190 results on '"Wenxia Zhou"'
Search Results
2. PC3T: a signature-driven predictor of chemical compounds for cellular transition
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Lu Han, Bin Song, Peilin Zhang, Zhi Zhong, Yongxiang Zhang, Xiaochen Bo, Hongyang Wang, Yong Zhang, Xiuliang Cui, and Wenxia Zhou
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Biology (General) ,QH301-705.5 - Abstract
Abstract Cellular transitions hold great promise in translational medicine research. However, therapeutic applications are limited by the low efficiency and safety concerns of using transcription factors. Small molecules provide a temporal and highly tunable approach to overcome these issues. Here, we present PC3T, a computational framework to enrich molecules that induce desired cellular transitions, and PC3T was able to consistently enrich small molecules that had been experimentally validated in both bulk and single-cell datasets. We then predicted small molecule reprogramming of fibroblasts into hepatic progenitor-like cells (HPLCs). The converted cells exhibited epithelial cell-like morphology and HPLC-like gene expression pattern. Hepatic functions were also observed, such as glycogen storage and lipid accumulation. Finally, we collected and manually curated a cell state transition resource containing 224 time-course gene expression datasets and 153 cell types. Our framework, together with the data resource, is freely available at http://pc3t.idrug.net.cn/ . We believe that PC3T is a powerful tool to promote chemical-induced cell state transitions.
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- 2023
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3. Gender differences in mortality following tanscatheter aortic valve replacement (TAVR): a single-centre retrospective analysis from China
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Qi Liu, Yali Wang, Yijian Li, Tianyuan Xiong, Fei Chen, Yuanweixiang Ou, Xi Wang, Yijun Yao, Kaiyu Jia, Yujia Liang, Xin Wei, Xi Li, Yong Peng, Jiafu Wei, Sen He, Qiao Li, Wei Meng, Guo Chen, Wenxia Zhou, Mingxia Zheng, Xuan Zhou, Zhengang Zhao, Chen Mao, Feng Yuan, and Yanjie Yin
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Medicine - Published
- 2023
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4. Liuwei Dihuang formula ameliorates chronic stress-induced emotional and cognitive impairments in mice by elevating hippocampal O-GlcNAc modification
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Yan Huang, Jianhui Wang, Feng Liu, Chenran Wang, Zhiyong Xiao, and Wenxia Zhou
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O-GlcNAc ,Liuwei Dihuang formula ,gut microbiota ,O-GlcNAcase ,O-GlcNAc transferase ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
A substantial body of evidence has indicated that intracerebral O-linked N-acetyl-β-D-glucosamine (O-GlcNAc), a generalized post-translational modification, was emerging as an effective regulator of stress-induced emotional and cognitive impairments. Our previous studies showed that the Liuwei Dihuang formula (LW) significantly improved the emotional and cognitive dysfunctions in various types of stress mouse models. In the current study, we sought to determine the effects of LW on intracerebral O-GlcNAc levels in chronic unpredictable mild stress (CUMS) mice. The dynamic behavioral tests showed that anxiety- and depression-like behaviors and object recognition memory of CUMS mice were improved in a dose-dependent manner after LW treatment. Moreover, linear discriminate analysis (LEfSe) of genera abundance revealed a significant difference in microbiome among the study groups. LW showed a great impact on the relative abundance of these gut microbiota in CUMS mice and reinstated them to control mouse levels. We found that LW potentially altered the Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) biosynthesis process, and the abundance of O-GlcNAcase (OGA) and O-GlcNAc transferase (OGT) in CUMS mice, which was inferred using PICRUSt analysis. We further verified advantageous changes in hippocampal O-GlcNAc modification of CUMS mice following LW administration, as well as changes in the levels of OGA and OGT. In summary, LW intervention increased the levels of hippocampal O-GlcNAc modification and ameliorated the emotional and cognitive impairments induced by chronic stress in CUMS mice. LW therefore could be considered a potential prophylactic and therapeutic agent for chronic stress.
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- 2023
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5. What can traditional Chinese medicine do for adult neurogenesis?
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Wei Shen, Ning Jiang, and Wenxia Zhou
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adult neurogenesis ,neural stem cells ,traditional Chinese medicine ,TCM prescriptions ,Chinese herbal medicine ,bioactive components ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Adult neurogenesis plays a crucial role in cognitive function and mood regulation, while aberrant adult neurogenesis contributes to various neurological and psychiatric diseases. With a better understanding of the significance of adult neurogenesis, the demand for improving adult neurogenesis is increasing. More and more research has shown that traditional Chinese medicine (TCM), including TCM prescriptions (TCMPs), Chinese herbal medicine, and bioactive components, has unique advantages in treating neurological and psychiatric diseases by regulating adult neurogenesis at various stages, including proliferation, differentiation, and maturation. In this review, we summarize the progress of TCM in improving adult neurogenesis and the key possible mechanisms by which TCM may benefit it. Finally, we suggest the possible strategies of TCM to improve adult neurogenesis in the treatment of neuropsychiatric disorders.
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- 2023
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6. Stress-altering anterior insular cortex activity affects risk decision-making behavior in mice of different sexes
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Tianyao Shi, Shufang Feng, Zhonglin Zhou, Fengan Li, Yuan Fu, and Wenxia Zhou
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stress ,risk decision-making ,anterior insular cortex ,sex differences ,estrogen ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Stress can affect people’s judgment and make them take risky decisions. Abnormal decision-making behavior is a core symptom of psychiatric disorders, such as anxiety, depression, and substance abuse. However, the neuronal mechanisms underlying such impairments are largely unknown. The anterior insular cortex (AIC) is a crucial structure to integrate sensory information with emotional and motivational states. These properties suggest that AIC can influence a subjective prediction in decision-making. In this study, we demonstrated that stressed mice prefer to take more risky choices than control mice using a gambling test. Manipulating the neural activity of AIC or selectively inhibiting the AIC-BLA pathway with chemogenetic intervention resulted in alterations in risk decision-making in mice. Different sexes may have different decision-making strategies in risky situations. Endogenous estrogen levels affect emotional cognition by modulating the stress system function in women. We observed decision-making behavior in mice of different sexes with or without stress experience. The result showed that female mice did not change their choice strategy with increasing risk/reward probability and performed a lower risk preference than male mice after stress. Using the pharmacological method, we bilaterally injected an estrogen receptor (ER) antagonist that resulted in more risky behavior and decreased synaptic plasticity in the AIC of female mice. Our study suggested that the AIC is a crucial region involved in stress-induced alteration of decision-making, and estrogen in the AIC may regulate decision-making behavior by regulating synaptic plasticity.
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- 2023
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7. A touchscreen-based paradigm to measure visual pattern separation and pattern completion in mice
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Hao Wang, Na Sun, Xinyue Wang, Jinyuan Han, Yongxiang Zhang, Yan Huang, and Wenxia Zhou
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memory accuracy ,pattern separation ,pattern completion ,rodents ,pairwise discrimination task ,touchscreen ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Memory accuracy involves two major processes: pattern separation and pattern completion. Pattern separation refers to the ability to reduce overlap among similar inputs to avoid interference, and pattern completion refers to the ability to retrieve the whole information from partial or degraded cues. Impairments in pattern separation/pattern completion contribute to cognitive deficits in several diseases of the nervous system. Therefore, it is better to evaluate both pattern separation and pattern completion in one apparatus. However, few tools are available to assess pattern separation and pattern completion within the same apparatus for rodents. In this study, we designed a series of images with varying degrees of similarity to the correct image to evaluate pattern separation and pattern completion. First, mice were trained to discriminate between two totally different images, and once the correct percentage reached above 77% for two consecutive days, the images with different degrees of similarity were used to measure pattern separation and pattern completion. The results showed the mice performed progressively worse from S0 to S4 (increasing similarity) when discriminating similar images in pattern separation, and the mice performed progressively worse from C0 to C4 (decreasing cues information) when recalling the correct image according to partial cues in pattern completion, implying a good image similarity-dependent manner for memory accuracy evaluation. In sum, we designed a convenient, effective paradigm to evaluate pattern separation and pattern completion based on a touchscreen pairwise discrimination task, which may provide a new method for the studies of the effects and mechanisms of memory accuracy enhancing drugs.
