101 results on '"Vandenbussche C"'
Search Results
2. Perception de la notion de « violences obstétricales » par les professionnels et futurs professionnels de la santé : étude transversale par questionnaire
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Vandenbussche, C., primary, Costa, E., additional, Huberland, V., additional, and Donner, C., additional
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- 2023
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3. Perception de la notion de « violences obstétricales » par les professionnels et futurs professionnels de la santé :étude transversale par questionnaire
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Vandenbussche, C., Costa, Elena, Huberland, Vincent, donner, catherine, Vandenbussche, C., Costa, Elena, Huberland, Vincent, and donner, catherine
- Abstract
Le point de vue des soignants a été encore peu investigué dans le cadre des « violences obstétricales ». Nous avons interrogé des gynécologues-obstétricien.ne.s, assistant.e.s en gynécologie-obstétrique et étudiant.e.s en médecine de dernière année à l’aide d’un questionnaire en ligne. Deux cent cinquante-huit questionnaires ont été complétés et montrent que la problématique est une réalité, les médecins en sont conscients, et leur définition du phénomène rejoint celle des patientes. Une divergence existe cependant entre les points de vue théorique et pratique., info:eu-repo/semantics/published
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- 2023
4. Chapter 17: Practical experiences of IoT applications for pig, broiler and cattle beef production: IoF2020 meat trial
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Maselyne, J., Vandenbussche, C., Fernández, I., García, E., Kassahun, A., Bugueiro, A., Gómez-Maqueda, I., and Gelada, J.
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Life Science ,Toegepaste Informatiekunde ,WASS ,Information Technology - Abstract
Internet of Things (IoT) technology offers a significant potential for livestock farming, as animals and their living environments can be monitored and relevant insights can be extracted from the data gathered. As part of the IoF2020 project (Internet of Food & Farm 2020, 2017-2021, EU H2020 programme) IoT devices were tested and demonstrated in six use cases for livestock farming for meat production. The results and lessons learned give insights in how IoT devices can technically and practically be used in livestock farming and how companies can create businesses from the technologies. The use cases are part of the meat trial of the IoF2020 project and each developed IoTbased products to solve one or more specific challenges that the livestock sector faces. The products were developed in close collaboration with the end-users, who are mainly livestock farmers and meat processors, who helped to formulate requirements, provided their facilities as a testbed and gave feedback to improve the products. The use cases cover three livestock species: pigs, broilers and beef cattle. Diverse IoT solutions and products were developed, including sensors for health and welfare monitoring, environmental monitoring, location tracking and feed silo monitoring. In addition, business intelligence dashboards, transparency/traceability systems and auditing services were also developed. The use cases had installations in 64 sites (farms, slaughterhouses) in 10 different EU countries. In this chapter, we present the objectives, results, challenges and lessons learned from the use cases. We also discuss the stakeholders involved and the business models that have been developed. Feedback from end-users proved to be crucial for steering the developments in the right direction.
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- 2022
5. The fine needle aspiration of translocation sarcomas
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VandenBussche, C. J., Adams, C. L., McDonald, O. G., Whitworth, S. A., and Ali, S. Z.
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- 2017
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6. L’amylose cardiaque en hémodialyse : une entité sous-diagnostiquée ?
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Cartery, C., primary, Ulrich, M., additional, Vandenbussche, C., additional, Guerry, M.J., additional, and Goubet, M., additional
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- 2021
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7. Dramatically reduced surface expression of NK cell receptor KIR2DS3 is attributed to multiple residues throughout the molecule
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VandenBussche, C J, Mulrooney, T J, Frazier, W R, Dakshanamurthy, S, and Hurley, C K
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- 2009
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8. Epidemiological features and prognosis of severe community-acquired pneumococcal pneumonia
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Georges, H., Leroy, O., Vandenbussche, C., Guery, B., Alfandari, S., Tronchon, L., and Beaucaire, G.
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- 1999
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9. Severe community-acquired pneumonia in ICUs: Prospective validation of a prognostic score
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Leroy, O., Georges, H., Beuscart, C., Guery, B., Coffinier, C., Vandenbussche, C., Thevenin, D., and Beaucaire, G.
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- 1996
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10. Insuffisance rénale aiguë et leucémie aiguë myéloblastique
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Bouderlique, E., primary, Vandenbussche, C., additional, Gnemmi, V., additional, Cambier, N., additional, and Ulrich, M., additional
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- 2019
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11. Reliability of quantitative cultures of protected specimen brush after freezing.
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Georges, H, Santre, C, Leroy, O, Roussel-Delvallez, M, Caillaux, M, Beuscart, C, Guery, B, Vandenbussche, C, and Beaucaire, G
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- 1996
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12. Implication de miR-21 dans les agressions rénales
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Van Der Hauwaert, C., primary, Hennino, M.F., additional, Vandenbussche, C., additional, Dewaeles, E., additional, Gnemmi, V., additional, Savary, G., additional, Pottier, N., additional, Glowacki, F., additional, and Cauffiez, C., additional
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- 2018
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13. Impact de la spécificité des ANCA sur la survie rénale : à propos d’une série rétrospective observationnelle multicentrique
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Cartery, C., primary, Puigrenier, S., additional, Bitton, L., additional, Gnemmi, V., additional, Quemeneur, T., additional, and Vandenbussche, C., additional
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- 2018
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14. Urinary cytology and the Paris system for reporting urinary cytology: Implications for urological management
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Gupta, M., primary, VandenBussche, C. J., additional, and Bivalacqua, T. J., additional
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- 2018
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15. Glomérulonéphrite associée aux anticorps anti-cytoplasme des polynucléaires neutrophiles (ANCA) : valeur pronostique de la fibrose interstitielle
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Bitton, L., primary, Vandenbussche, C., additional, Cordonnier, C., additional, Verine, J., additional, Bataille, P., additional, Azar, R., additional, Namara, E. Mac, additional, Hatron, P.Y., additional, Glowacki, F., additional, Copin, M.C., additional, Quéméneur, T., additional, and Gnemmi, V., additional
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- 2017
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16. Étude des facteurs pronostiques cliniques et histologiques à partir d’une série de 113 patients atteints d’une vascularite rénale à ANCA
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Bitton, L., primary, Vandenbussche, C., additional, Cordonnier, C., additional, Verine, J., additional, Bataille, P., additional, Azar, R., additional, Namara, E. Mac, additional, Hatron, P.Y., additional, Glowacki, F., additional, Copin, M.C., additional, Quemeneur, T., additional, and Gnemmi, V., additional
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- 2017
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17. Valeur pronostique de l’hématurie lors de la rémission au décours d’une première poussée de glomérulonéphrite extracapillaire pauci-immune (GNEC)
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Vandenbussche, C., primary, Bitton, L., additional, Bataille, P., additional, Glowacki, F., additional, Azar, R., additional, Hatron, P.Y., additional, Namara, E. Mac, additional, Gheerbrant, J.D., additional, Cardon, G., additional, Hoffmann, M., additional, Gnemmi, V., additional, and Quemeneur, T., additional
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- 2017
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18. Examens bactériologiques : exploitons leurs résultats !
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Siauve, J., primary, Boursier, A., additional, Noulard, M.N., additional, Vandenbussche, C., additional, El Samad, Y., additional, Patte, I., additional, and Adjodah, C., additional
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- 2017
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19. Analytical validity of a microRNA-based assay for diagnosing indeterminate thyroid FNA smears from routinely prepared cytology slides
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Benjamin, H, Schnitzer Perlman, T, Shtabsky, A, Vandenbussche, C, Ali, S, Kolar, Z, Pagni, F, Bar, D, Meiri, E, Meiri, E., Benjamin, H, Schnitzer Perlman, T, Shtabsky, A, Vandenbussche, C, Ali, S, Kolar, Z, Pagni, F, Bar, D, Meiri, E, and Meiri, E.
