32 results on '"Takaichi Y"'
Search Results
2. Superconducting Transition in the $\beta$-Pyrochlore AOs$_2$O$_6$ (A=Cs, Rb, K) under Pressure
- Author
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Miyoshi, K., Takaichi, Y., Takamatsu, Y., Miura, M., and Takeuchi, J.
- Subjects
Condensed Matter - Superconductivity - Abstract
Pressure dependence of superconducting transition temperature $T_{\rm c}$ has been determined through the DC magnetic measurements under pressure up to $P$=10 GPa for $\beta$-pyrochlore oxides AOs$_2$O$_6$ with A=Cs ($T_{\rm c}$=3.3 K), Rb (6.3 K) and K (9.6 K). Both for A=Rb and Cs, $T_{\rm c}$ increases with increasing $P$ and shows a saturation at $T_{\rm cm}$$\sim$8.8 K, which is considered as the upper limit of $T_{\rm c}$ inherent in AOs$_2$O$_6$. In contrast, the $T_{\rm c}-P$ curve for KOs$_2$O$_6$ shows a sharp maximum of $\sim$10 K at $P$$\sim$0.5 GPa, and $T_{\rm c}$ is higher than $T_{\rm cm}$ for 0$\leq$$P$$\leq$1.5GPa, suggesting the enhanced superconductivity due to the rattling of K ions., Comment: 4 pages, 4 figures, to be published in J. Phys. Soc. Jpn. Vol. 77, No. 4 (2008)
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- 2008
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3. Thyroidal prelymphoma
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Matsubayashi, S., Tamai, H., Takaichi, Y., Morita, T., Matsuzuka, F., Suzuki, T., Kuma, K., and Nagataki, S.
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- 1988
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4. Evaluating GraphQL and REST API Services Performance in a Massive and Intensive Accessible Information System
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Armin Lawi, Benny L. E. Panggabean, and Takaichi Yoshida
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information systems ,middleware applications ,application programming interfaces (APIs) ,web services ,quality of services (QoS) ,performance evaluation ,Electronic computers. Computer science ,QA75.5-76.95 - Abstract
Currently, most middleware application developers have two choices when designing or implementing Application Programming Interface (API) services; i.e., they can either stick with Representational State Transfer (REST) or explore the emerging GraphQL technology. Although REST is widely regarded as the standard method for API development, GraphQL is believed to be revolutionary in overcoming the main drawbacks of REST, especially data-fetching issues. Nevertheless, doubts still remain, as there are no investigations with convincing results in evaluating the performance of the two services. This paper proposes a new research methodology to evaluate the performance of REST and GraphQL API services with two main ideas as novelties. The first novel method is the evaluation of the two services is performed on the real ongoing operation of the management information system, where massive and intensive query transactions take place on a complex database with many relationships. The second is the fair and independent performance evaluation results obtained by distributing client requests and synchronizing the service responses on the two virtually separated parallel execution paths for each API service, respectively. The performance evaluation was investigated using basic measures of QoS (Quality of Services), i.e., response time, throughput, CPU load, and memory usage. We use the term efficiency in comparing the evaluation results to capture differences in their performance measures. The statistical hypothesis parameters test using the two-tails paired t-test, and boxplot visualization was also given to confirm the significance of the comparison results. The results showed REST is still faster up to 50.50% in response time and 37.16% for throughput, while GraphQL is very efficient in resource utilization, i.e., 37.26% for CPU load and 39.74% for memory utilization. Therefore, GraphQL is the right choice when data requirements change frequently, and resource utilization is the most important consideration. REST is used when some data are frequently accessed and called by multiple requests.
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- 2021
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5. Thermal characterization of a polyethyleneglycol (PEG)-derivative induced vesicle fusion as revealed by high sensitivity differential scanning calorimetry
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Kodama, M., primary, Tsuchiya, S., additional, Nakayama, K., additional, Takaichi, Y., additional, Sakiyama, M., additional, Akiyoshi, K., additional, Tanaka, K., additional, and Sunamoto, J., additional
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- 1990
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6. Angiotensin-Converting Enzyme and Anorexia Nervosa.
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Matsubayashi, S., Tamai, H., Kobayashi, N., Takaichi, Y., Fukata, S., Hirota, Y., Kuma, K., Nakagawa, T., and Kumagai, L. F.
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- 1988
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7. Decreased thyroidal triiodothyronine secretion in patients with anorexia nervosa: influence of weight recovery
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Kiyohara, K, primary, Tamai, H, additional, Takaichi, Y, additional, Nakagawa, T, additional, and Kumagai, L F, additional
- Published
- 1989
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8. Decreased thyroidal triiodothyronine secretion in patients with anorexia nervosa: influence of weight recovery
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Nakagawa, T., Tamai, H., Kiyohara, K., Kumagai, L. F. Kumagai, and Takaichi, Y.
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ANOREXIA nervosa - Published
- 1989
9. Cytoplasmic aggregation of TDP43 and topographic correlation with tau and α-synuclein accumulation in the rTg4510 mouse model of tauopathy.
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Nakayama Y, Chambers JK, Takaichi Y, and Uchida K
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- Animals, Mice, Humans, Brain metabolism, Brain pathology, Phosphorylation, Neurons metabolism, Neurons pathology, alpha-Synuclein metabolism, alpha-Synuclein genetics, tau Proteins metabolism, tau Proteins genetics, Tauopathies pathology, Tauopathies metabolism, Tauopathies genetics, DNA-Binding Proteins metabolism, DNA-Binding Proteins genetics, Mice, Transgenic, Disease Models, Animal, Cytoplasm metabolism
- Abstract
In patients with TDP43 proteinopathy, phosphorylated TDP43 (p-TDP43) accumulates in the cytoplasm of neurons. The accumulation of p-TDP43 has also been reported in patients with tauopathy and α-synucleinopathy. We investigated spatiotemporal changes in p-TDP43 accumulation in the brains of rTg4510 mice that overexpressed human mutant tau (P301L) and exhibited hyperphosphorylated tau (hp-tau) and phosphorylated αSyn (p-αSyn) accumulation. Immunohistochemically, p-TDP43 aggregates were observed in the cytoplasm of neurons, which increased with age. A significant positive correlation was observed between the number of cells with p-TDP43 aggregates and hp-tau and p-αSyn aggregates. Suppression of the human mutant tau (P301L) expression by doxycycline treatment reduces the accumulation of p-TDP43, hp-tau, and p-αSyn. Proteinase K-resistant p-TDP43 aggregates were found in regions with high hp-tau, and p-αSyn accumulation. Western blotting of the sarkosyl-insoluble fraction revealed bands of monomeric TDP43 and p-TDP43. These results indicate that the accumulation of mouse p-TDP43 is associated with the accumulation of human mutant tau (P301L) in rTg4510 mouse brains. The accumulation of hp-tau and p-αSyn may promote sarkosyl-insoluble p-TDP43 aggregates that are resistant to proteinase K. The synergistic effects of tau, TDP43, and αSyn may be involved in the pathology of proteinopathies, leading to the accumulation of multiple abnormal proteins., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Association of Neuropathologists, Inc. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
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- 2024
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10. A novel monoclonal antibody generated by immunization with granular tau oligomers binds to tau aggregates at 423-430 amino acid sequence.
