383 results on '"Weipeng, Wang"'
Search Results
2. Disruption of LPA‐LPAR1 pathway results in lung tumor growth inhibition by downregulating B7‐H3 expression in fibroblasts
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Fanyi Meng, Zhiyue Yin, Feifei Lu, Weipeng Wang, and Hongjian Zhang
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B7‐H3 ,cancer‐associated fibroblasts ,LPAR1 ,lysophosphatidic acid ,non‐small cell lung cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Lysophosphatidic acids (LPAs) belong to a class of bioactive lysophospholipids with multiple functions including immunomodulatory roles in tumor microenvironment (TME). LPA exerts its biological effects via its receptors that are highly expressed in fibroblasts among other cell types. As cancer‐associated fibroblasts (CAFs) are a key component of the TME, it is important to understand LPA signaling and regulation of receptors in fibroblasts or CAFs and associated regulatory roles on immunomodulation‐related molecules. Methods Cluster analysis, immunoblotting, real‐time quantitative‐PCR, CRISPR‐Cas9 gene editing system, immunohistochemical staining, coculture model, and in vivo xenograft model were used to investigate the effects of LPA‐LPAR1 on B7‐H3 in tumor promotion of CAFs. Results In this study, we found that LPAR1 and CD276 (B7‐H3) were generally highly expressed in fibroblasts with good expression correlation. LPA induced B7‐H3 up‐expression through LPAR1, and stimulated fibroblasts proliferation that could be inhibited by silencing LPAR1 or B7‐H3 as well as small molecule LPAR1 antagonist (Ki16425). Using engineered fibroblasts and non‐small cell lung carcinoma (NSCLC) cell lines, subsequent investigations demonstrated that CAFs promoted the proliferation of NSCLC in vitro and in vivo, and such effect could be inhibited by knocking out LPAR1 or B7‐H3. Conclusion The present study provided new insights for roles of LPA in CAFs, which could lead to the development of innovative therapies targeting CAFs in the TME. It is also reasonable to postulate a combinatory approach to treat malignant fibrous tumors (such as NSCLC) with LPAR1 antagonists and B7‐H3 targeting therapies.
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- 2024
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3. PG² Net: Personalized and Group Preferences Guided Network for Next Place Prediction.
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Bin Wang 0052, Huifeng Li, Weipeng Wang, Menghan Wang, Yaohui Jin, and Yanyan Xu 0002
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- 2024
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4. 8K Real-Time Video-Transmission Using PDM and FDM in 220-GHz Band Based on Polarization Crosstalk Model and Optimization Strategy.
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Haoyi Cao, Zheng Wang, Weipeng Wang, Zhan Zhang, Wenbo Li, Tianchi Zhou, Hongji Zhou, Yazhou Dong, Hongqiang Wang 0001, Qi Yang 0002, Xun Wang, Qiang Xu, Ziqiang Yang, Hongxin Zeng, Lin Huang, and Yaxin Zhang
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- 2024
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5. Antibacterial peptide Reg4 ameliorates Pseudomonas aeruginosa-induced pulmonary inflammation and fibrosis
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Xiaoyu Wan, Weipeng Wang, Jing Zhu, and Yongtao Xiao
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Pseudomonas aeruginosa ,pulmonary fibrosis ,Reg4 ,antimicrobial peptides ,macrophage ,Microbiology ,QR1-502 - Abstract
ABSTRACTPseudomonas aeruginosa (P. aeruginosa) is a Gram-negative facultative anaerobe that has become an important cause of severe infections in humans, particularly in patients with cystic fibrosis. The development of efficacious methods or mendicants against P. aeruginosa is still needed. We previously reported that regenerating islet-derived family member 4 (Reg4) has bactericidal activity against Salmonella Typhimurium, a Gram-negative flagellated bacterium. We herein explore whether Reg4 has bactericidal activity against P. aeruginosa. In the P. aeruginosa PAO1-chronic infection model, Reg4 significantly inhibits the colonization of PAO1 in the lung and subsequently ameliorates pulmonary inflammation and fibrosis. Reg4 recombinant protein suppresses the growth motility and biofilm formation capability of PAO1 in vitro. Mechanistically, Reg4 not only exerts bactericidal action via direct binding to the P. aeruginosa cell wall but also enhances the phagocytosis of alveolar macrophages in the host. Taken together, our study demonstrates that Reg4 may provide protection against P. aeruginosa-induced pulmonary inflammation and fibrosis via its antibacterial activity.IMPORTANCEChronic lung infection with Pseudomonas aeruginosa is a leading cause of morbidity and mortality in patients with cystic fibrosis. Due to the antibiotic resistance of Pseudomonas aeruginosa, antimicrobial peptides appear to be a potential alternative to combat its infection. In this study, we report an antimicrobial peptide, regenerating islet-derived 4 (Reg4), that showed killing activity against clinical strains of Pseudomonas aeruginosa PAO1 and ameliorated PAO1-induced pulmonary inflammation and fibrosis. Experimental data also showed Reg4 directly bound to the bacterial cell membrane and enhanced the phagocytosis of host alveolar macrophages. Our presented study will be a helpful resource in searching for novel antimicrobial peptides that could have the potential to replace conventional antibiotics.
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- 2024
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6. Loss of Mptx2 alters bacteria composition and intestinal homeostasis potentially by impairing autophagy
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Weihui Yan, Shanshan Chen, Ying Wang, Yaying You, Ying Lu, Weipeng Wang, Bo Wu, Jun Du, Shicheng Peng, Wei Cai, and Yongtao Xiao
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Biology (General) ,QH301-705.5 - Abstract
Abstract A recent single-cell survey of the small-intestinal epithelium suggests that mucosal pentraxin 2 (Mptx2) is a new Paneth cell marker, but its function and involved mechanism in the Paneth cell are still unknown. Therefore, we create Mptx2 knockout (Mptx2 −/− ) mice to investigate its precise effects on intestinal homeostasis using models of lipopolysaccharide (LPS), methicillin-resistant Staphylococcus aureus (MRSA) peritoneal infection, and dextran sulfate sodium (DSS)–induced intestinal injury and inflammation. We here find that Mptx2 is selectively expressed in Paneth cells in the small intestines of mice. Mptx2 −/− mice have increased susceptibility to intestinal inflammation and injured. Mptx2 deficiency reduces Paneth cell count and expression of antimicrobial factors, leading to altered intestinal bacteria composition. Loss of Mptx2 aggravates MRSA infection–induced damage in the intestine while decreasing autophagy in Paneth cells. Mptx2 −/− mice are more vulnerable to LPS-induced intestinal possibly due to inhibition of the autophagy/endoplasmic reticulum (ER) stress pathway. Mptx2 −/− mice are susceptible to DSS-induced colitis that could be ameliorated by treatment with gentamicin or vancomycin antibiotics. In conclusion, Mptx2 is essential to maintain intestinal homeostasis potentially via regulation of autophagy in Paneth cells.
