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Immunological deficiency observed during the transition period in dairy cows promotes development of puerperal diseases. Thus, the research aimed to evaluate hematological (leukogram) and biochemical (glucose, fibrinogen, and total proteins) parameters in Holstein cows (n = 44) before (day -20) and after (day +20) parturition and the possibility of identifying initial signs of reproductive illness, thereby assisting the professional in decision making. There was a difference in the number of total leukocytes, which decreased postpartum (8,888.4 cells/mm3). There was no difference in neutrophils at both time points. Females who presented high concentration of fibrinogen (5.2 g/L) and total plasmatic proteins (78.9 g/L) at postpartum presented retention of fetal appendages and later uterine disease. These results are consistent with the literature on antepartum leukocytosis and inflammation indicated by increased fibrinogen and plasmatic protein and differ in terms of neutrophil behavior. Thus, laboratory assays can be complementary tools to physical examination, aiding in the diagnosis and identification of changes even before the appearance of clinical signs in animals, which is necessary for the prevention of metabolic and infectious disorders.

Colostrum is the main source of immunoglobulins for piglets and several studies have shown that colostrum intake by piglets have a significant influence on their immunity as well as their future performance. The aim of this study was to ascertain the correlation between colostrum intake in the first 24 hours and the serum immunoglobulin concentration determined by immunocrit (IR), determine if the birth order of piglets interfered in colostrum intake and the IR, and measure their weight gain up to 42 days of age. One hundred and three piglets were included in the study and divided into two groups according to colostrum intake in the first 24 hours: > 230 g and 0.05). The mean IR between colostrum intake groups was 0.075 (7 presented with an IR=0.079 (p230 g) in the first 24 hours after birth had a greater immunocrit and greater weight gain from 7 to 42 days of age (p0,05). O IR médio entre os grupos de ingestão de colostro foi de 0,075 (7, apresentaram IR 0,079 (p

Understanding the flow of processes in swine production systems and how they work is fundamental to improving reproductive performance. We surveyed 32 boar studs in Brazil, representing 61.53% of the total in the country. Commercial lines were the most common breed (59.38%) used in the studs. Individual pens and stalls were the most commonly used as housing system (71.9%), and 81.25% of the studs reported having some form of thermal control system. For most of the studs (62.51%), average weekly sperm concentration was higher than 200 million cells per mL. Also, for most studs (71.88%) average weekly ejaculate volume was more than 250 mL. In 46.88% of the studs each ejaculate yielded 26 to 40 semen doses. In 6.25% of the studs, 3.5 billion sperm cells per dose were used for artificial, intracervical insemination. Sperm concentration in 46.88% of the studs was determined using a computer-assisted system analysis. The assessment of sperm quality was conducted using morphology, concentration, and microbiological testing. Employees working on semen collection had formal education of elementary/middle school (34.38%) and high school (37.5%). Most of the laboratory technicians had frequented high school (75%). The most time-consuming task was semen processing, taking 16 to 25 h a week (46.8%) and 6 to 10 h was allotted for the cleaning of stud facilities (46.8%). The data collected in the present study allow greater knowledge of this important part in the pig production chain in Brazil. RESUMO: O entendimento dos processos na suinocultura e como estes funcionam é fundamental para o incremento da performance reprodutiva. Por via eletrônica, foi realizada uma investigação com 32 centrais de coleta e processamento de sêmen no Brasil, representando 61,53% do total no país. O número médio de reprodutores por central foi de 122. Reprodutores da linhagem comercial foram os mais frequentemente (59,38%) utilizados nas centrais. As baias para alojamento individual dos reprodutores foram as mais comuns (71,9%) e 81,25% das centrais relataram possuir algum sistema de climatização. Na maioria das centrais (62,51%), a média da concentração espermática foi maior que 200 milhões de espermatozoides/mL. Da mesma forma, a maioria das centrais (71,88%) apresentaram um volume do ejaculado superior a 250 mL. Em 46,88% das centrais um ejaculado produziu de 26 a 40 doses inseminantes. Em 6,25% das centrais eram utilizadas uma concentração de 3,5 bilhões de espermatozoides por dose inseminante na inseminação intracervical. A concentração espermática em 46,88% das centrais era determinada através do sistema CASA. A avaliação da qualidade espermática era realizada através da análise de morfologia, concentração e exame microbiológico das doses inseminantes. Os colaboradores envolvidos com a coleta de sêmen tinham nível de instrução fundamental (43,38%) e médio (37,5%). A maioria dos técnicos no laboratório das centrais tinham ensino médio (75%). A atividade que mais consumia tempo foi o processamento do sêmen, com 16 a 25 horas/semana (46,8%) e 6 a 10 horas era utilizada na limpeza das instalações (46,8%). Os dados coletados no presente estudo permitem um maior conhecimento desse elo da cadeia produtiva da suinocultura no Brasil.