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Soeda Y, Hayashi E, Nakatani N, Ishigaki S, Takaichi Y, Tachibana T, Riku Y, Chambers JK, Koike R, Mohammad M, and Takashima A
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- Animals, Humans, Mice, Rats, Immunization, Female, Amino Acid Sequence, Epitopes immunology, Male, Aged, Protein Aggregates, Neurofibrillary Tangles metabolism, tau Proteins metabolism, tau Proteins immunology, tau Proteins chemistry, Antibodies, Monoclonal immunology, Alzheimer Disease metabolism, Alzheimer Disease immunology, Mice, Transgenic
- Abstract
Prior to the formation of amyloid fibrils, the pathological hallmark in tau-related neurodegenerative disease, tau monomers aggregate into a diverse range of oligomers. Granular tau oligomers, consisting of approximately 40 tau protein molecules, are present in the prefrontal cortex of patients at Braak stages I-II, preclinical stages of Alzheimer's disease (AD). Antibodies to granular tau oligomers as antigens have not been reported. Therefore, we generated new rat monoclonal antibodies by immunization with granular tau oligomers. Three antibodies from different hybridoma clones showed stronger immunoreactivity to granular tau oligomers and tau fibrils compared with monomeric tau. Of the three antibodies, 2D6-2C6 showed 3000-fold greater immunoreactivity in P301L-tau transgenic (rTg4510) mice than in non-transgenic mice, while MC1 antibody, which detects pathological conformations of tau, showed a 5.5-fold increase. These results suggest that 2D6-2C6 recognizes aggregates more specifically than MC1. In AD subjects, 2D6-2C6 recognized neurofibrillary tangles and pretangles, and co-localized within AT8-positive cells containing phosphorylated tau aggregates. The epitope of 2D6-2C6 is the 423-430 amino acid (AA) sequence of C-terminal regions. Taken together, a novel monoclonal antibody, 2D6-2C6, generated by immunization with granular tau oligomers binds to tau aggregates at the 423-430 AA sequence., (© 2024. The Author(s).)
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- 2024
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11. Path integration deficits are associated with phosphorylated tau accumulation in the entorhinal cortex.
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Koike R, Soeda Y, Kasai A, Fujioka Y, Ishigaki S, Yamanaka A, Takaichi Y, Chambers JK, Uchida K, Watanabe H, and Takashima A
- Abstract
Alzheimer's disease is a devastating disease that is accompanied by dementia, and its incidence increases with age. However, no interventions have exhibited clear therapeutic effects. We aimed to develop and characterize behavioural tasks that allow the earlier identification of signs preceding dementia that would facilitate the development of preventative and therapeutic interventions for Alzheimer's disease. To this end, we developed a 3D virtual reality task sensitive to the activity of grid cells in the entorhinal cortex, which is the region that first exhibits neurofibrillary tangles in Alzheimer's disease. We investigated path integration (assessed by error distance) in a spatial navigation task sensitive to grid cells in the entorhinal cortex in 177 volunteers, aged 20-89 years, who did not have self-reported dementia. While place memory was intact even in old age, path integration deteriorated with increasing age. To investigate the relationship between neurofibrillary tangles in the entorhinal cortex and path integration deficit, we examined a mouse model of tauopathy (P301S mutant tau-overexpressing mice; PS19 mice). At 6 months of age, PS19 mice showed a significant accumulation of phosphorylated tau only in the entorhinal cortex, associated with impaired path integration without impairments in spatial cognition. These data are consistent with the idea that path integration deficit is caused by the accumulation of phosphorylated tau in the entorhinal cortex. This method may allow the early identification of individuals likely to develop Alzheimer's disease., Competing Interests: A.K. is employed by MIG Inc. A.T. is a co-founder of MIG Inc. The other authors no competing interests., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Guarantors of Brain.)
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- 2024
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12. Tryptophan-tyrosine dipeptide improves tau-related symptoms in tauopathy mice.
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Ano Y, Takaichi Y, Ohya R, Uchida K, Nakayama H, and Takashima A
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- Mice, Animals, Tryptophan therapeutic use, Dipeptides therapeutic use, Tyrosine, Mice, Transgenic, tau Proteins metabolism, Disease Models, Animal, Alzheimer Disease drug therapy, Alzheimer Disease pathology, Tauopathies drug therapy, Tauopathies prevention & control, Tauopathies metabolism
- Abstract
Neurodegenerative diseases involving pathological tau protein aggregation are collectively known as tauopathies and include Alzheimer's disease and Pick's disease. Recent studies show that the intake of tryptophan-tyrosine (Trp-Tyr)-related β-lactopeptides, including β-lactolin, attenuates cognitive decline in the elderly and prevents the amyloid pathology in mouse models of Alzheimer's disease. However, the effects of Trp-Tyr-related β-lactopeptides on tau-related pathology have not been investigated. In the present study, we examined the effects of Trp-Tyr dipeptide intake on tauopathy in PS19 transgenic mice, a well-established tauopathy model. Intake of Trp-Tyr dipeptide improved the behavioral deficits observed in the open field test, prevented tau phosphorylation, and increased the dopamine turnover and synaptophysin expression in the frontal cortex. Levels of short-chain fatty acids in the cecum were lower in PS19 mice than those in wild-type mice and were increased by treatment with Trp-Tyr dipeptide. In addition, intake of Trp-Tyr dipeptide extended the lifespan of PS19 mice. These findings suggest that the intake of Trp-Tyr-related peptides improves tauopathy symptoms, resulting in improvements in behavioral deficits and longevity. Hence, the intake of Trp-Tyr-related peptides, including β-lactolin, may be beneficial for preventing dementia.
