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OBJECTIVE To compare a ventral and a left lateral endoscopic approach to coelioscopy in bearded dragons (Pogona vitticeps). ANIMALS 18 adult bearded dragons. PROCEDURES In a randomized crossover design involving 2 surgical approaches, anesthetized bearded dragons first underwent coelioscopy with a ventral approach (left lateral of midline next to the umbilicus; animal positioned in dorsal recumbency) or left lateral approach (intercostal; animal positioned in right lateral recumbency) and then with the alternate approach. A 2.7-mm × 18-cm, 30° oblique telescope with a 4.8-mm operating sheath and CO2 insufflation at 2 to 5 mm Hg were used. Ease of entry into the coelom and ease of visual examination of visceral structures were scored. RESULTS Both approaches were straightforward, with the left lateral approach requiring significantly more time than the ventral approach. Scores for ease of visual examination for the heart, lungs, liver, stomach, intestines, pancreas, gallbladder, left kidney, gonads, and fat body were good to excellent. Visual examination of the spleen and adrenal glands was difficult in most animals via either approach. The left kidney, testis, and vas deferens were easier to see with the left lateral approach, whereas the pancreas in females and gallbladder in both sexes were easier to see with the ventral approach. All bearded dragons recovered without complications from the procedures, except for one with nephritis, renal gout, and hepatic necrosis. CONCLUSIONS AND CLINICAL RELEVANCE Both coelioscopy approaches could be safely and effectively used in bearded dragons. Choice of approach should be based on the coelomic structures requiring evaluation.

Lipid bilayers are the basic structural components of all living systems, forming the membranes of cells, sub-cellular organelles, and extracellular vesicles. A class of man-made lipidic vesicles called multilobed magnetic liposomes (MMLs) is reported in this work; these MMLs possess a previously unattained combination of features owing to their unique multilobe structure and composition. MMLs consist of a central cluster of lipid-coated magnetic iron oxide nanoparticles that lend them a magnetophoretic velocity comparable to the most efficient living microswimmers. Multiple liposome-like lobes protrude from the central region; these can incorporate both water-soluble and lipid-soluble molecular payloads at high carrying capacity and exchange the incorporated substances with the membranes of both artificial and live cells by the contact diffusion mechanism. The size of MMLs is controllable in the range of 200-800 nm. Their functionality is demonstrated by completing a model mission where MMLs are remotely controlled to collect, transport, and deliver a cargo to live cells.

Pigment cell tumors, also known as chromatophoromas, are cutaneous spindle cell neoplasms originating from pigment cells (chromatophores) in the dermis of teleosts, amphibians, and reptiles. Chromatophoromas share similar histologic morphology to other spindle cell tumors and are not always pigmented. Therefore, immunohistochemical analysis may be useful in distinguishing these neoplasms from tumors of other cellular origin when poorly pigmented. We performed 3 immunohistochemistry assays (PNL-2, melan A, and SOX10) on 8 cutaneous neoplasms from 8 teleosts diagnosed as chromatophoromas based on histologic morphology. Semiquantitative analysis of immunoreactivity was evaluated on each immunohistochemical assay using a 0–3 scale. PNL-2 exhibited mild-to-moderate (1 or 2) immunoreactivity in 7 of the cases, and resident chromatophores (internal control) were also immunoreactive in these cases. Melan A exhibited mild-to-moderate (1 or 2) immunoreactivity in 4 cases (and with resident chromatophores in these cases); SOX10 was not immunoreactive in any cases. Our results indicate that PNL-2 may be a useful marker in teleosts to distinguish tumors of chromatophore origin. Melan A could also be useful, but appears to be less sensitive, and SOX10 is likely not a useful marker for these neoplasms in teleosts.