Gastrointestinal parasites are important, since they can cause a decrese in the performance of swine. The occurrence of parasites in pig farms can create economic losses such as reduction in the value of carcasses and increasing expenses with medicines and veterinary assistance. This study aimed at investigating the occurrence of intestinal parasites in different phases of production reared pigs in intensive prodution systems in the western region of the State of Santa Catarina. A total of 403 fecal samples were analyzed, of which 51.86% were positive for one or more parasites in different phases of production. Finishing pigs had the highest prevalence of parasites (60.19%) followed by nursery pigs (55.44%), pigs in breading sows (50.49%), and suckling piglets (40.81%). Strongylida parasites had higher occurrence (28.78%), and were also the most frequent in the finishing, breading sows, and nursery phases. Coccidia were more occurrent in suckling piglets. This study highlights the need of implementing suitable antiparasitic control measures in all phases of swine production associated with a surveillance system for the diagnosis of endoparasite infection in pigs. Our results demonstrate that endoparasites are highly occurring in pigs, even in intensive system animals on technified farms.

Objective: To assess the microbiological profile in seven Boar Studs (BS) in Southern Brazil, as well as evaluate antimicrobial susceptibility response to most commonly found microorganisms in BS. Material and methods: Bacteriologic analysis was carried out in samples from the water purification system, semen extender, raw and stored semen, lab benches, and other working surfaces. Results: Growth of a mixed bacterial population was observed in water samples from all but one BS. Approximately 85% of the BS had significant contamination on their working surfaces with at least one bacterial contaminant. A total of 86% of raw semen samples were contaminated with one or more different bacteria, while 100% of the boar studs provided contaminated samples. Bacterial susceptibility to antimicrobial agents varied from over 80% for gentamycin, neomycin and ceftiofur to 40% or less for penicillin and lincomycin. Conclusions: The identification of the critical points provides necessary support to devise better strategies to minimize contamination in BS. Also, assessing the level of antimicrobial drug resistance offers accurate information to formulate more efficient antibacterial protocols that closely observe the rational use of antibiotics., Objectivo: Evaluar el perfil microbiológico de siete centros de recogida de semen porcino (CRSP) en el sur de Brasil, así como evaluar la susceptibilidad antimicrobiana a la mayoría de los microorganismos encontrados en los CRSP. Material y métodos: El análisis bacteriológico se realizó en muestras del sistema de purificación de agua, diluyentes de semen, semen crudo y almacenado, bancos de laboratorio y otras superficies de trabajo. Resultados: El crecimiento de una población bacteriana mixta se observó en muestras de agua de todas las CRSP excepto una. Aproximadamente el 85% de la CRSP tenía contaminación significativa en sus superficies de trabajo con al menos un contaminante bacteriano. Un total de 86% de muestras de semen crudo fueron contaminadas con una o más bacterias diferentes, mientras que el 100% de CRSP proporcionaron muestras contaminadas. La susceptibilidad bacteriana a agentes antimicrobianos varió en más del 80% para gentamicina, neomicina y ceftiofur a 40% o menos para penicilina y lincomicina. Conclusiones: La identificación de los puntos críticos proporciona el apoyo necesario para idear mejores estrategias para minimizar la contaminación en CRSP. Además, la evaluación del nivel de resistencia a los antimicrobianos ofrece información precisa para formular protocolos antibacterianos más eficientes que tengan en cuenta el uso racional de los antibióticos.

