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Additional file 1: Fig. S1. The expression pattern of SRSF1 in ovarian development and ageing. a The expression of Srsf1 during ovarian development and ageing. Real-time qPCR data were normalized to Gapdh. b Western blotting of SRSF1 expression in 17.5 dpc Ctrl and cKO ovaries. c The relative protein expression level of SRSF1 is shown in ovarian development and ageing. ACTB served as a loading control. The value in 12 M ovaries was set as 1.0. d Immunostaining was performed using VASA and SRSF1 antibodies from NB, 6 W, and 10 M ovaries. DNA was stained with DAPI. Scale bar, 10 μm. NB, newborn; W, week; M, month.

Additional file 3: Table 2. AS events were analysed in cKO and Ctrl ovaries.

Additional file 5: Fig. S3. Various genes were visually analysed using IGV.

Additional file 6: Table 3. Primer sequences were used in this study.

Additional file 8. Uncropped gels/blots.

Additional file 2.

Additional file 1.

Additional file 4: Fig. S2. SRSF1 regulates the stability of Msh5 and Six6os1. a, b Western blotting of MSH5 and SIX6OS1 expression in 17.5 dpc Ctrl and cKO ovaries. GAPDH (a) or ACTB (b) served as a loading control. c The expression of Msh5 in 16.5 dpc cKO ovaries after ActD treatment at different times. n=4.

Additional file 7: Table 4. Antibodies were used in this study.

Additional file 2: Table 1. Differential genes were analysed in this study.

The coexistence of human beings and environmental sustainability becomes individually and even globally concerned issue. In addition to environment issues, people also encounter negative issues of infectious diseases, gap between rich and poor, violence in society, uneven resource distribution, people’s health decline, and population structure aging, which would affect the sustainable development of cities. When taking sustainable development from the world to cities, it appears sustainable cities. The goal of a healthy city is to pursue the sustainability of a city. Aiming at residents in Shanghai, total 360 copies of questionnaire are distributed, and 277 valid copies are retrieved, with the retrieval rate 77％. The research results conclude that 1.“safety” is the most emphasized dimension, followed by “convenience & prosperity”, “sustainable ecology”, “vitality & health”, and “culture friendly” and 2.security, Internet city, pollution control, space use, and inheritance education are top five indicators, among 15 evaluation indicators. According to the results, suggestions are proposed to provide the government with correct, objective, and simply understandable healthy city indicators and sustainable development indicators for the appropriate planning and review of the administration objectives to enhance the public awareness of healthy city and the participation. It would stop the worsening of environment and promote residents’ health and the sustainable development of cities to have the city and the public moving towards healthy development.

Digital technology and Internet have been combined with modern people’s life, and the combination of bit technology with network communication systems largely change people’s lifestyles. Along with the popularity of education, the application of digital technology also enhances the basic changes of learning methods and learning contents. To provide quality service, professional librarians are necessary for a library. Accordingly, librarians are the core management element of a library. In the changeable technology era, librarians need constant learning for self-growth through continuing education in order to cope with the changeable environment. Taking librarians of Shanghai University of Medicine & Health Sciences as the research object, the librarians are proceeded continuing education with digital technology, and the questionnaire is distributed and collected on-site after the continuing education. The research results show that 1.continuing education would significantly and positively affect professional growth, 2.professional growth would remarkably and positively affect job satisfaction, and 3.continuing education would notably and positively affect job satisfaction. With such results, suggestions are proposed, expecting to apply the professional curricula of continuing education with digital technology for the continuous growth of librarians in the changeable era, satisfying the enhancement of core competencies to cope with reader needs under the time and technology changes, and further promoting domestic librarians’ professional competence and the development of library business.