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- 2022
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8. Various Energetic Metabolism of Microglia in Response to Different Stimulations
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Xiaohui Liu, Ning Jiang, and Wenxia Zhou
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Alzheimer’s disease ,microglia ,DAMPs ,PAMPs ,cytokines ,energetic metabolism ,Organic chemistry ,QD241-441 - Abstract
The activation of the microglia plays an important role in the neuroinflammation induced by different stimulations associated with Alzheimer’s disease (AD). Different stimulations, such as pathogen-associated molecular patterns (PAMPs), damage-associated molecular patterns (DAMPs) and cytokines, trigger a consequence of activation in the microglia with diverse changes of the microglial cell type response in AD. The activation of the microglia is often accompanied by metabolic changes in response to PAMPs, DAMPs and cytokines in AD. Actually, we do not know the distinct differences on the energetic metabolism of microglia when subject to these stimuli. This research assessed the changes of the cell type response and energetic metabolism in mouse-derived immortalized cells (BV-2 cells) induced by a PAMP (LPS), DAMPs (Aβ and ATP) and a cytokine (IL-4) in mouse-derived immortalized cells (BV-2 cells) and whether the microglial cell type response was improved by targeting the metabolism. We uncovered that LPS, a proinflammatory stimulation of PAMPs, modified the morphology from irregular to fusiform, with stronger cell viability, fusion rates and phagocytosis in the microglia accompanied by a metabolic shift to the promotion of glycolysis and the inhibition of oxidative phosphorylation (OXPHOS). Aβ and ATP, which are two known kinds of DAMPs that trigger microglial sterile activation, induced the morphology from irregular to amoebic, and significantly decreased others in the microglia, accompanied by boosting or reducing both glycolysis and OXPHOS. Monotonous pathological changes and energetic metabolism of microglia were observed under IL-4 exposure. Further, the inhibition of glycolysis transformed the LPS-induced proinflammatory morphology and decreased the enhancement of LPS-induced cell viability, the fusion rate and phagocytosis. However, the promotion of glycolysis exerted a minimal effect on the changes of morphology, the fusion rate, cell viability and phagocytosis induced by ATP. Our study reveals that microglia induced diverse pathological changes accompanied by various changes in the energetic metabolism in response to PAMPs, DAMPs and cytokines, and it may be a potential application of targeting the cellular metabolism to interfere with the microglia-mediated pathological changes in AD.
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- 2023
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9. A modified mouse model for observational fear learning and the influence of social hierarchy
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Tianyao Shi, Shufang Feng, Wenlong Shi, Yuan Fu, and Wenxia Zhou
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observational fear ,emotionality ,integrated Z-score ,susceptibility ,social hierarchy ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundIndirectly experiencing traumatic events either by witnessing or learning of a loved one’s suffering is associated with the highest prevalence rates of epidemiological features of PTSD. Social species can develop fear by observing conspecifics in distress. Observational fear learning (OFL) is one of the most widely used paradigms for studying fear contagion in mice. However, the impact of empathic fear behavior and social hierarchy on fear transfer in mice is not well understood.MethodsFear emotions are best characterized in mice by using complementary tests, rather than only freezing behavior, and simultaneously avoiding behavioral variability in different tests across time. In this study, we modified the OFL model by implementing freezing (FZ), open field (OF), and social interaction (SI) tests in a newly designed experimental facility and applied Z-normalization to assess emotionality changes across different behaviors.ResultsThe integrated emotionality scores revealed a robustly increased emotionality of observer mice and, more importantly, contributed to distinguishing susceptible individuals. Interestingly, fos-positive neurons were mainly found in the interoceptive network, and mice of a lower social rank showed more empathy-like behaviors.ConclusionOur findings highlight that combining this experimental model with the Z-scoring method yields robust emotionality measures of individual mice, thus making it easier to screen and differentiate between empathic fear-susceptible mice and resilient mice, and refining the translational applicability of these models.
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- 2022
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10. Deep learning applications for the accurate identification of low-transcriptional activity drugs and their mechanism of actions
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Shengqiao Gao, Lu Han, Dan Luo, Zhiyong Xiao, Gang Liu, Yongxiang Zhang, and Wenxia Zhou
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Gene expression profiles ,Deep learning ,Low transcriptional activity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Analysis of drug-induced expression profiles facilitated comprehensive understanding of drug properties. However, many compounds exhibit weak transcription responses though they mostly possess definite pharmacological effects. Actually, as a representative example, over 66.4% of 312,438 molecular signatures in the Library of Integrated Cellular Signatures (LINCS) database exhibit low-transcriptional activities (i.e. TAS-low signatures). When computing the association between TAS-low signatures with shared mechanism of actions (MOAs), commonly used algorithms showed inadequate performance with an average area under receiver operating characteristic curve (AUROC) of 0.55, but the computation accuracy of the same task can be improved by our developed tool Genetic profile activity relationship (GPAR) with an average AUROC of 0.68. Up to 36 out of 74 TAS-low MOAs were well trained with AUROC ≥ 0.7 by GPAR, higher than those by other approaches. Further studies showed that GPAR benefited from the size of training samples more significantly than other approaches. Lastly, in biological validation of the MOA prediction for a TAS-low drug Tropisetron, we found an unreported mechanism that Tropisetron can bind to the glucocorticoid receptor. This study indicated that GPAR can serve as an effective approach for the accurate identification of low-transcriptional activity drugs and their MOAs, thus providing a good tool for drug repurposing with both TAS-low and TAS-high signatures.
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- 2022
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11. Different Synaptic Plasticity After Physiological and Psychological Stress in the Anterior Insular Cortex in an Observational Fear Mouse Model
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Wenlong Shi, Yuan Fu, Tianyao Shi, and Wenxia Zhou
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psychological stress ,physiological stress ,anterior insular cortex ,long-term potentiation ,long-term depression ,multielectrode array ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Post-traumatic stress disorder (PTSD) can be triggered not only in people who have personally experienced traumatic events but also in those who witness them. Physiological and psychological stress can have different effects on neural activity, but little is known about the underlying mechanisms. There is ample evidence that the insular cortex, especially the anterior insular cortex (aIC), is critical to both the sensory and emotional experience of pain. It is therefore worthwhile to explore the effects of direct and indirect stress on the synaptic plasticity of the aIC. Here, we used a mouse model of observational fear to mimic direct suffering (Demonstrator, DM) and witnessing (Observer, OB) of traumatic events. After observational fear training, using a 64-channel recording system, we showed that both DM and OB mice exhibited a decreased ratio of paired-pulse with intervals of 50 ms in the superficial layers of the aIC but not in the deep layers. We found that theta-burst stimulation (TBS)–induced long-term potentiation (LTP) in OB mice was significantly higher than in DM mice, and the recruitment of synaptic responses occurred only in OB mice. Compared with naive mice, OB mice showed stronger recruitment and higher amplitude in the superficial layers of the aIC. We also used low-frequency stimulation (LFS) to induce long-term depression (LTD). OB mice showed greater LTD in both the superficial and deep layers of the aIC than naive mice, but no significant difference was found between OB and DM mice. These results provide insights into the changes in synaptic plasticity in the aIC after physiological and psychological stress, and suggest that different types of stress may have different mechanisms. Furthermore, identification of the possible causes of the differences in stress could help treat stress-related disorders.
