18 results on '"Xudan Yang"'
Search Results
2. Metanephric stromal tumor in an adult with PDGFRA mutation: a case report
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Sanjun Guo, Huan Qian, Hong Zhu, Yue Yang, Xudan Yang, and Huajun Sun
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Metanephric stromal tumor ,Adult ,BRAF V600E mutation ,PDGFRA mutation ,Small diameter tumor ,Pathology ,RB1-214 - Abstract
Abstract Background Metanephric stromal tumors (MST) are rare benign renal tumors that mainly occur in infants and children. Approximately 72% of MST in children have the B-Raf proto-oncogene serine/threonine kinase (BRAF) V600E mutation. To date, only five cases of adult MSTs have been reported and no clear genetic alterations have been found. Case presentation We report a case of MST in a 45-year-old woman who complained of left lower back pain for a week, accompanied by hypertension (150/79 mmHg). Magnetic resonance imaging (MRI) showed an abnormally enhanced nodule (1.1 cm in the middle of the left kidney), which was histopathologically consistent with an MST. The BRAF V600E mutation was not detected in tumor cells using PCR and next-generation sequencing (NGS). However, a platelet-derived growth factor receptor alpha (PDGFRA) mutation was detected in this case using NGS. The patient showed no recurrence or metastasis nine months after partial nephrectomy, and her blood pressure was consistently normal. Conclusion This is the first report of alterations in PDGFRA in MSTs. This result advances our knowledge of genetic variations in adult MSTs, which may have different gene alterations from MSTs in children.
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- 2023
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3. Perspectives of PDE inhibitor on treating idiopathic pulmonary fibrosis
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Xudan Yang, Zhihao Xu, Songhua Hu, and Juan Shen
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pulmonary fibrosis ,cAMP ,cGMP ,PDE inhibitor ,anti-fibrosis ,senescence ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive interstitial lung disease (ILD) without an identifiable cause. If not treated after diagnosis, the average life expectancy is 3–5 years. Currently approved drugs for the treatment of IPF are Pirfenidone and Nintedanib, as antifibrotic drugs, which can reduce the decline rate of forced vital capacity (FVC) and reduce the risk of acute exacerbation of IPF. However these drugs can not relieve the symptoms associated with IPF, nor improve the overall survival rate of IPF patients. We need to develop new, safe and effective drugs to treat pulmonary fibrosis. Previous studies have shown that cyclic nucleotides participate in the pathway and play an essential role in the process of pulmonary fibrosis. Phosphodiesterase (PDEs) is involved in cyclic nucleotide metabolism, so PDE inhibitors are candidates for pulmonary fibrosis. This paper reviews the research progress of PDE inhibitors related to pulmonary fibrosis, so as to provide ideas for the development of anti-pulmonary fibrosis drugs.
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- 2023
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4. A rare case of fetus in fetu in the sacrococcygeal region: CT and MRI findings
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Tao Lu, Junmei Ma, and Xudan Yang
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Fetus in fetu ,CT ,MRI ,Case report ,Pediatrics ,RJ1-570 - Abstract
Abstract Background Fetus in fetu is a rare condition in which a malformed fetus is found in the body of a living twin. The retroperitoneum is the most common location of this condition. However, the sacrococcygeal region is a rare site of the disease. The presence of vertebral bodies and limbs differentiates FIF from teratoma. Imaging modalities are important for diagnosing FIF. Case presentation A 12-months old boy was hospitalized because of a mass in the sacrococcygeal region. CT showed a large, complex mass with bony structure resembling sacrococcygeal bone, hip bone and the femur in the sacrococcygeal region of the boy. The blood supply of the mass was from the aorta of the host. MRI revealed the mass was connected with the dilated sacral canal of the host, which resulted in tethered cord. A preoperative diagnosis of FIF was made and surgery was performed to remove the mass. Surgical removal and subsequent pathological examination revealed the anencephalic fetus had limb buds and a sacrum but no axial skeleton, which supported the diagnosis of FIF. Conclusions CT and MRI played important roles in diagnosing FIF based on the location of the lesion.
