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Abstract Around 13% of fossil fuels globally are used for non-combustion purposes. Fossil fuel processing plants, such as petroleum refineries, exhibit interdependent material and energy system dynamics, making the transition away from fossil fuels in energy systems more challenging without addressing the non-energy outputs. This study explores the future role of fossil fuels for non-energy purposes in climate-stringent scenarios with restrictions on alternative feedstock availability, focusing on the primary chemicals sector. Using a global integrated assessment model with detailed refining and primary chemicals sectors, findings across various scenarios reveal that up to 62% of total feedstock use in the chemical sector could be provided by alternative sources by 2050. This would require significant scale-up in biomass utilisation and carbon capture technologies. Annual CO2 emissions from the chemical sector could be reduced to as low as −1Gt CO2 by the same year if carbon storage in non-recycled and non-incinerated bioplastics is accounted for.

Saccharomyces boulardii has emerged as a promising probiotic agent in bolstering the immune system. In vaccinology, its application can be explored to optimize vaccine efficacy by augmenting host immune response and consequently enhancing the immunogenicity of antigenic formulations. However, it is imperative to conduct further research to comprehend the effectiveness of this probiotic in novel vaccines targeting significant pathogens affecting animal health. Hence, this study investigated the effects of a short (3 - 5 days) or continuously (56 days) S. boulardii supplementation (3 × 108 CFU/mL) on the adaptive immunity of sheep immunized with a recombinant antigen against Paeniclostridium sordellii (rAPS). Four immunized groups (G1-G4) were evaluated, varying the regimen of S. boulardii supplementation. Elevated levels of anti-rAPS IgG immunoglobulins were detected in all vaccinated animals. Daily supplementation with S. boulardii (G1) resulted in higher IgG levels, reaching antibody titers of up to 25,600, which were 16 times higher than those observed in not supplemented group (G4) and 4 times higher than in the group supplemented for 3 days (G3) or 5 days (G2) before each immunization. These findings demonstrate that S. boulardii can enhance vaccine-induced immune responses against P. sordellii, particularly IgG-mediated immune responses in sheep. The possibility of a short supplementation for 5–3 days is a very important finding for animal husbandry, considering the supplementation and supply management cost.

Integrated Assessment Models (IAMs) are cornerstone tools in guiding decarbonization policies. However, the petrochemical sector, which is crucial to the future of oil and gas due to its non-energy fossil feedstock demands, has been overlooked in IAMs. This study aims to evaluate the implications of this under-representation on decarbonization strategies. We assessed the Illustrative Mitigation Pathways from the Intergovernmental Panel on Climate Change in its Sixth Assessment Report. Our analysis indicates that, without stringent material policies, the feedstock demand can reach unattainable levels from 2050 onwards. This is evidenced by the required disruptive yields of primary oil in feedstocks, which exceed 80 % in some cases. The findings highlight the necessity for improved modelling of the petrochemical sector within IAMs.

Abstract Since the inception of COVID-19 pandemic, there has been a challenging race for the development of precise diagnostic tests. Specific SARS-CoV-2 serological assays are the main tools used to estimate the rate of past infections or herd immunity in epidemiological studies, in addition to being helpful in guiding public health management policies. In this study, an in-house ELISA based on the construct of SARS-CoV-2 nucleocapsid (N) proteins, named rCoV2, rCoV4, and rCoV7, showed diagnostic performance for the detection of IgG antibodies. Sensitivity was evaluated in serum samples from patients with mild to moderate or severe COVID-19 infections, which were collected at different time points, while specificity was evaluated using pre-pandemic sera. In samples from mild to moderate cases obtained ≥16 days after the onset of symptoms, the sensitivities for rCoV2, rCoV4, and rCoV7 were 66.7%, 75%, and 77.8%, respectively. For samples from severe cases, the sensitivity was above 80% for all constructs. All proteins showed high specificity (94-98%). Overall, rCoV7 (C-terminus N-protein portion) showed better diagnostic performance, with 62.3% sensitivity in moderate and severe cases and 96.6% specificity. The SARS-CoV-2 ELISA using N-protein-based constructs could be a promisor tool for investigate the epidemiology of COVID-19 and monitor population-level serosurveillance.