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- 2023
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13. The lysosomal Ragulator complex activates NLRP3 inflammasome in vivo via HDAC6.
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Tsujimoto K, Jo T, Nagira D, Konaka H, Park JH, Yoshimura SI, Ninomiya A, Sugihara F, Hirayama T, Itotagawa E, Matsuzaki Y, Takaichi Y, Aoki W, Saita S, Nakamura S, Ballabio A, Nada S, Okada M, Takamatsu H, and Kumanogoh A
- Subjects
- Mice, Humans, Animals, NLR Family, Pyrin Domain-Containing 3 Protein genetics, Inflammation, Histone Deacetylase 6 genetics, alpha-Tocopherol, Uric Acid, Lysosomes, Mice, Inbred C57BL, Inflammasomes, Peritonitis chemically induced
- Abstract
The cellular activation of the NLRP3 inflammasome is spatiotemporally orchestrated by various organelles, but whether lysosomes contribute to this process remains unclear. Here, we show the vital role of the lysosomal membrane-tethered Ragulator complex in NLRP3 inflammasome activation. Deficiency of Lamtor1, an essential component of the Ragulator complex, abrogated NLRP3 inflammasome activation in murine macrophages and human monocytic cells. Myeloid-specific Lamtor1-deficient mice showed marked attenuation of NLRP3-associated inflammatory disease severity, including LPS-induced sepsis, alum-induced peritonitis, and monosodium urate (MSU)-induced arthritis. Mechanistically, Lamtor1 interacted with both NLRP3 and histone deacetylase 6 (HDAC6). HDAC6 enhances the interaction between Lamtor1 and NLRP3, resulting in NLRP3 inflammasome activation. DL-all-rac-α-tocopherol, a synthetic form of vitamin E, inhibited the Lamtor1-HDAC6 interaction, resulting in diminished NLRP3 inflammasome activation. Further, DL-all-rac-α-tocopherol alleviated acute gouty arthritis and MSU-induced peritonitis. These results provide novel insights into the role of lysosomes in the activation of NLRP3 inflammasomes by the Ragulator complex., (© 2022 The Authors.)
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- 2023
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14. Different Aβ43 deposition patterns in the brains of aged dogs, sea lions, and cats.
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Takahashi K, Chambers JK, Takaichi Y, and Uchida K
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- Animals, Cats, Dogs, Amyloid beta-Peptides metabolism, Peptide Fragments, Plaque, Amyloid veterinary, Plaque, Amyloid pathology, Brain pathology, Sea Lions, Cerebral Amyloid Angiopathy veterinary, Cerebral Amyloid Angiopathy pathology, Alzheimer Disease pathology, Alzheimer Disease veterinary, Cat Diseases, Dog Diseases
- Abstract
Cerebral amyloid β (Aβ) deposition is a pathological hallmark of Alzheimer's disease (AD). There are several molecular species of Aβ, including Aβ40, Aβ42, and Aβ43, and the pathological roles of Aβ43 have attracted particular attention in recent years. Aβ43 is mainly deposited as senile plaques (SPs) in AD brains, and is known to be more amyloidogenic and neurotoxic than Aβ42 and Aβ40. Aβ40 and Aβ42 deposition have been demonstrated in several animal species, while Aβ43 deposition has not been studied in animals. The brains of sea lions, dogs, and cats exhibit unique age-related Aβ pathologies. In the present study, the deposition patterns of Aβ40, Aβ42, and Aβ43 were examined immunohistochemically in the brains of aged dogs (n=52), sea lions (n=5), and cats (n=17). In dogs, most cerebral amyloid angiopathy (CAA) lesions and primitive SPs were positive for Aβ42, Aβ43, and Aβ40. However, diffuse SPs and capillary CAA lesions were negative for Aβ40. In sea lions, all SPs and most CAA lesions were positive for Aβ42, Aβ43, and Aβ40, while capillary CAA lesions were negative for Aβ40. In cats, Aβ42-immunopositive granular aggregates and arteriole and capillary CAA lesions were positive for Aβ43, but negative for Aβ40. Double-labelling immunohistochemistry revealed the co-localization of Aβ42 and Aβ43. These findings suggest that Aβ43 and Aβ42 are frequently deposited in the brains of Carnivora animals and may play an important role in Aβ pathology.
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- 2022
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15. Memory formation in old age requires GSK-3β.
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Koike R, Takaichi Y, Soeda Y, and Takashima A
- Abstract
Glycogen synthase kinase 3β (GSK-3β) is a therapeutic target for various age-related neurodegenerative diseases. It is linked to the two main pathological features of Alzheimer's disease (AD), tau and amyloid β (Aβ); GSK-3β is a major candidate to pathologically hyperphosphorylate tau and modulate Aβ production. However, inhibition of GSK-3β in clinical studies in humans has been found to not significantly improve cognitive function of AD patients, prompting us to study the physiological role of GSK-3β in old mice. Using a contextual fear-conditioning paradigm, we now report that old gsk-3β +/- mice are deficient in both short-term and long-term memory formation, suggesting that GSK-3β is required for memory formation at old age. Biochemical and immunohistochemical analyses showed that the number of synapses does not differ between gsk-3β +/- and age-matched wild-type ( wt ) littermate mice. Based on these observations, we propose that, GSK-3β may contribute to help maintain brain function during aging. Our results may explain the poor efficacy of GSK-3β inhibitors in preserving memory capacity in AD patients., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 The Authors.)
- Published
- 2021
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16. Deposition of Phosphorylated α-Synuclein and Activation of GSK-3β and PP2A in the PS19 Mouse Model of Tauopathy.