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- 2024
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7. Microwave-induced thermoacoustic elastic imaging: A simulation study
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Lin Huang, Zheng Liang, Shuaiqi Qiao, and Weipeng Wang
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Thermoacoustic imaging ,breast cancer ,multi-physics simulation ,elastic imaging ,Technology ,Optics. Light ,QC350-467 - Abstract
Microwave-induced thermoacoustic imaging (MTI) has the advantages of high resolution, high contrast, non-ionization, and non-invasive. Recently, MTI was used in the field of breast cancer screening. In this paper, based on the finite element method (FEM) and COMSOL Multiphysics software, a three-dimensional breast cancer model suitable for exploring the MTI process is proposed to investigate the influence of Young’s modulus (YM) of breast cancer tissue on MTI. It is found that the process of electromagnetic heating and initial pressure generation of the entire breast tissue is earlier in time than the thermal expansion process. Besides, compared with normal breast tissue, tumor tissue has a greater temperature rise, displacement, and pressure rise. In particular, YM of the tumor is related to the speed of thermal expansion. In particular, the larger the YM of the tumor is, the higher the heating and contraction frequency is, and the greater the maximum pressure is. Different Young’s moduli correspond to different thermoacoustic signal spectra. In MTI, this study can be used to judge different degrees of breast cancer based on elastic imaging. In addition, this study is helpful in exploring the possibility of microwave-induced thermoacoustic elastic imaging (MTAE).
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- 2024
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8. Experience in the treatment of long-gap esophageal atresia by intraluminal esophageal stretching elongation
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Ning Zhang, Wenjie Wu, Yujia Zhuang, Weipeng Wang, Weihua Pan, and Jun Wang
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esophageal atresia ,long-gap ,intraluminal esophageal stretching ,elongation ,postoperative complications ,Pediatrics ,RJ1-570 - Abstract
ObjectiveTo summarize the experience with intraluminal esophageal stretching elongation (ILESE) in the successful treatment of long-gap esophageal atresia (LGEA) at a single center.MethodsClinical data of 68 neonates who underwent LGEA between February 2015 and January 2022 were retrospectively analyzed. Four patients died of multiple associated severe malformations and did not undergo ILESE. Esophageal anastomosis was successfully performed in 60 cases (93.75%) and failed in 4 cases (6.25%) treated with ILESE. The ILESE techniques, esophageal reconstruction, results, postoperative complications, and follow-up treatment were analyzed.ResultsThe beginning time of performing ILESE preoperation was 53.4 ± 39.4 days after birth, and the age of esophageal reconstruction was 122.2 ± 70.3 days after birth in 60 cases. The gap length of proximal and distal esophageal segments which were evaluated the first time at admission was 4.8 ± 1.3 vertebral bodies, whereas the gap before anastomosis was −0.46 ± 0.90 vertebral bodies. Among the patients with esophageal primary-anastomosis, 55 received thoracoscopic surgery, and 5 underwent thoracotomy in the early stage. Of the 60 children with ILESE, 58 underwent end-to-end esophagostomy, of which 17 cases were combined with circular esophagotomy (livaditis), and 2 cases of esophageal lengthening were combined with the reversal of the ligulate loop of the proximal esophagus (flap). Overall, 59 cases were cured (98.3%), and 1 patient died of respiratory failure postoperatively. All patients were followed up for 7–96 months. Postoperative anastomotic leakage occurred in 16 patients (27.6%), all of whom were successfully treated conservatively. Anastomotic stenosis occurred in 49 cases (83.1%), all of which were successfully managed by non-surgical treatment, including 12.7 ± 9.3 times of esophageal balloon dilatation and 2 cases of stent dilatation. Gastroesophageal reflux occurred in 44 patients (74.6%), including associated or acquired esophageal hiatal hernia in 22 patients, and Nissen fundoplication was performed in 17 patients.ConclusionsILESE is an effective method for prolonging the proximal and distal esophagus of the LGEA to reconstruct esophageal continuity using its esophageal tissue, with an efficacy rate of 93.75%. Postoperative anastomotic stricture and gastroesophageal reflux are common and require long-term, standardized follow-up and treatment.
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- 2024
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9. FDW028, a novel FUT8 inhibitor, impels lysosomal proteolysis of B7-H3 via chaperone-mediated autophagy pathway and exhibits potent efficacy against metastatic colorectal cancer
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Mengmeng Wang, Zhoudong Zhang, Mengxi Chen, Yixin Lv, Sheng Tian, Fanyi Meng, Yawen Zhang, Xuqin Guo, Yinshuang Chen, Man Yang, Jiawei Li, Tian Qiu, Fang Xu, Zhi Li, Qi Zhang, Jie Yang, Jing Sun, Hongjian Zhang, Haiyang Zhang, Huanqiu Li, and Weipeng Wang
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Cytology ,QH573-671 - Abstract
Abstract Metastatic colorectal cancer (mCRC) is a major cause of cancer-related mortality due to the absence of effective therapeutics. Thus, it is urgent to discover new drugs for mCRC. Fucosyltransferase 8 (FUT8) is a potential therapeutic target with high level in most malignant cancers including CRC. FUT8 mediates the core fucosylation of CD276 (B7-H3), a key immune checkpoint molecule (ICM), in CRC. FUT8-silence-induced defucosylation at N104 on B7-H3 attracts heat shock protein family A member 8 (HSPA8, also known as HSC70) to bind with 106-110 SLRLQ motif and consequently propels lysosomal proteolysis of B7-H3 through the chaperone-mediated autophagy (CMA) pathway. Then we report the development and characterization of a potent and highly selective small-molecule inhibitor of FUT8, named FDW028, which evidently prolongs the survival of mice with CRC pulmonary metastases (CRPM). FDW028 exhibits potent anti-tumor activity by defucosylation and impelling lysosomal degradation of B7-H3 through the CMA pathway. Taken together, FUT8 inhibition destabilizes B7-H3 through CMA-mediated lysosomal proteolysis, and FDW028 acts as a potent therapeutic candidate against mCRC by targeting FUT8. FDW028, an inhibitor specifically targeted FUT8, promotes defucosylation and consequent HSC70/LAMP2A-mediated lysosomal degradation of B7-H3, and exhibits potent anti-mCRC activities.
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- 2023
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10. Study of Correlation between Fetal Bowel Dilation and Congenital Gastrointestinal Malformation
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Yi Jiang, Weipeng Wang, Weihua Pan, Wenjie Wu, Dan Zhu, and Jun Wang
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fetus ,bowel dilation ,congenital gastrointestinal malformation ,Pediatrics ,RJ1-570 - Abstract
Background: Ultrasound serves as a valuable tool for the early detection of fetal bowel dilatation, yet the correlation between fetal bowel dilatation and gastrointestinal malformations remains to be further investigated. This study aims to explore the relationship by conducting a follow-up and analysis of fetuses with bowel dilation. Methods: A retrospective analysis was conducted on 113 fetuses with bowel dilatation at our center from July 2014 to December 2019. The location and degree of bowel dilatation were analyzed. ROC curves were constructed based on the diameter of the bowel and its ratio to fetal gestational age. Results: In total, 40 of 41 cases (97.6%) with upper gastrointestinal dilatation (double-bubble sign) and 46 of 72 cases (63.9%) with lower gastrointestinal dilatation were diagnosed with gastrointestinal malformations postnatally. The AUC of the dilatation diameter was 0.854 with a cutoff value of 18.05 mm in patients with lower gastrointestinal dilatation. The ratio of the diameter to gestational age (D/GA) showed a higher AUC of 0.906 with a cutoff value of 0.4931. Conclusions: The presence of the double-bubble sign in fetuses indicates a close association with duodenal obstruction. The risk of gastrointestinal malformations increases when the bowel diameter exceeds 18.05 mm, particularly when the D/GA surpasses 0.4931.
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- 2024
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11. When Configuration Verification Meets Machine Learning: A DRL Approach for Finding Minimum k-Link Failures.