Modern swine production employs a high degree of technology and is organized in various production stages, in which reproduction is one of the most significant. However, reproductive losses associated to fetal death are still high. Fetal losses, including fetal mummification, may occur at a rate below 1.5% in a sound herd. The causes of fetal death can be very diverse, and include various infectious agents. Therefore, investigating the causes of fetal losses is important in the decision-making process related to the control and prophylaxis of the herd. The goal of this study was to carry out a molecular survey, analyzing porcine circovirus 2 (PCV2), porcine parvovirus (PPV), and pathogenic Leptospira as potential agents related to fetal death in swine farms in Southern Brazil. We collected samples from three farms with a mummified index ≥ 2.5%. Fragments of brain, lung, kidney, liver, and heart were sampled for PCR analysis. Out of 100 samples examined, 87, 68, and 22 were positive for PCV2, PPV, and Leptospira spp., respectively. Moreover, we also identified coinfections with two and three pathogens in the same sample. Our findings contribute to the spreading of scientific knowledge related to infectious causes (PPV, PCV2, and pathogenic leptospires) of fetal losses in swine, as evidenced by the high frequencies of the investigated agents. RESUMO: A produção moderna de suínos emprega um alto grau de tecnologia e é organizada em várias etapas de produção, nas quais a reprodução é uma das mais importantes. No entanto, as perdas reprodutivas associadas à morte fetal ainda são altas. Perdas fetais, incluindo mumificação, podem ocorrer em taxa abaixo de 1,5% em rebanhos sadios. As causas da morte fetal podem ser diversas, incluindo causas infecciosas. Portanto, a investigação de causas relacionadas à ocorrência de perdas fetais é importante no processo relacionado ao controle e profilaxia do rebanho. O objetivo deste estudo foi realizar um levantamento molecular, incluindo circovírus suíno 2 (PCV2), parvovírus suíno (PPV), e leptospiras patogênicas como potenciais agentes relacionados à morte fetal em suínos no sul do Brasil. Foram coletadas amostras de três granjas com índice de fetos mumificados ≥ 2,5%. Fragmentos de cérebro, pulmão, rim, fígado e coração foram amostrados para análise por PCR. Das 100 amostras examinadas, 87 foram positivas para PCV2, 68 foram positivas para PPV e 22 foram positivas para Leptospira spp. Além disso, também identificamos co-infecções com dois e três patógenos em uma mesma amostra. Os resultados encontrados contribuem para a disseminação do conhecimento científico relacionado às causas infecciosas (PCV2, PPV e Leptospiras patogênicas) relacionadas às perdas fetais em suínos, assim como evidenciam a alta frequência dos agentes pesquisados.

The goal of this study was to assess the efficacy of the re-utilization of an ear implant impregnated with norgestomet on estrus synchronization response and pregnancy rates in sheep. Fifty-five Texel ewes were classified according to body condition (3.5) and live weight (65 kg), and randomly assigned to two experimental groups: NORN-new (n = 30) and NORU-used (n = 25). The dose of norgestomet used in the treatments was half of that recommended for bovine (1.5 mg). The synchronization protocol consisted of an ear implant inserted on day 0 (beginning of the experiment) and kept for 6 days. The removal of the implant on day 6 was followed by an injection of a prostaglandin analogue (0.263 mg) and eCG (250 IU). Rams with paint applied to their chest were used to facilitate estrus detection for 5 days following implant withdrawal. Estrus behavior was observed in 93.3% (28/30) and 90% for NORN and NORU, respectively. Pregnancy rates for NORN were 73.3% and for NORU were 68%. Estrus behavior and pregnancy rates between treatment groups did not differ statistically. Therefore, the re-utilization of ear implants impregnated with norgestomet, in addition to eCG and a prostaglandin analogue in short-term estrus synchronization protocols, allow acceptable estrus response and pregnancy rates in sheep.

