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812 results on '"Stolk, J"'

1. Cigarette smoke restricts the ability of mesenchymal cells to support lung epithelial organoid formation.

Author

Khedoe, P. P. S. J., van Schadewijk, W. A. A. M., Schwiening, M., Ng-Blichfeldt, J. P., Marciniak, S. J., Stolk, J., Gosens, R., and Hiemstra, P. S.

Subjects
UNFOLDED protein response, CHRONIC obstructive pulmonary disease, CIGARETTE smoke, SMOKING, FIBROBLASTS, WNT genes
Abstract

Adequate lung epithelial repair relies on supportive interactions within the epithelial niche, including interactions with WNT-responsive fibroblasts. In fibroblasts from patients with chronic obstructive pulmonary disease (COPD) or upon in vitro cigarette smoke exposure, Wnt/ß-catenin signalling is distorted, which may affect interactions between epithelial cells and fibroblasts resulting in inadequate lung repair. We hypothesized that cigarette smoke (CS), the main risk factor for COPD, interferes with Wnt/ß-catenin signalling in fibroblasts through induction of cellular stress responses, including oxidative- and endoplasmic reticulum (ER) stress, and thereby alters epithelial repair support potential. Therefore, we assessed the effect of CS-exposure and the ER stress inducer Thapsigargin (Tg) on Wnt/ß-catenin signalling activation in MRC-5 fibroblasts, and on their ability to support lung epithelial organoid formation. Exposure of MRC-5 cells for 15 min with 5 AU/mL CS extract (CSE), and subsequent 6 h incubation induced oxidative stress (HMOX1). Whereas stimulation with 100 nM Tg increased markers of both the integrated stress response (ISR - GADD34/PPP1R15A, CHOP) and the unfolded protein response (UPR - XBP1spl, GADD34/PPP1R15A, CHOP and HSPA5/BIP), CSE only induced GADD34/PPP1R15A expression. Strikingly, although treatment of MRC-5 cells with the Wnt activator CHIR99021 upregulated the Wnt/ß-catenin target gene AXIN2, this response was diminished upon CSE or Tg pre-exposure, which was confirmed using a Wnt-reporter. Furthermore, pre-exposure of MRC-5 cells to CSE or Tg, restricted their ability to support organoid formation upon co-culture with murine pulmonary EpCam+ cells in Matrigel at day 14. This restriction was alleviated by pre-treatment with CHIR99021. We conclude that exposure of MRC-5 cells to CSE increases oxidative stress, GADD34/PPP1R15A expression and impairs their ability to support organoid formation. This inhibitory effect may be restored by activating the Wnt/ß-catenin signalling pathway. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Patterns and characterization of COPD exacerbations using real-time data collection

Author

Ejiofor SI, Stolk J, Fernandez P, and Stockley RA

Subjects
Exacerbations, Alpha-1 antitrypsin deficiency, COPD, Diseases of the respiratory system, RC705-779
Abstract

Stanley I Ejiofor,1,2 Jan Stolk,3 Pablo Fernandez,4 Robert A Stockley1,2 1Centre for Translational Inflammation Research, University of Birmingham, 2ADAPT Project, University Hospital Birmingham, Birmingham, UK; 3Leiden University Medical Centre, Leiden, the Netherlands; 4Independent consultant, Penn, UK Introduction: Patients with chronic obstructive pulmonary disease often experience exacerbations. These events are important as they are a major cause of morbidity and mortality. Recently, it has been increasingly recognized that patients may experience symptoms suggestive of an exacerbation but do not seek treatment, which are referred to as unreported or untreated exacerbations. Symptom diaries used in clinical trials have the benefit of identifying both treated and untreated exacerbation events. Methods: The Kamada study was a multicenter, double-blind randomized controlled trial of inhaled augmentation therapy in alpha-1 antitrypsin deficiency (AATD). A retrospective review of daily electronic symptom diary cards was undertaken from the two leading centers to identify symptomatic episodes consistent with a definition of an exacerbation. The aims were to explore the relationship between exacerbation events and classical “Anthonisen” symptoms and to characterize treated and untreated episodes. Results: Forty-six AATD patients with airflow obstruction and history of exacerbations were included in the analysis. Two hundred thirty-three exacerbation episodes were identified: 103 untreated and 130 treated. Untreated episodes were significantly shorter (median 6 days; interquartile range [IQR] 3–10 days) than the treated episodes (median 10 days; IQR 5–18.25 days: P