Limited physiological measurements exist for the digestive physiology of porcupines. We measured CH4 emission in three captive Indian crested porcupines (Hystrix indica; 16.1 ± 2.7 kg) fed a diet of pelleted lucerne, and measured feed intake, digestibility, and digesta mean retention time (MRT) of a solute and three particle markers (

To compare a ventral and a left lateral endoscopic approach to coelioscopy in bearded dragons (18 adult bearded dragons.In a randomized crossover design involving 2 surgical approaches, anesthetized bearded dragons first underwent coelioscopy with a ventral approach (left lateral of midline next to the umbilicus; animal positioned in dorsal recumbency) or left lateral approach (intercostal; animal positioned in right lateral recumbency) and then with the alternate approach. A 2.7-mm × 18-cm, 30° oblique telescope with a 4.8-mm operating sheath and COBoth approaches were straightforward, with the left lateral approach requiring significantly more time than the ventral approach. Scores for ease of visual examination for the heart, lungs, liver, stomach, intestines, pancreas, gallbladder, left kidney, gonads, and fat body were good to excellent. Visual examination of the spleen and adrenal glands was difficult in most animals via either approach. The left kidney, testis, and vas deferens were easier to see with the left lateral approach, whereas the pancreas in females and gallbladder in both sexes were easier to see with the ventral approach. All bearded dragons recovered without complications from the procedures, except for one with nephritis, renal gout, and hepatic necrosis.Both coelioscopy approaches could be safely and effectively used in bearded dragons. Choice of approach should be based on the coelomic structures requiring evaluation.

In contrast to mammalian herbivores, birds are generally perceived to produce little methane (CH4) during digestion, and accounting for poultry in greenhouse gas inventories is considered unnecessary. We measured CH4 emissions in six domestic geese (Anser anser, 5.0 ± 0.9 kg) and six domestic turkeys (Meleagris gallopavo, 6.3 ± 0.6 kg) kept on a diet of lucerne pellets only, using open-circuit chamber respirometry. Measurements of oxygen consumption were similar to previously published values in these species. Absolute CH4 emissions per day were lower in geese (0.58 ± 0.10 L) than in turkeys (1.48 ± 0.16 L) and represented 0.4 ± 0.2 and 0.6 ± 0.1% of gross energy intake, respectively. These results confirm previous findings on the presence of methanogenes in the digestive tract of poultry species, and in vitro measurements performed on poultry caecal contents. In relation to mammalian herbivores in terms of absolute CH4 emissions, CH4 yield per dry matter or gross energy intake, or the CH4:CO2 ratio, the lucerne-fed geese and turkeys had comparatively low values. The emission of CH4 in spite of the very short digesta retention times and low fibre digestibility, as measured in the same animals, gives rise to the hypothesis that that in some birds, caecal fermentation and the associated CH4 production may be related to the microbial digestion of uric acid. The hypothesis that CH4 emissions in poultry may depend not only on dietary fibre but also on dietary digestible protein (that is excreted as uric acid in urine and retrogradely transported from the cloaca into the caeca) remains to be tested.

Methane (CH4) production is a ubiquitous, apparently unavoidable side effect of fermentative fibre digestion by symbiotic microbiota in mammalian herbivores. Here, a data compilation is presented of in vivo CH4 measurements in individuals of 37 mammalian herbivore species fed forage-only diets, from the literature and from hitherto unpublished measurements. In contrast to previous claims, absolute CH4 emissions scaled linearly to DM intake, and CH4 yields (per DM or gross energy intake) did not vary significantly with body mass. CH4 physiology hence cannot be construed to represent an intrinsic ruminant or herbivore body size limitation. The dataset does not support traditional dichotomies of CH4 emission intensity between ruminants and nonruminants, or between foregut and hindgut fermenters. Several rodent hindgut fermenters and nonruminant foregut fermenters emit CH4 of a magnitude as high as ruminants of similar size, intake level, digesta retention or gut capacity. By contrast, equids, macropods (kangaroos) and rabbits produce few CH4 and have low CH4 : CO2 ratios for their size, intake level, digesta retention or gut capacity, ruling out these factors as explanation for interspecific variation. These findings lead to the conclusion that still unidentified host-specific factors other than digesta retention characteristics, or the presence of rumination or a foregut, influence CH4 production. Measurements of CH4 yield per digested fibre indicate that the amount of CH4 produced during fibre digestion varies not only across but also within species, possibly pointing towards variation in microbiota functionality. Recent findings on the genetic control of microbiome composition, including methanogens, raise the question about the benefits methanogens provide for many (but apparently not to the same extent for all) species, which possibly prevented the evolution of the hosting of low-methanogenic microbiota across mammals.