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- 2021
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5. Severe Hypoglycemia Caused by a Giant Borderline Phyllodes Tumor of the Breast: A Case Report and Literature Review
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Yaoxia Liu, Min Zhang, Xudan Yang, Zhen Fan, Yi Li, Tao Wang, and Ping Chen
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phyllodes tumor ,breast ,hypoglycemia ,non-islet cell tumor hypoglycemia ,insulin-like growth factor-2 ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
A case of hypoglycemic coma caused by a giant borderline phyllodes tumor of the breast has been described. The patient, a 63-year-old woman, was admitted with recurrent unconsciousness. She had a giant breast tumor with decreased blood glucose, insulin, and C-peptide. The patient’s hypoglycemia resolved rapidly after resection of the breast tumor. Pathological examination indicated a borderline phyllodes tumor of the breast, and immunohistochemistry suggested high expression of insulin-like growth factor-2 (IGF-2) in the tumor tissue. A literature review is also included to summarize the clinical characteristics of such patients and to serve as a unique resource for clinical diagnosis and treatment of similar cases.
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- 2022
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6. Agenesis of the dorsal pancreas presenting with diabetic ketoacidosis – a case report and literature review
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Tian Yang, Xudan Yang, Luping Wang, and Jun Mo
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Agenesis of the dorsal pancreas ,Diabetic ketoacidosis ,Diabetes mellitus ,C-peptide release test ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Abstract Background Agenesis of the dorsal pancreas (ADP) is clinically rare, and it is usually accompanied by abdominal pain. Various disorders of glucose metabolism associating with ADP have been reported, but there are only two studies reporting a correlation between ADP and DKA in English literature. Case presentation We present a case of a patient with ADP accompanied by abdominal pain and diabetic ketoacidosis as the initial clinical presentation. A 30-year-old man presented with a 3-month history of recurrent onset of persistent mild epigastric pain, which worsen when eating. Laboratory tests revealed metabolic acidosis, hyperglycemia, and ketonuria. Phase contrast CT and MRCP showed the absence of the body and tail of the pancreas, as well as the dorsal pancreatic duct. The C-peptide release test indicated β-cell dysfunction. A combination therapy of insulin, pancreatic enzyme supplements, and mosapride citrate were administrated and the pain gradually resolved. Conclusions As glucose metabolism disorders can vary across different individuals, we advise clinicians to consider the diagnosis of ADP for a patient who presents with a glucose metabolism disorder accompanied by abdominal pain, pancreatitis or steatorrhea.
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- 2019
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7. Asymptomatic Broncholithiasis and Aspergillus Infection: A Case Report and Literature Review
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Songhua Hu, Xudan Yang, Yaqin Huang, and Zhihao Xu
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Pharmacology ,Infectious Diseases ,Pharmacology (medical) - Published
- 2023
8. Imaging Findings of Hepatic Ewing’s Sarcoma on Computed Tomography and Gadobenate Dimeglumine-enhanced Magnetic Resonance Imaging: A Case Report and Literature Review
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Wenhao Yang, Chong Yang, Xingchao Liu, Xudan Yang, Wenjia Di, and Tao Lu
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Hepatology ,medicine.diagnostic_test ,business.industry ,Ewing's sarcoma ,Magnetic resonance imaging ,Computed tomography ,medicine.disease ,Mri image ,Primitive neuroectodermal tumor ,medicine ,Sarcoma ,Signal intensity ,business ,Nuclear medicine ,GADOBENATE DIMEGLUMINE - Abstract
Ewing’s sarcoma (ES) is a tumor that often occurs in the long bones and rarely arises from visceral organs primarily. Here, we report a case of primary hepatic ES, discuss its computed tomography (CT) and gadobenate dimeglumine-enhanced magnetic resonance (MRI) features. This is the first Chinese and fifth primary hepatic ES case reported, based on a literature review. Imaging examinations showed that the tumor was solid, with necrosis and hemorrhage. Contrast-enhanced images showed that the tumor was hypervascular and especially had heterogeneous signal intensity on hepatobiliary phase MRI images. Intratumoral vessels and vascular invasion were also present.
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- 2021
9. Debulking and three‐dimensional reconstruction of a rare case of right atrium‐ventricular angiosarcoma
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Xudan Yang, Shengshou Hu, Keli Huang, Jin Tan, Qin Jiang, and Wenhua Li
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Tricuspid valve ,business.industry ,030204 cardiovascular system & hematology ,Chest pain ,medicine.disease ,Debulking ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,030228 respiratory system ,Concomitant ,cardiovascular system ,medicine ,Surgery ,Angiosarcoma ,cardiovascular diseases ,Radiology ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Pathological ,Artery - Abstract
A 48-year-old man presented with chest pain and ischemic manifestations according to an electrocardiogram due to coronary artery compression from a cardiac mass and was admitted to the emergency room and underwent extensive debulking followed by right atrium and ventricular three-dimensional reconstruction with concomitant tricuspid valve remodeling. He recovered a normal sinus rhythm and was discharged from the hospital a week later with a diagnosis of cardiac malignant angiosarcoma according to the pathological examination. He survived and had a normal cardiac structure and function performance, but vertebral metastasis was suspected after more than 4 months of follow-up after the procedure.