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Takaichi Y, Chambers JK, Ano Y, Takashima A, Nakayama H, and Uchida K
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- Animals, Brain metabolism, Brain pathology, Mice, Mutation, Presenilins genetics, Presenilins metabolism, Tauopathies genetics, Tauopathies pathology, alpha-Synuclein genetics, Glycogen Synthase Kinase 3 beta metabolism, Protein Phosphatase 2 metabolism, Tauopathies metabolism, alpha-Synuclein metabolism
- Abstract
The simultaneous accumulation of multiple pathological proteins, such as hyperphosphorylated tau (hp-tau) and phosphorylated α-synuclein (p-αSyn), has been reported in the brains of patients with various neurodegenerative diseases. We previously demonstrated that hp-tau-dependent p-αSyn accumulation was associated with the activation of GSK-3β in the brains of P301L tau transgenic mice. To confirm the effects of another mutant tau on p-αSyn accumulation in vivo, we herein examined the brains of PS19 mice that overexpress human P301S mutant tau. Immunohistochemically, hp-tau and p-αSyn aggregates were detected in the same neuronal cells in the cerebrum and brain stem of aged PS19 mice. A semiquantitative analysis showed a positive correlation between hp-tau and p-αSyn accumulation. Furthermore, an activated form of GSK-3β was detected within cells containing both hp-tau and p-αSyn aggregates in PS19 mice. Western blotting showed a decrease in inactivated PP2A levels in PS19 mice. The present results suggest that the overexpression of human P301S mutant tau induces p-αSyn accumulation that is accompanied by not only GSK-3β, but also PP2A activation in PS19 mice, and highlight the synergic effects between tau and αSyn in the pathophysiology of neurodegenerative diseases that show the codeposition of tau and αSyn., (© 2021 American Association of Neuropathologists, Inc. All rights reserved.)
- Published
- 2021
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17. Feline Spongy Encephalopathy With a Mutation in the ASPA Gene.
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Takaichi Y, Chambers JK, Shiroma-Kohyama M, Haritani M, Une Y, Yamato O, Nakayama H, and Uchida K
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- Amidohydrolases genetics, Amidohydrolases metabolism, Animals, Brain diagnostic imaging, Brain metabolism, Cats, Mutation, Canavan Disease veterinary, Cat Diseases genetics, Neurodegenerative Diseases veterinary
- Abstract
Canavan disease is an autosomal recessive leukodystrophy caused by mutations in the gene encoding aspartoacylase (ASPA), which hydrolyses N-acetylaspartate (NAA) to acetate and aspartate. A similar feline neurodegenerative disease associated with a mutation in the ASPA gene is reported herein. Comprehensive clinical, genetic, and pathological analyses were performed on 4 affected cats. Gait disturbance and head tremors initially appeared at 1 to 19 months of age. These cats eventually exhibited dysstasia and seizures and died at 7 to 53 months of age. Magnetic resonance imaging of the brain revealed diffuse symmetrical intensity change of the cerebral cortex, brainstem, and cerebellum. Gas chromatography-mass spectrometry analysis of urine showed significant excretion of NAA. Genetic analysis of the 4 affected cats identified a missense mutation (c.859G>C) in exon 6 of the ASPA gene, which was not detected in 4 neurologically intact cats examined as controls. Postmortem analysis revealed vacuolar changes predominantly distributed in the gray matter of the cerebrum and brain stem as well as in the cerebellar Purkinje cell layer. Immunohistochemically, these vacuoles were surrounded by neurofilaments and sometimes contained MBP- and Olig2-positive cells. Ultrastructurally, a large number of intracytoplasmic vacuoles containing mitochondria and electron-dense granules were detected in the cerebral cortex. All 4 cats were diagnosed as spongy encephalopathy with a mutation in the ASPA gene, a syndrome analogous to human Canavan disease. The histopathological findings suggest that feline ASPA deficiency induces intracytoplasmic edema in neurons and oligodendrocytes, resulting in spongy degeneration of the central nervous system.
- Published
- 2021
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18. Amyloid β and tau pathology in brains of aged pinniped species (sea lion, seal, and walrus).
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Takaichi Y, Chambers JK, Takahashi K, Soeda Y, Koike R, Katsumata E, Kita C, Matsuda F, Haritani M, Takashima A, Nakayama H, and Uchida K
- Subjects
- Aging pathology, Animals, Brain pathology, Caniformia, Female, Male, Phoca, Sea Lions, Walruses, Aging metabolism, Amyloid beta-Peptides metabolism, Brain metabolism, tau Proteins metabolism
- Abstract
Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β (Aβ) as senile plaques and cerebral amyloid angiopathy, and hyperphosphorylated tau (hp-tau) as neurofibrillary tangles in the brain. The AD-related pathology has been reported in several non-human animals, and most animals develop only the Aβ or tau pathology. We herein describe the Aβ and hp-tau pathology in the brains of aged pinniped species (seal, sea lion, and walrus). Molecular analyses revealed that the sequence of pinniped Aβ was identical to that of human Aβ. Histopathological examinations detected argyrophilic plaques composed of Aβ associated with dystrophic neurites in the cerebral cortex of aged pinnipeds. Astrogliosis and microglial infiltration were identified around Aβ plaques. Aβ deposits were observed in the blood vessel walls of the meninges and cerebrum. Pinniped tau protein was physiologically subjected to alternative splicing at exons 2, 3, and 10, and presented as five isoforms: two 3-repeat tau isoforms (1N3R, 2N3R) and three 4-repeat tau isoforms (0N4R, 1N4R, 2N4R); 0N3R tau isoform was absent. Histopathological examinations revealed argyrophilic fibrillar aggregates composed of hp-tau in the neuronal somata and neurites of aged pinniped brains. Few hp-tau aggregates were found in oligodendrocytes and microglia. Biochemically, hp-tau of the 3-repeat and 4-repeat isoforms was detected in brain sarkosyl-insoluble fractions. Aβ and hp-tau both predominantly accumulated in the neocortex, particularly the frontal cortex. Furthermore, the activation of GSK-3β was detected within cells containing hp-tau aggregates, and activated GSK-3β was strongly expressed in cases with severe hp-tau pathologies. The present results suggest that, in association with Aβ deposition, the activation of GSK-3β contributes to hp-tau accumulation in pinniped brains. Here, we report that pinniped species naturally accumulate Aβ and tau with aging, similar to the human AD pathology.
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- 2021
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19. Feline Niemann-Pick Disease With a Novel Mutation of SMPD1 Gene.