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- 2022
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12. How Psychological Capital Impacts Career Growth of University Students? The Role of Academic Major Choice
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Wenxia Zhou, Yue Feng, and Qiuping Jin
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This study aimed to investigate whether and how university students with high psychological capital (PsyCap) experience more career growth. Based on self-determination theory, we examined the mediating role of self-concordance in choosing majors and the moderating role of career values in this relationship. Results from a two-wave lagged study of 705 Chinese university students over one year provided support for our model. We found that students' PsyCap upon university entrance was positively related to their career growth one year later through the mediating role of self-concordance in choosing majors; extrinsic career values (external compensation and work-life balance values) mitigated the positive effect of self-concordance in choosing majors on career growth, as well as the indirect effect. We contribute by identifying PsyCap as an antecedent of career growth of university students and by revealing its influence mechanisms in a career choice scenario. Practical implications of the study are also discussed.
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- 2024
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13. Modeling drug mechanism of action with large scale gene-expression profiles using GPAR, an artificial intelligence platform
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Shengqiao Gao, Lu Han, Dan Luo, Gang Liu, Zhiyong Xiao, Guangcun Shan, Yongxiang Zhang, and Wenxia Zhou
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MOA ,Deep learning ,Gene expression profiles ,L1000 ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Querying drug-induced gene expression profiles with machine learning method is an effective way for revealing drug mechanism of actions (MOAs), which is strongly supported by the growth of large scale and high-throughput gene expression databases. However, due to the lack of code-free and user friendly applications, it is not easy for biologists and pharmacologists to model MOAs with state-of-art deep learning approach. Results In this work, a newly developed online collaborative tool, Genetic profile-activity relationship (GPAR) was built to help modeling and predicting MOAs easily via deep learning. The users can use GPAR to customize their training sets to train self-defined MOA prediction models, to evaluate the model performances and to make further predictions automatically. Cross-validation tests show GPAR outperforms Gene set enrichment analysis in predicting MOAs. Conclusion GPAR can serve as a better approach in MOAs prediction, which may facilitate researchers to generate more reliable MOA hypothesis.
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- 2021
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14. Good or Bad? The Ambivalent Leader-Follower Relationships
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Qinglin Zhao and Wenxia Zhou
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ambivalent relationships ,paradox view ,conservation of resources theory ,workplace relationships ,stressor ,Psychology ,BF1-990 - Abstract
Researchers have emphasized the positive and negative influences of ambivalent leader-follower relationships, but it is not clear when the ambivalent relationship is associated with good or bad influences. To answer this question, we reviewed the definition and identified 10 different types of ambivalent leader-follower relationships. Further, we demonstrate that the negative outcomes (more inflexibility, disengagement, and worse performance) can be explained by the workplace stressor perspective, and that the positive outcomes (more flexibility, engagement, and better performance) can be explained by paradox view. Finally, drawing from conservation of resources (COR) theory, we integrate workplace stressor framework and the paradox view to address when the ambivalent leader-follower relationship is beneficial or detrimental for followers. We proposed that the degree of ambivalence, support from the third party, and integrative complexity of follower will influence the possible positive or negative influences. Limitations and future directions were also discussed.
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- 2021
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15. Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
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Lin Zhang, Qiao Ren, Xiaohui Liu, Gang Liu, Shulin Meng, Kang Xiahou, Yongxiang Zhang, Ning Jiang, and Wenxia Zhou
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Biology (General) ,QH301-705.5 - Abstract
Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis.
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- 2021
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16. Establishment of two induced pluripotent stem cell line from healthy elderly (IPTi005-A and IPTi007-A)
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Lin Zhang, Qiao Ren, Gang Liu, Rongrong Wu, Shulin Meng, Kang Xiahou, Ning Jiang, Yongxiang Zhang, and Wenxia Zhou
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Biology (General) ,QH301-705.5 - Abstract
Two healthy elderly donated their peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell lines (IPTi005-A and IPTi007-A) were reprogrammed by episomal vector system with Yamanaka factors OCT4, SOX2, C-MYC and KLF4. Both the two iPSC lines can differentiate into three germ layers determined by embryoid bodies (EBs) differentiation. Flow cytometry analysis showed that more than 90% cells expressed NANOG, OCT4 and SSEA4. Besides, the iPSC lines of IPTi005-A and IPTi007-A were confirmed to exhibit a normal karyotype. In the studies of age-related disease, the iPSC lines can be used as controls.
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- 2020
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17. Comparison of Donepezil, Memantine, Melatonin, and Liuwei Dihuang Decoction on Behavioral and Immune Endocrine Responses of Aged Senescence-Accelerated Mouse Resistant 1 Mice
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Ju Zeng, Xiaorui Zhang, Jianhui Wang, Xiaorui Cheng, Yongxiang Zhang, and Wenxia Zhou
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aging ,Liuwei Dihuang decoction ,cognition ,immune response ,inflammation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Aging is a natural biological process associated with cognitive decline and neuroendocrine–immune system changes; the neuroendocrine–immune system plays crucial role in brain aging and neurodegeneration, and it is essential to discern beneficial attempts to delay the aging progress based on immunological aging. In this study, we have investigated the effects of Traditional Chinese Medicine (TCM)—Liuwei Dihuang decoction (LW)—and donepezil, memantine, and melatonin on cognitive decline in aging mice. The aged SAMR1 mice received oral administration of donepezil (1mg/kg), memantine (10 mg/kg), melatonin (10 mg/kg), and LW (10 g/kg) for 3 months. A shuttle box, Morris water maze, and elevated-zero maze were performed to assess cognitive function, and flowcytometry, Luminex, and radioimmunoassay were performed to measure the lymphocyte subsets, inflammatory factors, and hormones. We observed that survival days of mice was increased with melatonin and LW, the anxiety behavior was significantly improved by memantine, melatonin, and LW treatment, active avoidance responses significantly improved by LW, donepezil, and memantine, the spatial learning ability was significantly improved by donepezil, and LW and melatonin were beneficial to the spatial memory of old mice. For immune function, LW increased CD4+ and CD4+CD28+ cells and reduced TNF-α, IL-1β, and G-CSF in plasma, and it also promoted the secretion of anti-inflammatory factors IL-4, IL-5, and IL-10 by regulating the active of Th2 cells in spleen. Donepezil and memantine exerted protective effects against CD4+CD28+ cell decrease caused by aging and reduced the pro-inflammatory factors TNF-α, IL-1β, and G-CSF in plasma. Melatonin could reverse CD8+CD28+ cell imbalances and increased B cells. For endocrine factors, LW increased TSH levels in the pituitary, and melatonin increased the GH level in blood. Our findings indicated that LW improved the cognitive decline in aging mice, and this might be associated with modulation of the active T cells and HPG axis hormones as well as increasing anti-inflammatory factors. Meanwhile, donepezil and memantine have advantages in regulating adaptive immunity, melatonin has advantages in the regulation of B cells and pituitary hormones, and LW exhibits a better effect on neuroendocrine immune function compared with the others from a holistic point of view. LW might be a potential therapeutic strategy for anti-aging-related syndromes, and it can also provide a value on medication guidance about drug combinations or treatment in clinic.