Lateral flow immunoassays (LFIA) for antibody detection represent cost-effective and user-friendly tools for serology assessment. This study evaluated a new LFIA prototype developed with a recombinant chimeric antigen from the spike/S and nucleocapsid/N proteins to detect anti-SARS-CoV-2 IgG antibodies. The evaluation of LFIA sensitivity and specificity used 811 serum samples from 349 hospitalized, SARS-CoV-2 RT-qPCR positive COVID-19 patients, collected at different time points and 193 serum samples from healthy controls. The agreement between ELISA results with the S/N chimeric antigen and LFIA results was calculated. The LFIA prototype for SARS-CoV-2 using the chimeric S/N protein demonstrated 85 % sensitivity on the first week post symptoms onset, reaching 94 % in samples collected at the fourth week of disease. The agreement between LFIA and ELISA with the same antigen was 92.7 %, 0.827 kappa Cohen value (95 % CI [0.765–0.889]). Further improvements are needed to standardize the prototype for whole blood use. The inclusion of the novel chimeric S + N antigen in the COVID-19 IgG antibody LFIA demonstrated optimal agreement with results from a comparable ELISA, highlighting the prototype's potential for accurate large-scale serologic assessments in the field in a rapid and user-friendly format.

Resumo A síndrome de Rendu-Osler-Weber, também conhecida como telangiectasia hemorrágica hereditária, é uma doença hereditária autossômica dominante. Ela é caracterizada pela presença de múltiplas malformações arteriovenosas e telangiectasias. Este artigo relata dois casos de pacientes com síndrome de Rendu-Osler-Weber que apresentaram malformações arteriovenosas pulmonares e foram submetidos a tratamento endovascular com sucesso. Uma breve revisão da literatura mostra que até 50% dos pacientes com a síndrome têm malformações arteriovenosas pulmonares e geralmente há um histórico familiar positivo nesses pacientes. Em 30% dos casos, elas são múltiplas e estão associadas a complicações mais graves da doença. A maioria dos pacientes é assintomática, mesmo na presença de malformações arteriovenosas com shunt direito-esquerdo. Quando esses shunts excedem 25% do volume total de sangue, podem surgir dispneia, cianose, baqueteamento digital e sopros extracardíacos. O tratamento endovascular oferece segurança e controle das complicações da telangiectasia hemorrágica hereditária, sendo atualmente o tratamento de escolha para essas lesões.

The inclusion of Sustainable Development Goals (SDGs) in climate mitigation pathways is critical and can be reached by assessing their consequences through the deployment of appropriate indicators to that end. Integrated Assessment Models (IAMs) are important tools for understanding possible impacts caused by adopting new policies. We investigate terrestrial biodiversity trends (life on land: SDG 15) for three different climate mitigation scenarios for Brazil: (1) A scenario compatible with a world that maintains its current policies with current deforestation rates in the Amazon and the Cerrado biomes; (2) A scenario in which Brazil fulfils its Nationally Determined Contribution (NDC); and (3) A scenario compatible with a world that limits warming to 1.5°C. We use the Brazilian Land-Use and Energy System model (BLUES), a national IAM, to show the implications of the transitions involved in the above-mentioned scenarios for the country up to 2050. We conduct a post-processing analysis using consolidated biodiversity indicators to emphasize how different IAM greenhouse gas (GHG) emissions mitigation solutions present distinct positive and negative potential impacts on biodiversity in Brazil. However, our analysis does not consider the impacts associated with climate change, but only the risks imposed by mitigation policies. Our results indicate that biodiversity loss decreases in the scenarios from (1) to (3), implying that stronger climate change mitigation actions could result in smaller biodiversity losses. We conclude that Brazil has the opportunity to align its biodiversity and climate goals through nature-based solutions (NBS), such as forest conservation, restoration, pasture recovery, and the use of crop-pasture and agroforestry systems.

This ethnographic paper deals with the phenomenon of transmission of family jewelry and its effects, as a production of ancestry. It is the result of a broader research in which I show that a family jewel is not a ordinary jewel; it is a jewel « in the family », as it is constituted in connection with affective groups. Its transfers tend to transit vertically, propagate narratives and signal guardianship: it must be preserved, cared for, exhibited, recounted, and passed on. Moreover, family jewelry is a sacred and inalienable asset, entrusted to the person who cannot dispose of it freely. Because of its economic value, it is subject to the selling taboo, a device implemented to keep it in the family line.

