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The use of uncrewed systems within NOAA as a research and operational tool is well-documented. The full benefits of uncrewed systems to achieving the NOAA mission have remained largely unrealized, primarily because of the programmatic nature of these deployments. Establishment of enterprise services to coalesce uncrewed systems activities is essential for NOAA to optimize the use and benefits of these systems. A robust and encompassing data enterprise is principal among these needed enterprise services, and is essential to the success of NOAA's uncrewed systems strategy. The NOAA Uncrewed Systems Strategic Implementation Plan presents a comprehensive multi-year plan of action that builds upon the framework established in the NOAA Uncrewed Systems Strategy. This manuscript describes the Uncrewed Systems Data Enterprise as outlined in the Implementation Plan. In Section II we describe the data enterprise milestones. The notional operational framework and planned case studies are described in Sections III and IV, respectively. The advantages of the data enterprise are summarized in Section V.

Purpose: Antibodies that target immune checkpoint molecules have demonstrated significant and durable clinical benefit through re-activation and proliferation of pre-existing tumor-infiltrating CD8+ T cells in a broad range of tumor types including lung cancer. Detailed characterization of T cell dynamics at clonal levels using nextgeneration T cell receptor (TCR) sequencing in large cohorts of lung cancer patients is yet reported.Methods: We performed TCR sequencing of peripheral blood samples (and some tumors) obtained from 27 lung cancer patients undergoing single/combined immune checkpoint blockade therapies.Results: In one responder, we found expansion of a single T cell clone (approximately 20% of the all TCR reads) in a metastatic subcutaneous lesion that showed pathologic complete response on day 17 of treatment. The same TCR CDR3 sequence was detected in 6.1% of TCR reads in the peripheral blood prior to treatment initiation and the expansion remained persistent in peripheral blood at 21.0% at week 10 and 24.3% at week 48. In other patients who showed durable response, we also found persistent oligoclonal T cell expansion in their peripheral blood which was not observed in non-responders even while they remained on therapy.Conclusion: We found sustained expansion of oligoclonal T cell clones in lung cancer patients who had durable response to immune checkpoint blockade. This suggests possible use of longitudinal TCR sequencing to assist clinical decision-making, assess synergism with other agents, and most importantly facilitate rational development of alternative treatment strategies for non-responders.

This Environmental Assessment has been developed for the Replace Family Housing Phase 6 and Phase 7 design and construction project at Malmstrom AFB. The current family housing situation at Malmstrom AFB is very poor. Of the 1,471 housing units on base, only 498 are deemed adequate according to current Air Force Housing Guidance (USAF 1995a, 2003). Many of the existing homes have deteriorated and the following problems have been reported electrical wiring and fixtures that do not meet current building codes, plumbing fixtures that have corroded, outdated flooring, asbestos is present in flooring and countertops, and lead-based paint has been detected on several interior and exterior surfaces. The Air Force proposes to build new housing units within the existing housing area to remedy these needs.

This EA has been developed for the Replace Family Housing Phase V (Jupiter) design and construction project at Malmstrom AFB. The current family housing situation at Malmstrom AFB is very poor. Of the 1,406 housing units on base, only 377 are deemed adequate according to current AF Housing Guidance (USAF 1995a, 2003). Many of the existing homes have deterioirated and the following problems have been reported: electrical wiring and fixtures that do not meet current building codes, plumbing fixtures that have corroded, outdated flooring, asbestos is present in flooring and countertops, and lead-based paint has been detected on several interior and exterior surfaces. The AF proposes to build new housing units within the existing housing area to remedy these needs.

Soft X-ray contact microscopy is a relatively new form of ultrastructural imaging, having better than 6 nm resolution and being uniquely well suited for the examination of fragile, unstained biological specimens. The biological specimen placed on a layer of photoresist and exposed to soft X-rays (1-10 nm lambda) of a specific wavelength or broad band. After X-ray exposure, the specimen is removed from the photoresist and the latter chemically developed. When the developed replica is examined by high resolution scanning electron microscopy, the fine structure of the original biological specimen is faithfully reproduced. Since the soft X-ray replica is initially formed due to the differential absorption of the incident X-rays by the biological specimen, the resultant contact replica also reveals information about the elemental composition of the sample. This paper presents our application of this new technique for the study of the proteoglycans, the complex polyanionic macromolecules comprising the gel phase in the matrix of mammalian cartilage.

During the past 10 years, attempts have been made to examine hydrated biological specimens by using wet chambers (at ambient temperature) [1-3] or cold stages (at -30 degrees C and below) during electron microscopic examination. Obtaining sufficient contrast of unstained hydrated biological samples has proven a considerable problem using both of these methods. Many fragile biological specimens, when examined hydrated, frozen or dried, are severely damaged by the electron beam and cannot be imaged by conventional scanning or transmission electron microscopy. In order to increase specimen contrast and eliminate electron beam induced trauma to the specimen, we have developed a wet-cell [4], which when used in concert with a pulsed plasma soft X-ray source, provides high contrast contact replicas of totally hydrated, unstained biological specimens. Although it has been postulated that hydrated unstained samples can be imaged by soft X-ray contact microscopy [5-7], to date there has been little success due to cell movement or degradation of the wet sample during the long exposure period necessary for an adequate imaging dose [8]. With the pulsed plasma source described in this study we have been able to use exposure times of approximately 40-60 ns while maintaining the sample in its hydrated state at atmospheric pressure. The resultant contact replicas exhibit good contrast and better than 30 nm spatial resolution when examined by conventional scanning electron microscopy.