The digestive physiology of maras (Dolichotis patagonum) has not been investigated in detail. Maras have a particular limb anatomy facilitating a unique cursoriality among rodents. This may also have led to additional adaptations such as a reduced volume of the gastrointestinal tract. We performed macroanatomical measurements of, and determined mean particle size along, the digestive tract of 10 semi-free-ranging animals (7.04 ± 1.05 kg). Additionally, we measured CH4 emission in five captive animals (7.67 ± 0.98 kg) fed a diet of pelleted lucerne, and measured food intake, digestibility, and digesta mean retention time (MRT) of a solute and three particle markers (fed at < 2, 10 and 20 mm particle size). The digestive tract contents represented 11.1 ± 1.4% of body mass, similar to other mammals and rodents, and there was slight indication of selective small particle retention in the caecum. Secondary peaks in marker elimination patterns suggested the possibility of caecotrophy. The MRTs were 15.4 h for the solute and 13.6 h, 13.3 h and 13.3 h for the three particle markers, respectively. At a dry matter intake of 61 ± 12 g kg body mass-0.75 d−1, the maras digested organic matter and neutral detergent fibre to 48 ± 8% and 34 ± 10%, respectively, which is in the lower range of results from horses fed on a diet with a similar fibre content. The respiratory quotient (CO2/O2) was 0.93 ± 0.03, the resting metabolic rate 346 ± 35 kJ kg body mass-0.75 d−1, and CH4 emissions averaged at 3.85 ± 0.47 L d−1 and 14.5 ± 5.2 L per kg dry matter intake; this at a CH4/CO2 ratio of 0.042 ± 0.004. Thus, the methane yield was of a magnitude expected for a hypothetical ruminant of this body mass. The results are consistent with the general understanding of hystricomorph rodent digestive physiology, including caecotrophy, but do not indicate a reduction of digestive capacity to support cursoriality. These results, and those obtained from other hystricomorph rodents, suggest that CH4 production may be more prominent in rodents than previously thought.

Although it is known that most herbivores produce methane (CH4), CH4 emissions in rodents are generally considered negligible and have rarely been measured in live animals. We measured CH4 emission in four captive juvenile nutria (Myocastor coypus) fed a diet of pelleted lucerne, as well as food intake, digestibility, and digesta mean retention time (MRT) of a solute and a particle marker. Marker excretion patterns revealed secondary peaks indicative of coprophagy, with MRTs of 30.2 ± 4.2 h and 24.2 ± 4.2 h for solutes and particles, respectively, and a resulting MRTsolute/MRTparticle ratio of 1.26 ± 0.07, which is still typical for a ‘mucus-trap’ colonic separation mechanism. At a dry matter intake of 28 ± 6 g kg body mass−0.75 d−1, the nutria digested organic matter and neutral detergent fibre at 59 ± 3% and 46 ± 3%, respectively, similar to what might be expected from horses on a diet with this fibre content. The respiratory quotient (CO2/O2) was 0.95 ± 0.02, the resting metabolic rate 266 ± 31 kJ kg body mass−0.75 day−1 and CH4 emissions averaged at 1.72 ± 0.17 L day−1 and 39.8 ± 11.3 L per kg dry matter intake; this at a CH4/CO2 ratio of 0.08 ± 0.04. Accordingly, methane yield was of a magnitude expected for a hypothetical ruminant of this body mass. While rodents’ CH4 contributions to global budgets might be low due to their low body size, this should not give rise to the assumption that CH4 production is not a relevant part of their digestive physiology.

Ostriches (Struthio camelus) are herbivorous birds with a digestive physiology that shares several similarities with that of herbivorous mammals. Previous reports, however, claimed a very low methane emission from ostriches, which would be clearly different from mammals. If this could be confirmed, ostrich meat would represent a very attractive alternative to ruminant—and generally mammalian—meat by representing a particularly low-emission agricultural form of production. We individually measured, by chamber respirometry, the amount of oxygen consumed as well as carbon dioxide and methane emitted from six adult ostriches (body mass 108.3 ± 8.3 kg) during a 24-hour period when fed a pelleted lucerne diet. While oxygen consumption was in the range of values previously reported for ostriches, supporting the validity of our experimental setup, methane production was, at 17.5 ± 3.2 L d− 1, much higher than previously reported for this species, and was of the magnitude expected for similar-sized, nonruminant mammalian herbivores. These results suggest that methane emission is similar between ostriches and nonruminant mammalian herbivores and that the environmental burden of these animals is comparable. The findings furthermore indicate that it appears justified to use currently available scaling equations for methane production of nonruminant mammals in paleo-reconstructions of methane production of herbivorous dinosaurs.