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Takaichi Y, Chambers JK, Kok MK, Uchiyama H, Haritani M, Hasegawa D, Nakayama H, and Uchida K
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- Animals, Autopsy veterinary, Brain pathology, Brain ultrastructure, Cats, Female, Histocytochemistry veterinary, Macrophages pathology, Microscopy, Electron, Transmission veterinary, Mutation, Nervous System pathology, Neurons pathology, Niemann-Pick Diseases genetics, Niemann-Pick Diseases pathology, Cat Diseases pathology, Niemann-Pick Diseases veterinary, Sphingomyelin Phosphodiesterase genetics
- Abstract
A 4-month-old female mixed-breed cat showed gait disturbance and eventual dysstasia with intention tremor and died at 14 months of age. Postmortem histological analysis revealed degeneration of neuronal cells, alveolar epithelial cells, hepatocytes, and renal tubular epithelial cells. Infiltration of macrophages was observed in the nervous system and visceral organs. The cytoplasm of neuronal cells was filled with Luxol fast blue (LFB)-negative and periodic acid-Schiff (PAS)-negative granules, and the cytoplasm of macrophages was LFB-positive and PAS-negative. Ultrastructurally, concentric deposits were observed in the brain and visceral organs. Genetic and biochemical analysis revealed a nonsense mutation (c.1017G>A) in the SMPD1 gene, a decrease of SMPD1 mRNA expression, and reduced acid sphingomyelinase immunoreactivity. Therefore, this cat was diagnosed as having Niemann-Pick disease with a mutation in the SMPD1 gene, a syndrome analogous to human Niemann-Pick disease type A.
- Published
- 2020
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20. Phosphorylation and oligomerization of α-synuclein associated with GSK-3β activation in the rTg4510 mouse model of tauopathy.
- Author
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Takaichi Y, Chambers JK, Inoue H, Ano Y, Takashima A, Nakayama H, and Uchida K
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- Animals, Brain metabolism, Disease Models, Animal, Humans, Mice, Inbred C57BL, Mice, Transgenic, Phosphorylation, Brain pathology, Glycogen Synthase Kinase 3 beta metabolism, Protein Aggregation, Pathological metabolism, Tauopathies metabolism, Tauopathies pathology, alpha-Synuclein metabolism, tau Proteins metabolism
- Abstract
Neurodegenerative diseases are characterized by the accumulation of specific phosphorylated protein aggregates in the brain, such as hyperphosphorylated tau (hp-tau) in tauopathies and phosphorylated α-synuclein (p-αSyn) in α-synucleinopathies. The simultaneous accumulation of different proteins is a common event in many neurodegenerative diseases. We herein describe the detection of the phosphorylation and dimerization of αSyn and activation of GSK-3β, a major kinase known to phosphorylate tau and αSyn, in the brains of rTg4510 mice that overexpress human P301L mutant tau. Immunohistochemistry showed p-αSyn aggregates in rTg4510 mice, which were suppressed by doxycycline-mediated decreases in mutant tau expression levels. A semi-quantitative analysis revealed a regional correlation between hp-tau and p-αSyn accumulation in rTg4510 mice. Furthermore, proteinase K-resistant αSyn aggregates were found in the region with excessive hp-tau accumulation in rTg4510 mice, and these aggregates were morphologically different from proteinase K-susceptible p-αSyn aggregates. Western blotting revealed decreases in p-αSyn monomers in TBS- and sarkosyl-soluble fractions and increases in ubiquitinated p-αSyn dimers in sarkosyl-soluble and insoluble fractions in rTg4510 mice. Furthermore, an activated form of GSK-3β was immunohistochemically detected within cells containing both hp-tau and p-αSyn aggregates. A semi-quantitative analysis revealed that increased GSK-3β activity strongly correlated with hp-tau and p-αSyn accumulation in rTg4510 mice. Collectively, the present results suggest that the overexpression of human P301L mutant tau promoted the phosphorylation and dimerization of endogenous αSyn by activating GSK-3β in rTg4510 mice. This synergic effect between tau, αSyn, and GSK-3β may be involved in the pathophysiology of several neurodegenerative diseases that show the accumulation of both tau and αSyn.
- Published
- 2020
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21. β-Lactolin, a Whey-Derived Lacto-Tetrapeptide, Prevents Alzheimer's Disease Pathologies and Cognitive Decline.
- Author
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Ano Y, Ohya R, Takaichi Y, Washinuma T, Uchida K, Takashima A, and Nakayama H
- Subjects
- Alzheimer Disease pathology, Alzheimer Disease psychology, Amyloid beta-Peptides metabolism, Animals, Behavior, Animal drug effects, Cognitive Dysfunction pathology, Cognitive Dysfunction psychology, Cytokines metabolism, Dopamine metabolism, Macrophage Activation drug effects, Memory drug effects, Mice, Mice, Transgenic, Microglia drug effects, Neurotransmitter Agents metabolism, Psychomotor Performance drug effects, Tauopathies drug therapy, Alzheimer Disease prevention & control, Cognitive Dysfunction prevention & control, Oligopeptides therapeutic use, Whey Proteins therapeutic use
- Abstract
The prevention of age-related memory decline and dementia has been becoming a high priority because of the rapid growth in aging populations. Accumulating epidemiological and clinical studies indicate that intake of fermented dairy products rich in β-lactolin improves memory retrieval and executive function and attenuates cognitive decline in the elderly. However, the effects of long-term consumption of β-lactolin on Alzheimer's disease (AD) pathologies have not been investigated. In the present study, we examined the effects of β-lactolin and whey digestion rich in β-lactolin on AD pathology in 5×FAD transgenic mice and PS19 tauopathy mice. Intake of β-lactolin and whey digestion rich in β-lactolin reduced the levels of inflammatory cytokines, suppressed the infiltration of activated microglia, decreased the levels of amyloid-β, ameliorated impaired long-term object memory, and attenuated decreased synaptophysin, dopamine, brain-derived neurotrophic factor, and insulin-like growth factor 1 levels in the cortex in 5×FAD transgenic mice. In addition, intake of β-lactolin and whey digestion rich in β-lactolin improved behavioral abnormality and reduced the ratio of phosphorylated tau to total tau in the cortex in PS19 tauopathy mice. These findings indicate that consumption with β-lactolin and whey digestion rich in β-lactolin suppresses inflammation and attenuates AD pathology and cognitive impairment.
- Published
- 2020
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22. Iso-α-Acids, Bitter Components in Beer, Suppress Inflammatory Responses and Attenuate Neural Hyperactivation in the Hippocampus.