- Abstract
BACKGROUND: The majority of thyroid nodules are diagnosed using fine-needle aspiration (FNA) biopsies. The authors recently described the clinical validation of a molecular microRNA-based assay, RosettaGX Reveal, which can diagnose thyroid nodules as benign or suspicious using a single stained FNA smear. This paper describes the analytical validation of the assay. METHODS: More than 800 FNA slides were tested, including slides stained with Romanowsky-type and Papanicolaou stains. The assay was examined for the following features: intranodule concordance, effect of stain type, minimal acceptable RNA amounts, performance on low numbers of thyroid cells, effect of time since sampling, and analytical sensitivity, specificity, and reproducibility. RESULTS: The assay can be run on FNA slides for which as little as 1% of the cells are thyroid epithelial cells or from which only 5 ng of RNA have been extracted. Samples composed entirely of blood failed quality control and were not classified. Stain type did not affect performance. All slides were stored at room temperature. However, the length of time between FNA sampling and processing did not affect assay performance. There was a high level of concordance between laboratories (96%), and the concordance for slides created from the same FNA pass was 93%. CONCLUSIONS: The microRNA-based assay was robust to various physical processing conditions and to differing sample characteristics. Given the assay's performance, robustness, and use of routinely prepared FNA slides, it has the potential to provide valuable aid for physicians in the diagnosis of thyroid nodules. Cancer Cytopathol 2016;124:711–21.
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- 2016
20. Glomérulonéphrites à dépôts monoclonaux non organisés, non Randall : rapport de 21 cas
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Ulrich, M., primary, Gnemmi, V., additional, Frimat, M., additional, Lionet, A., additional, Vandenbussche, C., additional, Lemoine, C., additional, Cardon, G., additional, Bataille, P., additional, Vrigneaud, L., additional, Noel, C., additional, and Lebas, C., additional
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- 2016
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21. A review of the Paris system for reporting urinary cytology
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VandenBussche, C. J., primary
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- 2016
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22. Étude anatomopathologique de la glomérulonéphrite extra-membraneuse après transplantation de mœlle osseuse
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Vandenbussche, C., primary, Lionet, A., additional, Gnemmi, V., additional, Magro, L., additional, Filali, A., additional, Ulrich, M., additional, Lemoine, C., additional, Hazzan, M., additional, and Noel, C., additional
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- 2015
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23. Nocardiose disséminée en transplantation rénale : intérêt des β-d-glucanes
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Ulrich, M., primary, Frimat, M., additional, Titcat, M., additional, Jacobsoone-Ulrich, A., additional, El Fallah, S., additional, Vandenbussche, C., additional, Lemoine, C., additional, Lionet, A., additional, Wallet, F., additional, Lebas, C., additional, Hazzan, M., additional, and Noël, C., additional
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- 2015
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24. Dramatically reduced surface expression of NK cell receptor KIR2DS3 is attributed to multiple residues throughout the molecule
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VandenBussche, C J, primary, Mulrooney, T J, additional, Frazier, W R, additional, Dakshanamurthy, S, additional, and Hurley, C K, additional
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- 2008
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25. Surmortalite liee a la pneumonie nosocomiale au cours de la ventilation des pneumonies communautaires: Etude cas-temoin
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Guilley, J, primary, Bosquet, C, additional, Vandenbussche, C, additional, Coffinier, C, additional, Leroy, O, additional, Georges, H, additional, Guery, B, additional, and Beaucaire, G., additional
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- 1997
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26. The use of problem-based learning in dealing with cultural minority groups
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Ojanlatva, A., Vandenbussche, C., Heldt, H., Horte, A., Haeggblom, T.-M., Kero, J., Kaehkoenen, J., Moettoenen, M., Saraste, A., and Turunen, T.
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- 1997
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27. Analyse des pratiques et connaissances sur les accidents d'exposition au sang
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Asseray, N., Alfandari, S., Vandenbussche, C., Guery, B., Parent, K., Georges, H., Leroy, O., Beaucaire, G., and Areclin
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- 1998
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28. CL1-01 Pneumonies communautaires sévères validation d'un score pronostique
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Mikolajczyk, D., Devos, P., Vandenbussche, C., Cabaret, P., d'Escrivan, T., Kohler, J., and Leroy, O.
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- 2004
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29. Analytical validity of a microRNA-based assay for diagnosing indeterminate thyroid FNA smears from routinely prepared cytology slides
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Zdenek Kolar, Hila Benjamin, Eti Meiri, Fabio Pagni, Alexander Shtabsky, Syed Z. Ali, Temima Schnitzer-Perlman, Dganit Bar, Christopher J. VandenBussche, Benjamin, H, Schnitzer Perlman, T, Shtabsky, A, Vandenbussche, C, Ali, S, Kolar, Z, Pagni, F, Bar, D, and Meiri, E
- Subjects
0301 basic medicine ,Thyroid nodules ,Cancer Research ,medicine.medical_specialty ,Pathology ,nodule ,smears ,Cytodiagnosis ,Concordance ,Biopsy, Fine-Needle ,fine‐needle aspiration (FNA) ,Papanicolaou stain ,Carcinoma, Papillary, Follicular ,Validation Studies as Topic ,Stain ,Specimen Handling ,thyroid ,03 medical and health sciences ,0302 clinical medicine ,Biomarkers, Tumor ,Humans ,Medicine ,Sampling (medicine) ,Thyroid Neoplasms ,Thyroid Nodule ,fine-needle aspiration (FNA) ,Thyroid Epithelial Cells ,microRNA ,business.industry ,Thyroid ,smear ,Reproducibility of Results ,indeterminate ,Original Articles ,Prognosis ,medicine.disease ,molecular test ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Molecular Diagnostic Techniques ,Oncology ,Cytopathology ,030220 oncology & carcinogenesis ,Original Article ,Radiology ,business ,nodules - Abstract
BACKGROUND The majority of thyroid nodules are diagnosed using fine‐needle aspiration (FNA) biopsies. The authors recently described the clinical validation of a molecular microRNA‐based assay, RosettaGX Reveal, which can diagnose thyroid nodules as benign or suspicious using a single stained FNA smear. This paper describes the analytical validation of the assay. METHODS More than 800 FNA slides were tested, including slides stained with Romanowsky‐type and Papanicolaou stains. The assay was examined for the following features: intranodule concordance, effect of stain type, minimal acceptable RNA amounts, performance on low numbers of thyroid cells, effect of time since sampling, and analytical sensitivity, specificity, and reproducibility. RESULTS The assay can be run on FNA slides for which as little as 1% of the cells are thyroid epithelial cells or from which only 5 ng of RNA have been extracted. Samples composed entirely of blood failed quality control and were not classified. Stain type did not affect performance. All slides were stored at room temperature. However, the length of time between FNA sampling and processing did not affect assay performance. There was a high level of concordance between laboratories (96%), and the concordance for slides created from the same FNA pass was 93%. CONCLUSIONS The microRNA‐based assay was robust to various physical processing conditions and to differing sample characteristics. Given the assay's performance, robustness, and use of routinely prepared FNA slides, it has the potential to provide valuable aid for physicians in the diagnosis of thyroid nodules. Cancer Cytopathol 2016;124:711–21. © 2016 Rosetta Genomics. Cancer Cytopathology published by Wiley Periodicals, Inc. on behalf of American Cancer Society., Analytical validation of a novel molecular assay for the diagnosis of thyroid nodules with indeterminate cytology using stained fine‐needle aspiration smears is described. The results demonstrate the assay's robustness to various physical processing conditions.
- Published
- 2016
30. The Current and Future Impact of Cytopathology on Patient Care.
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VandenBussche C
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- Humans, Patient Care trends, Pathology, Surgical trends, Pathology, Surgical methods, Cytology, Cytodiagnosis trends, Cytodiagnosis methods
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- 2024
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31. A predictive mortality score in ANCA-associated renal vasculitis.