Abstract Improvements in modelling energy systems of populous emerging economies are highly decisive for a successful global energy transition. The models used–increasingly open source–still need more appropriate open data. As an illustrative example, we take the Brazilian energy system, which has great potential for renewable energy resources but still relies heavily on fossil fuels. We provide a comprehensive open dataset for scenario analyses, which can be directly used with the popular open energy system model PyPSA and other modelling frameworks. It includes three categories: (1) time series data of variable renewable potentials, electricity load profiles, inflows for the hydropower plants, and cross-border electricity exchanges; (2) geospatial data on the administrative division of the Brazilian federal states; (3) tabular data, which contains power plant data with installed and planned generation capacities, aggregated grid network topology, biomass thermal plant potential, as well as scenarios of energy demand. Our dataset could enable further global or country-specific energy system studies based on open data relevant to decarbonizing Brazil’s energy system.

Abstract Bioenergy could play a major role in decarbonizing energy systems in the context of the Paris Agreement. Large‐scale bioenergy deployment could be related to sustainability issues and requires major infrastructure investments. It, therefore, needs to be studied carefully. The Bioenergy and Land Optimization Spatially Explicit Model (BLOEM) presented here allows for assessing different bioenergy pathways while encompassing various dimensions that influence their optimal deployment. In this study, BLOEM was applied to the Brazilian context by coupling it with the Brazilian Land Use and Energy Systems (BLUES) model. This allowed investigating the most cost‐effective ways of attending future bioenergy supply projections and studying the role of recovered degraded pasture lands in improving land availability in a sustainable and competitive manner. The results show optimizing for limiting deforestation and minimizing logistics costs results in different outcomes. It also indicates that recovering degraded pasture lands is attractive from both logistics and climate perspectives. The systemic approach of BLOEM provides spatial results, highlighting the trade‐offs between crop allocation, land use and the logistics dynamics between production, conversion, and demand, providing valuable insights for regional and national climate policy design. This makes it a useful tool for mapping sustainable bioenergy value chain pathways.

Resumo A fístula arteriovenosa (FAV) é uma sequela incomum de rotura espontânea de aneurisma arterial dentro do sistema venoso adjacente. Descrevemos, em um paciente de 74 anos, o tratamento endovascular de FAV ilíaca à direita por aneurisma de artéria ilíaca comum (AIC) roto associado a aneurisma de AIC distal à esquerda, em cuja cirurgia foram preservadas as artérias lombares e mesentérica inferior por necessidade de excluir simultaneamente as artérias hipogástricas. Foram demonstrados os fenômenos na dinâmica do balanço hídrico ocorridos em decorrência da interrupção da FAV. A evolução do paciente foi benigna, com normalização das graves alterações hemodinâmicas que apresentava e com desaparecimento dos sintomas respiratórios atribuídos à hipertensão arterial pulmonar.

This work is aiming at estimating the radiation doses to members of the public in case of an emergency exposure situation related to a hypothetical uranium mining tailings dam rupture at the UDC - Decommissioning Unit of Caldas, MG, Brazil. The calculations will help to managing the exposures of emergency workers and residents in the region downstream the dam. The computer code chosen to perform the simulations was FLO-2D@ software. Due to the uncertainties involved, mainly regarding the waste rheology and activity concentrations and in parameters values to be used in the dose assessment, considerations were made for the study to present results derived from conservative assumptions. Through the creation of flood maps, it was possible to estimate the extent of the areas affected by the released materials. The results showed that in a scenario of the tailings dam breach, deposition of mud shall extend over 20 km downstream affecting an area of nearly 3 km2. Total dose for a member of the public living in most affected areas would be around 43 mSv/y in the early phase after the accident. Although only shielding would be recommended under current radiation protection regulation, evacuation/relocation is suggested due to the presence of long-lived radionuclides. This protective measure would probably already be indicated by the accident itself, regardless of radiological reasons. More realistic assessment should be performed before returning people, considering long-term environmental transport and uses of the area. It must also be considered that long-term exposures have more restricted recommended acceptable levels than those for the emergency phase.