- Author
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Ano Y, Yoshikawa M, Takaichi Y, Michikawa M, Uchida K, Nakayama H, and Takashima A
- Abstract
Due to the growth in aging populations worldwide, prevention and therapy for age-related cognitive decline and dementia are in great demand. We previously demonstrated that long-term intake of iso-α-acids, which are hop-derived bitter compounds found in beer, prevent Alzheimer's pathology in a rodent model. On the other hand, the effects of iso-α-acids on neural activity in Alzheimer's disease model mice have not been investigated. Here, we demonstrated that short-term intake of iso-α-acids suppresses inflammation in the hippocampus and improves memory impairment even after disease onset. Importantly, we demonstrated that short-term administration of iso-α-acids attenuated the neural hyperactivation in hippocampus. In 6-month-old 5 × FAD mice exhibiting hippocampus inflammation and memory impairment, oral administration of iso-α-acids for 7 days reduced inflammatory cytokines, including MIP-1α and soluble Aβ and improved object memory in the novel object recognition test. In 12-month-old J20 mice, intake of iso-α-acids for 7 days also suppressed inflammatory cytokines and soluble Aβ in the brain. Manganese-enhanced magnetic resonance imaging (MEMRI) of hippocampi of J20 mice showed increased manganese compared with wild type mice, but iso-α-acids canceled this increased MEMRI signal in J20 mice, particularly in the hippocampus CA1 and CA3 region. Taken together, these findings suggest that short-term intake of iso-α-acids can suppress hippocampus inflammation even after disease onset and improve hyper neural activity in Alzheimer's disease model mice.
- Published
- 2019
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23. Novel lactopeptides in fermented dairy products improve memory function and cognitive decline.
- Author
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Ano Y, Ayabe T, Kutsukake T, Ohya R, Takaichi Y, Uchida S, Yamada K, Uchida K, Takashima A, and Nakayama H
- Subjects
- Animals, Disease Models, Animal, Dopamine metabolism, Dopamine Antagonists pharmacology, Male, Mice, Mice, Inbred C57BL, Rats, Rats, Sprague-Dawley, Aging, Amnesia diet therapy, Cognitive Dysfunction diet therapy, Cultured Milk Products, Dipeptides pharmacology, Dopamine analogs & derivatives, Monoamine Oxidase metabolism, Whey Proteins
- Abstract
Alongside the rapid growth in aging populations, prevention of age-related memory decline and dementia has become a high priority. Several epidemiological and clinical studies have concluded that fermented dairy products can help to prevent cognitive decline; furthermore, intake of Camembert cheese prevents Alzheimer's pathology in model mice. To elucidate molecular mechanisms underlying the preventive effects of fermented dairy products, here we screened peptides from digested fermented dairy products for ability to improve memory function in a scopolamine-induced amnesia mouse model. We found that Trp-Tyr (WY)-containing peptides from whey protein improved memory function in the mice, and the effects were confirmed in aged mice. The WY-containing peptides directly inhibited monoamine oxidase-B activity and increased dopamine levels in brain tissue. Pretreatment with dopamine receptor antagonist abolished the improvement in memory function due to WY-containing peptides. These results suggest that WY-containing peptides in fermented dairy products increase monoamine levels by inhibiting monoamine oxidase-B activity, helping to prevent age-related cognitive decline., (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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24. Iso-α-Acids, the Bitter Components of Beer, Suppress Microglial Inflammation in rTg4510 Tauopathy.
- Author
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Ano Y, Takaichi Y, Uchida K, Kondo K, Nakayama H, and Takashima A
- Subjects
- Acids chemistry, Acids pharmacology, Animals, Brain pathology, Mice, Inbred C57BL, Mice, Transgenic, Microglia drug effects, Phenotype, Phosphorylation drug effects, Acids therapeutic use, Beer analysis, Inflammation pathology, Microglia pathology, Taste, Tauopathies pathology
- Abstract
Due to the growth in aging populations, prevention for cognitive decline and dementia are in great demand. We previously demonstrated that the consumption of iso-α-acids (IAA), the hop-derived bitter compounds in beer, prevents inflammation and Alzheimer's disease pathology in model mice. However, the effects of iso-α-acids on inflammation induced by other agents aside from amyloid β have not been investigated. In this study, we demonstrated that the consumption of iso-α-acids suppressed microglial inflammation in the frontal cortex of rTg4510 tauopathy mice. In addition, the levels of inflammatory cytokines and chemokines, including IL-1β and MIP-1β, in the frontal cortex of rTg4510 mice were greater than those of wild-type mice, and were reduced in rTg4510 mice fed with iso-α-acids. Flow cytometry analysis demonstrated that the expression of cells producing CD86, CD68, TSPO, MIP-1α, TNF-α, and IL-1β in microglia was increased in rTg4510 mice compared with wild-type mice. Furthermore, the expression of CD86- and MIP-1α-producing cells was reduced in rTg4510 mice administered with iso-α-acids. Moreover, the consumption of iso-α-acids reduced the levels of phosphorylated tau in the frontal cortex. Collectively, these results suggest that the consumption of iso-α-acids prevents the inflammation induced in tauopathy mice. Thus, iso-α-acids may help in preventing inflammation-related brain disorders.
- Published
- 2018
- Full Text
- View/download PDF
25. Deposition of Phosphorylated α-Synuclein in the rTg4510 Mouse Model of Tauopathy.
- Author
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Takaichi Y, Ano Y, Chambers JK, Uchida K, Takashima A, and Nakayama H
- Subjects
- Animals, Humans, Mice, Mice, Inbred C57BL, Mice, Transgenic, Phosphorylation physiology, Protein Aggregation, Pathological pathology, Tauopathies pathology, Disease Models, Animal, Protein Aggregation, Pathological metabolism, Tauopathies metabolism, alpha-Synuclein metabolism, tau Proteins metabolism
- Abstract
The accumulation of specific phosphorylated protein aggregates in the brain is a hallmark of severe neurodegenerative disorders. Specifically, hyperphosphorylated tau (hp-tau) accumulates in Alzheimer disease, frontotemporal dementia with Parkinsonism linked to chromosome 17, and progressive supranuclear palsy; furthermore, phosphorylated α-synuclein (p-αSyn) accumulates in Parkinson disease, dementia with Lewy bodies, and multiple system atrophy. Moreover, codeposition of different pathological protein aggregates is common in the brains of individuals with neurodegenerative diseases. In the present report, we describe the detection of p-αSyn aggregates in the brain of rTg4510 mice that overexpress human P301L mutant tau. Immunohistochemistry showed that hp-tau and p-αSyn aggregates were found within the same neuronal cells in rTg4510 mice and increased with age. Moreover, semiquantitative analysis revealed a significant regional correlation between hp-tau and p-αSyn accumulation. These results indicate that endogenous mouse αSyn protein is phosphorylated and accumulates with hp-tau aggregation in neurons and suggest that the overexpression of human P301L mutant tau may enhance endogenous αSyn phosphorylation and aggregation via a similar hyperphosphorylation mechanism in vivo. This synergic effect between tau and αSyn accumulation may exacerbate the pathology of several neurodegenerative disorders that show a cooccurrence of hp-tau and p-αSyn aggregation.
