12 results on '"Raissa R. Christoff"'
Search Results
2. The Dynamics of Axon Bifurcation Development in the Cerebral Cortex of Typical and Acallosal Mice
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Pamela Meneses Iack, Roberto Lent, Christiane Bonifácio, Danielle Rayêe, Raissa R. Christoff, Patricia P. Garcez, Jürgen Boltz, and Michele R. Lourenço
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Cerebral Cortex ,Mice, Inbred BALB C ,Placenta ,General Neuroscience ,Dynamics (mechanics) ,Guidepost cells ,Commissure ,Biology ,Corpus callosum ,Embryonic stem cell ,Axons ,Corpus Callosum ,Mice ,Dysgenesis ,medicine.anatomical_structure ,nervous system ,Pregnancy ,Cerebral cortex ,medicine ,Animals ,Female ,Axon ,Neuroscience - Abstract
The corpus callosum (CC) is a major interhemispheric commissure of placental mammals. Early steps of CC formation rely on guidance strategies, such as axonal branching and collateralization. Here we analyze the time-course dynamics of axonal bifurcation during typical cortical development or in a CC dysgenesis mouse model. We use Swiss mice as a typical CC mouse model and find that axonal bifurcation rates rise in the cerebral cortex from embryonic day (E)17 and are reduced by postnatal day (P)9. Since callosal neurons populate deep and superficial cortical layers, we compare the axon bifurcation ratio between those neurons by electroporating ex vivo brains at E13 and E15, using eGFP reporter to label the newborn neurons on organotypic slices. Our results suggest that deep layer neurons bifurcate 32% more than superficial ones. To investigate axonal bifurcation in CC dysgenesis, we use BALB/c mice as a spontaneous CC dysgenesis model. BALB/c mice present a typical layer distribution of SATB2 callosal cells, despite the occurrence of callosal anomalies. However, using anterograde DiI tracing, we find that BALB/c mice display increased rates of axonal bifurcations during early and late cortical development in the medial frontal cortex. Midline guidepost cells adjacent to the medial frontal cortex are significant reduced in the CC dysgenesis mouse model. Altogether these data suggest that callosal collateral axonal exuberance is maintained in the absence of midline guidepost signaling and might facilitate aberrant connections in the CC dysgenesis mouse model.
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- 2021
3. Contributors
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Tawfik Aboellail, Jônatas Santos Abrahão, Talita Adelino, Ramesh Akkina, Ayman Alboudi, Luiz Carlos Junior Alcantara, Henning Andersen, Teresinha De Jesus Aguiar Dos Santos Andrade, Masashi Arakawa, Josélio Maria Galvão de Araújo, Pamella Nunes Azevedo, Omar Bagasra, Mark J. Bailey, Serdar Baraklı, Alison Jane Basile, Luis Federico Bátiz, Benan Bayrakci, Aline Almeida Bentes, Jean A. Bernatchez, A.B. Blázquez, Viola Borchardt-Lohölter, Ana Luiza Vilela Borges, Maria Sole Burali, Felipe A. Bustamante-Barrientos, Paulo E. Cabral Filho, Steven Vargas Cañas, Talita Castro, Gwong-Jen J. Chang, Day-Yu Chao, Ameya Chaudhari, Duverney Chaverra-Rodriguez, Raissa R. Christoff, Luiz Felipe Leomil Coelho, Diogo Goulart Corrêa, Michael Coste, Raquel Zanatta Coutinho, Joaquim Soares da Costa Júnior, Anna Carolina Toledo da Cunha Pereira, Paulo Marcos da Matta Guedes, Guilherme Liberato da Silva, Jaderson Costa DaCosta, Prajakta Dandekar, Amos Danielli, Maria das Dores Alves de Oliveira, Orhan Deniz, Philippe Desprès, Betânia Paiva Drumond, Jonny Duque, Patrícia e Silva Alves, Chaker El Kalamouni, José Veríssimo Fernandes, Gustavo Portela Ferreira, T. Foiadelli, Vagner Fonseca, Adriana Fontes, Lawrence Frenkel, Gilles Gadea, Patricia P. Garcez, Marta Giovanetti, Fernando Gomez, Bonnie E. Gulas-Wroblewski, Şadiye Gümüşyayla, Sunam Gurung, Juliano G. Haddad, Thomas Harbo, Cecília Hedin-Pereira, Roberto Henzi, Holly R. Hughes, Luiz Celso Hygino da Cruz