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22nd Congress of the European-Veterinary-Society-for-Small-Animal-Reproduction (EVSSAR) -- JUN 28-29, 2019 -- Berlin, GERMANY WOS: 000473067900129 … European Vet Soc Small Anim Reprod, Royal Canin, IDEXX Labs, Fujifilm, Minitube, Virbac, Biomerieux, Dr Fritz, Karl Storz Endoskope, LABORr KLINISCHE DIAGNOSTIK GMBH & CO KG, HSB Blendivet, Ceva

Objective and Method: A new series of benzothiazole-piperazine derivatives was synthesized and a complete chemical characterization of the novel compounds was provided. In vitro cytotoxic activities were screened against colorectal (HCT-116), breast (MCF-7) and hepatocellular (Huh7) cancer cell lines by Sulforhodamine B assay. Result and Discussion: All compounds showed cytotoxic activity against hepatocellular (Huh7) and breast (MCF-7) cancer cell lines. Dihalo substituted benzylpiperazine derivatives (2a, 2e) had the highest cytotoxic activities in all the tested cell lines. In addition, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities of synthesized compounds were investigated by in vitro Ellman’s method. Compound 2j led to moderate and selective inhibition against AChE. Docking study was utilized to understand the binding mode of compound 2j in comparision with donepezil on AChE. The other tested compounds showed weak or no inhibition against AChE as promising anticancer agents. © 2017 Bentham Science Publishers. This Project is supported by The Scientific & Technological Research Council of Turkey (TUBTAKI). Project number: 114S115.

The aim of this study was to evaluate the plasma concentration of insulin like growth factor-2 (IGF-2) and its expression in tumor tissue of dogs with malignant mammary tumors. Fifteen dogs were used in the study. Eight dogs with mammary tumors constituted group 1, and 7 healthy dogs constituted group 2. Diestrus phase in dogs was determined with vaginal cytological examination. Metastasis was evaluated by ultrasonographic examination of abdomen and thorax radiography. Blood samples were collected from the dogs, plasma was separated and IGF-2 levels were measured with ELISA method. Tumors in group 1 were classified according to Clinical Grading System defined by World Health Organization and were removed by using complete unilateral mastectomy technique. The histological malignancy grading of tumors was made according tubule formation, nuclear pleomorphism, and number of mitotic cells by using Notthingham method. In histopathological examination, 4 carcinosarcoma, 1 intraductal papillary carcinoma, 1 carcinoma, 1 complex type carcinoma, and 1 mix type carcinoma were detected. In immunohistochemical investigation, IGF-2 expression was detected in mammary acinar epithelial cells, myoepithelial cells, and some vascular smooth muscle cells but not in ductal epithelial cells in tumor tissue. Compared to healthy dogs, the increase in plasma IGF-2 concentration in dogs with malignant mammary tumors was statistically significant (P

/0000-0002-0636-4214 WOS: 000268128600005 PubMed: 25903514 This randomized study was designed to establish the efficacy of platelet-rich plasma (PRP) gel and topical estradiol alone, or in combination, on healing of full-thickness wounds created on the trunk of rabbits. Fifteen New Zealand white female rabbits and 60 wounds were studied. Four 2.25-cm(2) full-thickness wounds were created using a template and treatments including a sterile saline solution that were assigned randomly to the wounds. Wounds were bandaged, dressed intermittently, and assessed by wound measurements and a collection, of samples at 7, 14, and 21 days to evaluate healing. Variables of interest were hydroxyproline concentration and scored gross and microscopic morphologic characteristics reflective of wound healing. PRP gel + estradiol-treated wounds completely healed faster than wounds given other treatments. Granulation tissue growth to the skin level was faster (P < 0.05) in the PRP gel + estradiol group than the other groups. Only the PRP gel + estradiol group had a significantly lower collagen level than the sterile saline solutions on day 7 (P < 0.05). The PRP gel + estradiol group and estradiol group led to an increase in angiogenesis on day 14 (P < 0.05). Complete epithelization was observed only in the PRP gel + estradiol group compared with the other groups on day 7. PRP gel + estradiol-treatment enhanced healing in full-thickness wounds by reducing the contraction rate with a trend toward accelerating both epithelial migration and the angiogenic response.

