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Your search keyword '"Zhao, Xiaohui"' showing total 3,418 results
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3,418 results on '"Zhao, Xiaohui"'

8. NLRs derepress MED10b- and MED7-mediated repression of jasmonate-dependent transcription to activate immunity.

Author

Wu, Qian, Tong, Cong, Chen, Zhengqiang, Huang, Shen, Zhao, Xiaohui, Hong, Hao, Li, Jia, Feng, Mingfeng, Wang, Huiyuan, Xu, Min, Yan, Yuling, Cui, Hongmin, Shen, Danyu, Ai, Gan, Xu, Yi, Li, Junming, Zhang, Hui, Huang, Changjun, Zhang, Zhongkai, Dong, Suomeng, Wang, Xuan, Zhu, Min, Tao, Xiaorong, and Dinesh-Kumar, Savithramma

Subjects
Mediator complex, NLRs, Sw-5b, effector-triggered immunity, Plant Immunity, Cyclopentanes, Transcription Factors, Mediator Complex, Arabidopsis Proteins
Abstract

Plant intracellular nucleotide-binding domain, leucine-rich repeat-containing receptors (NLRs) activate a robust immune response upon detection of pathogen effectors. How NLRs induce downstream immune defense genes remains poorly understood. The Mediator complex plays a central role in transducing signals from gene-specific transcription factors to the transcription machinery for gene transcription/activation. In this study, we demonstrate that MED10b and MED7 of the Mediator complex mediate jasmonate-dependent transcription repression, and coiled-coil NLRs (CNLs) in Solanaceae modulate MED10b/MED7 to activate immunity. Using the tomato CNL Sw-5b, which confers resistance to tospovirus, as a model, we found that the CC domain of Sw-5b directly interacts with MED10b. Knockout/down of MED10b and other subunits including MED7 of the middle module of Mediator activates plant defense against tospovirus. MED10b was found to directly interact with MED7, and MED7 directly interacts with JAZ proteins, which function as transcriptional repressors of jasmonic acid (JA) signaling. MED10b-MED7-JAZ together can strongly repress the expression of JA-responsive genes. The activated Sw-5b CC interferes with the interaction between MED10b and MED7, leading to the activation of JA-dependent defense signaling against tospovirus. Furthermore, we found that CC domains of various other CNLs including helper NLR NRCs from Solanaceae modulate MED10b/MED7 to activate defense against different pathogens. Together, our findings reveal that MED10b/MED7 serve as a previously unknown repressor of jasmonate-dependent transcription repression and are modulated by diverse CNLs in Solanaceae to activate the JA-specific defense pathways.

Published
2023

9. Detection of Anomalies and Explanation in Cybersecurity

Author

Samariya, Durgesh, Ma, Jiangang, Aryal, Sunil, Zhao, Xiaohui, Filipe, Joaquim, Editorial Board Member, Ghosh, Ashish, Editorial Board Member, Prates, Raquel Oliveira, Editorial Board Member, Zhou, Lizhu, Editorial Board Member, Luo, Biao, editor, Cheng, Long, editor, Wu, Zheng-Guang, editor, Li, Hongyi, editor, and Li, Chaojie, editor

Published
2024
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46. EFFECT OF miR-429 ON THE PROLIFERATION AND MIGRATION OF BREAST CANCER CELLS AND ITS MECHANISM

Author

NIU Tingting, WU Li, HOU Lin, QIU Biru, ZHAO Xiaohui, LI Jinyang

Subjects
breast neoplasms, micrornas, eukaryotic initiation factor-4e, cell proliferation, cell movement, Medicine
Abstract

Objective To investigate the effect of miR-429 on the proliferation and migration of breast cancer cells and its mechanism. Methods RT-qPCR and Western blotting were used to measure the relative mRNA and protein expression levels of eIF4E in breast cancer MCF-7, MDA-MB-231, MDA-MB-468, and BT-549 cells and normal breast epithelial MCF-10A cells. MCF-7 cells were transfected with miR-429 mimics (group A), mimics NC (group B), miR-429 inhibitors (group C), and inhibitors NC (group D), and RT-qPCR, CCK-8 assay, wound healing assay, and Western blotting were used to measure the expression of miR-429, cell proliferation and migration, and the relative mRNA and protein expression levels of eIF4E in groups A-D. The 293T cells were transfected with eIF4E-3'UTR-Wt+miR-429 mimics (group E), eIF4E-3'UTR-Wt+mimics NC (group F), eIF4E-3'UTR-Mut+miR-429 mimics (group G), and eIF4E-3'UTR-Mut+mimics NC (group H), and relative luciferase activity was measured for groups E-H. The targeted regulatory effect of miR-429 on eIF4E was analyzed. Results RT-qPCR and Western blotting showed that the relative mRNA and protein expression levels of eIF4E in MCF-7, MDA-MB-231, MDA-MB-468, and BT-549 cells were significantly higher than those in normal breast epithelial MCF-10A cells (F=74.414,1 981.243,P

Published
2023
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