1. The Nucleotide Sequence of N-Formyl-Methionyl-Transfer RNA.
- Author
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Dube, S. K., Marcker, K. A., Clark, B. F. C., and Cory, S.
- Subjects
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NUCLEOTIDE sequence , *TRANSFER RNA , *ESCHERICHIA coli , *CHEMICAL purification , *CHROMATOGRAPHIC analysis , *DIETHYLAMINOETHANOL , *SEPHADEX , *CELLULOSE , *METHIONYL transfer RNA - Abstract
32P-labelled N-formyl methionyl transfer RNA from Escherichia coli strain CA265 was purified by chromatography on a column DEAE Sephadex followed by chromatography on benzoylated DEAE cellulose. Nucleotides from ribonuclease T1 and pancreatic ribonuclease digests of tRNAF were separated and their sequences determined. tRNAF consists of 77 nucleotides of which pCG is 5′-terminal and CAACCOH is 3′-terminal. [ABSTRACT FROM AUTHOR]
- Published
- 1969
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