The clinical implications of recently discovered porcine circovirus 3 (PCV3) infections are still unknown. The potential role of this emerging virus in reproductive loss in swine has been described. Herein, we report a high prevalence of PCV3 in mummified fetuses from sows maintained in modern farms in Rio Grande do Sul, Santa Catarina, Paraná, Goiás, and Mato Grosso do Sul states, Brazil. For this analysis, 276 mummified fetuses from 11 commercial swine farms were included. The presence of PCV3 DNA was confirmed using PCR, and the complete sequence of five different viral strains was obtained. Sequences of PCV3 genomes available on GenBank were then used for phylogenetic tree construction. Of the 276 mummified fetuses examined, 270 (nearly 97%) were positive for PCV3. In 93.1% of the fetuses, co-infections with at least one of the following agents were identified: porcine parvovirus (PPV), porcine circovirus 2 (PCV2) and Leptospira spp. Twelve fetuses were positive for PCV3 alone. The amino acid sequence of the capsid gene for the five viral strains shared 98-100% homology among them. Analysis of the DNA sequence indicates that the viruses identified in this study belong to the PCV3a1 subgroup. In summary, PCV3 DNA was detected in mummified fetuses at a surprisingly high rate. The role of PCV3 in porcine circovirus-associated disease (PCVAD) is still uncertain. However, considering that PCV3 has been detected in a variety of conditions, even in healthy animals, the present results confirm the need to investigate PCV3 as a causative agent of fetal mummification in swine.

Esta pesquisa foi realizada com o objetivo de avaliar o efeito da inclusao de cloreto de amonio na dieta pre-parto de ovelhas leiteiras sobre o pH urinario, calcio serico e metabolismo energetico dos animais. Foram utilizadas vinte femeas ovinas da raca Lacaune, separadas em dois grupos: o Grupo Controle (GC, n=10) que recebeu a dieta pre-parto sem adicao de cloreto de amonio e o Grupo Tratado (GT, n=10) que recebeu a dieta pre-parto com a inclusao de 0,4% de cloreto de amonio do total de materia seca (MS) consumida por animal/dia. As coletas de urina e sangue ocorreram aos 120 (M0), 127 (M1) e 135 (M2) dias de gestacao. Foram avaliados o pH urinario e os niveis sericos de calcio, cetonas, glicose e colesterol. Houve reducao no pH urinario no GT nos dias 127 e 135 de gestacao. Os valores de cetonas sericas foram inferiores no GT no dia 135 de gestacao enquanto que os niveis de calcio e colesterol foram superiores neste grupo no mesmo momento tempo avaliado. Nao houve diferenca nos valores de glicose entre os grupos. Desta forma, conclui-se que a inclusao de 0,4% de cloreto de amonio do total de materia seca consumida por animal/dia na dieta pre-parto de ovelhas leiteiras foi eficiente na manutencao da calcemia neste periodo e que esta pode estar associada a nao reducao do status energetico em ovelhas gestantes.

Na inseminacao artificial de suinos, sao utilizados de 9 a 12 bilhoes de espermatozoides por femea inseminada. A utilizacao de menor numero de espermatozoides, sem interferir na performance reprodutiva das femeas, poderia otimizar o uso dos machos e reduzir os custos de inseminacao. Esta tese foi dividida em tres experimentos para avaliar o efeito da reducao do numero de espermatozoides por femea inseminada com a tecnica tradicional ou intra-uterina. No experimento 1 foram utilizadas 218 leitoas Camborough 22 ® inseminadas em tres intervalos antes da ovulacao (0-12, 13-23 e 24-30 h), com doses inseminantes (DIs) contendo 1,5 bilhao de espermatozoides e armazenadas por 0-48 h ou 96-120 h. As leitoas receberam uma unica inseminacao. As femeas foram abatidas aos 30,8±3,7 dias de gestacao para contagem do numero de corpos luteos (CL) e embrioes totais (ET). A taxa de prenhez (TPr) foi influenciada pelo intervalo inseminacao-ovulacao (IAOV) quando o semen foi armazenado por 96-120 h (P ® . As femeas foram distribuidas em quatro tratamentos (doses de 1 ou 2 bilhoes de espermatozoides diluidos em 60 ml e intervalo IAOV de 0-24 e 25-36 h). As femeas receberam uma unica IAU. No momento da inseminacao, nao foi observado refluxo de semen. A passagem do cateter pela cervix foi possivel em todas as femeas. Foi observada presenca de sangue em 1,7% das femeas. Aos 31±4,3 dias de gestacao, as femeas foram abatidas para contagem dos CL e ET. A TPr e a SE nao foram afetadas pelo numero de espermatozoides ou pelo intervalo IAOV. O ET nao foi influenciado pelo numero de espermatozoides, mas foi reduzido para o intervalo IAOV de 25-36 h comparado a 0-24h (P ® com o objetivo de comparar o desempenho reprodutivo de femeas submetidas a IAU e a inseminacao tradicional. As femeas foram distribuidas em dois tratamentos: T1: IAU com DIs contendo 0,5 bilhao de espermatozoides em um volume total de 20 ml; T2: inseminacao tradicional com DIs contendo 3,0 bilhoes de espermatozoides em um volume total de 90 ml. As femeas receberam multiplas inseminacoes. Foi possivel a realizacao da IAU em 98,1% das femeas. A presenca de sangue, na extremidade do cateter ou espiral da pipeta de IAU, foi observada em 8,4 % das femeas. A TPr e taxa de parto ajustada nao diferiram entre os tratamentos. No entanto, o tamanho da leitegada foi menor (P