The severe acute respiratory syndrome originated by the new coronavirus (SARS-CoV-2) that emerged in late 2019, known to be a highly transmissible and pathogenic disease, has caused the COVID-19 global pandemic outbreak. Thus, diagnostic devices that help epidemiological public safety measures to reduce undetected cases and isolation of infected patients, in addition to significantly help to control the population’s immune response to vaccine, are required. To address the negative issues of clinical research, we developed a Diagnostic on a Chip platform based on a disposable electrochemical biosensor containing laser-induced graphene and a protein (SARS-CoV-2 specific antigen) for the detection of SARS-CoV-2 antibodies. The biosensors were produced via direct laser writing using a CO2 infrared laser cutting machine on commercial polyimide sheets. The presence of specific antibodies reacting with the protein and the K3[Fe(CN)6] redox indicator produced characteristic and concentration-dependent electrochemical signals, with mean current values of 9.6757 and 8.1812 µA for reactive and non-reactive samples, respectively, proving the effectiveness of testing in clinical samples of serum from patients. Thus, the platform is being expanded to be measured in a portable microcontrolled potentiostat to be applied as a fast and reliable monitoring and mapping tool, aiming to assess the vaccinal immune response of the population.

Summary: Aviation and shipping account for 22% of total transport-related CO2 emissions. Low-carbon fuels (such as biofuels and e-fuels) are the most promising alternatives to deeply decarbonize air and maritime transport. A number of technological routes focused on the production of renewable jet fuel can coproduce marine fuels, emulating the economies of scope of crude oil refineries. This work aims to investigate possible synergies in the decarbonization of aviation and shipping in Brazil, selected as an interesting case study. An Integrated Assessment Model (IAM) of national scope is used to explore different combinations of sectoral and national climate targets. This IAM represents not only the energy supply and transport systems but also the agricultural and land-use systems. In the absence of a deep mitigation policy for Brazil, results indicate synergies related to oilseed- and lignocellulosic-based biofuels production routes. Imposing a strict carbon budget to the Brazilian economy compatible with a world well below 2°C, the portfolio of aviation and shipping fuels changes significantly with the need for carbon dioxide removal strategies based on bioenergy. In such a scenario, synergies between the two sectors still exist, but most renewable marine energy supply is a by-product of synthetic diesel produced for road transport, revealing a synergy different from the one originally investigated by this work.

Listeria monocytogenes is the causative agent of listeriosis, a highly lethal disease initiated after the ingestion of Listeria-contaminated food. This species comprises different serovars, from which 4b, 1/2a, and 1/2b cause most of the infections. Among the different proteins involved in pathogenesis, the internalins A (InlA) and B (InlB) are the best characterized, since they play a major role in the enterocyte entry of Listeria cells during early infection. Due to their covalent attachment to the cell wall and location on the bacterial surface, along with their exclusive presence in the pathogenic L. monocytogenes, these proteins are also used as detection targets for this species. Even though huge advancements were achieved in the enrichment steps for subsequent Listeria detection, few studies have focused on the improvement of the antibodies for immunodetection. In the present study, recombinant InlA and InlB produced in Escherichia coli were used as targets to generate antibodies via phage display using the human naïve antibody libraries HAL9 and HAL10. A set of five recombinant antibodies (four against InlA, and one against InlB) were produced in scFv-Fc format and tested in indirect ELISA against a panel of 19 Listeria strains (17 species; including the three main serovars of L. monocytogenes) and 16 non-Listeria species. All five antibodies were able to recognize L. monocytogenes with 100% sensitivity (CI 29.24–100.0) and specificity (CI 88.78–100.0) in all three analyzed antibody concentrations. These findings show that phage display-derived antibodies can improve the biological tools to develop better immunodiagnostics for L. monocytogenes.