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- 2019
10. Agenesis of the dorsal pancreas presenting with diabetic ketoacidosis – a case report and literature review
- Author
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Xudan Yang, Jun Mo, Tian Yang, and Luping Wang
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Adult ,Male ,medicine.medical_specialty ,Abdominal pain ,Diabetic ketoacidosis ,Endocrinology, Diabetes and Metabolism ,Case Report ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Gastroenterology ,Agenesis of the dorsal pancreas ,Congenital Abnormalities ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Glucose Metabolism Disorder ,Internal medicine ,C-peptide release test ,medicine ,Humans ,Pancreas ,lcsh:RC648-665 ,business.industry ,Metabolic acidosis ,General Medicine ,medicine.disease ,Prognosis ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Ketonuria ,Pancreatitis ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Background Agenesis of the dorsal pancreas (ADP) is clinically rare, and it is usually accompanied by abdominal pain. Various disorders of glucose metabolism associating with ADP have been reported, but there are only two studies reporting a correlation between ADP and DKA in English literature. Case presentation We present a case of a patient with ADP accompanied by abdominal pain and diabetic ketoacidosis as the initial clinical presentation. A 30-year-old man presented with a 3-month history of recurrent onset of persistent mild epigastric pain, which worsen when eating. Laboratory tests revealed metabolic acidosis, hyperglycemia, and ketonuria. Phase contrast CT and MRCP showed the absence of the body and tail of the pancreas, as well as the dorsal pancreatic duct. The C-peptide release test indicated β-cell dysfunction. A combination therapy of insulin, pancreatic enzyme supplements, and mosapride citrate were administrated and the pain gradually resolved. Conclusions As glucose metabolism disorders can vary across different individuals, we advise clinicians to consider the diagnosis of ADP for a patient who presents with a glucose metabolism disorder accompanied by abdominal pain, pancreatitis or steatorrhea.
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- 2019
11. A case report of drug-induced ischemic colitis accompanied by ulcerative colitis
- Author
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Xudan Yang, Guanyu Zhou, Fei Xiong, and Pu Wang
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Diarrhea ,Drug ,medicine.medical_specialty ,media_common.quotation_subject ,Plasma Cells ,Gastroenterology ,Ischemic colitis ,Young Adult ,Internal medicine ,Humans ,Medicine ,Lymphocytes ,media_common ,Phagocytes ,Hepatology ,business.industry ,medicine.disease ,Ulcerative colitis ,Abdominal Pain ,Colitis, Ulcerative ,Female ,Gastrointestinal Hemorrhage ,business ,Colitis, Ischemic ,Drugs, Chinese Herbal - Published
- 2021
12. miR-19a-3p downregulates tissue factor and functions as a potential therapeutic target for sepsis-induced disseminated intravascular coagulation
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Yanmei Wu, Maojun Li, Feng Xiao, Xiaoxue Na, Jing Liao, Qing Jing, Rong Zhang, Sifen Lu, Ling Wan, Xiaohong Bai, Hui Jia, Yulin Kang, Haichuan Wang, Ji-Yun Yang, and Xudan Yang
- Subjects
Lipopolysaccharides ,Male ,MAPK/ERK pathway ,Down-Regulation ,Biochemistry ,Peripheral blood mononuclear cell ,Umbilical vein ,Thromboplastin ,Rats, Sprague-Dawley ,Tissue factor ,In vivo ,Sepsis ,Human Umbilical Vein Endothelial Cells ,Animals ,Humans ,Protein kinase B ,Cells, Cultured ,Pharmacology ,Chemistry ,Kinase ,Infant, Newborn ,Disseminated Intravascular Coagulation ,Rats ,MicroRNAs ,Cancer research ,Phosphorylation ,Female - Abstract
Sepsis-induced disseminated intravascular coagulation (DIC) is a common life-threatening terminal-stage disease with high mortality. This study aimed to identify effective miRNAs as therapeutic targets for DIC. Bioinformatics and luciferase reporter gene analyses were performed to predict miR-19a-3p and validate that it targets tissue factor (TF). Quantitative real-time PCR was used to detect the expression of miR-19a-3p and TF, and TF procoagulant activity was determined using the chromogenic substrate method. Western blotting was used to detect the protein levels of TF, AKT serine/threonine kinase (AKT), extracellular regulated protein kinases (ERK), nuclear factor kappa B (NF-κB) P65, NFKB inhibitor alpha (IκB-a) and their phosphorylated counterparts in cell experiments. Furthermore, a rat model was established to explore the potential of miR-19a-3p in DIC treatment. As a result, a human clinical study revealed that miR-19a-3p was downregulated and that TF was upregulated in neonates with sepsis-induced DIC compared with those in the