We describe cases of collyriclosis in apodiform and passeriform birds in Portugal, Switzerland, and Germany. We extend the host range of Collyriculm faba to include apodiform birds ( Apus apus , Apus melba , and Apus pallidus ) and the passerine Sitta europaea (Eurasian Nuthatch). Infections varied in severity from an incidental finding to severe debilitation and death. The infection route remains unclear with the apparent absence from Germany, Portugal, and Switzerland of the first intermediate host of C. faba, the aquatic gastropod Bythinella austriaca, implying that other organisms might be involved in the parasite's life cycle. Furthermore, the detection of C. faba cysts in very young passerine birds may indicate an infection during the nestling stage and a rapid development of parasite-containing subcutaneous cysts. This series of cases highlights an increased geographic range into Portugal and the potential debilitating nature of a parasite of migratory birds in Europe. However, given the rarity of cases, collyriclosis does not seem to present an important threat to migratory species preservation. publishersversion published

Although it is well-known that retrograde transport of urine fills the caeca of birds with fluid and small particles, the function of avian caeca is still not fully understood. We measured mean retention times (MRT) of solute (cobalt-EDTA, Co), small particle (< 2 mm, chromium-mordanted fibre, Cr) and large particle (8 mm, cerium-marked fibre, Ce) markers in geese (Anser anser) and turkeys (Meleagris gallopavo) fed alfalfa pellets ad libitum. Intake did not differ between species. Turkeys had longer MRT for all markers (Co: 10.4 vs. 3.2 h; Cr 23.3 vs. 2.9 h; Ce 9.5 vs. 2.1 h), achieved a higher fibre digestibility, and had a higher calculated dry matter gut fill. Thus, geese and turkeys correspond to the typical dichotomy of good fliers vs. poor fliers/flightless species in avian herbivores. Because uric acid is fermented much faster by microbes than fibre, the ultimate cause of short-MRT digesta retention in avian caeca as in geese is possibly rather uric acid than fibre fermentation. The numerical differences between marker MRT in geese correspond to a colonic separation mechanism that delays the excretion of fluids more than small and again more than large particles. In contrast to geese, turkeys excreted solid and liquid (caecal) faeces. Liquid faeces contained less fibre and more crude protein than solid faeces and accounted for the excretion of 7, 25 and 34% of Ce, Co and Cr markers. Marker excretion patterns and MRT for liquid faeces (Co 15 vs. Cr 50 h) suggest that small particles did not simply move in parallel to fluids, but were retained selectively by being trapped in colonic digesta upon expulsion from caeca, with subsequent repeated retrograde transport into caeca with the next batch of urine. Given the absence of coprophagy in birds (in contrast to small mammalian herbivores), such a delay of small (microbial) particle escape from the caeca appears reasonable.

Methane (CH4) production varies between herbivore species, but reasons for this variation remain to be elucidated. Here, we report open-circuit chamber respiration measurements of CH4 production in four specimens each of two non-ruminant mammalian herbivores with a complex forestomach but largely differing in body size, the collared peccary (Pecari tajacu, mean body mass 17kg) and the pygmy hippopotamus (Hexaprotodon liberiensis, 229kg) fed lucerne-based diets. In addition, food intake, digestibility and mean retention times were measured in the same experiments. CH4 production averaged 8 and 72L/d, 18 and 19L/kg dry matter intake, and 4.0 and 4.2% of gross energy intake for the two species, respectively. When compared with previously reported data on CH4 production in other non-ruminant and ruminant foregut-fermenting as well as hindgut-fermenting species, it is evident that neither the question whether a species is a foregut fermenter or not, or whether it ruminates or not, is of the relevance previously suggested to explain variation in CH4 production between species. Rather, differences in CH4 production between species on similar diets appear related to species-specific differences in food intake and digesta retention kinetics.