The assessment of protective and remediation measures for rural areas is more complex than that for urban areas, due to the strong influence of large number of parameters and variables associated with climate, diet, eating habits, farming practices and the type of soil in the area. Thus, it is not possible to perform generic studies applicable to any kind of area. Specific studies and surveys should be made in the areas most likely to be contaminated due to a nuclear accident. Considering the Brazilian Nuclear Power Central, the prioritization of areas was based on radiological and economic aspects. This preliminary study demonstrated that the different soil types in Brazil are more relevant to the ingestion dose than the regional differences in the diets. The most relevant product for public dose was identified as the ingestion of milk. The contribution of milk to total ingestion dose depends on the season of the year when the accident occurs. The timing of the application of the measure has an important effect on the percentage dose reduction that can be achieved. In particular for I-131, protective measures related to milk ingestion must be considered within the emergency phase in order to be effective.

To evaluate the effectiveness of current national policies in achieving global temperature targets is important but a systematic multi-model evaluation is still lacking. Here the authors identified a reduction of 3.5 GtCO2 eq of current national policies relative to a baseline scenario without climate policies by 2030 due to the increasing low carbon share of final energy and the improving final energy intensity.

Conventional oil reserves in Colombia are depleted. The country´s reserve-to-production ratio is estimated as 5 years. Therefore, the search for new resources and their conversion into proven reserves are essential. In this case, the production of unconventional reservoirs is an option in Colombia. This work evaluates the technical and economic feasibility of the production of a Tight Oil source rock reservoir, considering parameters such as fracture shape factor, fracture propagation, fracture pressure, international oil price, petrophysical characteristics, fluid properties, drilling cost, completion, and fiscal regime. The methodological development of the work allowed concluding that this reservoir located in the middle Magdalena Valley basin has production potential and those factors such as the type of completion, drilling technique, and cost of lifting the resource have a significant impact on the viability of the project.

This work presents an Integrated Assessment Model (IAM) developed for Ecuador, the so-called Ecuador Land Use and Energy Network Analysis model (ELENA). This model includes six distinctive sectors of the economy and displays the four geographic regions composing the country. The model enables to capture sectorial interactions, under a set of scenarios designed to evaluate the energy and land perspectives until 2050. The model is a crucial planning instrument to evaluate public policies, such as National Determined Contributions (NDC) and even more ambitious decarbonisation scenarios. Findings show that Ecuador's NDC are not aligned with the “well below” 2 °C target, committed in the Paris Agreement. Moreover, to achieve deep decarbonisation it is necessary to endorse disruptive strategies in which bioenergy and reforestation play a main role. To keep under the 1.5 °C temperature threshold above pre-industrial levels, Ecuador's energy matrix must be diversified with higher shares of low carbon technologies and electrification of energy end use in the transport, buildings and industry sectors. Biomass with carbon capture and storage (BECCS) and biofuels could transform the energy sector in a CO2 sink.

A exposição à radiação natural é a principal fonte de exposição média para a população mundial. O objetivo deste trabalho é efetuar estimativas de dose externa na população brasileira decorrentes desta exposição. Para o cálculo de doses efetivas, foram utilizados os modelos utilizados pelo United Nations Scientific Committee for the Effects of Atomic Radiation – UNSCEAR. Em relação à radiação cósmica, foi considerada a exposição ao nível do mar em função da latitude, corrigida para a altitude dos locais selecionados, que compreendem os municípios brasileiros com uma população maior do que 100.000 habitantes, o que inclui 53% da população do país e pelo menos uma cidade em cada unidade da federação. Para o cálculo da radiação terrestre, os locais foram limitados àqueles para os quais existem informações sobre medidas de taxa de dose ou de concentração de radionuclídeos naturais no solo. Estes locais incluem apenas 21% da população do país. Os resultados para a exposição à radiação cósmica são semelhantes àqueles estimados pelo UNSCEAR como médias mundiais. Os resultados para a exposição à radiação terrestre são bastante superiores àqueles indicados como média mundial. Este resultado pode refletir a preferência por estudos radioecológicos em áreas de alta radiação natural de fundo.