O objetivo deste trabalho foi avaliar os níveis de colesterol e beta-hidroxibutirato sérico em vacas leiteiras com hipocalcemia subclínica. Para isso foram utilizadas 14 vacas leiteiras da raça Holandesa no período de transição. O experimento foi dividido em duas etapas, onde na primeira os animais foram acompanhados quanto ao pH urinário no pré-parto e os níveis de cálcio sérico nos três primeiros dias pós-parto. Na segunda etapa, os animais foram divididos em dois grupos a partir dos níveis de cálcio sérico até as 24 horas pós-parto, sendo: G1: (n=6) animais com cálcio sérico acima de 8 mg/dL e G2 (n=8) animais com cálcio sérico inferior a 8 mg/dL. Foram avaliados os níveis de colesterol sérico no segundo e décimo dias pós-parto e os níveis de beta-hidroxibutirato sérico no décimo dia pós-parto. Observou-se uma redução nos valores de colesterol sérico tanto no segundo como no décimo dia pós-parto e um aumento nos níveis de beta-hidroxibutirato sérico no G2 quando comparado ao G1. Assim, pode-se concluir que a hipocalcemia subclínica afeta negativamente o metabolismo energético, isto demonstrado pela redução dos valores de colesterol sérico e elevação dos níveis séricos de beta-hidroxibutirato.

The objectives of this study were to determine the optimal time of insemination in the pre-ovulatory period (from 32 to 0 h before ovulation) and to evaluate once-daily versus twice-daily inseminations in gilts. In Experiment 1, pre-puberal gilts (n=102) were observed for estrus every 8h and ultrasonography was performed every 8h from the onset of estrus to confirmation of ovulation. The gilts were inseminated once with 4 x 10(9) spermatozoa at various intervals prior to ovulation. Pregnancy detection was conducted 24 days after AI and gilts were slaughtered 4-6 days later. Corpora lutea and the number of viable embryos were counted and the embryo recovery rate was calculated (based on the percentage of corpora lutea). Inseminations performed24h before ovulation resulted in a higher embryo recovery rate (P=0.02) and produced 2.1 more embryos (P=0.01) than inseminationsor=24h before ovulation. However, the pregnancy rate was reduced when inseminations were performed16 h before ovulation (P=0.08). In Experiment 2, pre-puberal gilts (n=105) were observed for estrus every 12h and ultrasonography was performed every 12h from the onset of estrus to confirmation of ovulation. Gilts were inseminated (with 4 x 10(9) spermatozoa) 12h after the onset of estrus, with inseminations repeated either every 12h (twice-daily) or 24h (once-daily) during estrus. The gilts were allowed to farrow. There were no differences (between gilts bred twice-daily versus once-daily) for return to estrus rate (P=0.36) and adjusted farrowing rate (P=0.19). However, gilts inseminated once-daily had 1.2 piglets less than those inseminated twice-daily (P=0.09). In conclusion, gilts should be inseminated up to 16 h before ovulation, as intervals16 h reduced pregnancy rate and litter size.