Júnior, Andrew Jameson, Rachel Jordan, Rebecca B. Kairis, Taruna Kaura, Selman Kesici, Ahmad Suhail Khazali, Julia Maria Klemens, Erna Geessien Kroon, Walter Sze Tung Lam, Erik Lattwein, Vladimir V. Lazarev, Túlio César Rodrigues Leite, Cui Li, Nerilson Marques Lima, Maria Elizabeth Lopes Moreira, Karina Carrillo Loza, Denise Cantarelli Machado, Fernanda Majolo, Luiz Cosme Cotta Malaquias, Giuseppe Manfroni, Daniel Rodrigo Marinowic, Dimitri Marques Abramov, G.L. Marseglia, Ewen McLean, Breno de Mello Silva, Stelia Mendez-Sanchez, Abhishek Mewara, Eiji Morita, Kristy O. Murray, Dean Myers, Manuela Sales Lima Nascimento, Osvaldo J.M. Nascimento, Marciano Viana Paes, James Papin, Giovannia A.L. Pereira, Goreti Pereira, Maria I.A. Pereira, Naveed Pervaiz, Byron W. Purse, Kíssila Rabelo, Jéssika F.F. Ribeiro, Shannon E. Ronca, Shira Roth, Tania Regina Saad Salles, Lívia Sacchetto, J.C. Saiz, Natália Gedeão Salomão, Beate S. Santos, Sandra Saschenbrecker, S. Savasta, Wolfgang Schlumberger, Kimberly Schmitt, Jair L. Siqueira-Neto, Derek Tuck Loong Soon, José Luis Soto-Hernández, Gabriel Augusto Pires de Souza, Katja Steinhagen, Konstanze Stiba, Paul Ananth Tambyah, Gene S. Tan, Stephane Tosta, C. Trabatti, Gönül Vural, Heron Werner, Joilson Xavier, Zhiheng Xu, Tay Wei Xuan, and Rohana Yusof
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- 2022
4. Congenital Zika Virus Infection Impairs Corpus Callosum Development
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Raissa R. Christoff, Jefferson H. Quintanilha, Raiane O. Ferreira, Jessica C. C. G. Ferreira, Daniel M. Guimarães, Bruna Valério-Gomes, Luiza M. Higa, Átila D. Rossi, Janaina M. Vasconcelos, João L.S.G. Vianez, Maria Bellio, Amilcar Tanuri, Roberto Lent, and Patricia P. Garcez
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
5. Congenital Zika virus infection impairs corpus callosum development
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Átila Duque Rossi, Amilcar Tanuri, Luiza M. Higa, Raiane Oliveira Ferreira, Roberto Lent, Daniel M. Guimarães, Jéssica de Cassia Cavalheiro Gomes Ferreira, Patricia P. Garcez, João L.S.G. Vianez, Janaina M. Vasconcelos, Bruna Valério-Gomes, Jefferson H. Quintanilha, Raissa R. Christoff, and Maria Bellio
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Pathology ,medicine.medical_specialty ,Microcephaly ,Neurogenesis ,Biology ,medicine.disease ,Corpus callosum ,biology.organism_classification ,Axonogenesis ,Zika virus ,medicine.anatomical_structure ,nervous system ,medicine ,Axon guidance ,Axon ,Ventriculomegaly - Abstract
Congenital Zika Syndrome (CZS) is a set of birth defects caused by Zika virus (ZIKV) infection during pregnancy. Microcephaly is its main feature, but other brain abnormalities are found in CZS patients, such as ventriculomegaly, brain calcifications, and dysgenesis of the corpus callosum. Many studies have focused on microcephaly, but it remains unknown how ZIKV infection leads to callosal malformation. To tackle this issue, we infected mouse embryos in utero with a Brazilian ZIKV isolate and found that they are born with a reduction in callosal area and density of callosal neurons. ZIKV infection also causes a density reduction of PH3+ cells, intermediate progenitor cells and SATB2+ neurons. Moreover, axonal tracing revealed that callosal axons are reduced and misrouted. Also, ZIKV infected cultures show a reduction of callosal axon length. GFAP labelling showed that in utero infection compromises glial cells responsible for midline axon guidance. The RNA-Seq data from infected brains identified downregulation of axon guidance and axonogenesis related genes. In sum, we showed that ZIKV infection impairs critical steps of corpus callosum formation by disrupting not only neurogenesis but also axon guidance and growth across the midline.Summary StatementZika virus infection during development impairs the formation of corpus callosum by disturbing axon guidance and growth of callosal neurons.