- Published
- 2018
- Full Text
- View/download PDF
26. Therapeutic effect of novel anti-human Fas antibody HFE7a on graft-versus-host disease model.
- Author
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Kuwahara H, Tani Y, Ogawa Y, Takaichi Y, Shiraishi A, and Ohtsuki M
- Subjects
- Acute Disease, Animals, Apoptosis, Chronic Disease, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, SCID, Mice, Transgenic, Antibodies, Monoclonal therapeutic use, Graft vs Host Disease therapy, fas Receptor physiology
- Abstract
In order to evaluate anti-human Fas antibody, we have established a new graft-versus-host disease (GVHD) model wherein splenocytes of human Fas transgenic mice (hFas-TgM) were transferred to immune-deficient SCID mice. In this model, although host SCID cells are not activated by or responsive to graft hFas-TgM cells, graft hFas-TgM cells are activated by and responsive to host SCID cells and thus cause GVHD symptoms. SCID mice that received hFas-TgM splenocytes had increased human Fas-positive lymphocytes in lymph nodes, decreased in body weight, and developed skin diseases, including rash and alopecia. Administration of novel anti-human Fas antibody HFE7A, which did not induce liver toxicity after administration to mice, decreased the level of the human Fas-positive lymphocytes, blocked the decrease of body weight, and suppressed development of skin diseases in this model. These results indicate that induction of apoptosis to activated graft cells with nontoxic anti-Fas antibody could reduce GVHD symptoms., (Copyright 2001 Academic Press.)
- Published
- 2001
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27. Calorimetric investigations of phase transitions of sonicated vesicles of dimyristoylphosphatidylcholine.
- Author
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Kodama M, Miyata T, and Takaichi Y
- Subjects
- Calorimetry, Differential Scanning, Gels, Liposomes chemistry, Microscopy, Electron, Sonication, Temperature, Thermodynamics, Dimyristoylphosphatidylcholine chemistry
- Abstract
The process of conversion of large multilamellar vesicles (MLVs) of dimyristoylphosphatidylcholine (DMPC) into the final state of small unilamellar vesicles (SUVs) with an increase in time length of ultrasonic irradiation was investigated by calorimetry and negative-stain electron microscopy. The process was found out to be composed of two stages depending on the primary (near 24 degrees C) and secondary (near 19 degrees C) peaks due to the gel-to-liquid crystal phase (Tm) transition, respectively; a new transition peak for the secondary Tm appears after a maximum broadening of the primary Tm peak is attained. Sonicated vesicles characterized by the primary peak of the broadest shape were observed to be about 200 nm in mean diameter and mostly four or so lamellae, and have an internal aqueous space, in contrast to sonicated SUVs (approx. 40 nm in diameter) characterized by the limiting secondary Tm peak. Thermal data associated with the Tm transition for these two sonicated vesicles were compared with that of the MLV. The enthalpy and entropy changes and cooperative units increased in the order sonicated SUV < sonicated large vesicle < MLV. Furthermore, the enthalpy changes were revealed to fairly differ between the sonicated large vesicle and SUV. Based on the effect of the annealing treatment at -5 degrees C on these vesicles the present result suggested a large contribution of the aggregation state of DMPC molecules to the enthalpy possessed by the vesicles of a gel phase temperature, which is related to the mode of the Tm transitions, primary and secondary.
- Published
- 1993
28. Development of spontaneous hypothyroidism in patients with Graves' disease treated with antithyroidal drugs: clinical, immunological, and histological findings in 26 patients.
- Author
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Tamai H, Kasagi K, Takaichi Y, Takamatsu J, Komaki G, Matsubayashi S, Konishi J, Kuma K, Kumagai LF, and Nagataki S
- Subjects
- Adolescent, Adult, Female, Follow-Up Studies, Graves Disease immunology, Humans, Hypothyroidism immunology, Hypothyroidism pathology, Immunoglobulins, Thyroid-Stimulating, Male, Middle Aged, Thyroid Function Tests, Thyroid Gland immunology, Thyroid Gland pathology, Thyroiditis, Autoimmune immunology, Antibodies analysis, Antithyroid Agents adverse effects, Graves Disease drug therapy, Hypothyroidism chemically induced, Immunoglobulin G analysis
- Abstract
Graves' disease may result eventually in hypothyroidism in approximately 5-20% of patients. In a few such patients hypothyroidism was associated with TSH-blocking antibodies, but whether the frequency of TSH-blocking antibodies in such patients is as high as it is (21%) in patients with primary myxedema is not known. This study was undertaken to determine the presence of various immunoglobulins [TSH binding inhibitor immunoglobulins, thyroid-stimulating antibodies (TSAb), and TSH-blocking antibodies] in 26 patients with Graves' disease who developed hypothyroidism from 0.5-10 yr or more after discontinuation of antithyroid drug therapy. Eight of the 26 patients (31%) had TSH-blocking antibodies, 16 (61%) had TSAb, and 14 (54%) had thyroid hormone binding inhibitor immunoglobulins. Thyroid needle biopsies were performed in 9 patients. Three of 5 patients who had subclinical hypothyroidism had chronic lymphocytic thyroiditis, and all had positive TSAb titers. Three patients had the fibrous variant of chronic lymphocytic thyroiditis; their TSAb values were 902%, 431%, and 1290%. One patient had follicular hyperplasia. We conclude that TSH-blocking antibodies may account for hypothyroidism in approximately one third of patients with Graves' disease who were previously treated with antithyroid drugs, and that autoimmune thyroiditis is comparable for the hypothyroidism in the remaining two thirds of Graves' disease patients.
