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Cities and nations worldwide are pledging to energy and carbon neutral objectives that imply a huge contribution from buildings. High-performance targets, either zero energy or zero carbon, are typically difficult to be reached by single buildings, but groups of properly-managed buildings might reach these ambitious goals. For this purpose we need tools and experiences to model, monitor, manage and optimize buildings and their neighborhood-level systems. The paper describes the activities pursued for the deployment of an advanced energy management system for a multi-carrier energy grid of an existing neighborhood in the area of Milan. The activities included: (i) development of a detailed monitoring plan, (ii) deployment of the monitoring plan, (iii) development of a virtual model of the neighborhood and simulation of the energy performance. Comparisons against early-stage energy monitoring data proved promising and the generation system showed high efficiency (EER equal to 5.84), to be further exploited.

Objective: To analyse the development of the medical and surgical treatment of acute diverticulitis to develop an appropriate decision-making algorithm. Methods: We analysed the demographic characteristics, radiological images, disease severity, treatments and surgical outcomes of all of the patients with a diagnosis of acute diverticulitis admitted to the Department of General and Emergency Surgery between 1 January 2009 and 30 June 2014. Results: During the 66-month study period, 219 patients with acute diverticulitis attended our department; 69% had simple diverticulitis (93% were treated conservatively and 7% surgically) and 31% had complicated diverticulitis (76% were treated surgically and 24% conservatively). Of the patients who were treated surgically, 62.5% underwent primary resection with anastomosis, 31.94% Hartmann's procedure, and 5.56% laparoscopic lavage and drainage. Conclusions: Our cases and a careful review of the literature allowed us to develop a decision-making algorithm for patients with acute diverticulitis.

The aim of this study was to investigate the association between Cystic Fibrosis (CF) and affective temperaments, considering the relevance of ionic balances in neural excitability, as a possible neurobiological basis for temperamental expression. A cross-sectional study involving 55 adult CF patients was conducted. Sociodemographic, clinical and therapeutic characteristics, temperamental and personality dispositions and depressive and anxiety symptoms were evaluated through standardized semi-structured and structured interviews. The majority of the enrolled CF patients were receiving Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) therapy (72.7%), and most of them had hyperthymic temperament predominance (29.1%). Different TEMPS-A (Temperament Evaluation of Memphis, Pisa, Paris, and San Diego Autoquestionnaire) dimensions were not associated with the type of CF phenotype-related mutation or with the use of CFTR-modulator therapy. However, a tendency towards irritability was noted in patients not undergoing CFTR modulator therapy (6.7 ± 4.72 vs. 4.7 ± 4.33; p = 0.13). In light of the limitations imposed by the cross-sectional nature of the study, a hyperthymic temperament was found to be protective against current or lifetime psychopathologic events, whereas the other temperaments were associated with positive psychopathological anamnesis. Based on the measurement of temperament profiles and the study of their associations with clinically relevant variables, we argue that subjecting CF patients to such a temperament assessment could prove beneficial in the transition towards integrated and personalized care.