Published
2017

11. Abstracts of poster presentations

Author

Agostinho, A. B., Rosi, F., Tabucchi, A., Carlucci, F., Pizzichini, M., Arnér, Elias S. J., Eriksson, Staffan, Barankiewicz, J., Trembacz, H., Zwierzchowski, L., Bergman, A. M., van Haperen, V. W. T. Ruiz, Veerman, G., Vermorken, J. B., Peters, G. J., Bory, C., Chantin, C., Rocca, J. L., Bzowska, A., Ananlev, A. V., Ramzaeva, N., Alksins, E., Maurins, J. A., Kulikowska, E., Shugar, D., Davies, P. M., Fairbanks, L. D., Simmonds, H. A., De Graaf, T. W., Van Dijk, W., de Jong D., Huysmans F., De Abreu R., Monnens L., Dijkman H., van Liebergen F., Assmann K., Duley, J. A., Hanefeld, F., Fabianowska-Majewska, K., Duley, A. J., Greger, J., Wasiak, T., Gathof, B. S., Ried, T., Zwieauer, K., Gresser, U., Griesmacher, A., Weigel, G., Müller, M. M., Gross, M., Empl, W., Guisan, B., Roch-Ramel, F., Harley, E. H., Baumgarten, I., Kaletha K., Nagel-Starczynowska G., Karlsson, Anna, Lambooy, L., Boerbooms, A., De Abreu, R., van de Putte, L., Stolk, J., vd Pulte, L., Komissarov, A. A., Romanova, D. V., Debabov, V. G., Kozlov, A. M., Sokolov, V. B., Aksinenko, A. Y., Fetisov, V. I., Komoriya, Keiji, Osada, Yoshio, Hasegawa, Masaichi, Kondo, Shiro, Couch, Ronald C., Griffin, Travis B., Lakaschus, G., Löffler, H. G., Löffler, M., Jiménez, M. López, Salinero, M. A., Guilarte, J. Sánchez, Matesanz, J. Perianes, Medina, L. Vigil, Galiana, J. Ruiz, Marlewski, M., Smolenski, R. T., Swierczynski, J., Rutkowski, B., Zydowo, M. M., Micheli, V., Pescaglini, M., Sestini, S., Jacomelli, G., Magagnoli, C., Pompucci, G., Hayek, G., Mimouni, M., Boptemps, F., Van den Berghe, G., Miranda, M. E., Mateos, F. A., Herrero, E., González, A., Puig, J. G., Miscetti, P., Minelli, A., Proietti, A., Moroni, M., Mezzasoma, I., Moro, J. F., Noam, I., Cameron, J. S., McBride, M. B., Mathew, C. G. P., Ogg, C. S., Puig, J. G., Miranda, M. E., Mateos, F. A., Luzi, L., Noordhuis, P., Kazemier, K., Kaspers, G. J. L., Overgaard-Hansen, K., Marcussen, M., Klenow, H., Pelatti, A., Quaratino, C. P., D'Amario, C., Tentarelli, R., Giacomello, A., Rocchigiani, M., Iacomelli, G., Pandolfi, M. L., Arezzini, L., Terzuoli, L., Pagani, R., Di Sciascio, N., Colosimo, A., Ranieri-Paggi, M., Ronca, F., Brown, P. E., Moir, A. J. G., Raggi, A., Schwendel A., Grune T., Siems W. G., Holzhuetter H. G., Sebesta, I., Krijt, J., Vondrak, K., Kmoch, S., Hrebicek, M., Senatore, G., Del Lucchese, G., Martini, C., Lucacchini, A., Siems, W. G., Mertsch, K., Blasig, I., Grune, T., Skladanowski, A. C., Hoffmann, C. S., Krass, J. D., Makarewicz, W., Jastorff, B., Slingerland, R. J., Bodlaender, J. M., Van Lenthe, H., Elzinga, L., Van Kuilenburg, A. B. P., Voûte, P. A., Van Gennip, A. H., Smid, K., van der Wilt, C. L., Aherne, G. W., Pinedo, H. M., Sorqi, M. L., Rucci, C., Zoppini, A., Staub M., Sasvári-Székely M., Spasokukockaja T., Hrabák A., Stolk, J. N., De Abreu, R. A., De Koning, D. G. M., Lambooy, L. H. M., Kerstens, P. J. S. M., Boerbooms, A. M. Th., van de Putte, L. B. A., Szüts, P., Szirovicza, éva, åbrahám, C. S., Havass, Z., Jezewska, M. M., Van Acker, K. J., Eyskens, F. J., Verkerk, R. H., Scharpé, S. S., van Kuppevelt, T. H., Veerkamp, J. H., Sabina, R. L., Van Laar J. A. M., Mayhew E., Durrani F. A., Peters G. J., Rustum Y. M., Wang, J. Z., Leoncini, R., Vannoni, D., and Marinello, E.