- Published
- 1989
- Full Text
- View/download PDF
29. The dexamethasone suppression test for Japanese with eating disorders.
- Author
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Fukata S, Tamai H, Takaichi Y, Mori K, Matsubayashi S, and Nakagawa T
- Subjects
- Anorexia Nervosa psychology, Bulimia psychology, Female, Humans, Patient Compliance, Anorexia Nervosa blood, Bulimia blood, Dexamethasone pharmacokinetics, Hydrocortisone blood
- Abstract
A one-mg oral overnight dexamethasone suppression test (DST) was conducted on 22 inpatients with eating disorders. To confirm that the dexamethasone tablets had been ingested, we measured the plasma concentrations of dexamethasone the next morning (at 0900 hr after DST). The diagnosis of anorexia nervosa and bulimia was made according to the criteria for DSM-III, respectively. Of the 22 patients with eating disorders, 16 satisfied the criteria for anorexia nervosa and 6 for bulimia. The DST was carried out within 2 weeks of hospitalization on each patient. The subjects were given 1 mg of dexamethasone in the evening (at 2300 hr) and blood samples were collected the following day (at 0900, 1600 and 2100 hr, respectively). The plasma cortisol and dexamethasone levels were concurrently determined by RIA. The criterion for non-suppression was a failure to suppress the plasma cortisol levels below 5.0 micrograms/dl in any one of the three samples. All but one patient with bulimia had ingested the dexamethasone. Thirteen (62%) of 21 patients with eating disorders were nonsuppressors. We found a significant positive correlation between the plasma cortisol levels at 1600 hr or 2100 hr and a decrease in ideal body weight (n = 16, r = 0.613, p less than 0.05; r = 0.75, p less than 0.01, respectively) and a significant inverse relationship between the plasma dexamethasone levels at 0900 hr and the plasma cortisol levels at 1600 hr was recognized (n = 21, r = 0.631, p less than 0.01). These results suggest that the blood dexamethasone levels as well as body weight might contribute to the abnormalities of DST seen in patients with eating disorders.
- Published
- 1988
- Full Text
- View/download PDF
30. Impaired glucagon secretion to insulin-induced hypoglycemia in anorexia nervosa.
- Author
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Fujii S, Tamai H, Kumai M, Takaichi Y, Nakagawa T, and Aoki TT
- Subjects
- Adolescent, Adult, Anorexia Nervosa blood, Anorexia Nervosa diet therapy, Blood Glucose analysis, Female, Glucagon blood, Humans, Hypoglycemia, Insulin blood, Anorexia Nervosa physiopathology, Glucagon metabolism, Insulin administration & dosage, Islets of Langerhans physiopathology
- Abstract
In order to clarify the role played by pancreatic alpha-cell dysfunction in the impaired glucose recovery from hypoglycemia in patients with anorexia nervosa, the response of pancreatic alpha-cells to insulin-induced hypoglycemia was investigated in 16 patients with anorexia nervosa before and after treatment. The results were compared with those obtained after loading with arginine. Before treatment, despite comparable falls in plasma glucose levels, glucagon secretion was significantly reduced in the anorectic patients compared with control subjects. In addition, glucose recovery from hypoglycemia in the patients was attenuated. However, after treatment, both glucagon secretory activity and plasma glucose recovery following insulin-induced hypoglycemia were restored to normal. Plasma glucagon responses to arginine infusion were not significantly different in the untreated anorectic patients and control subjects. However, the plasma insulin response in the patients was significantly lower than in the control group. These results suggest that the impaired recovery of plasma glucose levels from insulin-induced hypoglycemia in patients with anorexia nervosa is primarily attributable to impaired pancreatic alpha-secretory capability. In addition, this abnormality in pancreatic alpha-cell function is reversible with treatment leading to improved nutrition and weight gain.
- Published
- 1989
- Full Text
- View/download PDF
31. The significance of antithyroglobulin and antithyroidal microsomal antibodies in patients with hyperthyroidism due to Graves' disease treated with antithyroidal drugs.
- Author
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Takaichi Y, Tamai H, Honda K, Nagai K, Kuma K, and Nakagawa T
- Subjects
- Adolescent, Adult, Female, Graves Disease complications, Humans, Hyperthyroidism drug therapy, Hyperthyroidism etiology, Immune Sera immunology, Male, Middle Aged, Thyroid Function Tests, Thyroid Gland immunology, Thyroid Gland pathology, Antibodies immunology, Antithyroid Agents therapeutic use, Graves Disease immunology, Hyperthyroidism immunology, Methimazole therapeutic use, Microsomes immunology, Thyroglobulin immunology
- Abstract
The presence of serum antithyroglobulin (TGHA) and antithyroidal microsomal (MCHA) antibodies in Graves' disease patients is associated with lymphocytic infiltration of the thyroid. The aim of this study was to determine the clinical significance of TGHA and MCHA during and after treatment of hyperthyroidism due to Graves' disease. One hundred and seventeen such patients were treated for 2 yr with methimazole and then followed for an additional year or more (mean, 30 months). The patients were classified into the following three groups: group I, patients negative for TGHA and MCHA before and during the 2 yr of treatment; group II, patients positive for MCHA but negative for TGHA before and during the 2 yr of treatment; and group III, patients who were positive for both TGHA and MCHA before and during treatment. The relapse rates after discontinuation of treatment in these groups were 39% (13 of 33), 27% (13 of 48), and 11% (4 of 36), respectively; the value in group I was significantly higher than that in group III (P less than 0.01). The results suggest that the presence of TGHA and MCHA may influence the prognosis of Graves' disease in patients treated with methimazole. Those patients who had neither antibody before and during treatment were most likely to have a relapse of hyperthyroidism, and those who had both antibodies were least likely to have a relapse.
- Published
- 1989
- Full Text
- View/download PDF
32. Methimazole-induced agranulocytosis in Japanese patients with Graves' disease.
- Author
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Tamai H, Takaichi Y, Morita T, Komaki G, Matsubayashi S, Kuma K, Walter RM Jr, Kumagai LF, and Nagataki S
- Subjects
- Adult, Agranulocytosis ethnology, Female, Humans, Japan, Leukocyte Count drug effects, Male, Middle Aged, Thyroid Function Tests, Agranulocytosis chemically induced, Graves Disease drug therapy, Methimazole adverse effects
- Abstract
We reviewed the records of approximately 7000 Japanese patients whose hyperthyroidism was treated with methimazole (MMI) alone. Four patients (Group I) developed agranulocytosis during a second course of MMI therapy and eight patients (Group II) during an initial course. Six patients (three in each group) received less than 30 mg MMI daily. Agranulocytosis occurred after more than 2 months of therapy (12 weeks-1 year) in five patients. Seven patients were less than 40 years of age. One patient displayed a gradual protracted development of agranulocytosis. These results indicate that agranulocytosis after MMI may occur irrespective of dose, age, duration of treatment, and with a second exposure.
- Published
- 1989
- Full Text
- View/download PDF
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