Abstract Background The avian infectious bronchitis virus (IBV) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. This study examined the combined use of an ELISA and Western blot (WB) to detect antibodies against the nucleocapsid protein (N) of IBV. The coding sequence for N was amplified by RT-PCR and expressed in Escherichia coli. A soluble recombinant N protein (rN) of approximately 50 kDa was obtained. A total of 389 sera were tested against the rN in ELISA and the results were compared with those of the commercial IDEXX IBV Ab test. ELISA-rN achieved a 90.34% sensitivity and 90.16% specificity. WB confirmed all false negative sera in ELISA-rN or IDEXX test as truly positive. The current study indicate that the combined use of rN in ELISA and WB is a powerful tool for the immunodiagnosis of avian infectious bronchitis. Methods Constructed recombinant pAE/n expression vectors were used to transform E. coli BL21(DE3) Star competent cells (Invitrogen). The rN of infectious bronchitis virus was purified by affinity chromatography using HisTrap HP 1 mL columns pre-packed with pre-charged Ni Sepharose in the ÄKTAprime Automated Liquid Chromatography system (GE Healthcare). A total of 389 serum samples from chickens were used to develop and evaluate the ELISA-rN test. To standardize the indirect ELISA development, serum dilutions (1:100, 1:200 and 1:400) and different concentrations of purified rN antigen (50, 100 and 200 ng/well) were tested. Positive and negative sera for IBV were used as controls. The results were compared with those obtained from a commercial kit. Serum samples scored as negative with the commercial kit but as positive with the ELISA-rN were further analysed by Western blot analyses using the rN protein as an antigen. The results of the ELISA-rN were compared to the commercial kit results using receiver-operating characteristics curves, area under the curve, and confidence intervals with the software GraphPad Prism version 6.0 for Windows (GraphPad Software, USA). Results The expected cDNA fragment of approximately 1240 bp was successfully amplified by PCR using primers designed to select for the coding region of the N protein. The rN was expressed as a soluble protein to avoid the refolding steps and, after purification a yield of 10 mg/L of rN was obtained. The SDS-PAGE results demonstrated the presence of two distinct bands that had a molecular mass of approximately 45 and 50 KDa. Out of 244 sera that scored positive in the commercial ELISA IDEXX IBV Ab Test, 220 were also positive in the ELISA-rN, yielding an ELISA-rN test sensitivity of 90.16%. Out of 145 sera that scored negative in the IDEXX IBV Ab Test, 131 also scored negative in the ELISA-rN, indicating a specificity of 90.34%. Sera that tested negative in the ELISA-rN and positive in the commercial test also reacted with the rN protein in Western blot. Conclusions The association between the ELISA and Western blot techniques developed in this study with a subunit of IBV (rN) were able to detect antibodies that the commercial ELISA did not detect suggesting that the ELISA-rN has greater sensitivity.

Este texto resulta de um exercício etnográfico realizado junto a seguidores brasileiros da moda urbana Lolita, estilo japonês criado na década de 1970 e inspirado no vestuário de bonecas vitorianas combinado com elementos da cultura pop nipônica. A estética se difundiu a partir dos anos 1990. Exploro a experiência de vestir Lolita e, através desta, a percepção de si e a produção de individualidades associadas à performance de gênero. Procuro demonstrar como esta indumentária específica reitera o excesso como signo do feminino, mesmo quando usada sobre corpos de homens.

BackgroundCOVID-19 is characterized by a rapid change in the patient's condition, with major changes occurring over a few days. We aimed to develop and evaluate an emergency system for monitoring patients with COVID-19, which may be useful in hospitals where more severe patients stay in their homes.Methodology/principal findingsThe system consists of the home-based patient unit, which is set up around the patient and the hospital unit, which enables the medical staff to telemonitor the patient's condition and help to send medical recommendations. The home unit allows the data transmission from the patient to the hospital, which is performed using a cell phone application. The hospital unit includes a virtual instrument developed in LabVIEW® environment that can provide a real-time monitoring of the oxygen saturation (SpO2), beats per minute (BPM), body temperature (BT), and peak expiratory flow (PEF). Abnormal events may be fast and automatically identified. After the design details are described, the system is validated by a 30-day home monitoring study in 12 controls and 12 patients with COVID-19 presenting asymptomatic to mild disease. Patients presented reduced SpO2 (pConclusionsAn emergency system for home monitoring of patients with COVID-19 was developed in the current study. The proposed system allowed us to quickly respond to early abnormalities in these patients. This system may contribute to conserving hospital resources for those most in need while simultaneously enabling early recognition of patients under acute deterioration, requiring urgent assessment.