Introduction: Language is usually considered the social vehicle of thought in intersubjective communications. However, the relationship between language and high-order cognition seems to evade this canonical and unidirectional description (ie, the notion of language as a simple means of thought communication). In recent years, clinical high at-risk mental state (CHARMS) criteria (evolved from the Ultra-High-Risk paradigm) and the introduction of the Clinical Staging system have been proposed to address the dynamicity of early psychopathology. At the same time, natural language processing (NLP) techniques have greatly evolved and have been successfully applied to investigate different neuropsychiatric conditions. The combination of at-risk mental state paradigm, clinical staging system and automated NLP methods, the latter applied on spoken language transcripts, could represent a useful and convenient approach to the problem of early psychopathological distress within a transdiagnostic risk paradigm., Methods and Analysis: Help-seeking young people presenting psychological distress (CHARMS+/- and Clinical Stage 1a or 1b; target sample size for both groups n=90) will be assessed through several psychometric tools and multiple speech analyses during an observational period of 1-year, in the context of an Italian multicentric study. Subjects will be enrolled in different contexts: Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI), Section of Psychiatry, University of Genoa-IRCCS Ospedale Policlinico San Martino, Genoa, Italy; Mental Health Department-territorial mental services (ASL 3-Genoa), Genoa, Italy; and Mental Health Department-territorial mental services (AUSL-Piacenza), Piacenza, Italy. The conversion rate to full-blown psychopathology (CS 2) will be evaluated over 2 years of clinical observation, to further confirm the predictive and discriminative value of CHARMS criteria and to verify the possibility of enriching them with several linguistic features, derived from a fine-grained automated linguistic analysis of speech., Ethics and Dissemination: The methodology described in this study adheres to ethical principles as formulated in the Declaration of Helsinki and is compatible with International Conference on Harmonization (ICH)-good clinical practice. The research protocol was reviewed and approved by two different ethics committees (CER Liguria approval code: 591/2020-id.10993; Comitato Etico dell'Area Vasta Emilia Nord approval code: 2022/0071963). Participants will provide their written informed consent prior to study enrolment and parental consent will be needed in the case of participants aged less than 18 years old. Experimental results will be carefully shared through publication in peer-reviewed journals, to ensure proper data reproducibility., Trial Registration Number: DOI:10.17605/OSF.IO/BQZTN., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Problematic gaming is a topic of great clinical and social relevance, so it is particularly important to identify its protective and risk factors. Literature already showed that maladaptive personality favors problematic gaming. In the light of Kernberg's model of personality organization, the present cross-sectional study aimed to evaluate the mediational process through which low level of personality organization associates with problematic gaming, exploring the role of depressive and anxious symptoms and of motivations for playing. A sample of 1036 video gamers filled in an online survey regarding: level of personality organization; depressive and anxious symptoms; socializing, achievement, and immersion motivations for playing; problematic gaming. The path analysis showed that lower level of personality organization associated with greater problematic gaming and that depressive (but not anxious) symptoms, and socializing and achievement (but not immersion) motivations positively mediated this relationship. Furthermore, results showed that psychopathological symptoms and achievement motivations sequentially mediated the relationship between low level of personality organization and problematic gaming, highlighting that depressive symptoms secondary to a low level of personality organization could lead to a risky use of video games, i.e., they could push to use video games to self-enhance oneself, a dysfunctional strategy that exposes to problematic gaming. The study gives some indications on the variables that associate with problematic gaming and has some practical relevance, providing suggestions in the design of effective problematic gaming prevention and treatment interventions., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

A non-comparative multi-centre and international pilot study have been carried on Y-STRUT® (Hyprevention, France), an implantable medical device meant to reinforce the hip to reduce the risk of a contralateral hip fracture. Objectives of the study were to determine the feasibility and tolerance of the procedure. Methods Patients older than 60 years were recruited when presenting at the emergency departments with a low-energy pertrochanteric fracture on one side and with a fracture risk assessed for the contralateral side with BMD, T-Score or other bone quality evaluation tool, FRAX index, or fall risk assessment. Pain and functional ability were assessed at the different follow-up visits using VAS, WOMAC and OHS-12 scores. Results Twelve patients were included and reached a one-year follow-up. Mean age was 82 years old (65 - 91). The average hospital stay was 13 days (3 - 29). The prophylactic surgery did not delay the hospital discharge for any patient. The procedure did not lead to unresolvable serious adverse events. At 3 weeks, all patients were able to walk 6 meters, half of them in less of 30 seconds. Minimal pain was reported all along the follow-up visits, except at 3 years when one patient presented high pain in both hips. WOMAC and OHS-12 scores showed a moderate to mild hip impairment. Conclusion The good short and medium-term outcomes of this pilot study demonstrate the feasibility and the tolerability of the device. Further studies should focus on the efficacy of this immediate and lasting bone reinforcement technique.

Although mobile phones (MPs) are inexorably changing the forced migration experience, the realm of digital migration studies is still fragmented and lacking an analytical focus. Many research areas are still unexplored, while no narrative, scoping or systematic reviews have been conducted on this topic to date. The present review analyzed scientific contributions in Humanistic and Social Sciences with the aim to provide an overview of existing studies on the role of mobile phones (MPs) on refugees' experience, and to inform practice and policymaking for advancing the use of MPs for the protection of migrants' human rights. A scoping review was conducted using the Arksey and O'Malley framework and the JBI Reviewer's Manual recommendations. A three-step search was carried out in four bibliographic databases by three independent reviewers. Review selection and extraction were performed using an interactive team approach. Forty-three theoretical and empirical contributions were selected, and their content analyzed. The contributions ranged from 2013 to 2018 and varied in terms of disciplines, objectives, methodology, contexts, and migrants' origin, with the most studied group being Syrians. Five different topics concerning refugees' experience and MPs' usage emerged: (a) media practices in refugees' everyday lives; (b) opportunity and risks of MPs during the migration journey; (c) the role of MPs in maintaining and developing social relations; (d) potential of MPs for refugees" self-assertion and self-empowerment; (e) MPs for refugees' health and education. The results showed that modern devices, such as mobile phones, bring both risks and opportunities for refugees' experience, thereby both favouring and threatening asylum seekers' and refugees' human rights. Recommendations to policymaking and services and associations for advancing the use of MPs for the protection of the rights of migrants have been proposed., Competing Interests: The authors have declared that no competing interests exist.