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Fage N, Quéméneur T, Riou J, Boud'hors C, Desouche A, Vinatier E, Samoreau C, Coindre JP, Djema A, Henry N, Gnemmi V, Copin MC, Piccoli GB, Vandenbussche C, Augusto JF, and Brilland B
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- Humans, Male, Female, Prognosis, Middle Aged, Aged, Registries, Survival Rate, Follow-Up Studies, Kidney Diseases mortality, Kidney Diseases etiology, Kidney Diseases diagnosis, Risk Factors, Retrospective Studies, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis mortality, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis diagnosis, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis complications
- Abstract
Background: Several scores have been developed to predict mortality at anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) diagnosis. Their prognostic value in Caucasian patients with kidney involvement (AAV-GN) remains uncertain as none has been developed in this specific population. We aimed to propose a novel and more accurate score specific for them., Methods: This multicentric study included patients diagnosed with AAV-GN since January 2000 in four nephrology centers (recorded in the Maine-Anjou AAV-GN Registry). Existing scores and baseline characteristics were assessed at diagnosis before any therapeutic intervention. A multivariable analysis was performed to build a new predictive score for death. Its prognosis performance (area under receiving operating curve and C-index) and accuracy (Brier score) was compared with existing scores. One hundred and eighty-five patients with AAV-GN from the RENVAS registry were used as a validation cohort., Results: A total of 228 patients with AAV-GN from the Maine-Anjou registry were included to build the new score. It included the four components most associated with death: age, history of hypertension or cardiac disease, creatinine and hemoglobin levels at diagnosis. Overall, 194 patients had all the data available to determine the performance of the new score and existing scores. The new score performed better than the previous ones in the development and in the validation cohort. Among the scores tested, only Five-Factor Score and Japanese Vasculitis Activity Score had good performance in predicting death in AAV-GN., Conclusions: This original score, named DANGER (Death in ANCA Glomerulonephritis-Estimating the Risk), may be useful to predict the risk of death in AAV-GN patients. Validation in different populations is needed to clarify its role in assisting clinical decisions., (© The Author(s) 2024. Published by Oxford University Press on behalf of the ERA.)
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- 2024
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32. Pancreaticobiliary Cytology Practice in 2021: Results of a College of American Pathologists Survey.
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Li Z, Tabbara SO, Nwosu A, Souers RJ, Goyal A, Kurian EM, Lin X, VandenBussche C, and Nguyen LN
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- Humans, Biliary Tract pathology, Biliary Tract cytology, Biopsy, Fine-Needle methods, Pancreas pathology, Pancreatic Neoplasms pathology, Pancreatic Neoplasms diagnosis, Pathology, Clinical methods, Practice Patterns, Physicians' statistics & numerical data, Societies, Medical, Surveys and Questionnaires, United States, Cytodiagnosis methods, Pathologists
- Abstract
Context.—: The College of American Pathologists (CAP) surveys provide national benchmarks of pathology practice., Objective.—: To investigate pancreaticobiliary cytology practice in domestic and international laboratories in 2021., Design.—: We analyzed data from the CAP Pancreaticobiliary Cytology Practice Supplemental Questionnaire that was distributed to laboratories participating in the 2021 CAP Nongynecologic Cytopathology Education Program., Results.—: Ninety-three percent (567 of 612) of respondent laboratories routinely evaluated pancreaticobiliary cytology specimens. Biliary brushing (85%) was the most common pancreaticobiliary cytology specimen evaluated, followed by pancreatic fine-needle aspiration (79%). The most used sampling methods reported by 235 laboratories were 22-gauge needle for fine-needle aspiration (62%) and SharkCore needle for fine-needle biopsy (27%). Cell block was the most used slide preparation method (76%), followed by liquid-based cytology (59%) for pancreatic cystic lesions. Up to 95% (303 of 320) of laboratories performed rapid on-site evaluation (ROSE) on pancreatic solid lesions, while 56% (180 of 320) performed ROSE for cystic lesions. Thirty-six percent (193 of 530) of laboratories used the Papanicolaou Society of Cytopathology System for Reporting Pancreaticobiliary Cytology in 2021. Among all institution types, significant differences in specimen volume, specimen type, ROSE practice, and case sign-out were identified. Additionally, significant differences in specimen type, slide preparation, and ROSE practice were found., Conclusions.—: This is the first survey from the CAP to investigate pancreaticobiliary cytology practice. The findings reveal significant differences among institution types and between domestic and international laboratories. These data provide a baseline for future studies in a variety of practice settings., Competing Interests: The authors are or were members of the College of American Pathologists Cytopathology Committee. Nwosu and Souers are employees of the College of American Pathologists., (© 2024 College of American Pathologists.)
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- 2024
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33. Prospective, Multi-Institutional, Real-Time Next-Generation Sequencing of Pancreatic Cyst Fluid Reveals Diverse Genomic Alterations That Improve the Clinical Management of Pancreatic Cysts.
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Paniccia A, Polanco PM, Boone BA, Wald AI, McGrath K, Brand RE, Khalid A, Kubiliun N, O'Broin-Lennon AM, Park WG, Klapman J, Tharian B, Inamdar S, Fasanella K, Nasr J, Chennat J, Das R, DeWitt J, Easler JJ, Bick B, Singh H, Fairley KJ, Sarkaria S, Sawas T, Skef W, Slivka A, Tavakkoli A, Thakkar S, Kim V, Vanderveldt HD, Richardson A, Wallace MB, Brahmbhatt B, Engels M, Gabbert C, Dugum M, El-Dika S, Bhat Y, Ramrakhiani S, Bakis G, Rolshud D, Millspaugh G, Tielleman T, Schmidt C, Mansour J, Marsh W, Ongchin M, Centeno B, Monaco SE, Ohori NP, Lajara S, Thompson ED, Hruban RH, Bell PD, Smith K, Permuth JB, Vandenbussche C, Ernst W, Grupillo M, Kaya C, Hogg M, He J, Wolfgang CL, Lee KK, Zeh H, Zureikat A, Nikiforova MN, and Singhi AD
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- Humans, Retrospective Studies, Prospective Studies, High-Throughput Nucleotide Sequencing, Endoscopic Ultrasound-Guided Fine Needle Aspiration, Genomics, Mitogen-Activated Protein Kinases genetics, Cystadenoma, Serous diagnosis, Pancreatic Neoplasms diagnosis, Pancreatic Neoplasms genetics, Pancreatic Neoplasms surgery, Pancreatic Cyst diagnosis, Pancreatic Cyst genetics, Pancreatic Cyst therapy
- Abstract
Background & Aims: Next-generation sequencing (NGS) of pancreatic cyst fluid is a useful adjunct in the assessment of patients with pancreatic cyst. However, previous studies have been retrospective or single institutional experiences. The aim of this study was to prospectively evaluate NGS on a multi-institutional cohort of patients with pancreatic cyst in real time., Methods: The performance of a 22-gene NGS panel (PancreaSeq) was first retrospectively confirmed and then within a 2-year timeframe, PancreaSeq testing was prospectively used to evaluate endoscopic ultrasound-guided fine-needle aspiration pancreatic cyst fluid from 31 institutions. PancreaSeq results were correlated with endoscopic ultrasound findings, ancillary studies, current pancreatic cyst guidelines, follow-up, and expanded testing (Oncomine) of postoperative specimens., Results: Among 1933 PCs prospectively tested, 1887 (98%) specimens from 1832 patients were satisfactory for PancreaSeq testing. Follow-up was available for 1216 (66%) patients (median, 23 months). Based on 251 (21%) patients with surgical pathology, mitogen-activated protein kinase/GNAS mutations had 90% sensitivity and 100% specificity for a mucinous cyst (positive predictive value [PPV], 100%; negative predictive value [NPV], 77%). On exclusion of low-level variants, the combination of mitogen-activated protein kinase/GNAS and TP53/SMAD4/CTNNB1/mammalian target of rapamycin alterations had 88% sensitivity and 98% specificity for advanced neoplasia (PPV, 97%; NPV, 93%). Inclusion of cytopathologic evaluation to PancreaSeq testing improved the sensitivity to 93% and maintained a high specificity of 95% (PPV, 92%; NPV, 95%). In comparison, other modalities and current pancreatic cyst guidelines, such as the American Gastroenterology Association and International Association of Pancreatology/Fukuoka guidelines, show inferior diagnostic performance. The sensitivities and specificities of VHL and MEN1/loss of heterozygosity alterations were 71% and 100% for serous cystadenomas (PPV, 100%; NPV, 98%), and 68% and 98% for pancreatic neuroendocrine tumors (PPV, 85%; NPV, 95%), respectively. On follow-up, serous cystadenomas with TP53/TERT mutations exhibited interval growth, whereas pancreatic neuroendocrine tumors with loss of heterozygosity of ≥3 genes tended to have distant metastasis. None of the 965 patients who did not undergo surgery developed malignancy. Postoperative Oncomine testing identified mucinous cysts with BRAF fusions and ERBB2 amplification, and advanced neoplasia with CDKN2A alterations., Conclusions: PancreaSeq was not only sensitive and specific for various pancreatic cyst types and advanced neoplasia arising from mucinous cysts, but also reveals the diversity of genomic alterations seen in pancreatic cysts and their clinical significance., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2023
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34. Early Renal Recovery after the First Flare of Pauci-Immune Glomerulonephritis.