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Hao Chen, Yili Jin, Weipeng Wang, Wei Liu, Lizhao You, Liqun Fu 0001, and Qiao Xiang
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- 2023
12. Deep-Unfolding Aided Hybrid Precoding Design Based on Penalty Dual Decomposition for Full-Duplex mmWave System.
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Anqi Xue, Weipeng Wang, and Liqun Fu 0001
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- 2023
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13. PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression
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Yuling Sun, Jie Yang, Yachun Chen, Yundi Guo, Jian Xiong, Xuqin Guo, Yawen Zhang, Li Gu, Min Tong, Weipeng Wang, and Jing Sun
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Background. This work focused on investigating the role of programmed death ligand 2 (PD-L2) in the progression of breast cancer by utilizing breast cancer specimens and cells. Materials and Methods. The serum levels of soluble PD-L2 (sPD-L2) in breast cancer patients and healthy individuals were analyzed by means of the enzyme-linked immunosorbent assay, and the PD-L2 levels within 416 resected breast cancer specimens were assessed through immunohistochemistry. Concurrently, in vitro cell experiments and in vivo animal experiments were carried out to analyze the relationship between PD-L2 and the invasion and migration of breast cancer. Results. The concentration of sPD-L2 in breast cancer patients significantly increased compared to that in the control groups. Additionally, breast cancer patients with high concentrations of sPD-L2 had higher Ki67 values (≥30%) and tumor grades. PD-L2 was expressed in 79.09% of the cancer samples, which exhibited a positive correlation with the progesterone receptor (PR) and the human epidermal growth factor receptor 2 (HER2). Furthermore, we discovered that knockdown of PD-L2 inhibited the migratory and invasive abilities of both MCF-7 and MDA-MB231 cells. Conclusion. Our findings demonstrated that knockdown of PD-L2 suppressed tumor growth, providing novel insights into important biological functions.
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- 2024
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14. Transcriptome profile analysis revealed the potential mechanism of LIPUS treatment for Adriamycin-induced chronic kidney disease rat
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Zhiqiang Ouyang, Guodong Zhang, Weipeng Wang, Lishi Shao, Xiaolan Du, Guocheng Li, Na Tan, Xinyan Zhou, Jun Yang, Lin Huang, and Chengde Liao
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Chronic kidney disease ,low intensity pulsed ultrasound ,Transcriptomics ,Nuclear factor kappa B ,Ferroptosis ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Developing effective therapeutic strategies to delay the progression of chronic kidney disease (CKD) remains a significant challenge. Low-intensity pulsed ultrasound (LIPUS) has demonstrated potential for treating CKD, but the underlying molecular mechanisms are still elusive. This study aimed to evaluate the therapeutic efficacy of LIPUS and to elucidate the involved genes and signaling pathways. Methods: The CKD model was established in rats using Adriamycin (ADR). The bilateral kidneys of CKD rats were continuously stimulated with LIPUS for a period of four weeks. The therapeutic efficacy was defined by renal function and histopathological evaluation. RNA sequencing was employed to profile the transcriptome of rat kidneys in each group. Cluster analysis was utilized to identify differentially expressed genes (DEGs), followed by enrichment analysis of their associated pathways using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results: LIPUS treatment improved ADR-induced renal dysfunction in the CKD group. Renal fibrosis and pathological damages were also alleviated in the ADR + LIPUS group compared to the ADR group. Cluster analysis identified 844 DEGs. GO enrichment analysis revealed enrichment in inflammatory response terms, while KEGG enrichment analysis highlighted the nuclear factor kappa B (NF-κB) signaling and ferroptosis-related pathways. Conclusion: Continuous LIPUS treatment improved ADR-induced renal fibrosis and dysfunction. The therapeutic effect of LIPUS was primarily due to its ability to suppress the CKD-related inflammation, which was associated with the modulation of the NF-κB and ferroptosis signaling pathways. These findings provide a new insight into the potential molecular mechanisms of LIPUS in treating CKD. Further research is necessary to confirm these findings and to identify potential therapeutic targets within these pathways.
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- 2023
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15. Thickness dependence of the crystallization and phase transition in ZrO2 thin films
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Yue Guan, Jing Zhou, Haodong Zhong, Weipeng Wang, Zhengjun Zhang, Feng Luo, and Shuai Ning
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zirconia ,crystallization ,tetragonal (t) ,monoclinic (m) ,phase transition ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
Fluorite-structure binary oxides (e.g., HfO2 and ZrO2) have attracted increasing interest for a broad range of applications including thermal barrier coatings, high-k dielectrics, and novel ferroelectrics. A crystalline structure plays a crucial role in determining physical and chemical properties. Structure evolution of ZrO2 thin films, particularly down to the nanometer scale, has not been thoroughly studied. In this work, we carried out systematic annealing analysis on the ZrO2 thin films. Through in-situ high-temperature X-ray diffraction (XRD) characterizations, a thickness dependence of crystallization and phase transition is observed. Irrespective of the thickness (10–300 nm), the as-prepared amorphous ZrO2 thin films are preferentially crystallized into a tetragonal (t) structure (high-temperature phase), which can be preserved down to room temperature (RT) upon annealing at the corresponding crystallization temperature (TC). When annealing at temperatures higher than TC, the transition from t to monoclinic (m; RT phase) will occur, and the quantity of the transition strongly depends on the film thickness. Our work expands the basic understanding of the phase transition in the ZrO2 thin films, and offers a path to the selective control over the phase structure for novel functionalities.
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- 2023
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16. Inositol hexaphosphate promotes intestinal adaptation in short bowel syndrome via an HDAC3-mediated epigenetic pathway
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Weipeng Wang, Ying Wang, Ying Lu, Xinbei Tian, Shanshan Chen, Bo Wu, Jun Du, Yongtao Xiao, and Wei Cai
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inositol hexaphosphate ,short bowel syndrome ,intestinal adaptation ,hdac3 ,cell proliferation ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: Short bowel syndrome (SBS) has high morbidity and mortality rates, and promoting intestinal adaptation of the residual intestine is a critical treatment. Dietary inositol hexaphosphate (IP6) plays an important role in maintaining intestinal homeostasis, but its effect on SBS remains unclear. This study aimed at investigating the effect of IP6 on SBS and clarified its underlying mechanism. Methods: Forty male Sprague–Dawley rats (3-week-old) were randomly assigned into four groups (Sham, Sham + IP6, SBS, and SBS + IP6 groups). Rats were fed standard pelleted rat chow and underwent resection of 75% of the small intestine after 1 week of acclimation. They received 1 mL IP6 treatment (2 mg/g) or sterile water daily for 13 days by gavage. Intestinal length, levels of inositol 1,4,5-trisphosphate (IP3), histone deacetylase 3 (HDAC3) activity, and proliferation of intestinal epithelial cell-6 (IEC-6) were detected. Results: IP6 treatment increased the length of the residual intestine in rats with SBS. Furthermore, IP6 treatment caused an increase in body weight, intestinal mucosal weight, and IEC proliferation, and a decrease in intestinal permeability. IP6 treatment led to higher levels of IP3 in feces and serum, and higher HDAC3 activity of the intestine. Interestingly, HDAC3 activity was positively correlated with the levels of IP3 in feces (r = 0.49, P = 0.01) and serum (r = 0.44, P = 0.03). Consistently, IP3 treatment promoted the proliferation of IEC-6 cells by increasing HDAC3 activity in vitro. IP3 regulated the Forkhead box O3 (FOXO3)/Cyclin D1 (CCND1) signaling pathway. Conclusion: IP6 treatment promotes intestinal adaptation in rats with SBS. IP6 is metabolized to IP3 to increase HDAC3 activity to regulate the FOXO3/CCND1 signaling pathway and may represent a potential therapeutic approach for patients with SBS.