control group. The luciferase reporter assay showed that TF was a direct target of miR-19a-3p. Cell experiments verified that the mRNA and protein levels of TF, and the p-AKT/AKT, p-Erk/Erk, p-P65/P65, p-IκB-a/IκB-a ratios, and TF procoagulant activity were significantly decreased in lipopolysaccharide (LPS) -induced human peripheral blood mononuclear cells (PBMCs) and human umbilical vein endothelial cells (HUVECs) inhibited by overexpression of miR-19a-3p, and that miR-19a-3p regulating TF was dependent on the NF-kB and AKT pathways. In vivo, miR-19a-3p injection into DIC rats suppressed the mRNA expression of TF; more importantly, significant improvements in coagulation function indicators and in histopathologies of lung and kidney were observed. In conclusion, miR-19a-3p may suppress DIC by targeting TF and might be a potential therapeutic target in treating sepsis-induced DIC.
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- 2021
13. Insights into the effect of N-acetyl-L-cysteine-capped CdTe quantum dots on the structure and activity of human serum albumin by spectroscopic techniques
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Meng Li, Chunguang Liu, Songlin Han, Rutao Liu, Yingxue Liu, Haoyu Sun, Xuejie Tan, and Xudan Yang
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Stereochemistry ,Chemistry ,technology, industry, and agriculture ,Biophysics ,General Chemistry ,equipment and supplies ,Condensed Matter Physics ,Human serum albumin ,Biochemistry ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Fluorescence spectroscopy ,body regions ,Hydrophobic effect ,Blood serum ,Quantum dot ,embryonic structures ,medicine ,Protein secondary structure ,medicine.drug ,Cysteine - Abstract
Quantum dots (QDs) are a kind of nanostructured semiconductor crystals with the size range of 1–10 nm. Their unique photophysical properties and potential toxicity to human health have aroused wide concern of scientists and general public. However, the interaction mechanism of QDs on human serum albumin (HSA, the vital protein in human blood) from both structural and functional perspectives is rarely reported. In the present work, effects of N-acetyl- L -cysteine-capped CdTe quantum dots with fluorescence emission peak at 612 nm (QDs-612) on the conformation and function of HSA were investigated by spectroscopic methods, molecular docking study and esterase activity assay. The hydrophobic interaction between HSA and QDs-612 was spontaneous with the binding constants calculated to be 6.85×105 L mol−1 (298 K) and 8.89×105 L mol−1 (308 K). The binding of QDs-612 to HSA induced the static quenching of fluorescence and the changes of secondary structure and microenvironment of Tyr-411 residue, which resulted in serious decrease on the hydrolysis of substrate p-nitrophenylacetate in esterase activity assay of HSA. This work confirms the possibility on direct interaction of QDs-612 with HSA and obtains a possible mechanism of relationship between conformation and function of HSA.
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- 2015
14. Amine-Cross-Linked Lignin-Based Polymer: Modification, Characterization, and Flocculating Performance in Humic Acid Coagulation
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Ruihua Li, Qinyan Yue, Wuchang Song, Baoyu Gao, Ruibao Jia, Meng Li, Shenglei Sun, and Xudan Yang
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chemistry.chemical_classification ,Flocculation ,Renewable Energy, Sustainability and the Environment ,Chemistry ,General Chemical Engineering ,Cationic polymerization ,General Chemistry ,Polymer ,complex mixtures ,chemistry.chemical_compound ,Chemical engineering ,Zeta potential ,Environmental Chemistry ,Organic chemistry ,Coagulation (water treatment) ,Humic acid ,Lignin ,Solubility - Abstract
In this study, a lignin-based flocculant (LNF) was synthesized by grafting amine groups into alkali lignin containing papermaking sludge. Characterization of LNF, such as FTIR, zeta potential, cationic degree, viscosity, and molecular weight, showed that the product was a cationic polymer with high solubility. LNF was used with aluminum sulfate (AS) and polyaluminum chloride (PAC) in humic acid coagulation to demonstrate its efficiency. Coagulation behavior and floc properties of LNF+PAC and LNF+AS dual-coagulant were comparatively evaluated. Results showed that the coagulation aid effect of LNF was independent of aluminum species. Addition of LNF could enhance humic acid removal efficiency and floc properties including size, strength, and fractal dimension significantly. The effect of solution pH on coagulation processes was also studied. Dual-coagulants showed the same variation trend as that of aluminum-based coagulants but enhanced coagulation performance during the investigative pH range. Flocs forme...