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Zaworski J, Gnemmi V, Bataille P, Hachulla E, Glowacki F, Gibier JB, Daroux M, Ratsimbazafy A, Bitton L, Humez S, Guincestre T, Béhal H, Azar R, Hoffmann M, Cardon G, Bourdon F, Lemoine C, Auxenfant E, Copin MC, Vandenbussche C, and Quéméneur T
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- Antibodies, Antineutrophil Cytoplasmic, Female, Humans, Kidney, Male, Retrospective Studies, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis, Glomerulonephritis diagnosis
- Abstract
Introduction: Renal involvement is a severe manifestation of antineutrophil cytoplasmic antibody-associated vasculitis. Patients often progress to end-stage renal disease. The potential for renal recovery after the first flare has seldom been studied. Our objectives were to describe the evolution of the estimated glomerular filtration rate (eGFR) and identify factors associated with the change in the eGFR between diagnosis and the follow-up at 3 months (ΔeGFRM0-M3)., Methods: This was a retrospective study over the period 2003-2018 of incident patients in the Nord-Pas-de-Calais (France). The primary outcome was the ΔeGFRM0-M3., Results: One hundred and seventy-seven patients were included. The eGFR at 3 months was significantly higher than at diagnosis (mean ± standard deviation, 40 ± 24 vs. 28 ± 26 mL/min/1.73 m2, p < 0.001), with a ΔeGFRM0-M3 of 12 ± 19 mL/min/1.73 m2. The eGFR at 12 months was higher than at 3 months (44 ± 13 vs. 40 ± 24 mL/min/1.73 m2, p = 0.003). The factors significantly associated with the ΔeGFRM0-M3 in multivariate analysis were the percentage of cellular crescents and neurological involvement. The mean increase in the eGFR was 2.90 ± 0.06 mL/min/1.73 m2 for every 10-point gain in the percentage of cellular crescents., Conclusions: Early renal recovery after the first flare of pauci-immune glomerulonephritis occurred mainly in the first 3 months of treatment. The percentage of cellular crescents was the main independent predictor of early renal recovery., (© 2022 S. Karger AG, Basel.)
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- 2022
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35. Reappraisal of Renal Arteritis in ANCA-associated Vasculitis: Clinical Characteristics, Pathology, and Outcome.
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Boudhabhay I, Delestre F, Coutance G, Gnemmi V, Quemeneur T, Vandenbussche C, Lazareth H, Canaud G, Tricot L, Gosset C, Hummel A, Terrier B, Rabant M, van Daalen EE, Wester Trejo MAC, Bajema IM, Karras A, and Duong Van Huyen JP
- Subjects
- Aged, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis mortality, Arteritis mortality, Disease-Free Survival, Female, France, Humans, Male, Middle Aged, Prognosis, Retrospective Studies, Risk Factors, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis complications, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis diagnosis, Arteritis complications, Arteritis diagnosis, Kidney Failure, Chronic epidemiology, Renal Artery
- Abstract
Background: Renal involvement in ANCA-associated vasculitis (AAV) is associated with poor outcomes. The clinical significance of arteritis of the small kidney arteries has not been evaluated in detail., Methods: In a multicenter cohort of patients with AAV and renal involvement, we sought to describe the clinicopathologic characteristics of patients with AAV who had renal arteritis at diagnosis, and to retrospectively analyze their prognostic value., Results: We included 251 patients diagnosed with AAV and renal involvement between 2000 and 2019, including 34 patients (13.5%) with arteritis. Patients with AAV-associated arteritis were older, and had a more pronounced inflammatory syndrome compared with patients without arteritis; they also had significantly lower renal survival ( P =0.01). In multivariable analysis, the ANCA renal risk score, age at diagnosis, history of diabetes mellitus, and arteritis on index kidney biopsy were independently associated with ESKD. The addition of the arteritis status significantly improved the discrimination of the ANCA renal risk score, with a concordance index (C-index) of 0.77 for the ANCA renal risk score alone, versus a C-index of 0.80 for the ANCA renal risk score plus arteritis status ( P =0.008); ESKD-free survival was significantly worse for patients with an arteritis involving small arteries who were classified as having low or moderate risk, according to the ANCA renal risk score. In two external validation cohorts, we confirmed the incidence and phenotype of this AAV subtype., Conclusions: Our findings suggest AAV with renal arteritis represents a different subtype of AAV with specific clinical and histologic characteristics. The prognostic contribution of the arteritis status remains to be prospectively confirmed., (Copyright © 2021 by the American Society of Nephrology.)
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- 2021
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36. Automated and rapid detection of cancer in suspicious axillary lymph nodes in patients with breast cancer.
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Li J, Downs BM, Cope LM, Fackler MJ, Zhang X, Song CG, VandenBussche C, Zhang K, Han Y, Liu Y, Tulac S, Venkatesan N, de Guzman T, Chen C, Lai EW, Yuan J, and Sukumar S
- Abstract
Preoperative staging of suspicious axillary lymph nodes (ALNs) allows patients to be triaged to ALN dissection or to sentinel lymph node biopsy (SLNB). Ultrasound-guided fine needle aspiration (FNA) and cytology of ALN is moderately sensitive but its clinical utility relies heavily on the cytologist's experience. We proposed that the 5-h automated GeneXpert system-based prototype breast cancer detection assay (BCDA) that quantitatively measures DNA methylation in ten tumor-specific gene markers could provide a facile, accurate test for detecting cancer in FNA of enlarged lymph nodes. We validated the assay in ALN-FNA samples from a prospective study of patients (N = 230) undergoing SLNB. In a blinded analysis of 218 evaluable LN-FNAs from 108 malignant and 110 benign LNs by histology, BCDA displayed a sensitivity of 90.7% and specificity of 99.1%, achieving an area under the ROC curve, AUC of 0.958 (95% CI: 0.928-0.989; P < 0.0001). Next, we conducted a study of archival FNAs of ipsilateral palpable LNs (malignant, N = 72, benign, N = 53 by cytology) collected in the outpatient setting prior to neoadjuvant chemotherapy (NAC). Using the ROC-threshold determined in the prospective study, compared to cytology, BCDA achieved a sensitivity of 94.4% and a specificity of 92.5% with a ROC-AUC = 0.977 (95% CI: 0.953-1.000; P < 0.0001). Our study shows that the automated assay detects cancer in suspicious lymph nodes with a high level of accuracy within 5 h. This cancer detection assay, scalable for analysis to scores of LN FNAs, could assist in determining eligibility of patients to different treatment regimens.
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- 2021
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37. Staphylococcus capitis isolated from bloodstream infections: a nationwide 3-month survey in 38 neonatal intensive care units.