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- 2020
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18. Career Success Criteria Clarity as a Predictor of Employment Outcomes
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Lu Xin, Wenxia Zhou, Mengyi Li, and Fangcheng Tang
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career success ,career satisfaction ,person–job fit ,well-being ,career decision-making self-efficacy ,Psychology ,BF1-990 - Abstract
Drawing on the goal-setting theory and social cognitive career theory (SCCT), this study empirically proposes an operational definition of career success criteria clarity (CSCC) and further explores its impact on career satisfaction, person–job fit, and subjective well-being through the mediating role of career decision-making self-efficacy (CDSE). A pilot study of 231 samples showed that the CSCC scale had good reliability and validity. To further test the effects of CSCC on crucial employment outcomes, as well as the mediating role of CDSE, 240 employees were included in an additional survey. Structural equation modeling path analysis supported all the expected hypotheses. Results indicated that: (1) CSCC was positively correlated to career satisfaction; (2) CSCC was positively correlated to person–job fit; (3) CSCC was positively correlated to subjective well-being; (4) CSCC was positively correlated to CDSE; (5) CDSE fully mediated the relationship between CSCC and career satisfaction; (6) CDSE fully mediated the relationship between CSCC and person–job fit; and (7) CDSE partly mediated the relationship between CSCC and subjective well-being. The results contributed to social cognitive career theory model and provided suggestions for both the career educators and consultants.
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- 2020
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19. Generation of induced pluripotent stem cell line (IPTi001-A) from a 62-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
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Lin Zhang, Mei Xu, Gang Liu, Rongrong Wu, Shulin Meng, Kang Xiahou, Ning Jiang, Yongxiang Zhang, and Wenxia Zhou
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Biology (General) ,QH301-705.5 - Abstract
A 62-year old sporadic Alzheimer's disease (sAD) male patient with APOE3 (ε3/ε3) genotype donated his peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell (iPSC) line was established by episomal vector system with the four factors OCT4, SOX2, C-MYC and KLF4. EB differentiation in vitro showed that iPSC line had a potential to differentiate into three germ layers. More than 90% cells expressed NANOG, OCT4 and SSEA4 detected by flow cytometry. In addition, the iPSC line was karyotypically normal. The iPSC line may provide new valuable tools for studying pathogenesis of sAD and screening candidate drugs for the disease.
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- 2019
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20. LINCS Dataset-Based Repositioning of Dutasteride as an Anti-Neuroinflammation Agent
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Dan Luo, Lu Han, Shengqiao Gao, Zhiyong Xiao, Qingru Zhou, Xiaorui Cheng, Yongxiang Zhang, and Wenxia Zhou
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neuroinflammation ,cognitive impairment ,drug repurposing ,LINCS ,dutasteride ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Neuroinflammation is often accompanied by central nervous system (CNS) injury seen in various CNS diseases, with no specific treatment. Drug repurposing is a strategy of finding new uses for approved or investigational drugs, and can be enabled by the Library of Integrated Network-based Cellular Signatures (LINCS), a large drug perturbation database. In this study, the signatures of Lipopolysaccharide (LPS) were compared with the signatures of compounds contained in the LINCS dataset. To the top 100 compounds obtained, the Quantitative Structure-Activity Relationship (QSAR)-based tool admetSAR was used to identify the top 10 candidate compounds with relatively high blood–brain barrier (BBB) penetration. Furthermore, the seventh-ranked compound, dutasteride, a 5-α-reductase inhibitor, was selected for in vitro and in vivo validation of its anti-neuroinflammation activity. The results showed that dutasteride significantly reduced the levels of IL-6 and TNF-α in the supernatants of LPS-stimulated BV2 cells, and decreased the levels of IL-6 in the hippocampus and plasma, and the number of activated microglia in the brain of LPS administration mice. Furthermore, dutasteride also attenuated the cognitive impairment caused by LPS stimulation in mice. Taken together, this study demonstrates that the LINCS dataset-based drug repurposing strategy is an effective approach, and the predicted candidate, dutasteride, has the potential to ameliorate LPS-induced neuroinflammation and cognitive impairment.
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- 2021
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21. What Drives the Trickle-Down Effect of Calling Orientation From Supervisors to Subordinates? The Perspective of Social Learning Theory
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Baoguo Xie, Wenxia Zhou, De Xia, and Yongxing Guo
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trickle-down ,social learning theory ,calling orientation ,role modeling ,organizational status ,Psychology ,BF1-990 - Abstract
Despite an increase in research on calling orientation, few studies have investigated its antecedents. Drawing on social learning theory, we hypothesized that subordinates’ perceptions of their supervisor’s role modeling mediate the relationship between supervisor’s and subordinates’ calling orientations. Supervisor’s organizational status is supposed to augment the trickle-down process for calling orientation. We used multilevel modeling to test these hypotheses in a sample of 738 subordinates nested in 77 work teams in Chinese firm. We found that supervisor’s calling orientation was positively related to subordinate’s calling orientation and that the relationship was fully mediated by subordinates’ perceptions of role modeling. Additionally, the relationship between supervisor’s calling orientation and subordinates’ calling orientation via role modeling was moderated by supervisor’s organizational status at the second stage.
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- 2019
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22. Combine Your 'Will' and 'Able': Career Adaptability’s Influence on Performance
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Xueyuan Gao, Xun Xin, Wenxia Zhou, and Denise M. Jepsen
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career adaptability ,proactive personality ,career self-management ,performance ,career construction theory ,Psychology ,BF1-990 - Abstract
Adaptivity and adaptability are two key elements representing one’s “willingness” and “ability,” respectively, in the career construction theory (CCT) framework. On the basis of CCT and complemented by the visual of resources in the conservation of resources theory, this study combines career issues and performance and examines the joint effect of adaptivity and adaptability on career self-management which will lead to improved performance. Using a sample of Chinese employees (N = 232), the study first examines the mediating role that career self-management plays between career adaptability and performance and then tests the moderating role of proactive personality. Results show career adaptability positively predicts performance, with this relationship partially mediated by career self-management. The positive effect of career adaptability on career self-management is stronger among those who are more proactive than less proactive. Further, the indirect effect of career adaptability on performance is stronger among proactive employees than those with lower levels of proactive personality. These findings provide implications for both theories and practices.
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- 2019
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23. The Cytochrome P450-Mediated Metabolism Alternation of Four Effective Lignans From Schisandra chinensis in Carbon Tetrachloride-Intoxicated Rats and Patients With Advanced Hepatocellular Carcinoma
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Rongrong Wu, Zhiyong Xiao, Xiaorui Zhang, Feng Liu, Wenxia Zhou, and Yongxiang Zhang
- Subjects
Schisandra chinensis (SC) ,lignans ,pharmacokinetics ,liver injury ,cytochrome P450(CYP450) ,Therapeutics. Pharmacology ,RM1-950 - Abstract
It is highly valuable to study the pharmacokinetics of herbal components under the pathological condition of liver dysfunction for safe and rational use of herbal medicines. In this study, the pharmacokinetic profiles of four effective lignans from Schisandra chinensis (SC), schisandrin, schisantherin A, deoxyshisandrin and γ-schisandrin, were investigated in carbon tetrachloride (CCl4)-intoxicated rats. The metabolism of the four lignans was also studied using microsomes from patients with advanced hepatocellular carcinoma. In situ intestinal and hepatic perfusions were conducted to clarify the contributions from impairments of gut and liver on the pharmacokinetics of the four schisandra lignans in CCl4-intoxicated rats. The metabolism in rat and human liver microsomes and transport in Caco-2 monolayer cell model were studied to reveal the key factors for the in vivo disposition of the four lignans. When SC alcoholic extract was orally administrated to CCl4-intoxicated rat for a short term (4 days), the pharmacokinetics of four active SC lignans was significantly changed while its hepatotherapeutic effect was not obviously observed. The plasma concentrations of the four schisandra lignans were dramatically elevated compared with the control. The Cmax, AUC and MRT were all increased or prolonged significantly while parameter CLz/F was obviously reduced in rat pretreated with CCl4. In hepatic perfusion study and liver microsomes incubation, it was found that the hepatic metabolism of the four lignans was markedly decreased mainly due to the activity reduction of multiple CYP450 isoenzymes involved the metabolism, which, eventually, might lead to the alternation of their pharmacokinetic profiles in CCl4-intoxicated rats or patients with advanced hepatocellular carcinoma. The pharmacokinetic studies of SC components in pathological situation of liver dysfunction are expected to provide useful data for rational and safe application of SC preparations in clinic or further pharmacological and toxicological research.