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- 2021
6. Laboratory Acquired Zika Virus Infection Through Mouse Bite: A Case Report
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Paula Pezzuto, Raissa R. Christoff, Patricia P. Garcez, Filipe R. R. Moreira, Mariane Talon de Menezes, Liane de Jesus Ribeiro, Orlando da Costa Ferreira Júnior, Luiza M. Higa, Amilcar Tanuri, Richard Araújo Maia, and Renato S. Aguiar
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biology ,business.industry ,Transmission (medicine) ,viruses ,030231 tropical medicine ,transmission ,biology.organism_classification ,Virology ,Genome ,Virus ,Zika virus ,03 medical and health sciences ,Flavivirus ,AcademicSubjects/MED00290 ,Zika ,0302 clinical medicine ,Infectious Diseases ,Oncology ,Mouse bite ,mouse bites ,Medicine ,Novel ID Cases ,030212 general & internal medicine ,business - Abstract
Zika virus is an arthropod-borne flavivirus mainly transmitted by the bite of infected mosquitoes. However, alternative transmission routes can occur. In this study, we show the accidental transmission of virus from an infected mouse to a human during the experimental manipulation. This study describes the patient clinical manifestations and virus genome identification., We describe a new route of transmission of Zika virus from an infected mouse to human during experimental manipulation.
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- 2020
7. Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly
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Patricia P. Garcez, Ali Mirazimi, Veronica Krenn, Thomas R Burkard, Arianna Calistri, Jürgen A. Knoblich, Cristiano Salata, Raissa R. Christoff, Julia Spanier, Ulrich Kalinke, Camilla Bosone, Krenn, V, Bosone, C, Burkard, T, Spanier, J, Kalinke, U, Calistri, A, Salata, C, Rilo Christoff, R, Pestana Garcez, P, Mirazimi, A, and Knoblich, J
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Microcephaly ,neural progenitor ,viruses ,neural progenitors ,Herpesvirus 1, Human ,medicine.disease_cause ,brain organoid ,Article ,Virus ,Zika virus ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Pregnancy ,Interferon ,brain organoids, microcephaly, Zika virus, herpes simplex virus, neural progenitors, neuroepithelial polarity, interferons, innate immune response ,Genetics ,medicine ,Organoid ,Humans ,030304 developmental biology ,0303 health sciences ,Innate immune system ,brain organoids ,neuroepithelial polarity ,biology ,Zika Virus Infection ,BIO/13 - BIOLOGIA APPLICATA ,interferon ,Zika Virus ,Cell Biology ,herpes simplex virus ,medicine.disease ,biology.organism_classification ,herpes simplex viru ,Virology ,Zika viru ,interferons ,Organoids ,Herpes simplex virus ,innate immune response ,Molecular Medicine ,Female ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Viral infections in early pregnancy are a major cause of microcephaly. However, how distinct viruses impair human brain development remains poorly understood. Here we use human brain organoids to study the mechanisms underlying microcephaly caused by Zika Virus (ZIKV) and Herpes Simplex Virus (HSV-1). We find that both viruses efficiently replicate in brain organoids and attenuate their growth by causing cell death. However, transcriptional profiling reveals that ZIKV and HSV-1 elicit distinct cellular responses and HSV-1 uniquely impairs neuroepithelial identity. Furthermore, we demonstrate that while both viruses fail to potently induce the type I interferon system, the organoid defects caused by their infection can be rescued by distinct type I interferons. These phenotypes are not seen in 2D cultures, highlighting the superiority of brain organoids in modeling viral infections. Together, these results uncover virus-specific mechanisms and complex cellular immune defenses associated with virus-induced microcephaly.