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- 2015
15. Toxic effects of chrysoidine on human serum albumin: isothermal titration calorimetry and spectroscopic investigations
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Rutao Liu, Meng Li, Songlin Han, Haoyu Sun, Yingxue Liu, and Xudan Yang
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Chromatography ,Hydrogen bond ,Chemistry ,Biophysics ,Isothermal titration calorimetry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Human serum albumin ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,body regions ,Residue (chemistry) ,Protein structure ,Chemistry (miscellaneous) ,embryonic structures ,medicine ,Molecule ,0210 nano-technology ,Protein secondary structure ,medicine.drug - Abstract
Chrysoidine is widely used in industry as a type of azo dye, and is sometimes used illegally as a food additive despite its potential toxicity. Human serum albumin (HSA) is one of the most important proteins in blood plasma and possesses major physiological functions. In the present study, the conformational and functional effects of chrysoidine on HSA were investigated by isothermal titration calorimetry (ITC), multiple spectroscopic methods, a molecular docking study and an esterase activity assay. Based on the ITC results, the binding stoichiometry of chrysoidine to HSA was estimated to be 1.5:1, and was a spontaneous process via a single hydrogen bond. The binding of chrysoidine to HSA induced dynamic quenching in fluorescence, and changes in secondary structure and in the microenvironment of the Trp-214 residue. In addition, the hydrogen bond (1.80 A) formed between the chrysoidine molecule and the Gln-211 residue. The esterase activity of HSA decreased following the addition chrysoidine due to the change in protein structure. This study details the direct interaction between chrysoidine and HSA at the molecular level and the mechanism for toxicity as a result of the functional changes induced by HSA structural variation upon binding to chrysoidine in vitro. This study provides useful information towards detailing the transportation mechanism and toxicity of chrysoidine in vivo. Copyright © 2015 John Wiley & Sons, Ltd.
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- 2015
16. Toxic effects of chrysoidine on human serum albumin: isothermal titration calorimetry and spectroscopic investigations
- Author
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Haoyu, Sun, Yingxue, Liu, Meng, Li, Songlin, Han, Xudan, Yang, and Rutao, Liu
- Subjects
Molecular Docking Simulation ,Spectrometry, Fluorescence ,Molecular Structure ,Protein Conformation ,p-Aminoazobenzene ,Esterases ,Humans ,Hydrogen Bonding ,Spectrophotometry, Ultraviolet ,Calorimetry ,Fluorescence ,Serum Albumin - Abstract
Chrysoidine is widely used in industry as a type of azo dye, and is sometimes used illegally as a food additive despite its potential toxicity. Human serum albumin (HSA) is one of the most important proteins in blood plasma and possesses major physiological functions. In the present study, the conformational and functional effects of chrysoidine on HSA were investigated by isothermal titration calorimetry (ITC), multiple spectroscopic methods, a molecular docking study and an esterase activity assay. Based on the ITC results, the binding stoichiometry of chrysoidine to HSA was estimated to be 1.5:1, and was a spontaneous process via a single hydrogen bond. The binding of chrysoidine to HSA induced dynamic quenching in fluorescence, and changes in secondary structure and in the microenvironment of the Trp-214 residue. In addition, the hydrogen bond (1.80 Å) formed between the chrysoidine molecule and the Gln-211 residue. The esterase activity of HSA decreased following the addition chrysoidine due to the change in protein structure. This study details the direct interaction between chrysoidine and HSA at the molecular level and the mechanism for toxicity as a result of the functional changes induced by HSA structural variation upon binding to chrysoidine in vitro. This study provides useful information towards detailing the transportation mechanism and toxicity of chrysoidine in vivo.