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- 2023
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17. CPS: Full-Song and Style-Conditioned Music Generation with Linear Transformer.
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Weipeng Wang, Xiaobing Li, Cong Jin, Di Lu, Qingwen Zhou, and Yun Tie
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- 2022
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18. Ultrahigh resistance of hexagonal boron nitride to mineral scale formation
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Kuichang Zuo, Xiang Zhang, Xiaochuan Huang, Eliezer F. Oliveira, Hua Guo, Tianshu Zhai, Weipeng Wang, Pedro J. J. Alvarez, Menachem Elimelech, Pulickel M. Ajayan, Jun Lou, and Qilin Li
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Science - Abstract
Scale formation may have detrimental effects on the properties and functions of materials’ surfaces. Here the authors report the high scaling resistance of hexagonal boron nitride and relate it to the atomic level structure and interaction with water molecules.
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- 2022
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19. A new gain-of-function OsGS2/GRF4 allele generated by CRISPR/Cas9 genome editing increases rice grain size and yield
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Wenshu Wang, Weipeng Wang, Yanlin Pan, Chao Tan, Hongjing Li, Ya Chen, Xingdan Liu, Jing Wei, Nian Xu, Yu Han, Han Gu, Rongjian Ye, Qi Ding, and Chonglie Ma
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Genome editing ,GS2/GRF4 ,Grain size ,Yield ,Rice ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Grain size is one of the most important factors affecting rice grain quality and yield, and attracts great attention from molecular biologists and breeders. In this study, we engineered a CRISPR/Cas9 system targeting the miR396 recognition site of the rice GS2 gene, which encodes growth-regulating factor 4 (OsGRF4) and regulates multiple agronomic traits including grain size, grain quality, nitrogen use efficiency, abiotic stress response, and seed shattering. In contrast to most previous genome editing efforts in which indel mutations were chosen to obtain null mutants, a mutant named GS2E carrying an in-frame 6-bp deletion and 1-bp substitution within the miR396-targeted sequence was identified. GS2E plants showed increased expression of GS2 in consistent with impaired repression by miR396. As expected, the gain-of-function GS2E mutant exhibited multiple beneficial traits including increased grain size and yield and bigger grain length/width ratio. Thousand grain weight and grain yield per plant of GS2E plants were increased by 23.5% and 10.4%, respectively. These improved traits were passed to hybrids in a semi-dominant way, suggesting that the new GS2E allele has great potential in rice improvement. Taken together, we report new GS2 germplasm and describe a novel gene-editing strategy that can be widely employed to improve grain size and yield in rice. This trait-improvement strategy could be applied to other genes containing miRNA target sites, in particular the conserved miR396-GRF/GIF module that governs plant growth, development and environmental response.
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- 2022
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20. Obesity, non-alcoholic fatty liver disease and hepatocellular carcinoma: current status and therapeutic targets
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Yinshuang Chen, Weipeng Wang, Maria P. Morgan, Tracy Robson, and Stephanie Annett
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hepatocellular carcinoma (HCC) ,obesity ,nonalcoholic fatty liver disease (NAFLD) ,nonalcoholic steatohepatitis (NASH) ,therapeutic targets ,animal models ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Obesity is a global epidemic and overwhelming evidence indicates that it is a risk factor for numerous cancers, including hepatocellular carcinoma (HCC), the third leading cause of cancer-related deaths worldwide. Obesity-associated hepatic tumorigenesis develops from nonalcoholic fatty liver disease (NAFLD), progressing to nonalcoholic steatohepatitis (NASH), cirrhosis and ultimately to HCC. The rising incidence of obesity is resulting in an increased prevalence of NAFLD and NASH, and subsequently HCC. Obesity represents an increasingly important underlying etiology of HCC, in particular as the other leading causes of HCC such as hepatitis infection, are declining due to effective treatments and vaccines. In this review, we provide a comprehensive overview of the molecular mechanisms and cellular signaling pathways involved in the pathogenesis of obesity-associated HCC. We summarize the preclinical experimental animal models available to study the features of NAFLD/NASH/HCC, and the non-invasive methods to diagnose NAFLD, NASH and early-stage HCC. Finally, since HCC is an aggressive tumor with a 5-year survival of less than 20%, we will also discuss novel therapeutic targets for obesity-associated HCC and ongoing clinical trials.
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- 2023
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21. Upregulation of cadherin‐11 contributes to cholestatic liver fibrosis
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Bo Wu, Xinbei Tian, Weipeng Wang, Jing Zhu, Ying Lu, Jun Du, and Yongtao Xiao
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Cadherin‐11 ,Biliary atresia ,Cholestatic liver fibrosis ,TGF‐β/Smad ,Pediatrics ,RJ1-570 - Abstract
ABSTRACT Importance Cadherin‐11 (CDH11), a cell‐to‐cell adhesion molecule, is implicated in the fibrotic process of several organs. Biliary atresia (BA) is a common cholestatic liver disease featuring cholestasis and progressive liver fibrosis in children. Cholestatic liver fibrosis may progress to liver cirrhosis and lacks effective therapeutic strategies. Currently, the role of CDH11 in cholestatic liver fibrosis remains unclear. Objective This study aimed to explore the functions of CDH11 in cholestatic liver fibrosis. Methods The expression of CDH11 in BA livers was evaluated by database analysis and immunostaining. Seven BA liver samples were used for immunostaining. The wild type (Wt) and CDH11 knockout (CDH11–/–) mice were subjected to bile duct ligation (BDL) to induce cholestatic liver fibrosis. The serum biochemical analysis, liver histology, and western blotting were used to assess the extent of liver injury and fibrosis as well as activation of transforming growth factor‐β (TGF‐β)/Smad pathway. The effect of CDH11 on the activation of hepatic stellate cell line LX‐2 cells was investigated. Results Analysis of public RNA‐seq datasets showed that CDH11 expression levels were significantly increased in livers of BA, and CDH11 was correlated with liver fibrosis in BA. BDL‐induced liver injury and liver fibrosis were attenuated in CDH11–/– mice compared to Wt mice. The protein expression levels of phosphorylated Smad2/3 were decreased in livers of CDH11–/– BDL mice compared to Wt BDL mice. CDH11 knockdown inhibited the activation of LX‐2 cells. Interpretation CDH11 plays an important role in cholestatic liver fibrosis and may represent a potential therapeutic target for cholestatic liver disease, such as BA.
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- 2022
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22. A novel SRSF3 inhibitor, SFI003, exerts anticancer activity against colorectal cancer by modulating the SRSF3/DHCR24/ROS axis
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Yawen Zhang, Mengmeng Wang, Fanyi Meng, Man Yang, Yinshuang Chen, Xuqin Guo, Weiwei Wang, Yifan Zhu, Yundi Guo, Chunlai Feng, Shen Tian, Hongjian Zhang, Huanqiu Li, Jing Sun, and Weipeng Wang
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract As the modulation of serine/arginine-rich splicing factor 3 (SRSF3) may be therapeutically beneficial to colorectal cancer (CRC) treatment, the identification of novel SRSF3 inhibitors is highly anticipated. However, pharmaceutical agents targeting SRSF3 have not yet been discovered. Here, we propose a functional SRSF3 inhibitor for CRC therapy and elucidate its antitumor mechanisms. We found high expression of SRSF3 in 70.6% CRC tissues. Silencing SRSF3 markedly inhibits the proliferation and migration of CRC cells through suppression of its target gene 24-dehydrocholesterol reductase (DHCR24). This is evidenced by the links between SRSF3 and DHCR24 in CRC tissues. The novel SRSF3 inhibitor SFI003 exhibits potent antitumor efficacy in vitro and in vivo, which drives apoptosis of CRC cells via the SRSF3/DHCR24/reactive oxygen species (ROS) axis. Moreover, SFI003 is druggable with suitable pharmacokinetic properties, bioavailability, and tumor distribution. Thus, SRSF3 is a novel potential therapeutic target for CRC. Its inhibitor SFI003 may be developed as an anticancer therapeutic.