Abstract Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products. As an alternative, yeast such as Pichia pastoris have secretory mechanisms, which could diminish the cost and time for production. This study aimed to produce recombinant proteins in Pichia pastoris and verify their sensibility and specificity in an indirect ELISA assay. Two sequences (rTES-30 and rTES-120) of Toxocara canis excretory-secretory antigens were cloned in a pPICZαB vector and expressed in P. pastoris KM71H. Sera samples collected from human adults infected by Toxocara spp. were tested by indirect ELISA using rTES-30 and rTES-120 as antigens. Recombinant proteins were detected at 72 hours after induction, in the supernatant, as pure bands between 60~70 kDa with hyperglycosylation. Regarding diagnosis potential, recombinant antigens had high specificity (95.6%); however, sensitivity was 55.6% for rTES-30 and 68.9% for rTES-120. Further deglycosylation of the P. pastoris antigens did not seem to affect ELISA performance (p>0.05). The low sensitivity in the serodiagnosis diminished any advantage that P. pastoris expression could have. Therefore, we do not recommend P. pastoris recombinant TES production as an alternative for the diagnosis of toxocariasis.

Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the proteins LigA and LigB of Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni strain Fiocruz L1-130. The humoral immune response was evaluated by indirect ELISA. Cytokine expression levels were determined by RT-qPCR. Compared to the control group, the IgG antibody levels of animals immunized with pTARGET/ligAni and pTARGET/ligBrep plasmids associated with xanthan biopolymer were significantly higher than the control group. Additionally, there was a significant increase in IL-17 expression in animals vaccinated with pTARGET/ligBrep and xanthan.

This synthesis paper presents the objectives, approach and cross-cutting results of the Latin American Deep Decarbonization Pathways project (DDP-LAC). It synthesizes and compares detailed national and sectoral deep decarbonization pathways (DDPs) to 2050 compatible with the Paris Agreement objectives and domestic development priorities in Argentina, Colombia, Costa Rica, Ecuador, Mexico and Peru. The first five countries analysed in detail the energy system and agriculture, forestry and land use (AFOLU) at a high level, while Peru focussed on a detailed analysis of AFOLU given its predominance in its GHG emissions. While economy-wide results were produced, this paper focuses on the electricity, passenger transport, and AFOLU results because of their current emissions, potential to grow, and identification of successful strategies for decarbonization (e.g. switching to clean electricity and other net-zero emissions fuels across the economy; urban planning, mode shifting, and electrification in passenger transport; and intensive sustainable agriculture, assignment of land use rights and their enforcement and afforestation in AFOLU). It also highlights where significant emissions remain in 2050, notably in industry, AFOLU, freight, and oil and gas production, all areas for future research. It derives insights for the design of domestic policy packages and identifies priorities for international cooperation. This analysis provides critical information for Long-Term Strategies, Nationally Determined Contributions and Global Stocktaking in the context of the Paris Agreement.

A exposição à radiação cósmica em viagens de avião é significativamente maior do que a exposição ao nível do solo e varia de acordo com a rota, a altitude e o tempo de voo. O programa CARI-6, desenvolvido pela Administração Federal de Aviação dos EUA, efetua o cálculo da dose efetiva de radiação cósmica galáctica recebida por um indivíduo em um avião a voar a rota mais curta entre dois aeroportos do mundo. O objetivo deste projeto é o de estimar a contribuição de exposição à radiação cósmica em voos comerciais domésticos para a população brasileira. Um banco de dados, incluindo cerca de 4.000 voos domésticos no Brasil, foi implementado em planilhas do Excel com base em informações de voos para novembro de 2011. O banco de dados descreve a origem e destino de cada voo, hora de partida e chegada, o tipo de avião, número de passageiros, as horas de voo (incluindo períodos de decolagem, pouso e altitude de cruzeiro) e número de voos por ano. Doses individuais para voos domésticos no Brasil variaram de 1,8 a 8,8 μSv. Considerando-se o limite ocupacional de 850 h de voo por ano para tripulantes e números de voos de cada rota, a dose ocupacional média seria de cerca de 0,76 mSv/y. Doses coletivas, para o número total de voos por ano foram estimadas em 429 e 22 homem-Sv/y para os passageiros e membros da tripulação, respectivamente.