- Published
- 2018
- Full Text
- View/download PDF
24. Knowledge-Based Neuroendocrine Immunomodulation (NIM) Molecular Network Construction and Its Application
- Author
-
Tongxing Wang, Lu Han, Xiaorui Zhang, Rongrong Wu, Xiaorui Cheng, Wenxia Zhou, and Yongxiang Zhang
- Subjects
neuroendocrine immunomodulation network ,disease network ,rheumatoid arthritis ,Alzheimer’s disease ,drug target ,pathogenesis ,Organic chemistry ,QD241-441 - Abstract
Growing evidence shows that the neuroendocrine immunomodulation (NIM) network plays an important role in maintaining and modulating body function and the homeostasis of the internal environment. The disequilibrium of NIM in the body is closely associated with many diseases. In the present study, we first collected a core dataset of NIM signaling molecules based on our knowledge and obtained 611 NIM signaling molecules. Then, we built a NIM molecular network based on the MetaCore database and analyzed the signaling transduction characteristics of the core network. We found that the endocrine system played a pivotal role in the bridge between the nervous and immune systems and the signaling transduction between the three systems was not homogeneous. Finally, employing the forest algorithm, we identified the molecular hub playing an important role in the pathogenesis of rheumatoid arthritis (RA) and Alzheimer’s disease (AD), based on the NIM molecular network constructed by us. The results showed that GSK3B, SMARCA4, PSMD7, HNF4A, PGR, RXRA, and ESRRA might be the key molecules for RA, while RARA, STAT3, STAT1, and PSMD14 might be the key molecules for AD. The molecular hub may be a potentially druggable target for these two complex diseases based on the literature. This study suggests that the NIM molecular network in this paper combined with the forest algorithm might provide a useful tool for predicting drug targets and understanding the pathogenesis of diseases. Therefore, the NIM molecular network and the corresponding online tool will not only enhance research on complex diseases and system biology, but also promote the communication of valuable clinical experience between modern medicine and Traditional Chinese Medicine (TCM).
- Published
- 2018
- Full Text
- View/download PDF
25. New Conotoxin SO-3 Targeting N-type Voltage-Sensitive Calcium Channels
- Author
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Lei Wen, Sheng Yang, Wenxia Zhou, Peitang Huang, and Yongxiang Zhang
- Subjects
É-conotoxins ,SO-3 ,MVIIA ,voltage-sensitive calcium channels ,N-type calcium channel blockers ,pain ,Biology (General) ,QH301-705.5 - Abstract
Selective blockers of the N-type voltage-sensitive calcium (CaV) channels are useful in the management of severe chronic pain. Here, the structure and function characteristics of a novel N-type CaV channel blocker, SO-3, are reviewed. SO-3 is a 25-amino acid conopeptide originally derived from the venom of Conus striatus, and contains the same 4-loop, 6-cysteine framework (C-C-CC-C-C) as O-superfamily conotoxins. The synthetic SO-3 has high analgesic activity similar to É-conotoxin MVIIA (MVIIA), a selective N-type CaV channel blocker approved in the USA and Europe for the alleviation of persistent pain states. In electrophysiological studies, SO-3 shows more selectivity towards the N-type CaV channels than MVIIA. The dissimilarity between SO-3 and MVIIA in the primary and tertiary structures is further discussed in an attempt to illustrate the difference in selectivity of SO-3 and MVIIA towards N-type CaV channels.
- Published
- 2006
- Full Text
- View/download PDF
26. Design, Synthesis, and Biological Evaluation of Novel PARP-1 Inhibitors Based on a 1H-Thieno[3,4-d] Imidazole-4-Carboxamide Scaffold
- Author
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Lingxiao Wang, Feng Liu, Ning Jiang, Wenxia Zhou, Xinbo Zhou, and Zhibing Zheng
- Subjects
PARP-1 inhibitor ,BRCA1/2 ,anti-tumour ,thieno[3,4-d]imidazole ,Organic chemistry ,QD241-441 - Abstract
A series of poly(ADP-ribose)polymerase (PARP)-1 inhibitors containing a novel scaffold, the 1H-thieno[3,4-d]imidazole-4-carboxamide moiety, was designed and synthesized. These efforts provided some compounds with relatively good PARP-1 inhibitory activity, and among them, 16l was the most potent one. Cellular evaluations indicated that the anti-proliferative activities of 16g, 16i, 16j and 16l against BRCA-deficient cell lines were similar to that of olaparib, while the cytotoxicities of 16j and 16l toward human normal cells were lower. In addition, ADMET prediction results indicated that these compounds might possess more favorable toxicity and pharmacokinetic properties. This study provides a basis for our further investigation.
- Published
- 2016
- Full Text
- View/download PDF
27. siGCD: a web server to explore survival interaction of genes, cells and drugs in human cancers.
- Author
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Xiuliang Cui, Lu Han, Yang Liu, Ying Li, Wen Sun, Bin Song, Wenxia Zhou, Yongxiang Zhang, and Hongyang Wang
- Published
- 2021
- Full Text
- View/download PDF
28. Accelerating drug repurposing for COVID-19 via modeling drug mechanism of action with large scale gene-expression profiles.
- Author
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Lu Han, Guangcun Shan, H. Y. Wang, Shengqiao Gao, and Wenxia Zhou
- Published
- 2020
29. Influence of gut microbiota on resilience and its possible mechanisms
- Author
-
Jianhui Wang, Ting Zhou, Feng Liu, Yan Huang, Zhiyong Xiao, Yan Qian, and Wenxia Zhou
- Subjects
Cell Biology ,Molecular Biology ,Applied Microbiology and Biotechnology ,Ecology, Evolution, Behavior and Systematics ,Developmental Biology - Published
- 2023
30. Organizational career management in the new career era: scale development and validation
- Author
-
Wenxia Zhou, Qinglin Zhao, Nan Jiang, and Puxin Lin
- Subjects
Organizational Behavior and Human Resource Management - Published
- 2022
31. Organizational career management: a review and future prospect
- Author
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Qinglin Zhao, Zijun Cai, Wenxia Zhou, and Ledi Zang
- Subjects
Organizational Behavior and Human Resource Management ,Social Sciences (miscellaneous) - Abstract
PurposeThe purpose of this study is to review the research about organizational career management (OCM) and provide an integrated understanding of OCM research.Design/methodology/approachIn this paper, the authors systematically review 85 OCM-related papers published in highly influential journals over the past four decades (1978–2021). This paper reviews the definitions, measurements, antecedents, outcomes, mediators and moderators of OCM.FindingsDiverse definitions of OCM exist, with three key common factors: what should be included in OCM, who is responsible for OCM and the goal of OCM. Scholars use different OCM measures, which might be due to different nations, industries, groups and scale development methods. More than 20 papers demonstrated the positive influences of OCM, providing convincing evidence of the necessity of OCM. About 90% of the current papers we reviewed (27 out of 30 papers) dominantly examined the influence of OCM on individuals’ attitudes or work behavior. The influence of OCM on organizational outcomes was less addressed.Originality/valueFirst, the authors review the existing OCM measurements and distinguish two ways of measuring OCM: OCMP (organizational level, rated by HR managers or HR vice president or CEO, capturing the real practices) and POCM (Perceived OCM, individual level, rated by employees, capturing subjective perception of practices). This distinction reduces the ambiguity in existing measurements. Second, we summarize the empirical findings of OCM, including the antecedents, outcomes, mediators and moderators. These findings uncover the benefits/risks of OCM and the factors that may influence its effectiveness. Third, the review provides several practical implications as the findings can help managers improve their career development programs.