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- 2022
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23. Recognition of dipole-induced electric field in 2D materials for surface-enhanced Raman scattering
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Yuxue Yang, Shen Ao, Jiaqi Wang, Wangyang Fu, Xiangxuan Liu, and Weipeng Wang
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two-dimensional (2D) materials ,chemical enhancement ,surface enhancement Raman scattering ,dipole interaction ,vibration selectivity ,Chemistry ,QD1-999 - Abstract
The application of two-dimensional (2D) materials, including metallic graphene, semiconducting transition metal dichalcogenides, and insulating hexagonal boron nitride (h-BN) for surface-enhancement Raman spectroscopy has attracted extensive research interest. This article provides a critical overview of the recent developments in surface-enhanced Raman spectroscopy using 2D materials. By re-examining the relationship between the lattice structure and Raman enhancement characteristics, including vibration selectivity and thickness dependence, we highlight the important role of dipoles in the chemical enhancement of 2D materials.
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- 2023
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24. Group-Based Deep Transfer Learning with Mixed Gate Control for Cross- Domain Recommendation.
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Mingze Sun, Daiyue Xue, Weipeng Wang, Qifu Hu, and Jianping Yu
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- 2021
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25. Clinical characteristics and prognosis of 196 Chinese patients with colon cancer
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Lunjin Yao, Huihui Zhang, Weipeng Wang, Xiaoxia An, Zhiqiang Cheng, Xiang Zhang, Kexin Wang, and Binbin Zhang
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colon cancer ,prognostic factors ,cox proportional hazard regression ,tumor node metastasis (TNM) staging ,tumor makers ,Surgery ,RD1-811 - Abstract
AimsTo analyze the clinical characteristics and prognostic factors of Chinese patients with colon cancer.MethodsA retrospective analysis of the records of patients with colon cancer underwent surgery between 2014 and 2017 was performed. Univariate analysis in combination with Cox proportional hazard regression model was used to analyze the survival data, so as to reveal the prognostic factors of colon cancer. Data record was based on a standard data form. SPSS version 26.0 was used for data analysis (SPSS, Chicago, IL, United States).ResultsThe 3-year survival rate and the 5-year survival rate was 79.3% and 68.2%, respectively. Univariate analysis showed that radical surgery, laparoscopic surgery, ascites, swollen lymph nodes at the root of the mesentery, liver metastases, nerve invasion, vascular invasion, tumor node metastasis (TNM) staging, positive level of carbohydrate antigen (CA) 19-9, CA125, CA72-4 and combined detection were positive factors in the prognosis of colon cancer (P
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- 2023
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26. A Deep Reinforcement Learning Framework for Vehicle Detection and Pose Estimation in 3D Point Clouds.
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Weipeng Wang, Huan Luo 0001, Quan Zheng, Cheng Wang 0003, and Wenzhong Guo
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- 2020
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27. The Functional Characterization of DzCYP72A12-4 Related to Diosgenin Biosynthesis and Drought Adaptability in Dioscorea zingiberensis
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Weipeng Wang, Lixiu Hou, Song Li, and Jiaru Li
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Dioscorea zingiberensis ,diosgenin ,Sterol C26-hydroxylase ,CYP72A ,drought stress ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Dioscorea zingiberensis is a perennial herb famous for the production of diosgenin, which is a valuable initial material for the industrial synthesis of steroid drugs. Sterol C26-hydroxylases, such as TfCYP72A616 and PpCYP72A613, play an important role in the diosgenin biosynthesis pathway. In the present study, a novel gene, DzCYP72A12-4, was identified as C26-hydroxylase and was found to be involved in diosgenin biosynthesis, for the first time in D. zingiberensis, using comprehensive methods. Then, the diosgenin heterogenous biosynthesis pathway starting from cholesterol was created in stable transgenic tobacco (Nicotiana tabacum L.) harboring DzCYP90B71(QPZ88854), DzCYP90G6(QPZ88855) and DzCYP72A12-4. Meanwhile, diosgenin was detected in the transgenic tobacco using an ultra-performance liquid chromatography system (Vanquish UPLC 689, Thermo Fisher Scientific, Bremen, Germany) tandem MS (Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer, Thermo Fisher Scientific, Bremen, Germany). Further RT-qPCR analysis showed that DzCYP72A12-4 was highly expressed in both rhizomes and leaves and was upregulated under 15% polyethylene glycol (PEG) treatment, indicating that DzCYP72A12-4 may be related to drought resistance. In addition, the germination rate of the diosgenin-producing tobacco seeds was higher than that of the negative controls under 15% PEG pressure. In addition, the concentration of malonaldehyde (MDA) was lower in the diosgenin-producing tobacco seedlings than those of the control, indicating higher drought adaptability. The results of this study provide valuable information for further research on diosgenin biosynthesis in D. zingiberensis and its functions related to drought adaptability.
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- 2023
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28. Conditional depletion of macrophages ameliorates cholestatic liver injury and fibrosis via lncRNA-H19
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Xinbei Tian, Ying Wang, Ying Lu, Weipeng Wang, Jun Du, Shanshan Chen, Huiping Zhou, Wei Cai, and Yongtao Xiao
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Cytology ,QH573-671 - Abstract
Abstract Although macrophages are recognized as important players in the pathogenesis of chronic liver diseases, their roles in cholestatic liver fibrosis remain incompletely understood. We previously reported that long noncoding RNA-H19 (lncRNA-H19) contributes to cholangiocyte proliferation and cholestatic liver fibrosis of biliary atresia (BA). We here show that monocyte/macrophage CD11B mRNA levels are increased significantly in livers of BA patients and positively correlated with the progression of liver inflammation and fibrosis. The macrophages increasingly infiltrate and accumulate in the fibrotic niche and peribiliary areas in livers of BA patients. Selective depletion of macrophages using the transgenic CD11b-diphtheria toxin receptor (CD11b-DTR) mice halts bile duct ligation (BDL)-induced progression of liver damage and fibrosis. Meanwhile, macrophage depletion significantly reduces the BDL-induced hepatic lncRNA-H19. Overexpression of H19 in livers using adeno-associated virus serotype 9 (AAV9) counteracts the effects of macrophage depletion on liver fibrosis and cholangiocyte proliferation. Additionally, both H19 knockout (H19−/−) and conditional deletion of H19 in macrophage (H19ΔCD11B) significantly depress the macrophage polarization and recruitment. lncRNA-H19 overexpressed in THP-1 macrophages enhance expression of Rho-GTPase CDC42 and RhoA. In conclusions, selectively depletion of macrophages suppresses cholestatic liver injuries and fibrosis via the lncRNA-H19 and represents a potential therapeutic strategy for rapid liver fibrosis in BA patients.