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- 2021
8. Long-Term Exposure to Paraquat Alters Behavioral Parameters and Dopamine Levels in Adult Zebrafish (Danio Rerio)
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Josiane Woutheres Bortolotto, Luiza Wilges Kist, Maurício Reis Bogo, Raissa R Christoff, Giana de Paula Cognato, Laura N Roesler, Carlos Eduardo Leite, Monica R. M. Vianna, and Carla Denise Bonan
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Fish Proteins ,Paraquat ,medicine.medical_specialty ,Tyrosine 3-Monooxygenase ,Dopamine ,Danio ,Polymerase Chain Reaction ,chemistry.chemical_compound ,Internal medicine ,Gene expression ,medicine ,Animals ,Zebrafish ,Dopamine transporter ,Dopamine Plasma Membrane Transport Proteins ,Behavior, Animal ,biology ,Tyrosine hydroxylase ,Herbicides ,Anatomy ,Zebrafish Proteins ,biology.organism_classification ,Endocrinology ,Gene Expression Regulation ,chemistry ,Systemic administration ,biology.protein ,3,4-Dihydroxyphenylacetic Acid ,Animal Science and Zoology ,Developmental Biology ,medicine.drug - Abstract
Chronic exposure to paraquat (Pq), a toxic herbicide, can result in Parkinsonian symptoms. This study evaluated the effect of the systemic administration of Pq on locomotion, learning and memory, social interaction, tyrosine hydroxylase (TH) expression, dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) levels, and dopamine transporter (DAT) gene expression in zebrafish. Adult zebrafish received an i.p. injection of either 10 mg/kg (Pq10) or 20 mg/kg (Pq20) of Pq every 3 days for a total of six injections. Locomotion and distance traveled decreased at 24 h after each injection in both treatment doses. In addition, both Pq10- and Pq20-treated animals exhibited differential effects on the absolute turn angle. Nonmotor behaviors were also evaluated, and no changes were observed in anxiety-related behaviors or social interactions in Pq-treated zebrafish. However, Pq-treated animals demonstrated impaired acquisition and consolidation of spatial memory in the Y-maze task. Interestingly, dopamine levels increased while DOPAC levels decreased in the zebrafish brain after both treatments. However, DAT expression decreased in the Pq10-treated group, and there was no change in the Pq20-treated group. The amount of TH protein showed no significant difference in the treated group. Our study establishes a new model to study Parkinson-associated symptoms in zebrafish that have been chronically treated with Pq.