- Published
- 2022
32. A Case Study of Psychological Technique Intervention on Hyperacusis
- Author
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Jiaqi, Wu, primary, Yaying, Lv, additional, and Wenxia, Zhou, additional
- Published
- 2023
- Full Text
- View/download PDF
33. B-Raf inhibitor vemurafenib counteracts sulfur mustard-induced epidermal impairment through MAPK/ERK signaling
- Author
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Zhiyong Xiao, Feng Liu, Junping Cheng, Ying Wang, Wenxia Zhou, and Yongxiang Zhang
- Subjects
Pharmacology ,Chemical Health and Safety ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,Toxicology - Abstract
The chemical warfare agent sulfur mustard (SM) causes severe cutaneous lesions characterized by epidermal cell death, apoptosis, and inflammation. At present, the molecular mechanisms underlying SM-induced injury are not well understood, and there is no standard treatment protocol for SM-exposed patients. Here, we conducted a high-content screening of the Food and Drug Administration (FDA)-approved drug library of 1018 compounds against SM injury on an immortal human keratinocyte HaCaT cell line, focusing on cell survival. We found that the B-Raf inhibitor vemurafenib had an apparent therapeutic effect on HaCaT cells and resisted SM toxicity. Other tested B-Raf inhibitors, both type-I (dabrafenib and encorafenib) and type-II (RAF265 and AZ628), also exhibited potent therapeutic effects on SM-exposed HaCaT cells. Both SM and vemurafenib triggered extracellular signal-related kinase (ERK) activation. The therapeutic effect of vemurafenib in HaCaT cells during SM injury was ERK-dependent, indicating a specific role of ERK in keratinocyte regulatory mechanisms. Furthermore, vemurafenib partially improved cutaneous damage in a mouse ear vesicant model. Collectively, our results provide evidence that the B-Raf inhibitor vemurafenib is a potential therapeutic agent against SM injury, and oncogenic B-Raf might be an exciting new therapeutic target following exposure to mustard vesicating agents.
- Published
- 2022
34. Deletion of AhR Attenuates Fear Memory Leaving Memory Ability Intact
- Author
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Na Sun, Hao Wang, Xinyue Wang, Qi Yu, Jinyuan Han, Wenxia Zhou, and Yan Huang
- Published
- 2023
35. High-performance work systems and key employee attitudes: the roles of psychological capital and an interactional justice climate
- Author
-
Rentao Miao, Nikos Bozionelos, Wenxia Zhou, and Alexander Newman
- Published
- 2022
36. Immune index: a gene and cell prognostic signature for immunotherapy response prediction in hepatocellular carcinoma
- Author
-
Xiuliang Cui, Lu Han, Longjiu Cui, Gongbo Fu, Erdong Liu, Duowei Wang, Bin Song, Yongxiang Zhang, Wenxia Zhou, Hongyang Wang, and Jing Fu
- Subjects
Pharmacology - Abstract
The heterogeneity of tumor immune microenvironment (TIME) plays important roles in the development and immunotherapy response of hepatocellular carcinoma (HCC). Using machine learning algorithms, we introduced the immune index (IMI), a prognostic model based on the HCC immune landscape. We found that IMI low HCCs were enriched in stem cell and proliferating signatures, and yielded more TP53 mutation and 17p loss compared with IMI high HCCs. More importantly, patients with high IMI exhibited better immune-checkpoint blockade (ICB) response. To facilitate clinical application, we employed machine learning algorithms to develop a gene model of the IMI (IMI
- Published
- 2022
37. Clinical and experimental research progress on neurotoxicity of sulfur mustard and its possible mechanisms
- Author
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Ning Jiang, Pei Zhang, Wei Shen, Yongxiang Zhang, and WenXia Zhou
- Subjects
Toxicology - Abstract
Sulfur mustard (SM), an extremely reactive alkylating toxicant, which poses a continuing threat to both military and civilian populations. SM targets three major organs including skin, eyes and lungs. In recent years, more and more clinical findings have shown that cognitive and emotional disorders in veterans intoxicated with SM, such as anxiety, depression, apathy, cognitive decline and so on, which indicated the long time toxic effects on mental and neurological health of SM. The experimental studies in animal and cell models have also found neurotoxicity which are similar to clinical results. However, these neuropsychological problems are not studied well in victims of SM and the mental and neurological complications are often not subjected to treatment or undertreated. Until now, the exact mechanism of the action of SM toxicity has not been elucidated and no specific therapy for its poisoning exists. Therefore, the studies on neurotoxicity of SM should be strengthened. This review summarizes the main progress of clinical and experimental researches on neurotoxicity of SM for the past few years.
- Published
- 2022
38. Histopathological characteristics and oxidative injury secondary to atrial fibrillation in the left atrial appendages of patients with different forms of mitral valve disease
- Author
-
Yongjun, Qian, Huanzhang, Shao, Wenxia, Zhou, Hong, Tang, and Xijun, Xiao
- Published
- 2013
- Full Text
- View/download PDF
39. Special Aortic Chordae Tendineae Strand Causing Severe Aortic Regurgitation Treated by Transcatheter Aortic Valve Replacement
- Author
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Yuan Feng, WenXia Zhou, Mao Chen, and Xin Wei
- Subjects
medicine.medical_specialty ,Transcatheter aortic ,business.industry ,medicine.medical_treatment ,Aortic Valve Insufficiency ,Mitral Valve Insufficiency ,Regurgitation (circulation) ,Transcatheter Aortic Valve Replacement ,Treatment Outcome ,medicine.anatomical_structure ,Valve replacement ,Internal medicine ,medicine ,Cardiology ,Chordae Tendineae ,Humans ,Chordae tendineae ,Cardiology and Cardiovascular Medicine ,business - Published
- 2021
40. Human Induced Pluripotent Stem Cell-Derived Neural Cells from Alzheimer's Disease Patients Exhibited Different Susceptibility to Oxidative Stress
- Author
-
Wenxia Zhou, Ning Jiang, Kang Xiahou, Mei Xu, Shulin Meng, Yongxiang Zhang, Qiao Ren, Gang Liu, and Lin Zhang
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Neurite ,Cell Survival ,Phagocytosis ,Induced Pluripotent Stem Cells ,Neuronal Outgrowth ,Stimulation ,Biology ,medicine.disease_cause ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Neural Stem Cells ,Alzheimer Disease ,Internal medicine ,medicine ,Humans ,Induced pluripotent stem cell ,Neurons ,chemistry.chemical_classification ,Reactive oxygen species ,Microglia ,Hydrogen Peroxide ,Cell Biology ,Hematology ,Neural stem cell ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,nervous system ,chemistry ,Cytokines ,030217 neurology & neurosurgery ,Oxidative stress ,Developmental Biology - Abstract
The cell-type-specific response of neural cells to oxidative stress, a crucial mechanism for accelerating aging and cognitive dysfunction in Alzheimer's disease (AD), is still far from understood. Here, we employed human-induced pluripotent stem cells (hiPSCs)-derived neural stem cells (hiPSC-NSCs), neurons (hiPSC-Neurons), and microglia-like cells (hiPSC-MGLs) from sporadic AD (sAD) patients, age-matched cognitive normal controls (CNCs), and young subjects to observe human neural cell-type response to H2O2 stimulation. Without H2O2 exposure, reactive oxygen species (ROS) cannot be detected in hiPSC-NSCs from all three groups, but the viability of hiPSC-NSCs from AD patients was significantly lower than those of CNCs and young subjects. There were no significant differences in ROS, viabilities, neurite length, and neurite branch points in hiPSC-Neurons among three groups. No significant differences in viabilities, phagocytosis, and secretion of cytokines were observed in hiPSC-MGLs among three groups, but higher ROS levels in sAD hiPSC-MGLs. Under H2O2 exposure, the viability, neurite length, and neurite branch points of hiPSC-Neurons from AD patients reduced more significantly accompanied by more ROS release. H2O2 exposure caused hiPSC-MGLs from AD patients to release more ROS, cytokines, and stronger phagocytosis. Nevertheless, H2O2 exposure had no effect on viability of hiPSC-NSCs. Our results showed hiPSC-Neurons and hiPSC-MGLs were more sensitive to H2O2 than hiPSC-NSCs, which indicated the different response styles of hiPSC-NSCs, hiPSC-Neurons, and hiPSC-MGLs to oxidative stress. HiPSC-derived neural cells from AD patients suffered more severe injury from H2O2 than those of CNCs and young subjects, indicating that the vulnerability to oxidative stress of AD patients can be recapitulated in hiPSCs.