O objetivo deste trabalho foi apresentar, avaliar e elaborar representações gráficas para complementar a segunda edição do Guia Alimentar para a População Brasileira e facilitar a educação nutricional. Trata-se de um estudo qualitativo, participativo e quantitativo. O delineamento amostral utilizado foi o não probabilístico. Mediante as diretrizes dietéticas do guia alimentar elaborou-se as representações gráficas. O ícone/símbolo escolhido para elaborá-las foi a Bandeira do Brasil. Para a avaliação das mesmas foi utilizada a técnica de grupo focal. Realizou-se análise qualitativa e estatística descritiva. Os grupos focais foram compostos de 2% (n=2) nutricionistas docentes da Universidade do Estado de Minas Gerais (UEMG), unidade de Passos-MG, 81% (n=83) acadêmicos do Curso de Nutrição da UEMG e 17% (n=18) clientes/pacientes do Centro de Atendimento Nutricional (CAN) da UEMG. A média de idade foi de 27 ± 12 anos, com mínimo de 18 e máximo de 62 anos, sendo 83% (n=85) do sexo feminino. Da avaliação qualitativa, destaca-se que os participantes compreenderam as associações apresentadas (cores, proporções e categorias de alimentos) e conseguiram identificar o que é uma alimentação saudável e de risco por meio das representações gráficas. Quando da avaliação quantitativa, a maioria dos participantes apontou que o material apresentado melhorou os conceitos sobre o que é uma alimentação e hábitos saudáveis de vida (96%; n=98). Além disso, 98% (n=101) relataram que seria importante as pessoas conhecerem e terem acesso (97%; n=99) ao material. Conclui-se que as representações gráficas poderão complementar a nova edição do guia alimentar e facilitar a educação nutricional. ABSTRACT Feeding guide for the brazilian population: complementation through graphical representations The aim of this work was to present, assess and elaborate graphical representations to complement the second edition of the Food Guide for the Brazilian Population and facilitate nutrition education. This is a qualitative study, quantitative and participatory. The sample design used was the not probabilistic. Using the dietary guidelines of the food guide was developed the graphical representations. The icon/symbol chosen for drafting was the flag of Brazil. For the evaluation of the same was used the focal group technique. Qualitative analysis and descriptive statistics was conducted. The focal groups were composed of 2% (n = 2) nutrition teachers of the University of the State of Minas Gerais (USMG), unit of Passos-MG, 81% (n = 83) students of nutrition course of USMG and 17% (n = 18) clients/patients of the Nutritional Care Center (NCC) USMG. The median age was 27 ± 12 years, with a minimum of 18 and maximum of 62 years, being 83% (n = 85) female. About the qualitative assessment, the participants understood the associations presented (colors, proportions and food categories) and were able to identify what is a healthy diet and a risk diet by means of graphical representations. Concerning the quantitative evaluation, the mostly of participants pointed out that the material presented improved the concepts about what is food and healthy habits for life (96%; n = 98). In addition, 98% (n = 101) reported that it is important that more people knew and had access to the material (97%; n = 99). It is concluded that the graphical representations may complement the new edition of the food guide and facilitate nutrition education.

Emissions pathways after COVID-19 will be shaped by how governments’ economic responses translate into infrastructure expansion, energy use, investment planning and societal changes. As a response to the COVID-19 crisis, most governments worldwide launched recovery packages aiming to boost their economies, support employment and enhance their competitiveness. Climate action is pledged to be embedded in most of these packages, but with sharp differences across countries. This paper provides novel evidence on the energy system and greenhouse gas (GHG) emissions implications of post-COVID-19 recovery packages by assessing the gap between pledged recovery packages and the actual investment needs of the energy transition to reach the Paris Agreement goals. Using two well-established Integrated Assessment Models (IAMs) and analysing various scenarios combining recovery packages and climate policies, we conclude that currently planned recovery from COVID-19 is not enough to enhance societal responses to climate urgency and that it should be significantly upscaled and prolonged to ensure compatibility with the Paris Agreement goals.