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Decalonne M, Dos Santos S, Gimenes R, Goube F, Abadie G, Aberrane S, Ambrogi V, Baron R, Barthelemy P, Bauvin I, Belmonte O, Benabid E, Ammar RB, Yahia SBH, Berrouane Y, Berthelot P, Beuchee A, Bille E, Bolot P, Bordes-Couecou S, Bouissou A, Bourdon S, Bourgeois-Nicolaos N, Boyer S, Cattoen C, Cattoir V, Chaplain C, Chatelet C, Claudinon A, Chautemps N, Cormier H, Coroller-Bec C, Cotte B, De Chillaz C, Dauwalder O, Davy A, Delorme M, Demasure M, Desfrere L, Drancourt M, Dupin C, Faraut-Derouin V, Florentin A, Forget V, Fortineau N, Foucan T, Frange P, Gambarotto K, Gascoin G, Gibert L, Gilquin J, Glanard A, Grando J, Gravet A, Guinard J, Hery-Arnaud G, Huart C, Idri N, Jellimann JM, Join-Lambert O, Joron S, Jouvencel P, Kempf M, Ketterer-Martinon S, Khecharem M, Klosowski S, Labbe F, Lacazette A, Lapeyre F, Larche J, Larroude P, Le Pourhiennec A, Le Sache N, Ledru S, Lefebvre A, Legeay C, Lemann F, Lesteven C, Levast-Raffin M, Leyssene D, Ligi I, Lozniewski A, Lureau P, Mallaval FO, Malpote E, Marret S, Martres P, Menard G, Menvielle L, Mereghetti L, Merle V, Minery P, Morange V, Mourdie J, Muggeo A, Nakhleh J, Noulard MN, Olive C, Patural H, Penn P, Petitfrere M, Pozetto B, Riviere B, Robine A, Ceschin CR, Ruimy R, Siali A, Soive S, Slimani S, Trentesaux AS, Trivier D, Vandenbussche C, Villeneuve L, Werner E, Le Vu S, and Van Der Mee-Marquet N
- Subjects
- Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Catheter-Related Infections drug therapy, Catheter-Related Infections epidemiology, Catheter-Related Infections etiology, Drug Resistance, Multiple, Bacterial, Female, France epidemiology, Humans, Infant, Newborn, Infant, Premature, Intensive Care Units, Neonatal, Male, Sepsis drug therapy, Sepsis etiology, Staphylococcal Infections drug therapy, Staphylococcal Infections etiology, Staphylococcus capitis drug effects, Sepsis epidemiology, Staphylococcal Infections epidemiology, Staphylococcus capitis isolation & purification
- Abstract
To increase the knowledge about S. capitis in the neonatal setting, we conducted a nationwide 3-month survey in 38 neonatal intensive care units (NICUs) covering 56.6% of French NICU beds. We demonstrated 14.2% of S. capitis BSI (S.capBSI) among nosocomial BSIs. S.capBSI incidence rate was 0.59 per 1000 patient-days. A total of 55.0% of the S.capBSIs were late onset catheter-related BSIs. The S. capitis strains infected preterm babies (median gestational age 26 weeks, median birth weight 855 g). They were resistant to methicillin and aminoglycosides and belonged to the NRCS-A clone. Evolution was favorable in all but one case, following vancomycin treatment.
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- 2020
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38. Tubulointerstitial damage and interstitial immune cell phenotypes are useful predictors for renal survival and relapse in antineutrophil cytoplasmic antibody-associated vasculitis.
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Bitton L, Vandenbussche C, Wayolle N, Gibier JB, Cordonnier C, Verine J, Humez S, Bataille P, Lenain R, Ramdane N, Azar R, Mac Namara E, Hatron PY, Maurage CA, Perrais M, Frimat M, Vanhille P, Glowacki F, Buob D, Copin MC, Quéméneur T, and Gnemmi V
- Subjects
- Antibodies, Antineutrophil Cytoplasmic, Humans, Kidney pathology, Kidney Tubules immunology, Kidney Tubules pathology, Phenotype, Recurrence, Retrospective Studies, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis immunology, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis pathology, Kidney Failure, Chronic immunology, Kidney Failure, Chronic pathology
- Abstract
The aims of this study were to determine whether tubulointerstitial damage in the form of interstitial fibrosis/tubular atrophy and total interstitial inflammation predicted progression to end stage renal disease (ESRD) and/or renal relapse (RR) in patients with antineutrophil cytoplasmic antibody-associated vasculitis (AAV). One hundred thirteen patients with AAV from six French centers with an index biopsy performed between 2003 and 2013 were included. Histological assessments using the AAV glomerular classification and the kidney allograft Banff classification were performed on pathological review. Biopsy tissues were also investigated by CD3, CD20, CD68, CD163, FOXP3 and RORγt immunohistochemical staining. Competing risks models were calculated. Of the 113 patients, 26 (23.0%) died during follow-up and 29 (25.6%) developed ESRD. Among the 94 patients who achieved remission by the end of induction therapy without developing ESRD, 26 (27.6%) experienced RR. The two independent prognostic factors for ESRD were the estimated glomerular filtration rate at presentation (HR 0.35; 95% CI 0.23-0.51; P < 0.0001) and IF/TA > 25% (HR 2.27; 95% CI 1.18-4.37; P = 0.014). When the distribution of interstitial immune cell phenotypes was included in a second multivariable model, the organization of lymphocytic infiltrates was also an independent predictor of ESRD (HR 2.86; 95% CI 1.35-6.1, P = 0.006). The independent risk factors for RR were a higher CD3/CD20 ratio (HR 1.39; 95% CI 1.05-1.85; P = 0.02) and the presence of RORγt positive cells (HR 2.70; 95% CI 1.11-6.54; P = 0.02). Our results highlight the prognostic value of initial histological evaluations in AAV. Measurements of tubulointerstitial damage and interstitial immune cell phenotype distributions should be considered to improve risk assessments for ESRD and RR.
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- 2020
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39. Lower Genital Tract Dysplasia in Female Solid Organ Transplant Recipients.
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Thimm MA, Rositch AF, VandenBussche C, McDonald L, Garonzik Wang JM, and Levinson K
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- Adult, Early Detection of Cancer methods, Female, Genital Neoplasms, Female diagnosis, Genital Neoplasms, Female etiology, Humans, Hydroxychloroquine adverse effects, Incidence, Logistic Models, Middle Aged, Odds Ratio, Postoperative Complications diagnosis, Postoperative Complications etiology, Precancerous Conditions diagnosis, Precancerous Conditions etiology, Retrospective Studies, Risk Factors, Uterine Cervical Dysplasia diagnosis, Uterine Cervical Dysplasia epidemiology, Uterine Cervical Dysplasia etiology, Genital Neoplasms, Female epidemiology, Organ Transplantation adverse effects, Postoperative Complications epidemiology, Precancerous Conditions epidemiology
- Abstract
Objective: To examine the incidence of lower genital tract dysplasia in women after solid organ transplantation, to evaluate risk factors associated with development of dysplasia, and to assess the timeline of disease development., Methods: This was a retrospective study of female patients who underwent solid organ transplantation at a large-volume tertiary care center between 2000 and 2015. Demographic and clinicopathologic factors were extracted from electronic medical records. Cumulative incidence of lower genital tract dysplasia was calculated, and univariate and multivariable logistic regression were performed to identify risk factors for the development of dysplasia., Results: Among 394 female solid organ transplant recipients, the median age was 41 years (interquartile range 29-53). Forty-seven (11.9%; 95% CI 8.8-15.9%) women developed lower genital tract dysplasia over a median follow-up of 7.8 years (interquartile range 4.6-12.9). Thirty-eight (9.6%) developed cervical intraepithelial neoplasia (CIN), with 14 (3.6%) diagnosed with CIN 2 or worse (one was cervical carcinoma). Nineteen (4.8%) developed noncervical lower genital tract dysplasia, including vulvar, vaginal, or anal dysplasia, with 13 (3.3%) diagnosed with high-grade dysplasia or worse (five were lower genital tract carcinoma [three anal, one vulvar, and one vaginal]). Ten (2.5%) developed both cervical and noncervical lower genital tract dysplasia. Black race was significantly associated with developing dysplasia (odds ratio [OR] 2.86; 95% CI 1.33-6.13) as was hydroxychloroquine use (OR 5.95; 95% CI 1.96-18.09). High-grade cervical dysplasia was diagnosed at a median interval of 3.18 years after transplant; noncervical high-grade lower genital tract dysplasia was diagnosed at a median interval of 3.94 years., Conclusions: One in eight transplant recipients developed lower genital tract dysplasia and approximately half were high-grade dysplasia or cancer. Black race and hydroxychloroquine use were associated with an increased risk of dysplasia. Yearly cervical screening and comprehensive lower genital examination beyond the cervix is indicated in this population.
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- 2019
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40. 90Y-TheraSpheres: The New Look of Yttrium-90.