- Published
- 2020
41. PRMT5 regulates RNA m6A demethylation for doxorubicin sensitivity in breast cancer
- Author
-
Ying Wu, Zhe Wang, Lu Han, Zhihao Guo, Bohua Yan, Lili Guo, Huadong Zhao, Mengying Wei, Niuniu Hou, Jing Ye, Changhong Shi, Suling Liu, Ceshi Chen, Suning Chen, Ting Wang, Jun Yi, JianPing Zhou, Libo Yao, Wenxia Zhou, Rui Ling, and Jian Zhang
- Subjects
Pharmacology ,Protein-Arginine N-Methyltransferases ,Breast Neoplasms ,Demethylation ,Tadalafil ,Doxorubicin ,Drug Discovery ,Genetics ,Molecular Medicine ,Humans ,RNA ,Original Article ,Female ,Molecular Biology - Abstract
Cancer cells respond to various stressful conditions through the dynamic regulation of RNA m6A modification. Doxorubicin is a widely used chemotherapeutic drug that induces DNA damage. It is interesting to know whether cancer cells regulate the DNA damage response and doxorubicin sensitivity through RNA m6A modification. Here, we found that doxorubicin treatment significantly induced RNA m6A methylation in breast cancer cells in both a dose- and a time-dependent manner. However, protein arginine methyltransferase 5 (PRMT5) inhibited RNA m6A modification under doxorubicin treatment by enhancing the nuclear translocation of the RNA demethylase AlkB homolog 5 (ALKBH5), which was previously believed to be exclusively localized in the nucleus. Then, ALKBH5 removed the m6A methylation of BRCA1 for mRNA stabilization and further enhanced DNA repair competency to decrease doxorubicin efficacy in breast cancer cells. Importantly, we identified the approved drug tadalafil as a novel PRMT5 inhibitor that could decrease RNA m6A methylation and increase doxorubicin sensitivity in breast cancer. The strategy of targeting PRMT5 with tadalafil is a promising approach to promote breast cancer sensitivity to doxorubicin through RNA methylation regulation.
- Published
- 2022
42. Synthesis and properties of an Au6 cluster supported by a mixed N-heterocyclic carbene–thiolate ligand
- Author
-
Masakazu Nambo, Maryam Sabooni Asre Hazer, Hannu Häkkinen, Alex Veinot, Paul A. Lummis, Renee W. Y. Man, Wenxia Zhou, Cathleen M. Crudden, Kirsi Salorinne, Sami Malola, and Jacky C.-H. Yim
- Subjects
Benzimidazole ,Denticity ,010405 organic chemistry ,Ligand ,Metals and Alloys ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Crystallography ,chemistry ,Chain (algebraic topology) ,Materials Chemistry ,Ceramics and Composites ,Cluster (physics) ,Density functional theory ,Carbene ,Linker - Abstract
The preparation of a novel Au6 cluster bearing a bidentate mixed carbene–thiolate ligand is presented. The length of linker between the central benzimidazole and thiolate has a strong effect on the formation of cluster products, with a C2 chain giving an Au6 cluster, while a C3 chain results in no evidence of cluster formation. Density functional theory analysis predicts a non-metallic cluster with a large HOMO–LUMO (3.2–3.6 eV) and optical gap.
- Published
- 2020
43. Improving the Environmental Footprint through Employees: A Case of Female Leaders from the Perspective of CSR
- Author
-
Shilong Wei, Muhammad Safdar Sial, Wenxia Zhou, Alina Badulescu, and Daniel Badulescu
- Subjects
Male ,Organizations ,Social Responsibility ,pro-environmental behavior ,healthcare sector ,Health, Toxicology and Mutagenesis ,CSR ,ethical leadership ,gender ,Public Health, Environmental and Occupational Health ,Morals ,Article ,Leadership ,Surveys and Questionnaires ,Medicine ,Humans ,Female - Abstract
Environmental quality strongly depends on human behavior patterns. Many environmental challenges are rooted in human actions, and thus, it is believed that these problems can be reduced through the promotion of pro-environmental behaviors (PB). Owing to this reality, the current study aims to reduce the environmental footprint of a hospital by promoting its employees’ environment-specific behavior via corporate social responsibility (CSR) and ethical leadership (EL). More importantly, the study also considered the role of female leaders in the proposed relationship. The current study collected the data from the respondents employed in different hospitals of a developing economy through a questionnaire (paper-pencil method). A total of 489 valid responses were collected, which were analyzed by employing the structural equation modeling (SEM) technique. As per the current study’s findings, there is a positive relationship between CSR, while EL mediates between CSR and PB. Likewise, the moderating role of female leaders in the proposed relationship was more significant than that of male leaders. More specifically, the study’s findings have considerable theoretical and practical implications, as it opens paths for researchers to further investigate the applicability of different dimensions of CSR and the role of gender in environmental sustainability. It provides insight to policymakers on how to restructure their CSR preferences, priorities on the environment, and gender differences.