Published
1993
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23. Modulation of Airway Epithelial Innate Immunity and Wound Repair by M(GM-CSF) and M(M-CSF) Macrophages

Author

van Riet, S, Schadewijk, A. (Annemarie) van, de Vos, S., Vandeghinste, N. (Nicky), Rottier, R.J. (Robbert), Stolk, J. (J.), Hiemstra, P.S. (Pieter), Khedoe, P., van Riet, S, Schadewijk, A. (Annemarie) van, de Vos, S., Vandeghinste, N. (Nicky), Rottier, R.J. (Robbert), Stolk, J. (J.), Hiemstra, P.S. (Pieter), and Khedoe, P.

Abstract

Airway epithelial cells and macrophages participate in inflammatory responses to external noxious stimuli, which can cause epithelial injury. Upon injury, epithelial cells and macrophages act in concert to ensure rapid restoration of epithelial integrity. The nature of the interactions between these cell types during epithelial repair is incompletely understood. We used an in vitro human coculture model of primary bronchial epithelial cells cultured at the air-liquid interface (ALI-PBEC) and polarized primary monocyte-derived macrophages. Using this coculture, we studied the contribution of macrophages to epithelial innate immunity, wound healing capacity, and epithelial exposure to whole cigarette smoke

Published
2020
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33. [alpha]sub-1-Antitrypsin deficiency o 7: Computed tomographic imaging in [alpha]sub-1-antitrypsin deficiency

Author

Shaker, S.B., Stavngaard, T., Stolk, J., Stoel, B., and Dirksen, A.

Subjects
Smoking -- Risk factors, Glycoproteins -- Health aspects, Glycoproteins -- Care and treatment, Glycoproteins -- Research, Emphysema, Pulmonary -- Development and progression, Emphysema, Pulmonary -- Research, Emphysema, Pulmonary -- Causes of, Emphysema, Pulmonary -- Diagnosis, Tomography, Quality of life, Pulmonary function tests, Health
Published
2004

38. The European Alpha-1 Research Collaboration (EARCO): a new ERS Clinical Research Collaboration to promote research in alpha-1 antitrypsin deficiency

Author

Miravitlles, M., Chorostowska-Wynimko, J., Ferrarotti, I., McElvaney, N.G., O'Hara, K., Stolk, J., Stockley, R.A., Turner, A., Wilkens, M., Greulichon, T., Corsico, A., Corda, L., Sucena, M., Barrecheguren, M., Esquinas, C., Parr, D., Lara, B., Mahadeva, R., Chlumsky, J., Janciauskiene, S., Bals, R., Seersholm, N., Kohler, M., Clarenbach, C., Altraja, A., Jenssens, W., Gouder, C., Hecimovic, A., Dudvarski, A., Krams, A., Ulmeanu, R., Zaharie, A., Mornex, J.F., Yorgancioglu, A., Schmid-Scherzer, K., Tanash, H., Mazulov, O., Ivanov, Y., and EARCO Clinical Res Collaboration

Subjects
Pulmonary and Respiratory Medicine, Oncology, medicine.medical_specialty, Biomedical Research, International Cooperation, Alpha (ethology), 03 medical and health sciences, 0302 clinical medicine, Internal medicine, alpha 1-Antitrypsin Deficiency, Pulmonary Medicine, Medicine, Humans, 030212 general & internal medicine, Prospective Studies, Registries, Program Development, Alleles, International research, Alpha 1-antitrypsin deficiency, business.industry, medicine.disease, Europe, Clinical research, Phenotype, 030228 respiratory system, alpha 1-Antitrypsin, business
Abstract

The European Alpha-1 Research Collaboration (EARCO) will promote international research in alpha-1 antitrypsin deficiencyhttp://ow.ly/DWwg30nwCj4

Published
2019

49. Liver fibrosis and metabolic alterations in adults with homozygous alpha1-antitrypsin deficiency (PiZZ genotype)

Author

Hamesch, K., primary, Woditsch, V., additional, Mandorfer, M., additional, Moeller, L.S., additional, Pereira, Vítor, additional, Delgado, M.P., additional, Reichert, M.C., additional, Heimes, C.V., additional, Voss, J., additional, Spivak, I., additional, Gueldiken, N., additional, Arslanow, A., additional, Schaefer, B., additional, Zoller, H., additional, Aigner, E., additional, Reiberger, T., additional, Martin, W., additional, Siegmund, B., additional, Eray, Y., additional, Danilo, B., additional, Stolk, J., additional, Gleiber, W., additional, Knipel, V., additional, Windisch, W., additional, Bals, R., additional, Koczulla, A.R., additional, Miravitlles, M., additional, Janciauskiene, S., additional, Genesca, J., additional, Lammert, F., additional, Trauner, M., additional, Krag, A., additional, Trautwein, C., additional, and Strnad, P., additional

Published
2018
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