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- 2014
9. Y-Maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods
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Josiane Woutheres Bortolotto, Diogo R. Lara, Ana R Blazina, Monica R. M. Vianna, Giana de Paula Cognato, Raissa R Christoff, and Carla Denise Bonan
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Male ,Cognitive Neuroscience ,Cholinergic Agents ,Danio ,Glutamic Acid ,Experimental and Cognitive Psychology ,Task (project management) ,Random Allocation ,Behavioral Neuroscience ,Glutamatergic ,Discrimination, Psychological ,Memory ,Neural Pathways ,Avoidance Learning ,Animals ,Excitatory Amino Acid Agents ,Maze Learning ,Zebrafish ,Behavior, Animal ,biology ,behavior ,Novelty ,Recognition, Psychology ,zebrafish ,biology.organism_classification ,Acetylcholine ,Cholinergic Neurons ,Exploratory Behavior ,Cholinergic ,NMDA receptor ,Memory consolidation ,Psychology ,Neuroscience ,Behavioral Research - Abstract
The interest in the behavioral features of zebrafish has significantly increased over the past two decades. However, most available protocols have used longer training periods and have been based on reinforcement/reward or avoidance. The Y-Maze memory task has the advantage of using a simple and rapid training session, but it has not been established in zebrafish. Here, we have characterized this task for zebrafish, with the addition of pharmacological interventions in the acquisition and consolidation memory phases. The results show that zebrafish spend more time in the novel arm than in the other arms of the Y-Maze, both in response to novelty and spatial memory training-test intervals (TTIs). We have also studied the involvement of the glutamatergic and cholinergic systems with pre- and post-training treatments with the NMDA receptor antagonist MK-801 (20 μM) and the cholinergic blocker scopolamine (200 μM). After 1h of TTI, pre-training MK-801 and scopolamine-treated fish reduced their exploration of the novel arm when compared to the control group, with no changes in their locomotor activity. Post-training of MK-801 treatment also impaired their Y-Maze performance, while post-training of any scopolamine treatment failed to affect novel arm exploration. In conclusion, the Y-Maze memory task can be reliably used for zebrafish, providing a new, rapid, and preference/avoidance independent task for the study of memory in this teleost. In addition, our results highlight the implication of the glutamatergic and cholinergic systems in the memory of zebrafish.
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- 2012
10. Impairment of object recognition memory by rapamycin inhibition of mTOR in the amygdala or hippocampus around the time of learning or reactivation
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Nadja Schröder, Aline Werenicz, Rafael Roesler, Natasha Maurmann, Thiago Rodrigues Pedroso, Raissa R. Christoff, Paulo Fernandes Costa Jobim, and Gustavo Kellermann Reolon
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Male ,Microinjections ,Hippocampus ,Inhibitory postsynaptic potential ,Amygdala ,Behavioral Neuroscience ,medicine ,Animals ,Learning ,Enzyme Inhibitors ,Rats, Wistar ,Novel object recognition ,PI3K/AKT/mTOR pathway ,Recognition memory ,Sirolimus ,TOR Serine-Threonine Kinases ,Retention, Psychology ,Recognition, Psychology ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Rats ,medicine.anatomical_structure ,Mental Recall ,Synaptic plasticity ,Memory consolidation ,Psychology ,Neuroscience - Abstract
The role of the basolateral complex of the amygdala (BLA) in recognition memory remains poorly understood. The mammalian target of rapamycin (mTOR) in the BLA and other brain areas has been implicated in synaptic plasticity and memory. We have recently shown that mTOR signaling in both the BLA and the dorsal hippocampus (DH) is required for formation and reconsolidation of inhibitory avoidance, a fear-motivated memory task. Here we examined the effects of infusions of the mTOR inhibitor rapamycin into the BLA before or after either training or reactivation on retention of novel object recognition (NOR) memory in rats, and compared the effects with those obtained using intra-DH infusions. Male Wistar rats received bilateral infusions of vehicle or rapamycin into the BLA or DH before or after NOR training or reactivation. Rapamycin impaired NOR retention tested 24 h after training when given either before or immediately after training into the BLA or DH. Rapamycin also impaired retention measured 24 h after reactivation when infused before reactivation into the BLA or DH, or immediately after reactivation into the BLA, but not when given 6 h after reactivation into either the BLA or DH. The results suggest that mTOR signaling in the BLA and DH is involved in NOR memory formation and stabilization.