YILDIZ, Kader/0000-0001-5802-6156; /0000-0002-0636-4214 WOS: 000251604000001 An Anatolian wild sheep (Ovis orientalis anatolica) from a wild ruminant farm near Kirikkale, Turkey, was necropsied. Parasitic nodules were situated beneath the pleura of the caudodorsal lobes of the lungs. First stage larvae of Cystocaulus ocreatus were detected both in bronchial fluid and in lung paraffin sections. Clinical and parasitological examinations of the other animals (4 dwarf goats and 3 wild sheep reared on the same farm) were also performed. First stage larvae of C. ocreatus, and eggs of Nematodirus spp. and Strongyloides spp. were observed in coproscopic examination. Anthelmintic treatment, Rabenzole (R) (rafoxanide and thiabendazole, Topkim), was administered to the animals infected by parasites. At the end of the treatment the combination of rafoxanide and thiabendazole was found to be markedly effective for the treatment of lungworm infection and no parasitic eggs were detected in the faeces of any of the treated animals.

The present study was conducted to immunolocalize 3?-hydroxysteroid dehydrogenase (3?-HSD), an enzyme metabolizing pregnenolone to progesterone in the choroid plexus of the lateral ventricle in sheep, as well as to measure progesterone concentration in cerebrospinal fluid (CSF) and plasma using radioimmunoassay (RIA). Akkaraman breed rams (n = 16) and ewes (n = 16) were utilized in the study. 3?-HSD was immunolocalized in choroid epithelial cells of the choroid plexus with an apparent cytoplasmic immunoreactivity. Progesterone was detected in CSF with no significant differences between the ewes (0.76 ± 0.14 ng/mL) and rams (0.74 ± 0.13 ng/mL) (p > 0.05). However, the plasma progesterone concentration in the ewes (0.27 ± 0.04 ng/mL) was significantly higher than that of the rams (0.11 ± 0.02 ng/mL) (p < 0.001). Consequently, CSF in sheep contains progesterone in significant levels. As evidenced by 3?-HSD immunoreactivity, choroid epithelial cells may be a site of progesterone synthesis in sheep.

/0000-0002-0636-4214 WOS: 000227165300003 The present study investigated the immunolocalization of CD 14 and the bacterial antigen of Pasteurella multocida serotype A :3 in normal and pneumonic rabbit lungs. To induce pneumonia, P. multocida serotype A :3 (4 x 10(6) CFU/ml) was inoculated intra-tracheally to twelve New Zealand White rabbits (Oryctolagus cuniculus) at age of 8-10 weeks while four rabbits received physiologic saline with the same route. Nine infected rabbits died with clinical sings of the disease between the 3(rd) and 13(th) days post inoculation. On day 14, the remaining three infected and control rabbits were euthanatized. The histopathological evaluation of the lung samples collected from nine infected rabbits with clinical symptoms revealed severe cases of fibrinopurulent and necrotic pneumonia and fibrinopurulent pleuritis. The bacterial antigen was immunolocalized in macrophages and degenerating leukocytes around the necrotic tissue and in exudates occupying the lumens of bronchia, bronchioles, and alveoli. CD14 immunoreactivity was present in alveolar macrophages, neutrophiles, alveolar epithelial cell surfaces, and lumens of the capillaries of the pneumonic lungs. In three rabbits, inoculated but presenting no clinical symptoms and no histopathological lesions, the bacterial antigen was found in the alveolar wall; however, like in the controls, CD14 immunoreactivity was present only in the lumen of capillaries. Double labelling immunohistochemistry indicated that CD14 was closely associated with the bacterial antigen in inflamed regions of the lung. Results suggested that interaction of the bacterial antigens and CD14 may have a critical role in lung pathologies resulting from P. multocida serotype A :3 infection.

/0000-0002-0636-4214 WOS: 000223398200003 This study was focused on serum and cerebrospinal fluid (CSF) biochemical changes in experimental Listeria monocytogenes infection in rabbits. Total of 20 New Zealand White rabbits were used. 14 New Zealand White rabbits were inoculated with Listeria monocytogenes 1/2a (L.m. 1/2a) by intracerebral way and 6 rabbits served as controls. Ten hours after the inoculation of the bacteria the rabbits developed symptoms of meningitis, with stiffness of the neck and fever. The existence and the severity of the inflammatory reaction were controlled by microscopic observation. The presence of bacteria into the cytoplasm of neutrophils and macrophages localised in inflammatory areas was confirmed by immunohistochemical analysis using rabbit polyclonal antibodies. We have analysed in serum and cerebrospinal fluid (CSF) the concentrations of glucose, total protein, and the enzymatic activities of aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT) and lactate dehydrogenase (LDH). After intracerebral inoculation of bacteria the glucose concentrations in the serum and CSF decreased to 428.69 +/- 5.50 mg/l and 309.56 +/- 3.93 mg/l (mean +/- SE) respectively compared to control values of 975.33 +/- 84.34 mg/l and 689.22 +/- 65.13 mg/l (p less than or equal to 0.001). Total protein contents, the activities ofASAT, ALAT and LDH in the serum of infected animals increased to 98.92 +/- 1.97 g/l, 327.87 +/- 22.18 U/l, 292.39 +/- 4.66 U/l, 153.28 +/- 3.04 U/l respectively compared to 64.42 +/- 1.14 g/l, 67.91 +/- 7.79 U/l, 74.25 +/- 1.79 U/l, 49.35 +/- 1.20 U/l in control rabbits. In CSF, increases in total protein concentrations and in enzyme activities were also evidenced in inoculated rabbits. Moreover, serum and CSF LDH activities were correlated with brain injury (p < 0.05).