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- 2021
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29. Three-Dimensional Object Co-Localization From Mobile LiDAR Point Clouds.
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Wenzhong Guo, Jiawei Chen, Weipeng Wang, Huan Luo 0001, and Shiping Wang
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- 2021
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30. Development and Evaluation of Novel Metformin Derivative Metformin Threonate for Brain Ischemia Treatment
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Gufang Zhang, Shuangshuang Chen, Jia Jia, Chun Liu, Weipeng Wang, Hongjian Zhang, and Xuechu Zhen
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ischemic stroke ,metformin threonate ,microglia ,neuroinflammation ,functional recovery ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Epidemiologic data reveal that diabetes patients taking metformin exhibit lower incidence of stroke and better functional outcomes during post-stroke neurologic recovery. We previously demonstrated that chronic post-ischemic administration of metformin improved functional recovery in experimental cerebral ischemia. However, few beneficial effects of metformin on the acute phase of cerebral ischemia were reported either in experimental animals or in stroke patients, which limits the application of metformin in stroke. We hypothesized that slow cellular uptake of metformin hydrochloride may contribute to the lack of efficacy in acute stroke. We recently developed and patented a novel metformin derivative, metformin threonate (SHY-01). Pharmacokinetic profile in vivo and in cultured cells revealed that metformin is more rapidly uptaken and accumulated from SHY-01 than metformin hydrochloride. Accordingly, SHY-01 treatment exhibited more potent and rapid activation of AMP-activated protein kinase (AMPK). Furthermore, SHY-01 elicited a stronger inhibition of microglia activation and more potent neuroprotection when compared to metformin hydrochloride. SHY-01 administration also had superior beneficial effects on neurologic functional recovery in experimental stroke and offered strong protection against acute cerebral ischemia with reduced infarct volume and mortality, as well as the improved sensorimotor and cognitive functions in rats. Collectively, these results indicated that SHY-01 had an improved pharmacokinetic and pharmacological profile and produced more potent protective effects on acute stroke and long-term neurological damage. We propose that SHY-01 is a very promising therapeutic candidate for cerebral ischemic stroke.
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- 2022
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31. Mediastinal Shift Angle in Fetal MRI Is Associated With Prognosis, Severity, and Cardiac Underdevelopment in Left Congenital Diaphragmatic Hernia
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Xueyao Wang, Qi Shi, Weihua Pan, Weipeng Wang, Wenjie Wu, Ming Liu, Wei Xie, Xinyun Wang, and Jun Wang
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congenital diaphragmatic hernia ,prenatal diagnosis ,fetal magnetic resonance imaging ,mediastinal shift angle ,cardiac underdevelopment ,fetal predictors ,Pediatrics ,RJ1-570 - Abstract
Objective:Fetal MRI has played an essential role in the evaluation and management of congenital diaphragmatic hernia (CDH). We aimed to investigate whether the mediastinal shift angle (MSA) value was associated with the prognosis and the severity of left CDH and explore the relationship between the MSA value and fetal and neonatal cardiac structures and functions.MethodsFrom January 2012 to December 2020, the fetal MSA values of left CDH in our institution were retrospectively measured. Other prenatal parameters and clinical outcomes of them are collected. We also measured the fetal and postnatal echocardiography parameters to analyze linear correlation with MSA values.ResultsA total of 94 patients with left CDH were included. MSA was significantly higher in the deceased group than in the survived group [((38.3 ± 4.7)° vs. 32.3 ± 5.3)°, p < 0.001]. The MSA value of the high-risk defect group [CDH Study Group (CDHSG) C/D type] was significantly higher than that of the low-risk defect group [CDHSG A/B type; (36.0 ± 4.9)° vs. (30.1 ± 4.8)°, p < 0.001]. The AUC for severity was 0.766 (95% CI, 0.661–0.851, p < 0.0001) and the best cut-off value for MSA was 30.7°. Higher MSA correlates with decreased fetal Z-score of left ventricle (LV) width, the diameter of the mitral valve (MV), peak velocity of MV and tricuspid valve (TV), and neonatal LV end-diastolic diameter (LVEDD) and velocity of tricuspid regurgitation (TR; p < 0.05).ConclusionA high MSA value can effectively predict high-risk defects and high mortality of left CDH. The higher the MSA value, the worse the neonatal conditions, the respiratory and cardiovascular prognosis. The MSA values could reflect the level of left heart underdevelopment, including decreased dimensions and diastolic dysfunction of the left ventricle.
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- 2022
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32. Study on response of metal wire in thermoacoustic imaging
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Zheng Liang, Weipeng Wang, Shuaiqi Qiao, and Lin Huang
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Thermoacoustic imaging ,simulation ,metal wire ,antenna ,Technology ,Optics. Light ,QC350-467 - Abstract
Thermoacoustic imaging (TAI) is an emerging high-resolution and high-contrast imaging technology. In recent years, metal wires have been used in TAI experiments to quantitatively evaluate the spatial resolution of different systems. However, there is still a lack of analysis of the response characteristics and principles of metal wires in TAI. Through theoretical and simulation analyses, this paper proposes that the response of metal (copper) wires during TAI is equivalent to the response of antennas. More critically, the response of the copper wire is equivalent to the response of a half-wave dipole antenna. When its length is close to half the wavelength of the incident electromagnetic wave, it obtains the best response. In simulation, when the microwave excitation frequencies are 1.3[Formula: see text]GHz, 3.0[Formula: see text]GHz, and 5.3[Formula: see text]GHz, and the lengths of copper wires are separately set to 11[Formula: see text]cm, 5[Formula: see text]cm, and 2.5[Formula: see text]cm, the maximum SAR distribution and energy coupling efficiency are obtained. This result is connected with the best response of half-wave dipole antennas with lengths of 11[Formula: see text]cm, 4.77[Formula: see text]cm, and 2.7[Formula: see text]cm under the theoretical design, respectively. Regarding the further application, TAI can be used to conduct guided minimally invasive surgery on surgical instrument imaging. Thus, this paper indicated that results can also guide the design of metal surgical instruments utilized in different microwave frequencies.
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- 2022
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33. Genome-Wide Identification and Evolutionary Analysis of Gossypium YTH Domain-Containing RNA-Binding Protein Family and the Role of GhYTH8 in Response to Drought Stress
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Wei Hao, Weipeng Wang, Xiangfen Xiao, Jialiang Sun, Bingjie Wu, Yuping Zhao, Shuaishuai Pei, Wenjia Fan, Dongbei Xu, and Tengfei Qin
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Gossypium ,drought stress ,YTH domain-containing RNA-binding protein ,gene family ,Botany ,QK1-989 - Abstract
YTH domain-containing proteins are one kind of RNA-binding protein involved in post-transcriptional regulation and play multiple roles in regulating the growth, development, and abiotic stress responses of plants. However, the YTH domain-containing RNA-binding protein family has not been previously studied in cotton. In this study, a total of 10, 11, 22, and 21 YTH genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium barbadense, and Gossypium hirsutum, respectively. These Gossypium YTH genes were categorized into three subgroups by phylogenetic analysis. The chromosomal distribution, synteny analysis, structures of Gossypium YTH genes, and the motifs of YTH proteins were analyzed. Furthermore, the cis-element of GhYTH genes promoter, miRNA targets of GhYTH genes, and subcellular localization of GhYTH8 and GhYTH16 were characterized. Expression patterns of GhYTH genes in different tissues, organs, and in response to different stresses were also analyzed. Moreover, functional verifications revealed that silencing GhYTH8 attenuated the drought tolerance in the upland cotton TM-1 line. These findings provide useful clues for the functional and evolutionary analysis of YTH genes in cotton.