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- 2012
11. Inhibition of mTOR by rapamycin in the amygdala or hippocampus impairs formation and reconsolidation of inhibitory avoidance memory
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Gustavo Kellermann Reolon, Natasha Maurmann, Raissa R. Christoff, Rafael Roesler, Paulo Fernandes Costa Jobim, Thiago Rodrigues Pedroso, and Aline Werenicz
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Male ,Cognitive Neuroscience ,Hippocampus ,Experimental and Cognitive Psychology ,Inhibitory postsynaptic potential ,Amygdala ,Behavioral Neuroscience ,Memory ,Avoidance Learning ,medicine ,Animals ,Memory impairment ,Rapamycin ,Rats, Wistar ,PI3K/AKT/mTOR pathway ,Neurons ,Sirolimus ,Electroshock ,TOR Serine-Threonine Kinases ,Reconsolidation ,Retention, Psychology ,Inhibitory avoidance ,Rats ,medicine.anatomical_structure ,Synaptic plasticity ,mTOR ,Memory consolidation ,Protein synthesis ,Psychology ,Neuroscience ,Consolidation ,Basolateral amygdala - Abstract
Mammalian target of rapamycin (mTOR), a central regulator of protein synthesis in neurons, has been implicated in synaptic plasticity and memory. Here we show that mTOR inhibition by rapamycin in the basolateral amygdala (BLA) or dorsal hippocampus (DH) impairs both formation and reconsolidation of memory for inhibitory avoidance (IA) in rats. Male Wistar rats received bilateral infusions of vehicle or rapamycin into the BLA or DH before or after IA training or retrieval. Memory retention was tested at different time points after drug infusion. Rapamycin impaired long-term IA retention when given before or immediately after training or retrieval into the BLA. When infused into the DH, rapamycin produced memory impairment when given before training or immediately after retrieval. The impairing effects of post-retrieval rapamycin required memory retrieval and were not reversed by a reminder shock. The results provide the first evidence that mTOR in the BLA and DH might play a role in IA memory reconsolidation.
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- 2012
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12. Administration of the phosphodiesterase type 4 inhibitor rolipram into the amygdala at a specific time interval after learning increases recognition memory persistence
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Martina Blank, Paulo Fernandes Costa Jobim, Raissa R. Christoff, Thiago Rodrigues Pedroso, Aline Werenicz, Nadja Schröder, Rafael Roesler, and Gustavo Kellermann Reolon
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Male ,Phosphodiesterase Type 4 ,Time Factors ,Cognitive Neuroscience ,Specific time ,Pharmacology ,Amygdala ,Persistence (computer science) ,Cellular and Molecular Neuroscience ,medicine ,Animals ,Learning ,Rats, Wistar ,Rolipram ,Recognition memory ,Chi-Square Distribution ,Long-term memory ,Retention, Psychology ,Recognition, Psychology ,Rats ,Neuropsychology and Physiological Psychology ,medicine.anatomical_structure ,Exploratory Behavior ,Memory consolidation ,Phosphodiesterase 4 Inhibitors ,Psychology ,Photic Stimulation ,medicine.drug ,Cognitive psychology - Abstract
Here we show that administration of the phosphodiesterase type 4 (PDE4) inhibitor rolipram into the basolateral complex of the amygdala (BLA) at a specific time interval after training enhances memory consolidation and induces memory persistence for novel object recognition (NOR) in rats. Intra-BLA infusion of rolipram immediately, 1.5 h, or 6 h after training had no effect on retention tested at 1, 7, and 14 d later. However, rolipram infused 3 h post-training promoted memory persistence for up to at least 14 d. The findings suggest that PDE4 inhibition in the BLA can enhance long-term memory formation when induced specifically 3 h after learning.
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- 2012
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