Diazoalkane complexes [Os(η5-C5Me5)(N2CAr1Ar2)(PPh3){P(OR)3}]BPh4 (1, 2) [R = Me (1), Et (2); Ar1 = Ar2 = Ph (a); Ar1 = Ph, Ar2 = p-tolyl (b); Ar1Ar2 = C12H8 (fluorenyl) (c)] were prepared by reacting bromo-compounds OsBr(η5-C5Me5)(PPh3){P(OR)3} with an excess of diazoalkane in ethanol. The treatment of diazoalkane complexes 1 and 2 with acetylene under mild conditions (1 atm, RT) led to dipolar (3 + 2) cycloaddition affording 3H-pyrazole derivatives [Os(η5-C5Me5)(η1-[upper bond 1 start]N[double bond, length as m-dash]NC(C12H8)CH[double bond, length as m-dash]C[upper bond 1 end]H)(PPh3){P(OR)3}]BPh4 (6, 7) [R = Me (6), Et (7)] whereas reactions with terminal alkynes R1C[triple bond, length as m-dash]CH (R1 = Ph, p-tolyl, COOMe) gave vinylidene derivatives [Os(η5-C5Me5){[double bond, length as m-dash]C[double bond, length as m-dash]C(H)R1}(PPh3){P(OR)3}]BPh4 (8b-d, 9b-d) [R = Me (8), Et (9); R1 = Ph (b), p-tolyl (c), COOMe (d)]. Exposure to air of dichloromethane solutions of complexes 1 and 2 produced dioxygen derivatives [Os(η5-C5Me5)(η2-O2)(PPh3){P(OR)3}]BPh4 (10, 11) [R = Me (10), Et (11)]. Allenylidene [Os][double bond, length as m-dash]C[double bond, length as m-dash]C[double bond, length as m-dash]CR1R2 (12-14) [R1 = R2 = Ph (12, 13); R1 = Ph, R2 = Me (14)], vinylvinylidene [Os][double bond, length as m-dash]C[double bond, length as m-dash]C(H)C(Ph)[double bond, length as m-dash]CH2 (15) and 3-hydroxyvinylidene [Os][double bond, length as m-dash]C[double bond, length as m-dash]C(H)C(H)R2(OH) (16, 17) [R2 = Ph (16), H (17)] derivatives were also prepared. The vinylidene complex [Os(η5-C5Me5)([double bond, length as m-dash]C[double bond, length as m-dash]CH2)(PPh3){P(OMe)3}]BPh4 (8a) reacted with PPh3 to afford the alkenylphosphonium derivative [Os(η5-C5Me5){η1-C(H)[double bond, length as m-dash]C(H)PPh3}(PPh3){P(OMe)3}]BPh4 (18) whereas vinylidene complexes 8 and 9 reacted with water leading to the hydrolysis of the alkyne and the formation of carbonyl complexes [Os(η5-C5Me5)(CO)(PPh3){P(OR)3}]BPh4 (19, 20). The complexes were characterised by spectroscopic data (IR and NMR) and by X-ray crystal structure determination of [Os(η5-C5Me5){[double bond, length as m-dash]C[double bond, length as m-dash]C(H)p-tolyl}(PPh3){P(OEt)3}]BPh4 (9c), [Os(η5-C5Me5)(η2-O2)(PPh3){P(OMe)3}]BPh4 (10) and [Os(η5-C5Me5)(CO)(PPh3){P(OMe)3}]BPh4 (19).