A series of novel 3-methyl-1-[(4-substitutedpiperazin-1-yl)methyl]-1H-indoles (3a-1) were synthesized and their cytotoxicities were analyzed against 3 different human cell lines, including liver (HUH7), breast (MCF7) and colon (HCT116). The Mannich reaction of 3-methylindole (1) with 4-substitutedpiperazines (2) and formaldehyde resulted to the 3-methyl-1-[(4-substitutedpiperazin-1-yl)methyl]-1H-indoles (3a-1) in 38-69% yields. The investigation of anticancer screening revealed that the tested compounds showed comparable activity to the reference drug 5-fluorouracil and compounds 3g, 3h, 3i and 3k, had lower 50% inhibition (IC50) concentration than reference drug. Moreover, the cytotoxic effect of the most potent compound 3h on HUH7 and MCF7 cells through apoptosis was visualized by Hoechst staining and compared with paclitaxel, which is a mitotic inhibitor acting on microtubules. The morphological features of apoptosis were observed as condensed and fragmented nuclei that are similar to paclitaxel. The Scientific & Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108S009] The chemistry part of this Work was supported by a grant from The Scientific & Technological Research Council of Turkey (TUBITAK) (Project No. 108S009).

Background: In this study, nine novel compounds, bearing N-[2-(4-substituted piperazine-1-yl)acetyl]-N’-[bis-(4-fluorophenyl)methyl]piperazine structures were synthesized. Methods: Their cytotoxic properties were evaluated in vitro by NCI-60 Sulforhodamine B (SRB) assay against human cancer cell lines: Huh7 (hepatocellular), MCF7 (breast) and HCT116 (colorectal). Results and Conclusion: According to the activity data, most of the compounds are more cytotoxic than 5-fluorouracil against Huh7 and HCT116 cancer cell lines.

Synthesis and cytotoxic activities of 32 benzhydrylpiperazine derivatives with carboxamide and thioamide moieties were reported. In vitro cytotoxic activities of compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. In general, 4-chlorobenzhydrylpiperazine derivatives were more cytotoxic than other compounds. In addition, thioamide derivatives (6a-g) have higher growth inhibition than their carboxamide analogs. © 2014 Informa UK Ltd. All rights reserved.

Castellani, Gastone c/0000-0003-4892-925X; /0000-0002-0636-4214 WOS: 000316957200003 PubMed: 23206266 Purpose: To investigate the effects of low level electromagnetic field (low level-EMF) exposure, as frequently encountered in daily life, on the normal rat cornea using histological and stereological method. Methods: Twenty-two adult male Wistar rats were randomly divided into two groups: Study group (n = 11) and control group (n = 11). Rats in the study group were exposed to 2.45 GHz microwave (MW) radiation (11.96 +/- 0.89 V/m), 0.25 W/kg specific absorption rate (SAR) for 2 hours each day for 21 days. The corneal thickness and the anterior epithelium corneal thickness were measured using two different methods. Results: Using the histological method, the mean corneal thicknesses in the control and study group were 278.9 +/- 54.5 mu m, and 272.4 +/- 85.6 mu m, respectively. There was no statistically significant difference between the groups (p > 0.05). The anterior corneal epithelium thickness was 28.1 +/- 4.9 mu m in the control group and 31.7 +/- 5.5 mu m in the study group. There were statistically differences between the groups with regard to the thickness of anterior epithelium (p < 0.05). In the measurement made by the stereological method, the percentage of the cornea occupied by anterior corneal epithelium was 15.94% in the control group and 17.9% in the study group. Despite the fact that there was a relation between increased anterior epithelial area (AEA) and radiation exposure, no statistically significant relationship in area fraction of each compartment was found between the control and study groups. Conclusions: Results of this preliminary study show that exposure to MW radiation might cause alterations in the rat cornea.

This study presents the synthesis of nineteen 1-(substitutedbenzoyl)-4- benzhydrylpiperazine and 1-[(substitutedphenyl)sulfonyl]-4-benzhydrylpiperazine derivatives. In vitro cytotoxic activities of the compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. Among the test compounds, benzamide derivatives had high cytotoxic activity whereas sulfonamide derivatives showed variable 50% growth inhibition (GI50). © Georg Thieme Verlag KG Stuttgart · New York.