Search

Your search keyword '"Sopoh, Ghislain E."' showing total 21 results
Start Over Author "Sopoh, Ghislain E." Publication Year Range Last 50 years
21 results on '"Sopoh, Ghislain E."'

3. Volatile organic compound detection of Buruli ulcer disease: Headspace analysis of Mycobacterium ulcerans and used gauzes of Buruli-compatible ulcers.

Author

Chudy, Stan F. J., Phanzu, Delphin M., Kolk, Arend H. J., Sopoh, Ghislain E., Barogui, Yves T., Tzfadia, Oren, Eddyani, Miriam, Fissette, Krista, de Jong, Bouke C., and Brinkman, Paul

Subjects
VOLATILE organic compounds, AIR sampling, UNIVARIATE analysis, METHYL cyclohexane, THERMAL analysis, BURULI ulcer
Abstract

Diagnosing Buruli ulcer (BU) is complicated by limited access to the sensitive IS2404 qPCR. Experienced clinicians report a distinct odour of Buruli ulcers. We explored the potential of headspace analysis by thermal desorption-gas chromatography-mass spectrometry to detect volatile organic compounds (VOCs) from Mycobacterium ulcerans both in vitro and clinically. This study was conducted in two phases: a discovery and validation phase. During the discovery phase, VOCs that enable identification of M. ulcerans cultures were determined. During the validation phase, these VOCs were evaluated in clinical samples for which we used gauzes from patients with skin ulcerations in the Democratic Republic of Congo. Seven M. ulcerans headspace samples were compared with four from sterile growth medium and laboratory environmental air. The univariate analysis resulted in the selection of 24 retained VOC fragments and a perfect differentiation between cultures and controls. Sixteen of 24 fragments were identified, resulting in eleven unique compounds, mainly alkanes. Methylcyclohexane was the best performing compound. Based on these 24 fragments, headspace samples originating from gauzes of 50 open skin lesions (12 qPCR positive and 38 negative) were analysed and an AUC of 0.740 (95%-CI 0.583–0.897) was obtained. As this is an experimental study, future research has to confirm whether the identified compounds can serve as novel biomarkers. Author summary: In this study the odour of bacteria that cause Buruli ulcer was examined. First, the air of bacteria cultured in the lab was analysed and compared with controls. Next, air was sampled from gauzes worn by people in the Kongo Central province, the Democratic Republic of Congo. Their lesions were compatible with Buruli ulcer disease but only 12 out of 50 were infected with the Buruli bacterium. In the odour of the Buruli bacteria and in gauzes from people with Buruli ulcer, several chemicals were present more abundantly than in gauzes from people with wounds caused by other diseases. Future research has to determine whether these chemicals can become part of a new test for Buruli ulcer disease. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

11. Subcutaneous Granulomatous Inflammation due to Basidiobolomycosis: Case Reports of 3 Patients in Buruli Ulcer Endemic Areas in Benin

Author

Brun, Luc V. C., Roux, Jean Jacques, Sopoh, Ghislain E., Aguiar, Julia, Eddyani, Miriam, Meyers, Wayne M., Stubbe, Dirk, Akele Akpo, Marie T., Portaels, Françoise, and de Jong, Bouke C.

Subjects
Article Subject
Abstract

Background. Basidiobolomycosis is a rare subcutaneous mycosis, which can be mistaken for several other diseases, such as soft tissue tumors, lymphoma, or Buruli ulcer in the preulcerative stage. Microbiological confirmation by PCR for Basidiobolus ranarum and culture yield the most specific diagnosis, yet they are not widely available in endemic areas and with varying sensitivity. A combination of histopathological findings, namely, granulomatous inflammation with giant cells, septate hyphal fragments, and the Splendore-Hoeppli phenomenon, can confirm basidiobolomycosis in patients presenting with painless, hard induration of soft tissue. Case Presentations. We report on three patients misdiagnosed as suffering from Buruli ulcer, who did not respond to Buruli treatment. Histopathological review of the tissue sections from these patients suggests basidiobolomycosis. All patients had been lost to follow-up, and none received antifungal therapy. On visiting the patients at their homes, two were reported to have died of unknown causes. The third patient was found alive and well and had experienced local spontaneous healing. Conclusion. Basidiobolomycosis is a rare subcutaneous fungal disease mimicking preulcerative Buruli ulcer. We stress the importance of the early recognition by clinicians and pathologists of this treatable disease, so patients can timely receive antifungal therapy.