Organic azide complexes [Os(η5-C5H5)(κ1-N3R)(PPh3){P(OR1)3}]BPh4 (1, 2) [R = CH2C6H5 (a), CH2C6H4-4-CH3 (b), CH(CH3)C6H5 (c), C6H5 (d); R1 = Me (1), Et (2)] were prepared by allowing bromo-compounds [OsBr(η5-C5H5)(PPh3){P(OR1)3}] to react first with AgOTf and then with an excess of azide in toluene. Benzylazide complexes reacted in solution leading to imine derivatives [Os(η5-C5H5){κ1-NH[double bond, length as m-dash]C(R2)Ar}(PPh3){P(OR1)3}]BPh4 (3, 4) [R2 = H (a, b), CH3 (c); Ar = C6H5, C6H4-4-CH3; R1 = Me (3), Et (4)]. Phenylazide, on the other hand, reacted in solution affording the dinuclear dinitrogen complex [{Os(η5-C5H5)(PPh3)[P(OMe)3]}2(μ-N2)](BPh4)2 (5). Depending on the nature of the R substituent, the reaction of the p-cymene complex [OsCl2(η6-p-cymene)(PPh3){P(OEt)3}] with RN3 yielded imine [OsCl(η6-p-cymene){κ1-NH[double bond, length as m-dash]C(H)Ar}{P(OEt)3}]BPh4 (6) (Ar = C6H4-4-CH3) and amine derivatives [OsCl(η6-p-cymene)(κ1-NH2C6H5){P(OEt)3}]BPh4 (7). The complexes were characterised spectroscopically (IR, 1H, 31P, 15N NMR) and by the X-ray crystal structure determination of [{Os(η5-C5H5)(PPh3)[P(OMe)3]}2(μ-N2)](BPh4)2 (5).

To valorize agricultural wastes and byproducts in southern Italy, anaerobic co-digestion of six feedstocks (citrus pulp, olive pomace, cattle manure, poultry litter, whey, and corn silage) was studied to produce biogas for renewable energy generation. Both batch and semi-continuous co-digestion approaches were adopted to carry out the investigation. The feedstocks were mixed at different percentages according to their availabilities in southern Italy. The batch anaerobic co-digestion demonstrated that six studied feedstock mixtures generated an average of 239 mL CH 4 /g VS loading without significant difference between each other, which concluded that the feedstock mixtures can be used for biogas production. Considering the feedstock availability of citrus pulp and olive pomace in Sicily, three feedstock mixtures with the highest volatile solids concentration of citrus pulp (42% citrus pulp, 17% corn silage, 4% cattle manure, 8% poultry litter, and 18% whey; 34% citrus pulp, 8% olive pomace, 17% corn silage, 4% cattle manure, 8% poultry litter, and 18% whey; and 25% citrus pulp, 16% olive pomace, 17% corn silage, 4% cattle manure, 8% poultry litter, and 18% whey, respectively) were selected to run the semi-continuous anaerobic digestion. Under the stabilized culture condition, the feed mixture with 42% citrus pulp, 17% corn silage, 4% cattle manure, 8% poultry litter, and 18% whey presented the best biogas production (231 L methane/kg VS loading/day). The corresponding mass and energy balance concluded that all three tested feedstock mixtures have positive net energy outputs (1.5, 0.9, and 1.2 kWh-e/kg dry feedstock mixture, respectively)., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

β-Barrel shaped membrane proteins are attractive hosts for hybrid catalysts in which reactions are controlled through space. Production and extraction of β-barrel shaped membrane proteins in gram scale is challenging due to their hydrophobicity. Solvent mixtures such as chloroform/methanol (CM) are widely used for membrane protein extraction but toxicity and mutagenicity were reported in several cases. 2-Methyltetrahydrofuran (2-MeTHF) and cyclopentylmethylether (CPME) are two green (reduction of solvent-related environmental damage in chemical production) and potentially efficient solvents for membrane protein purification. On the example of the ferric hydroxamate uptake protein component A (FhuA) a 4-Step method was developed to provide gram amounts of highly purified FhuA: cell disruption (Step 1), removal of membrane protein impurities with n-octyl-poly-oxyethylene (oPOE) (Step 2), dissolution of membranes and FhuA precipitation (Step 3), and refolding using urea and dialysis with polyethylene-polyethyleneglycol (PE-PEG; Step 4) resulted in high FhuA purity (95% 2-MeTHF, 80% CPME; 70mg FhuA per liter fermenter broth). Structural integrity of FhuA protein was confirmed by circular dichroism (CD) and a translocation functionality assay., (Copyright © 2013 Elsevier B.V. All rights reserved.)

The asymmetric reduction of ketones is performed by using lyophilized whole cells in neat substrates with defined water activity (a(w)). Ketones and alcohols prone to be unstable in aqueous media can now be converted via biocatalysis.