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- 2023
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34. Recent research on material-based methods for isolation of extracellular vesicles.
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Mengxi Chen, Jiaxi Li, Yujie Lin, Xiaowei Li, Yuanyuan Yu, Shenyue Zhou, Fang Xu, Qi Zhang, Haiyang Zhang, and Weipeng Wang
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- 2024
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35. Wearable Wristworn Gesture Recognition Using Echo State Network.
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Weipeng Wang, Xiangpeng Liang, Maher Assaad, and Hadi Heidari
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- 2019
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36. Study on Medical Image Report Generation Based on Improved Encoding-Decoding Method.
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Li Sun, Weipeng Wang, Jiyun Li, and Jingsheng Lin
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- 2019
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37. Joint optimization algorithm of user association and power control based on spatial traffic distribution.
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Weipeng Wang, Jihong Zhao 0001, Hua Qu, Yanpeng Zhang, and Gongye Ren
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- 2022
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38. SRSF3 Promotes Angiogenesis in Colorectal Cancer by Splicing SRF
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Yinshuang Chen, Man Yang, Fanyi Meng, Yawen Zhang, Mengmeng Wang, Xuqin Guo, Jie Yang, Hongjian Zhang, Haiyang Zhang, Jing Sun, and Weipeng Wang
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angiogenesis ,colorectal cancer ,splicing ,SRF ,SRSF3 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
SRSF3, an important member of the serine/arginine-rich protein (SRp) family, is highly expressed in various tumors and plays an important role in tumor cell proliferation, migration and invasion. However, it is still unclear whether SRSF3 is involved in tumor angiogenesis. In this study, we first revealed that SRSF3 regulated the expression of numerous genes related to angiogenesis, including proangiogenic SRF. Then, we confirmed that SRSF3 was highly expressed in colorectal cancer (CRC) and was positively correlated with SRF. Mechanistic studies revealed that SRSF3 directly bound to the “CAUC” motif in exon 6 of SRF and induced the exclusion of introns. Knockdown of SRSF3 significantly reduced the secretion of VEGF from CRC cells. Conditioned medium from SRSF3-knockdown CRC cells significantly inhibited the migration, invasion and tube formation of human umbilical vein endothelial cells (HUVECs). In addition, SRF silencing inhibited angiogenesis, while SRF overexpression reversed the antiangiogenic effects of SRSF3 knockdown on tube formation. These findings indicate that SRSF3 is involved in the splicing of SRF and thereby regulates the angiogenesis of CRC, which offers novel insight into antiangiogenic therapy in CRC.
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- 2022
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39. Can early surgery improve the outcome of patients with meconium peritonitis? A single-center experience over 16 years
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Yi Jiang, Weihua Pan, Wenjie Wu, Weipeng Wang, Suna Sun, and Jun Wang
- Subjects
Meconium peritonitis ,Early operation ,Survival rate ,Premature ,Risk factor ,Pediatrics ,RJ1-570 - Abstract
Abstract Background In the last century, meconium peritonitis(MP)was once a highly fatal gastrointestinal. disease With the development of fetal radiological technology, abnormal signs, such as pseudocysts, can. be detected during the fetal period so that more patients can be diagnosed prenatally and receive surgery. in the early stage of life. The survival rate of MP has increased up to 80% in recent years. According to. a review of the treatment and outcomes of patients diagnosed with MP, we evaluated the influence of. early operation on survival rate and discussed the risk factors of prognosis. Methods We collected 79 cases of patients diagnosed with MP who were treated in our department. from October 2001 to December 2017. They were divided into 2 groups. Patients in group A were born. in our hospital. Patients in group B were born in a local hospital with suspicion of MP and then transferred. to our department. Results The birth weight (BW) and gestational age (GA) of patients were higher in group A than in. group B. There was no significant difference in the proportion of premature and low birth weight (LBW). patients between the two groups (p = 0.422, p = 0.970). Their age at the time of surgery was younger in. group A than in group B (1.4 ± 2.0 vs. 6.9 ± 14.9, p
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- 2019
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40. Joint Optimization Algorithm for Small Base Station States Control and User Association in Wireless Caching Networks
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Weipeng Wang, Jihong Zhao, Hua Qu, and Huijun Dai
- Subjects
cache network ,energy efficient ,service delay ,SBS state control ,user association ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The energy consumption management of small base stations (SBSs) in wireless caching networks with dense deployment of SBSs is an urgent problem to be solved. This paper jointly optimizes the SBS state control and user association problems in caching networks to reduce network energy consumption while taking into account the average service latency of the network to ensure user experience. First, a new definition of three-state SBSs in caching networks is proposed based on their ability to keep content cache updated. Then, a relaxed threshold setting method is designed and the SBS traffic prediction is used to obtain the initial state information of SBSs in the next period. In order to eliminate the impact on the accessing users when the switched-off base station (BS) wakes up, a SBS state asynchronous switching mechanism is proposed to ensure that the users who switch to the waking SBS can carry out communication services normally, and a user association strategy is constructed with the SBS load as the optimization target. Finally, a joint optimization model of user association and SBS state control (SSC-UA) is constructed to admit and correct the initial state of SBSs to maximize the system gain and obtain the final state strategy for each SBS in the next period. The simulation results show that the proposed SSC-UA algorithm can effectively improve the energy efficiency and reduce the network service delay at the same time.
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- 2022
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41. Research Progress of PPR Proteins in RNA Editing, Stress Response, Plant Growth and Development
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Tengfei Qin, Pei Zhao, Jialiang Sun, Yuping Zhao, Yaxin Zhang, Qiuyue Yang, Weipeng Wang, Zhuanqing Chen, Tengfei Mai, Yingying Zou, Guoxiang Liu, and Wei Hao
- Subjects
pentatricopeptide repeat ,RNA editing ,biogenesis ,development ,mechanism ,Genetics ,QH426-470 - Abstract
RNA editing is a posttranscriptional phenomenon that includes gene processing and modification at specific nucleotide sites. RNA editing mainly occurs in the genomes of mitochondria and chloroplasts in higher plants. In recent years, pentatricopeptide repeat (PPR) proteins, which may act as trans-acting factors of RNA editing have been identified, and the study of PPR proteins has become a research focus in molecular biology. The molecular functions of these proteins and their physiological roles throughout plant growth and development are widely studied. In this minireview, we summarize the current knowledge of the PPR family, hoping to provide some theoretical reference for future research and applications.
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- 2021
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42. RNA Editing and Its Roles in Plant Organelles
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Wei Hao, Guoxiang Liu, Weipeng Wang, Wei Shen, Yuping Zhao, Jialiang Sun, Qiuyue Yang, Yaxin Zhang, Wenjia Fan, Shuaishuai Pei, Zhuanqing Chen, Dongbei Xu, and Tengfei Qin
- Subjects
RNA editing ,factors ,mechanism ,plant organelles ,deep sequencing ,Genetics ,QH426-470 - Abstract
RNA editing, a vital supplement to the central dogma, yields genetic information on RNA products that are different from their DNA templates. The conversion of C-to-U in mitochondria and plastids is the main kind of RNA editing in plants. Various factors have been demonstrated to be involved in RNA editing. In this minireview, we summarized the factors and mechanisms involved in RNA editing in plant organelles. Recently, the rapid development of deep sequencing has revealed many RNA editing events in plant organelles, and we further reviewed these events identified through deep sequencing data. Numerous studies have shown that RNA editing plays essential roles in diverse processes, such as the biogenesis of chloroplasts and mitochondria, seed development, and stress and hormone responses. Finally, we discussed the functions of RNA editing in plant organelles.