Published
2018
Full Text
View/download PDF

12. Buruli ulcer treatment: Rate of surgical intervention differs highly between treatment centers in West Africa

Author

Wadagni, Anita C., primary, Steinhorst, Jonathan, additional, Barogui, Yves T., additional, Catraye, P. M., additional, Gnimavo, Ronald, additional, Abass, Kabiru M., additional, Amofa, George, additional, Frimpong, Michael, additional, Sarpong, Francisca N., additional, van der Werf, Tjip S., additional, Phillips, Richard, additional, Sopoh, Ghislain E., additional, Johnson, Christian R., additional, and Stienstra, Ymkje, additional

Published
2019
Full Text
View/download PDF

13. The paediatric participation scale measuring participation restrictions among former Buruli Ulcer patients under the age of 15 in Ghana and Benin: Development and first validation results

Author

Beeres, Dorien T., primary, Horstman, Jacolien, additional, van der Tak, Pierre, additional, Phillips, Richard O., additional, Abass, Kabiru M., additional, van der Werf, Tjip, additional, Johnson, Roch C., additional, Sopoh, Ghislain E., additional, de Zeeuw, Janine, additional, Dijkstra, Pieter U., additional, Barogui, Yves T., additional, and Stienstra, Ymkje, additional

Published
2019
Full Text
View/download PDF

15. Clinical Epidemiology of Buruli Ulcer from Benin (2005-2013): Effect of Time-Delay to Diagnosis on Clinical Forms and Severe Phenotypes

Author

Capela, Carlos, primary, Sopoh, Ghislain E., additional, Houezo, Jean G., additional, Fiodessihoué, René, additional, Dossou, Ange D., additional, Costa, Patrício, additional, Fraga, Alexandra G., additional, Menino, João F., additional, Silva-Gomes, Rita, additional, Ouendo, Edgard M., additional, Rodrigues, Fernando, additional, and Pedrosa, Jorge, additional

Published
2015
Full Text
View/download PDF

16. Persisting Social Participation Restrictions among Former Buruli Ulcer Patients in Ghana and Benin

Author

de Zeeuw, Janine, primary, Omansen, Till F., additional, Douwstra, Marlies, additional, Barogui, Yves T., additional, Agossadou, Chantal, additional, Sopoh, Ghislain E., additional, Phillips, Richard O., additional, Johnson, Christian, additional, Abass, K. Mohammed, additional, Saunderson, Paul, additional, Dijkstra, Pieter U., additional, van der Werf, Tjip S., additional, and Stientstra, Ymkje, additional

Published
2014
Full Text
View/download PDF

17. Psychometric Properties of the Participation Scale among Former Buruli Ulcer Patients in Ghana and Benin

Author

de Zeeuw, Janine, primary, Douwstra, Marlies, additional, Omansen, Till F., additional, Sopoh, Ghislain E., additional, Johnson, Christian, additional, Phillips, Richard O., additional, Alferink, Marike, additional, Saunderson, Paul, additional, Van der Werf, Tjip S., additional, Dijkstra, Pieter U., additional, and Stienstra, Ymkje, additional

Published
2014
Full Text
View/download PDF

20. Perceptions on the Effectiveness of Treatment and the Timeline of Buruli Ulcer Influence Pre-Hospital Delay Reported by Healthy Individuals

Author

Alferink, Marike, primary, van der Werf, Tjip S., additional, Sopoh, Ghislain E., additional, Agossadou, Didier C., additional, Barogui, Yves T., additional, Assouto, Frederic, additional, Agossadou, Chantal, additional, Stewart, Roy E., additional, Stienstra, Ymkje, additional, and Ranchor, Adelita V., additional

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results