Ratites differ in the anatomy of their digestive organs and their digesta excretion patterns. Ostriches (Struthio camelus) have large fermentation chambers and long digesta retention, emus (Dromaius novaehollandiae) have a short gut and short retention times, and rheas (Rhea americana) are intermediate. A recent study showed that ostriches produce as much methane (CH4) as expected for a similar-sized, non-ruminant mammalian herbivore. We hypothesized that emus and rheas produce less CH4 than ostriches. We individually measured, by chamber respirometry, the amount of O2 consumed as well as CO2 and CH4 emitted from six adult rheas (body mass 23.4 ± 8.3 kg) and two adult emus (33.5 and 32.0 kg) during 23-hour periods on a pelleted lucerne diet. In contrast to previous studies, which classified emus as non-producers, we measured CH4 emissions at 7.39 and 6.25 L/day for emus and 2.87 ± 0.82 L/day for rheas, which is close to values expected for similar-sized non-ruminant mammals for both species. O2 consumption was of a similar magnitude as reported previously. Across ratites CH4 yield (L/kg dry matter intake) was positively correlated with mean retention time of food particles in the gut, similar to findings within ruminant species. In ratites, this relationship leads to similar body mass-specific CH4 production for a high intake/short retention and a low intake/long retention strategy. Therefore, when investigating CH4 production in herbivorous birds, it is advisable to consider various CH4 measures, not only yield or absolute daily amount alone.

Ratites differ distinctively in the anatomy of their digestive tract. For example Ostriches (Struthio camelus) have a particularly long voluminous colon and long paired caeca Rheas (Rhea spp.) are characterised by a short colon with particularly prominent paired caeca and Emus (Dromaius novaehollandiae) – have neither very prominent caeca nor a prominent colon. We tested whether digesta excretion patterns corresponded to these differences in anatomy expecting Ostriches to have the longest and Emus the shortest digesta retention times and Rheas possibly showing a selective retention of fluids observed in other birds and mammals with prominent caeca. We used 6 Ostriches (97 123kg) 5 Greater Rheas (R. americana 22 27kg) and 2 Emus (32 34kg) fed a common diet of alfalfa pellets ad libitum in captivity. Intake per unit of metabolic body mass did not differ between Ostriches and Rheas but was significantly higher in Emus which also displayed higher defecation frequencies and lower fiber digestibility. Mean digesta retention time for small fiber particles (2 mm) differed significantly among species (Ostrich: 30 36h; Rhea: 7 19h; Emu: 1.3 1.8h) but there were no differences between the retention of 2 mm or 8 mm particles or a solute marker within species. The shape of the marker excretion curves corresponded to digesta mixing in the digestive tract of Ostriches and Rheas but not Emus. The calculated dry matter gut fill ( of body mass) was significantly higher in Ostriches (1.6 1.8) than Rheas (0.3 1.0) and Emus (0.2). Ostriches had the highest and Emus the lowest fecal dry matter concentration. These physiological findings match the differences in digestive anatomy and support the concept that in ratites herbivory – and hence flightlessness – evolved repeatedly in different ways.

Sloths are renowned for their low metabolic rate, low food intake and low defecation frequency. We investigated factors of digestive physiology and energy metabolism in four captive individuals (mean body mass 10.0 ± SD 3.7 kg) of a hitherto mostly unstudied sloth species, Linné's two-toed sloth (Choloepus didactylus), in a 2-week digestion recording and 23-h respiration experiment on animals fed a standard zoo diet of vegetables and starchy components. Dry matter intake, defecation frequency and particle mean retention time (MRT) in the gastrointestinal tract (GIT) were 12 ± 3 g/(kg(0.75) day), once every 5 days and140 h in three individuals, but 53 g/(kg(0.75) day), daily and 82 h in one individual that was apparently compensating for a period of weight loss prior to the experiment. In all animals, solute marker was eliminated at a faster rate than the particle marker, indicating 'digesta washing' in the sloths' GIT. The overall metabolic rate calculated from oxygen consumption matched the metabolisable energy intake in three individuals [173 ± 22 vs. 168 ± 44 kJ/(kg(0.75) day)] but not in the fourth one [225 vs. 698 kJ/(kg(0.75) day)], supporting the interpretation that this animal was replenishing body stores. In spite of the low food intake and the low-fibre diet (209 ± 26 g neutral detergent fibre/kg dry matter), methane production was rather high accounting for 9.4 ± 0.8% of gross energy intake (2.7% in the fourth individual), which exceeded literature data for ruminants on forage-only diets. These results corroborate literature reports on low intake, low defecation frequency, low metabolic rate and long MRT in other sloth species. The long MRT is probably responsible for the comparatively high methane production, providing more opportunity for methanogenic archaea than in other non-ruminant mammals to produce significant amounts of methane.