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- 2021
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43. SemRec: a personalized semantic recommendation method based on weighted heterogeneous information networks.
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Chuan Shi, Zhiqiang Zhang 0012, Yugang Ji, Weipeng Wang, Philip S. Yu, and Zhiping Shi 0002
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- 2019
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44. Joint caching policies for optimizing energy costs/offloading probability for D2D and millimeter-wave small cell underlaying cache-enabled networks.
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Jihong Zhao 0001, Weipeng Wang, Hua Qu, Shuyuan Zhao, and Gongye Ren
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- 2020
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45. Genome-Wide Identification of Cotton (Gossypium spp.) Trehalose-6-Phosphate Phosphatase (TPP) Gene Family Members and the Role of GhTPP22 in the Response to Drought Stress
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Weipeng Wang, Hua Cui, Xiangfen Xiao, Bingjie Wu, Jialiang Sun, Yaxin Zhang, Qiuyue Yang, Yuping Zhao, Guoxiang Liu, and Tengfei Qin
- Subjects
cotton ,trehalose-6-phosphate phosphatase (TPP) ,gene expression ,drought stress ,trehalose 6-phosphate (T6P) ,Botany ,QK1-989 - Abstract
Trehalose-6-phosphate phosphatase (TPP) is a key enzyme involved in trehalose synthesis in higher plants. Previous studies have shown that TPP family genes increase yields without affecting plant growth under drought conditions, but their functions in cotton have not been reported. In this study, 17, 12, 26 and 24 TPP family genes were identified in Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively. The 79 TPP family genes were divided into three subgroups by phylogenetic analysis. Virus-induced gene silencing (VIGS) of GhTPP22 produced TRV::GhTPP22 plants that were more sensitive to drought stress than the control plants, and the relative expression of GhTPP22 was decreased, as shown by qRT–PCR. Moreover, we analysed the gene structure, targeted small RNAs, and gene expression patterns of TPP family members and the physicochemical properties of their encoded proteins. Overall, members of the TPP gene family in cotton were systematically identified, and the function of GhTPP22 under drought stress conditions was preliminarily verified. These findings provide new information for improving drought resistance for cotton breeding in the future.
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- 2022
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46. Genome-Wide Identification of Cotton (Gossypium spp.) Glycerol-3-Phosphate Dehydrogenase (GPDH) Family Members and the Role of GhGPDH5 in Response to Drought Stress
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Jialiang Sun, Hua Cui, Bingjie Wu, Weipeng Wang, Qiuyue Yang, Yaxin Zhang, Song Yang, Yuping Zhao, Dongbei Xu, Guoxiang Liu, and Tengfei Qin
- Subjects
drought stress ,replication events ,transcriptional expression profiles ,protein-protein interaction network ,stomatal aperture ,Botany ,QK1-989 - Abstract
Glycerol-3-phosphate dehydrogenase (GPDH) is a key enzyme in plant glycerol synthesis and metabolism, and plays an important role in plant resistance to abiotic stress. Here, we identified 6, 7, 14 and 14 GPDH genes derived from Gossypium arboreum, Gossypium raimondii, Gossypium barbadense and Gossypium hirsutum, respectively. Phylogenetic analysis assigned these genes into three classes, and most of the genes within the family were expanded by whole-genome duplication (WGD) and segmental duplications. Moreover, determination of the nonsynonymous substitution rate/synonymous substitution rate (Ka/Ks) ratio showed that the GPDH had an evolutionary preference for purifying selection. Transcriptome data revealed that GPDH genes were more active in the early stages of fiber development. Additionally, numerous stress-related cis-elements were identified in the potential promoter region. Then, a protein–protein-interaction (PPI) network of GPDH5 in G. hirsutum was constructed. In addition, we predicted 30 underlying miRNAs in G. hirsutum. Functional validation results indicated that silencing GhGPDH5 diminished drought tolerance in the upland cotton TM-1 line. In summary, this study provides a fundamental understanding of the GPDH gene family in cotton, GhGPDH5 exerts a positive effect during drought stress and is potentially involved in stomatal closure movements.
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- 2022
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47. Low Frequency Terahertz Induced Thermoacoustic Signal Characteristics and Its Application in Solid-State Terahertz Transmitter Power Detection
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Weipeng Wang, Lin Huang, Hongji Zhou, Sen Gong, Hongxin Zeng, Jun Zhou, Ziqiang Yang, and Yaxin Zhang
- Subjects
Radiation ,Electrical and Electronic Engineering - Published
- 2022
48. Recent advances in Raman and surface enhanced Raman spectroelectrochemistry
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Yue, Kang, primary, Weipeng, Wang, additional, Tian, Ma, additional, Ting, Zou, additional, Junxian, Chen, additional, and Zhengjun, Zhang, additional
- Published
- 2023
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49. Hierarchical structure of amorphous bimetallic hydroxide modified Co-metal organic framework catalyst efficient and robust for oxygen evolution reaction
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Yuanyuan Wu, Weipeng Wang, Wei Jiang, Hongji Li, Yunchao Ma, Chunbo Liu, and Xiaotian Yang
- Subjects
Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,Condensed Matter Physics - Published
- 2022
50. Biomimetic noncationic lipid nanoparticles for mRNA delivery.
- Author
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Changrong Wang, Caiyan Zhao, Weipeng Wang, Xiaoqing Liu, and Hongzhang Deng
- Subjects
GENE transfection ,MESSENGER RNA ,CATIONIC lipids ,GENE expression ,HYDROGEN bonding interactions ,POLYETHYLENEIMINE - Abstract
Although the tremendous progress has been made for mRNA delivery based on classical cationic carriers, the excess cationic charge density of lipids was necessary to compress mRNA through electrostatic interaction, and with it comes inevitably adverse events including the highly inflammatory and cytotoxic effects. How to develop the disruptive technologies to overcome cationic nature of lipids remains a major challenge for safe and efficient mRNA delivery. Here, we prepared noncationic thiourea lipids nanoparticles (NC-TNP) to compress mRNA by strong hydrogen bonds interaction between thiourea groups of NC-TNP and the phosphate groups of mRNA, abandoning the hidebound and traditional electrostatic force to construct mRNA-cationic lipids formulation. NC-TNP was a delivery system for mRNA with simple, convenient, and repeatable preparation technology and showed negligible inflammatory and cytotoxicity side effects. Furthermore, we found that NC-TNP could escape the recycling pathway to inhibit the egress of internalized nanoparticles from the intracellular compartment to the extracellular milieu which was a common fact in mRNA-LNP (lipid nanoparticles) formulation. Therefore, NC-TNP- encapsulated mRNA showed higher gene transfection efficiency in vitro and in vivo than mRNA-LNP formulation. Unexpectedly, NC-TNP showed spleen targeting delivery ability with higher accumulation ratio (spleen/liver), compared with traditional LNP. Spleen-targeting NC-TNP with mRNA exhibited high mRNA-encoded antigen expression in spleen and elicited robust immune responses. Overall, our work establishes a proof of concept for the construction of a noncationic system for mRNA delivery with good inflammatory safety profiles, high gene transfection efficiency, and spleen-targeting delivery to induce permanent and robust humoral and cell-mediated immunity for disease treatments. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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