- Published
- 2021
44. Comprehensive analysis of expression profiles and prognosis of TRIM genes in human kidney clear cell carcinoma
- Author
-
Junwen Shen, Rongjiang Wang, Yu Chen, Zhihai Fang, Jianer Tang, Jianxiang Yao, Jianguo Gao, Wenxia Zhou, and Xiongnong Chen
- Subjects
Tripartite Motif Proteins ,Aging ,MicroRNAs ,Ubiquitin-Protein Ligases ,Humans ,Cell Biology ,Prognosis ,Carcinoma, Renal Cell ,Kidney Neoplasms - Abstract
To determine survival rates and the underlying mechanism of genes in the TRIM family in Kidney Clear Cell Carcinoma (KIRC).Transcriptional and survival data of TRIM genes in KIRC patients were retrieved from the UCSC Xena, and GEPIA databases. The function of TRIM genes in KIRC was investigated, focusing on potential ubiquitination, miRNAs regulation, and enrichment analysis. Next, TRIM gene survival values were determined, followed by the development of a survival-related signature.Only TRIM26 was down expressed in the carcinoma tissue and had a survival value in KIRC relative to control tissues, which was supplied by vitro experiment. The patients with lower expression of TRIM26 would have the chance to live a shorter time. SNRPB, which also plays a role in ubiquitination, directly interacted with TRIM26. Moreover, two miRNAs (hsa-let-7i-5p, and hsa-miR-1228-5p) that regulated levels of TRIM26 expression were also identified. Next, we constructed a signature (TRIM4/7/27/58/65/72) and found that high-risk scores of the signature were associated with poor survival rates in KIRC patients. while its resultant risk scores were correlated with immune cell components and markers.TRIM26 was differentially expressed between KIRC and normal tissues and had a survival value in the KIRC. hsa-let-7i-5p/hsa-miR-1228-5p-TRIM26-SNRPB was a potential mechanism axis that may play a role on the KIRC cells. A survival signature (TRIM4/7/27/58/65/72) was successfully established to predict the survival of KIRC patients.
- Published
- 2021
45. Good or Bad? The Ambivalent Leader-Follower Relationships
- Author
-
Wenxia Zhou and Qinglin Zhao
- Subjects
Integrative complexity ,Perspective (graphical) ,Stressor ,Workplace relationships ,Flexibility (personality) ,workplace relationships ,Conservation of resources theory ,Ambivalence ,BF1-990 ,conservation of resources theory ,Perspective ,Psychology ,stressor ,Disengagement theory ,paradox view ,ambivalent relationships ,Social psychology ,General Psychology - Abstract
Researchers have emphasized the positive and negative influences of ambivalent leader-follower relationships, but it is not clear when the ambivalent relationship is associated with good or bad influences. To answer this question, we reviewed the definition and identified 10 different types of ambivalent leader-follower relationships. Further, we demonstrate that the negative outcomes (more inflexibility, disengagement, and worse performance) can be explained by the workplace stressor perspective, and that the positive outcomes (more flexibility, engagement, and better performance) can be explained by paradox view. Finally, drawing from conservation of resources (COR) theory, we integrate workplace stressor framework and the paradox view to address when the ambivalent leader-follower relationship is beneficial or detrimental for followers. We proposed that the degree of ambivalence, support from the third party, and integrative complexity of follower will influence the possible positive or negative influences. Limitations and future directions were also discussed.
- Published
- 2021
46. When Does Future Work Self Predict Work Engagement: The Boundary Conditions of Person-Vocation Fit and Trust in Supervisor
- Author
-
Ying Xu, Wenxia Zhou, and Ping Guo
- Subjects
Psychiatry and Mental health ,Supervisor ,Work (electrical) ,Health Policy ,Work engagement ,Public Health, Environmental and Occupational Health ,Boundary value problem ,Psychology ,Social psychology ,Mental health - Published
- 2019
47. How Does Proactive Personality Promote Affective Well-Being? A Chained Mediation Model
- Author
-
Wenxia Zhou, Mengyi Li, Wenxi Wang, Fangcheng Tang, and Lu Xin
- Subjects
Psychiatry and Mental health ,Health Policy ,media_common.quotation_subject ,Mediation ,Well-being ,Public Health, Environmental and Occupational Health ,Personality ,Psychology ,Mental health ,Social psychology ,media_common - Published
- 2019
48. Analysis and verification of fluorescence super-resolution microscopy via polarization modulation in reciprocal space
- Author
-
Jianhua Yin, Xiao Wang, Dongdong Xu, and Wenxia Zhou
- Subjects
Physics ,business.industry ,Super-resolution microscopy ,Resolution (electron density) ,Reconstruction algorithm ,Polarization (waves) ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,010309 optics ,Reciprocal lattice ,Optics ,0103 physical sciences ,Microscopy ,Fluorescence microscope ,Computer Vision and Pattern Recognition ,Spatial frequency ,business - Abstract
Based on the polarization property of fluorescent dipoles, fluorescence super-resolution microscopy recently has been proposed by modulating the polarization of the excitation light. In this technique, the super-resolution image is reconstructed by processing the polarization-modulated fluorescence image stack with an iteration algorithm. However, the mechanism of resolution improvement by polarization modulation has been questioned. In this paper, the mechanism of resolution enhancement by polarization modulation is analyzed in reciprocal space. The mathematical model and the reconstruction algorithm of fluorescence super-resolution microscopy via polarization modulation are proposed in reciprocal space. The corresponding simulation results and analysis show that polarization modulation can enlarge the highest detected spatial frequency of fluorescence microscopy to achieve super resolution, which verifies the role of polarization modulation in resolution improvement and provides a useful reference to study fluorescence super-resolution microscopy via polarization modulation in reciprocal space.
- Published
- 2021
49. Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype
- Author
-
Kang Xiahou, Xiaohui Liu, Yongxiang Zhang, Wenxia Zhou, Qiao Ren, Gang Liu, Shulin Meng, Ning Jiang, and Lin Zhang
- Subjects
0301 basic medicine ,Homeobox protein NANOG ,Genotype ,QH301-705.5 ,Cellular differentiation ,Induced Pluripotent Stem Cells ,Apolipoprotein E3 ,Embryoid body ,Biology ,Peripheral blood mononuclear cell ,Flow cytometry ,03 medical and health sciences ,Kruppel-Like Factor 4 ,0302 clinical medicine ,SOX2 ,Alzheimer Disease ,medicine ,Humans ,Biology (General) ,Induced pluripotent stem cell ,Aged, 80 and over ,medicine.diagnostic_test ,Cell Differentiation ,Cell Biology ,General Medicine ,030104 developmental biology ,KLF4 ,embryonic structures ,Cancer research ,Leukocytes, Mononuclear ,Female ,biological phenomena, cell phenomena, and immunity ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis.
- Published
- 2021
50. High-performance work systems and key employee attitudes: the roles of psychological capital and an interactional justice climate
- Author
-
Rentao Miao, Nikolaos Bozionelos, Wenxia Zhou, and Alexander Newman
- Subjects
Organizational citizenship behavior ,Organizational Behavior and Human Resource Management ,Strategy and Management ,05 social sciences ,050209 industrial relations ,N600 ,Procedural justice ,Business economics ,Social exchange theory ,Management of Technology and Innovation ,Interactional justice ,Capital (economics) ,Human resource management ,0502 economics and business ,Industrial relations ,N100 ,Business and International Management ,Work systems ,Psychology ,Social psychology - Abstract
While there is evidence that high performance work systems (HPWS) are related to positive employee attitudes, the underlying mechanism is not yet thoroughly understood. Similarly, though the benefits of employees’ psychological capital (PsyCap) in the workplace are well documented, little is known about the extent to which PsyCap stems from the context in which people work. Adopting a multi-level approach and using the ability-motivation-opportunity (AMO) framework and the conservation of resources (COR) theory as theoretical backdrop, we develop and test a moderated mediation model to explain how organization-level HPWS relate to individual employee work attitudes (job satisfaction and affective organizational commitment) that reflect well-being through the mediating mechanism of employees’ PsyCap. Analysis of multi-source data from 569 employees in 44 firms showed that HPWS were positively related to job satisfaction and affective commitment, and that these relationships were partially mediated by PsyCap. Furthermore, moderated path analysis revealed that an interactional justice climate strengthened the direct effect of HPWS on PsyCap and work attitudes as well as the indirect effects of HPWS on work attitudes. The theoretical and practical implications of these findings are discussed.
- Published
- 2021
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