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Arnold CA, Pezhouh MK, Lam-Himlin D, Pittman ME, VandenBussche C, and Voltaggio L
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- Adult, Aged, Carcinoma, Hepatocellular diagnostic imaging, Carcinoma, Hepatocellular pathology, Carcinoma, Hepatocellular surgery, Female, Hepatectomy, Humans, Liver Neoplasms diagnostic imaging, Liver Neoplasms pathology, Liver Neoplasms surgery, Male, Microscopy, Polarization, Microspheres, Middle Aged, Particle Size, Radiopharmaceuticals adverse effects, Treatment Outcome, Yttrium Radioisotopes adverse effects, Carcinoma, Hepatocellular radiotherapy, Liver Neoplasms radiotherapy, Radiopharmaceuticals administration & dosage, Yttrium Radioisotopes administration & dosage
- Abstract
Selective internal radiation therapy with Y-TheraSphere or Y-SIRSphere is used in the treatment of unresectable hepatic malignancies. To the best of our knowledge, this is the first Y-TheraSpheres series. BTG International Canada Inc. provided nonradiated microspheres from the Nordion manufacturer. The histologic processed microspheres were colorless, refractile, polarizable, 20 to 30 μm in diameter, and an occasional internal bulls'-eye seen with the condenser out and an internal cross seen with polarized light. Identical microspheres were identified in 15 hepatectomy specimens from four centers between February 2016 and March 2018. The patients were usually male (male=10, female=5) with a mean age of 59 years. All patients had a prior diagnosis of hepatocellular carcinoma (HCC) and documented Y-TheraSphere (mean duration from last deployment=32 wk). All surgical pathology specimens in these 15 patients were reviewed, but the microspheres were only identified in the hepatectomy specimens. During manuscript preparation, one case of Y-TheraSpheres gastritis was prospectively identified from a separate patient with a history of HCC and Y-TheraSpheres. In conclusion, recognition of Y-TheraSpheres is important so that one may consider the possibility of a nearby malignancy and or establish the cause of the background inflammatory or radiation-related injury. These structures can be easy to miss because the subtle morphology is distinct from previously reported Y-SIRSphere. Clues to the diagnosis include a history of HCC and background radiation change. We report the characteristic morphology as microspheres that overlap in size with Y-SIRSphere, but can be differentiated based on Y-TheraSpheres' colorless appearance with occasional internal bulls'-eyes with the condenser out and an internal cross with polarized light.
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- 2019
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41. Prognostic Value of Microscopic Hematuria after Induction of Remission in Antineutrophil Cytoplasmic Antibodies-Associated Vasculitis.
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Vandenbussche C, Bitton L, Bataille P, Glowacki F, Azar R, Hatron PY, Macnamara E, Gheerbrant JD, Cardon G, Hoffmann M, Auxenfants E, Gnemmi V, and Quéméneur T
- Subjects
- Adult, Aged, Aged, 80 and over, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis complications, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis immunology, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis mortality, Disease Progression, Female, Follow-Up Studies, France epidemiology, Glomerulonephritis complications, Glomerulonephritis immunology, Glomerulonephritis mortality, Hematuria diagnosis, Hematuria immunology, Hematuria urine, Humans, Kaplan-Meier Estimate, Kidney Failure, Chronic immunology, Kidney Failure, Chronic pathology, Male, Middle Aged, Prognosis, Recurrence, Registries statistics & numerical data, Remission Induction methods, Retrospective Studies, Survival Rate, Time Factors, Treatment Outcome, Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis drug therapy, Glomerulonephritis drug therapy, Hematuria epidemiology, Immunosuppressive Agents therapeutic use, Kidney Failure, Chronic epidemiology
- Abstract
Background: Pauci-immune glomerulonephritis (PIGN) is a major prognostic factor in antineutrophil cytoplasmic antibodies-associated vasculitis (AAV). Renal remission is usually defined as improvement or stabilization of serum creatinine and proteinuria levels but the significance of hematuria is unclear. We evaluated the prognostic value of microscopic hematuria in patients in remission from a first flare of PIGN., Methods: A multicenter retrospective study was conducted of all patients with histologically proven PIGN in northern France who presented a first renal flare of AAV between 2003 and 2013. All patients received conventional induction treatment and were considered in remission. Two groups were defined by the presence (H+) or absence (H-) of hematuria (dipstick 1+ and/or cytology ≥10,000 erythrocytes/mL). The primary outcome measure was the occurrence of renal relapse (RR) and/or end-stage renal disease (ESRD)., Results: Eighty-six patients were included: 41 (48%) had hematuria at remission. The median follow-up time was 44 ± 34 months. There was no significant difference between the groups in terms of the primary endpoint or the number of RR. However, the survival rate without RR was significantly lower in the H+ group (p = 0.002). In multivariate analysis, risk factors for RR were hematuria at remission for relapses within 44 months (hazard ratio [HR] 4.15; 95% CI 1.15-15.01; p = 0.03) and the duration of maintenance immunosuppressive therapy (HR 0.96 per additional month; 95% CI 0.94-0.99; p = 0.002)., Conclusion: Hematuria at remission after a first PIGN flare was not associated with ESRD but with the occurrence of RR within 44 months of remission., (© 2019 S. Karger AG, Basel.)
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- 2019
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42. Correction: Non-invasive detection of urothelial cancer through the analysis of driver gene mutations and aneuploidy.
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Springer SU, Chen CH, Rodriguez Pena MDC, Li L, Douville C, Wang Y, Cohen JD, Taheri D, Silliman N, Schaefer J, Ptak J, Dobbyn L, Papoli M, Kinde I, Afsari B, Tregnago AC, Bezerra SM, VandenBussche C, Fujita K, Ertoy D, Cunha IW, Yu L, Bivalacqua TJ, Grollman AP, Diaz LA, Karchin R, Danilova L, Huang CY, Shun CT, Turesky RJ, Yun BH, Rosenquist TA, Pu YS, Hruban RH, Tomasetti C, Papadopoulos N, Kinzler KW, Vogelstein B, Dickman KG, and Netto GJ
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- 2018
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43. Tacrolimus-induced nephrotoxicity in mice is associated with microRNA deregulation.
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Vandenbussche C, Van der Hauwaert C, Dewaeles E, Franczak J, Hennino MF, Gnemmi V, Savary G, Tavernier Q, Nottet N, Paquet A, Perrais M, Blum D, Mari B, Pottier N, Glowacki F, and Cauffiez C
- Subjects
- Animals, Cells, Cultured, Fibrosis, Humans, Kidney metabolism, Kidney pathology, Mice, Transcriptome drug effects, Up-Regulation, Immunosuppressive Agents toxicity, Kidney drug effects, MicroRNAs metabolism, Tacrolimus toxicity
- Abstract
Although Tacrolimus is an immunosuppressive drug widely used in renal transplantation, its chronic use paradoxically induces nephrotoxic effects, in particular renal fibrosis, which is responsible for chronic allograft dysfunction and represents a major prognostic factor of allograft survival. As molecular pathways and mechanisms involved in Tacrolimus-induced fibrogenic response are poorly elucidated, we assessed whether miRNAs are involved in the nephrotoxic effects mediated by Tacrolimus. Treatment of CD-1 mice with Tacrolimus (1 mg/kg/d for 28 days) resulted in kidney injury and was associated with alteration of a gene expression signature associated with cellular stress, fibrosis and inflammation. Tacrolimus also affected renal miRNA expression, including miRNAs previously involved in fibrotic and inflammatory processes as "fibromirs" such as miR-21-5p, miR-199a-5p and miR-214-3p. In agreement with in vivo data, Renal Proximal Tubular Epithelial cells exposed to Tacrolimus (25 and 50 µM) showed upregulation of miR-21-5p and the concomitant induction of epithelial phenotypic changes, inflammation and oxidative stress. In conclusion, this study suggests for the first time that miRNAs, especially fibromiRs, participate to Tacrolimus-induced nephrotoxic effects. Therefore, targeting miRNAs may be a new therapeutic option to counteract Tacrolimus deleterious effects on kidney.