- Published
- 2015
17. Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection
- Author
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Canhua Huang, Dandan Dong, Yunlong Lei, Ke Xie, Fanghua Li, Lin Cheng, Quek Choon Lau, Rui Liu, Xudan Yang, Edouard C. Nice, Jun Zeng, and Longfei Deng
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Adult ,Male ,Oncology ,medicine.medical_specialty ,Disease-free survival ,Colorectal cancer ,Blotting, Western ,lcsh:Medicine ,General Biochemistry, Genetics and Molecular Biology ,Thyroid carcinoma ,Breast cancer ,Cell Line, Tumor ,Internal medicine ,medicine ,Adjuvant therapy ,Humans ,Overall survival ,MTT assay ,Cloning, Molecular ,CXCL14 ,Aged ,DNA Primers ,Aged, 80 and over ,Medicine(all) ,Base Sequence ,Biochemistry, Genetics and Molecular Biology(all) ,Matrigel Invasion Assay ,business.industry ,Research ,lcsh:R ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,Up-Regulation ,Colorectal carcinoma ,Female ,Neoplasm Recurrence, Local ,Colorectal Neoplasms ,business ,Chemokines, CXC - Abstract
Background The chemokine CXCL14 has been reported to play an important role in the progression of many malignancies such as breast cancer and papillary thyroid carcinoma, but the role of CXCL14 in colorectal carcinoma (CRC) remains to be established. The purpose of this study was to investigate the expression pattern and significance of CXCL14 in CRC progression. Method 265 colorectal carcinoma specimens and 129 matched adjacent normal colorectal mucosa specimens were collected. Expression of CXCL14 in clinical samples was examined by immunostaining. The effect of CXCL14 on colorectal carcinoma cell proliferation was measured by MTT assay, BrdU incorporation assay and colony formation assay. The impact of CXCL14 on migration and invasion of colorectal carcinoma cells was determined by transwell assay and Matrigel invasion assay, respectively. Results CXCL14 expression was significantly up-regulated in tumor tissues compared with adjacent nontumorous mucosa tissues (P P = 0.024). Elevated CXCL14 expression in tumor specimens (n = 135) from stage III/IV patients correlated with worse overall survival (risk ratio, 3.087; 95% CI, 1.866-5.107; P Conclusion Taken together, CXCL14 might be a potential novel prognostic factor to predict the disease recurrence and overall survival and could be a potential target of postoperative adjuvant therapy in CRC patients.
- Published
- 2013
18. Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection.
- Author
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Jun Zeng, Xudan Yang, Lin Cheng, Rui Liu, Yunlong Lei, Dandan Dong, Fanghua Li, Quek Choon Lau, Longfei Deng, Nice, Edouard C., Ke Xie, and Canhua Huang
- Subjects
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CHEMOKINES , *PROGNOSIS , *COLON cancer , *CELL proliferation , *MUCOUS membranes , *SURGICAL excision - Abstract
Background: The chemokine CXCL14 has been reported to play an important role in the progression of many malignancies such as breast cancer and papillary thyroid carcinoma, but the role of CXCL14 in colorectal carcinoma (CRC) remains to be established. The purpose of this study was to investigate the expression pattern and significance of CXCL14 in CRC progression. Method: 265 colorectal carcinoma specimens and 129 matched adjacent normal colorectal mucosa specimens were collected. Expression of CXCL14 in clinical samples was examined by immunostaining. The effect of CXCL14 on colorectal carcinoma cell proliferation was measured by MTT assay, BrdU incorporation assay and colony formation assay. The impact of CXCL14 on migration and invasion of colorectal carcinoma cells was determined by transwell assay and Matrigel invasion assay, respectively. Results: CXCL14 expression was significantly up-regulated in tumor tissues compared with adjacent nontumorous mucosa tissues (P < 0.001). Tumoral CXCL14 expression levels were significantly correlated with TNM (Tumor-nodemetastasis) stage, histodifferentiation, and tumor size. In multivariate Cox regression analysis, high CXCL14 expression in tumor specimens (n = 91) from stage I/II patients was associated with increased risk for disease recurrence (risk ratio, 2.92; 95% CI, 1.15-7.40; P = 0.024). Elevated CXCL14 expression in tumor specimens (n = 135) from stage III/IV patients correlated with worse overall survival (risk ratio, 3.087; 95% CI, 1.866-5.107; P < 0.001). Functional studies demonstrated that enforced expression of CXCL14 in SW620 colorectal carcinoma cells resulted in more aggressive phenotypes. In contrast, knockdown of CXCL14 expression could mitigate the proliferative, migratory and invasive potential of HCT116 colorectal carcinoma cells. Conclusion: Taken together, CXCL14 might be a potential novel prognostic factor to predict the disease recurrence and overall survival and could be a potential target of postoperative adjuvant therapy in CRC patients. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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