finlay, Havana, Cuba, to Colonel Gorgas, August 1908

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), and Finlay, Carlos Juan, 1833-1915 (Correspondent)

Abstract

Gift of Aileen Gorgas Wrightson, 1947 and 1952; Thomas W. Martin, 1952; Jessie Palfrey Leake, Minna Palfrey Tait, Aileen Gorgas Wrightson, Gene Palfrey Ellis, and William Gorgas Palfrey, 1958; Gorgas Memorial Institute of Tropical Medicine and Hygiene and National Library of Medicine, 1991; and Dr. William G. Palfrey and Gene Marie Palfrey Ellis, 1993

Published
1908

22. Wedding invitation from Carlos J. and Adela Finlay, Havana, Cuba, to Mr. and Mrs. W. C. Gorgas, July 1,1903

Author

Gorgas, William Crawford, 1854-1920 (Addressee), Gorgas, Mary, 1857-1944 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), Finlay, Adele (Correspondent), Gorgas, William Crawford, 1854-1920 (Addressee), Gorgas, Mary, 1857-1944 (Addressee), Finlay, Carlos Juan, 1833-1915 (Correspondent), and Finlay, Adele (Correspondent)

Abstract

This is an item from the William Crawford Gorgas papers. This collection includes material created by and written about Gorgas, as well as material created by other Gorgas family members. His diaries and journals illuminate his life and work for the U.S. Army as a surgeon and span the years he worked in Cuba and Panama. The collection includes official reports and other documents Gorgas wrote and collected, as well as articles and other publications written about Gorgas and his work in sanitation and disease prevention, particularly yellow fever. Correspondence, articles, and other items document the numerous awards and tributes Gorgas received during his life and memorials after his death in 1920. In addition to William Crawford Gorgas material, the collection includes other material belonging to Gorgas family members including Marie Gorgas and their daughter, Aileen Gorgas Wrightson. In 1924, his widow Marie Gorgas published William Crawford Gorgas: His Life and Work. This collection includes manuscripts, galley proofs, and published versions of her work., The digitization of this collection was funded by a gift from EBSCO Industries.

Published
1903

23. El primer amor del mundo

Author

Sheen, Fulton J., 1895-1979, Vega, Carlos Juan, Sheen, Fulton J., 1895-1979, and Vega, Carlos Juan

Published
1954

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