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- 2018
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44. Non-invasive detection of urothelial cancer through the analysis of driver gene mutations and aneuploidy.
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Springer SU, Chen CH, Rodriguez Pena MDC, Li L, Douville C, Wang Y, Cohen JD, Taheri D, Silliman N, Schaefer J, Ptak J, Dobbyn L, Papoli M, Kinde I, Afsari B, Tregnago AC, Bezerra SM, VandenBussche C, Fujita K, Ertoy D, Cunha IW, Yu L, Bivalacqua TJ, Grollman AP, Diaz LA, Karchin R, Danilova L, Huang CY, Shun CT, Turesky RJ, Yun BH, Rosenquist TA, Pu YS, Hruban RH, Tomasetti C, Papadopoulos N, Kinzler KW, Vogelstein B, Dickman KG, and Netto GJ
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Carcinoma, Transitional Cell genetics, Carcinoma, Transitional Cell urine, Child, Child, Preschool, Female, Genetic Testing methods, Humans, Male, Middle Aged, Sensitivity and Specificity, Telomerase genetics, Urinary Bladder Neoplasms genetics, Urinary Bladder Neoplasms urine, Young Adult, Aneuploidy, Carcinoma, Transitional Cell diagnosis, Early Detection of Cancer methods, Mutation, Urinary Bladder Neoplasms diagnosis
- Abstract
Current non-invasive approaches for detection of urothelial cancers are suboptimal. We developed a test to detect urothelial neoplasms using DNA recovered from cells shed into urine. UroSEEK incorporates massive parallel sequencing assays for mutations in 11 genes and copy number changes on 39 chromosome arms. In 570 patients at risk for bladder cancer (BC), UroSEEK was positive in 83% of those who developed BC. Combined with cytology, UroSEEK detected 95% of patients who developed BC. Of 56 patients with upper tract urothelial cancer, 75% tested positive by UroSEEK, including 79% of those with non-invasive tumors. UroSEEK detected genetic abnormalities in 68% of urines obtained from BC patients under surveillance who demonstrated clinical evidence of recurrence. The advantages of UroSEEK over cytology were evident in low-grade BCs; UroSEEK detected 67% of cases whereas cytology detected none. These results establish the foundation for a new non-invasive approach for detection of urothelial cancer., Competing Interests: SS, CC, MR, LL, CD, YW, JC, DT, NS, JS, JP, LD, MP, IK, BA, AT, SB, CV, KF, DE, IC, LY, TB, AG, LD, RK, LD, CH, CS, RT, BY, TR, YP, RH, CT, KD, GN No competing interests declared, NP Founder of Personal Genome Diagnostics and PapGene and advises Sysmex-Inostics. These companies and others have licensed technologies from Johns Hopkins, of which BV, KK, and NP are inventors on a patent (U.S. 20140227705 A1) and receive royalties. The terms of these arrangements are managed by the university in accordance with its conflict of interest policies. Luis A Diaz: Member of the board of directors of Personal Genome Diagnostics (PGDx) and Jounce Therapeutics. LAD holds equity in PapGene, Personal Genome Diagnostics (PGDx) and Phoremost. He is a paid consultant for Merck, PGDx and Phoremost. LAD is an inventor of licensed intellectual property related to technology for ctDNA analyses and mismatch repair deficiency for diagnosis and therapy from Johns Hopkins University. These licenses and relationships are associated with equity or royalty payments to LAD. The terms of all these arrangements are being managed by Johns Hopkins and Memorial Sloan Kettering in accordance with their conflict of interest policies. In addition, in the past 5 years, LAD has participated as a paid consultant for one-time engagements with Caris, Lyndra, Genocea Biosciences, Illumina and Cell Design Labs. KK Ken W Kinzler: Founder of Personal Genome Diagnostics and PapGene and advises Sysmex-Inostics. These companies and others have licensed technologies from Johns Hopkins, of which BV, KK, and NP are inventors on a patent (U.S. 20140227705 A1) and receive royalties. The terms of these arrangements are managed by the university in accordance with its conflict of interest policies. BV Bert Vogelstein: Founder of Personal Genome Diagnostics and PapGene and advises Sysmex-Inostics. These companies and others have licensed technologies from Johns Hopkins, of which BV, KK, and NP are inventors on a patent (U.S. 20140227705 A1) and receive royalties. The terms of these arrangements are managed by the university in accordance with its conflict of interest policies., (© 2018, Springer et al.)
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- 2018
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45. Preinvasive and Invasive Cervical Adenocarcinoma: Preceding Low-Risk or Negative Pap Result Increases Time to Diagnosis.
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Moukarzel LA, Angarita AM, VandenBussche C, Rositch A, Thompson CB, Fader AN, and Levinson K
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- Adolescent, Adult, Aged, Female, Humans, Middle Aged, Retrospective Studies, Time Factors, Young Adult, Adenocarcinoma diagnosis, Early Detection of Cancer methods, Papanicolaou Test, Papillomavirus Infections diagnosis, Uterine Cervical Neoplasms diagnosis, Vaginal Smears
- Abstract
Objectives: The aims of the study were to investigate screening histories of women with adenocarcinoma in situ (AIS) and adenocarcinoma (AdCa) of the cervix and to further evaluate screening for glandular disease., Materials and Methods: Screening histories were retrospectively collected for patients with AIS or AdCa at a single large-volume academic institution from 2005 to 2015. Fisher exact and Wilcoxon rank sum tests were used to compare AIS with AdCa patient characteristics, distribution of preceding Pap (i.e., trigger Pap) results, and high-risk human papillomavirus testing. The association between Pap result and time to diagnosis was evaluated., Results: Eighty-seven cases, 50 AIS and 37 AdCa, met study criteria; median age was 31 and 43 years, respectively. Among the AIS cohort, 52.0% had a negative or low-risk trigger Pap result versus 24.3% of those with AdCa (p = .001). The time to diagnosis of AIS ranged from 8.4 to 18.8 weeks for those with high- versus low-risk or negative trigger Pap results, respectively (p = .002). The time to diagnosis of AdCa ranged from 14.6 to 44.7 weeks for those with high- versus low-risk or negative trigger Pap results, respectively (p = .003). Among those with high-risk human papillomavirus testing, 89.7% tested positive at the time of trigger Pap with 100% positivity among those with low-risk or negative trigger Pap results., Conclusions: Cervical AIS and AdCa affect many young nulliparous women and commonly preceded by low-risk or negative Pap testing. The interval to diagnosis increases with low-risk and negative Pap results, and therefore, further investigation into optimal screening for glandular lesions is needed.
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- 2017
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46. Epithelioid hemangioendothelioma in pleural effusion.
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Sayah M, VandenBussche C, and Maleki Z
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- Adult, Exudates and Transudates, Female, Humans, Pleural Effusion pathology, Young Adult, Hemangioendothelioma, Epithelioid diagnosis, Hemangioendothelioma, Epithelioid pathology, Liver pathology, Lung pathology
- Abstract
Epithelioid hemangioendothelioma (EHE) is a rare mesenchymal tumor of vascular origin with variable morphological features and unpredictable biological behavior. EHE occasionally involves the pleural fluid. However, the cytomorphology of EHE found in pleural fluid has not been well characterized in the literature. Herein we describe a case of EHE, initially presenting as multiple liver lesions plus several small nodular densities at the bases of the lungs followed by bilateral pleural effusions., (© 2015 Wiley Periodicals, Inc.)
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- 2015
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47. Involvement of epigenetics and EMT-related miRNA in arsenic-induced neoplastic transformation and their potential clinical use.
