1. Studies on a hemolytic factor of cobra venom requiring a heat-labile serum factor
- Author
-
Gerald B. Phillips
- Subjects
Electrophoresis ,Paper ,Erythrocytes ,Hot Temperature ,Guinea Pigs ,Biophysics ,Phospholipid ,Biology ,Biochemistry ,Hemolysis ,Guinea pig ,chemistry.chemical_compound ,Immune system ,medicine ,Animals ,Molecular Biology ,Phospholipase A ,Sheep ,Heparin ,Venoms ,Phosphatidylethanolamines ,Snakes ,Complement System Proteins ,Haemolysis ,medicine.disease ,Blood ,chemistry ,Lytic cycle ,Phospholipases ,lipids (amino acids, peptides, and proteins) ,Rabbits ,Dialysis ,medicine.drug - Abstract
Cobra venom caused the hemolysis of unsensitized sheep erythrocytes in the presence of guinea pig serum. Prior heating of the serum at 56° for 30 min of addition of hepatin prevented hemolysis. Heparin also inhibited immune hemolysis. The hemolytic activity of cobra venom could not be attributed to its phospholipase A or “direct lytic factor” content. Phospholipid analysis suggested that the hemolysis produced by the hemolytic factor was not the result of erythrocyte phospholipid breakdown. Hydrolysis of almost all of the erythrocyte phosphatidyl ethanolaine and phosphatidyl serine to their “lyso” derivatives could be produced without causing hemolysis. Hemolytic factor was inactivated by heating at 100° for 15 min and was not dialyzable. Hemolytic factor hemolysis appears to require complement.
- Published
- 1970