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Michailidi C, Hayashi M, Datta S, Sen T, Zenner K, Oladeru O, Brait M, Izumchenko E, Baras A, VandenBussche C, Argos M, Bivalacqua TJ, Ahsan H, Hahn NM, Netto GJ, Sidransky D, and Hoque MO
- Subjects
- Adult, Aged, Aged, 80 and over, Apoptosis, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Blotting, Western, Case-Control Studies, Cell Movement, Cell Proliferation, Cell Transformation, Neoplastic chemically induced, Cell Transformation, Neoplastic genetics, Cells, Cultured, Cohort Studies, DNA Methylation, Female, Gene Expression Regulation, Neoplastic drug effects, Humans, Immunoenzyme Techniques, Male, MicroRNAs genetics, Middle Aged, Neoplasm Invasiveness, PTEN Phosphohydrolase genetics, PTEN Phosphohydrolase metabolism, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, TOR Serine-Threonine Kinases genetics, TOR Serine-Threonine Kinases metabolism, Urinary Bladder drug effects, Urinary Bladder metabolism, Urinary Bladder Neoplasms drug therapy, Urinary Bladder Neoplasms genetics, Young Adult, Arsenic adverse effects, Cell Transformation, Neoplastic pathology, Epigenesis, Genetic genetics, Epithelial-Mesenchymal Transition, MicroRNAs analysis, Urinary Bladder pathology, Urinary Bladder Neoplasms pathology
- Abstract
Exposure to toxicants leads to cumulative molecular changes that overtime increase a subject's risk of developing urothelial carcinoma. To assess the impact of arsenic exposure at a time progressive manner, we developed and characterized a cell culture model and tested a panel of miRNAs in urine samples from arsenic-exposed subjects, urothelial carcinoma patients, and controls. To prepare an in vitro model, we chronically exposed an immortalized normal human bladder cell line (HUC1) to arsenic. Growth of the HUC1 cells was increased in a time-dependent manner after arsenic treatment and cellular morphology was changed. In a soft agar assay, colonies were observed only in arsenic-treated cells, and the number of colonies gradually increased with longer periods of treatment. Similarly, invaded cells in an invasion assay were observed only in arsenic-treated cells. Withdrawal of arsenic treatment for 2.5 months did not reverse the tumorigenic properties of arsenic-treated cells. Western blot analysis demonstrated decreased PTEN and increased AKT and mTOR in arsenic-treated HUC1 cells. Levels of miR-200a, miR-200b, and miR-200c were downregulated in arsenic-exposed HUC1 cells by quantitative RT-PCR. Furthermore, in human urine, miR-200c and miR-205 were inversely associated with arsenic exposure (P = 0.005 and 0.009, respectively). Expression of miR-205 discriminated cancer cases from controls with high sensitivity and specificity (AUC = 0.845). Our study suggests that exposure to arsenic rapidly induces a multifaceted dedifferentiation program and miR-205 has potential to be used as a marker of arsenic exposure as well as a maker of early urothelial carcinoma detection., (©2015 American Association for Cancer Research.)
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- 2015
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48. Inexpensive telecytology solutions that use the Raspberry Pi and the iPhone.
- Author
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Dudas R, VandenBussche C, Baras A, Ali SZ, and Olson MT
- Abstract
Introduction: There is currently substantial interest in dynamic telecytology-the presentation of microscopic findings by live video feed to a cytopathologist at a remote location. However, the initial costs of a telecytology system can be high. We present several low-cost alternatives along with their performance characteristics., Materials and Methods: We tested 3 low-cost telecytology systems: a Raspberry Pi with a webcam, an iPhone 4S with FaceTime, and an iPhone 4S with a live streaming app. Costs, resolution capacities, and latency periods for image transmission were determined., Results: At $85.55, the Raspberry Pi system is the least expensive telecytology solution described to date. When the cost per megapixel of resolution is considered, the cost of a Raspberry Pi system is 120× less than the most expensive commercially available option and about 7-fold less than the iPhone-based alternatives. Latency periods were substantially lower for the iPhone systems: 2.5 ± 1 seconds for FaceTime and 2.8 ± 0.3 seconds for iPhone live streaming versus 6.6 ± 0.6 seconds for the Raspberry Pi system at comparable frame rates., Conclusions: This proof-of-principle study demonstrates that inexpensive telecytology systems are able to stream live video feeds of cytology slides from a microscope to a remote location at useable resolutions., (Copyright © 2014 American Society of Cytopathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
49. Simplified prediction rule for prognosis of patients with severe community-acquired pneumonia in ICUs.
- Author
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Leroy O, Devos P, Guery B, Georges H, Vandenbussche C, Coffinier C, Thévenin D, and Beaucaire G
- Subjects
- Community-Acquired Infections mortality, Female, Humans, Intensive Care Units, Male, Middle Aged, Multivariate Analysis, Prognosis, Retrospective Studies, Risk Factors, Survival Analysis, Pneumonia mortality
- Abstract
Study Objectives: To develop a simplified prognostic prediction rule for patients admitted to ICUs for severe community-acquired pneumonia (CAP)., Setting: Six ICUs in the north of France., Patients: Five hundred five patients admitted to ICUs over a 9-year period (from 1987 to 1995) for severe CAP., Interventions: Retrospective prognosis analysis and multivariate analysis using a credit scoring technique., Measurements: The primary outcome measure was ICU mortality., Results: Among the 505 patients, 472 were eligible for the prognosis study. The ICU mortality rate was 22.9%. Multivariate analysis identified, on the basis of the patient's medical history and initial examination on ICU admission, six independent predictors of mortality: age > or = 40 years, anticipated death within 5 years, nonaspiration pneumonia, chest radiograph involvement > 1 lobe, acute respiratory failure requiring mechanical ventilation, and septic shock. An initial risk score based on these factors classified patients into three risk classes of increasing mortality: 4% in class I, 25% in class II, and 60% in class III. Multivariate analysis of events occurring during ICU stay identified three independent predictors of mortality: hospital-acquired lower respiratory tract superinfections, nonspecific CAP-related complications, and sepsis-related complications. An adjustment risk score based on these factors was essential to accurately predict the final outcome of patients in the initial risk class II., Conclusions: As an aid to clinicians in stratifying the prognosis of patients with severe CAP, the simplified prediction rule used in this study could be useful for therapeutic decisions and appropriate care.
- Published
- 1999
- Full Text
- View/download PDF
50. Community-acquired pneumonia in the intensive care unit: epidemiological and prognosis data in older people.
- Author
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Leroy O, Bosquet C, Vandenbussche C, Coffinier C, Georges H, Guery B, Alfandari S, Thevenin D, and Beaucaire G
- Subjects
- Age Factors, Aged, Community-Acquired Infections mortality, Discriminant Analysis, Female, France, Humans, Male, Middle Aged, Multivariate Analysis, Prognosis, Prospective Studies, Retrospective Studies, Survival Analysis, Intensive Care Units statistics & numerical data, Pneumonia, Bacterial mortality
- Abstract
Objectives: To compare epidemiological data, etiology, and prognosis of severe community-acquired pneumonia (CAP) in the intensive care unit (ICU) according to age (< or > or = 65 years) and to determine prognostic factors of CAP in older people., Design: A retrospective (1987-1992) and prospective (1993-95) multicenter study., Setting: Six ICUs in the north of France., Patients: Five hundred five patients admitted to an ICU for severe CAP., Measurements: Patient characteristics were compared with regard to age. Prognosis of CAP in older patients was studied by stepwise discriminant analysis., Results: Two hundred seventy-eight patients (55%) were aged 65 years or older. Comparison of epidemiological data between older and younger patients revealed a higher prevalence of women (38% vs 29%), more severe underlying comorbidities (anticipated death within 5 years: 59% vs 26%), and more frequent chronic respiratory insufficiency (48% vs 33%) in the older patients. In this study group, 224 organisms were isolated from 172 patients (62%); those identified most frequently were Gram-negative bacilli (34%), S. pneumoniae (32%), and Staphylococcus sp. (19%). Compared with younger patients, no significant differences in bacteriological data were observed. However, crude and attributable mortality rates were significantly higher in the older patients (33% vs 21% and 30% vs 19%, respectively). Prognosis analysis identified four independent predictors of mortality in the older patients: initial septic shock (relative risk (RR) = 3), sepsis-related complications (RR = 4.3), hospital-acquired lower respiratory tract superinfections (RR = 2), and nonspecific pneumonia-related complications (RR = 2.8)., Conclusion: The bacterial etiology provides some approaches to empirical therapy for older patients with severe community-acquired pneumonia. In addition, the inappropriateness of withholding intensive care for reasons of age alone is emphasized.
- Published
- 1999
- Full Text
- View/download PDF
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