Foram utilizadas 298 fêmeas pluríparas Camborough 22® distribuídas em dois tratamentos: T1 (n=154), inseminação intra-uterina (IAU) com dose inseminante (DI) contendo 0,5 bilhão de espermatozóides em volume total de 20ml; e T2 (n=144), inseminação tradicional (IAT), com DI contendo 3,0 bilhões de espermatozóides em volume total de 90ml. Foi possível a realização da IAU em 98,1% das fêmeas. A presença de sangue na extremidade do cateter ou espiral da pipeta de IAU foi observada em 8,4% das fêmeas. As taxas de prenhez (TPr) e de parto ajustada não diferiram (P>0,05) entre a IAU e IAT. O tamanho da leitegada (TL) diferiu entre os tratamentos (P0.05) among treatments. Litter size (LS) differ among treatments (P

This study evaluated the reproductive performance of gilts inseminated at three intervals before ovulation (0-12, 13-23, 24-30 h) with sperm doses (SD) stored for 0-48 and 96-120 h. A total of 218 PIC Camborough 22 gilts were inseminated once with SD of 1.5 x 10(9) sperms. Pregnant gilts (n = 166) were slaughtered 30.8 +/- 3.7 days after artificial insemination. The number of corpora lutea (CL) and total embryos (TE) was counted. Pregnancy rates (PR) were analysed by chi-square test. TE and embryonic survival (ES), obtained as the ratio between viable embryos and CL, were analysed by GLM procedure (SAS) and mean values were compared by Tukey's test. Pregnancy rate was similar among artificial insemination-ovulation (AIOV) intervals when semen was stored for 0-48 h. However, the lowest PR was observed in the 24-30 h AIOV interval with storage time (ST) of 96-120 h (p0.05). There was a significant effect of the interaction between ST and AIOV (p0.05) on TE and ES variables. Total embryos and ES did not differ (p0.05) among AIOV intervals in ST of 0-48 h. However, gilts inseminated at 24-30 h AIOV interval with ST of 96-120 h showed a reduction of 6.7 embryos (p0.05) compared with gilts in the same interval inseminated with semen stored for 0-48 h. ES for the 24-30 h AIOV interval and ST of 96-120 h was lower than that observed in the other groups (p0.05).

O objetivo deste estudo foi avaliar o efeito da hipertermia no dia da inseminação artificial (IA) até o 13o dia de gestação, sobre o desempenho reprodutivo de leitoas e porcas. No experimento 1, as 488 fêmeas foram divididas em dois grupos de acordo com a temperatura retal no dia da primeira inseminação artificial (IA), respectivamente, G1 = 39,50C (normotermia) e G2 = >39,50C (hipertermia). As fêmeas com hipertermia apresentaram taxas de retorno ao estro (TRE) maiores e taxas de parto (TP) e tamanho de leitegada (TL) menores (p 39.5oC (hiperthermia). The females having hyperthermia showed higher return to estrus rate (ERR), and lower farrowing rate (FR) and litter size (LS) (p

Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou da E. coli sobre o percentual de NAR e pH nas 96 horas. Quando comparado ao controle, somente a inoculação de 5 x 10(7) UFC/ml de E. coli diferiu, às 96 horas, em relação ao percentual de NAR (p0.05). Also, there was no significant effect (p>0.05) of the S. aureus or E. coli on the percent of NAR and the pH during the 96 hours. When compared to the control group, only the inoculation of 5 x 10(7) CFU/ml of E. coli differed at the 96 hours in relation to the percentage of NAR (p

Condenações de suínos no momento do abate devido a lesões tuberculóides causadas por micobactérias são descritas em diversos países, inclusive no Brasil. No presente trabalho, 146 animais de idades variando entre 2,5 e > 14 meses, provenientes de uma propriedade com problema de condenações por tuberculose foram abatidos e os linfonodos examinados quanto ao tipo de lesão macro e microscópica e à presença de micobactérias. Ao mesmo tempo, a ração, a água e a maravalha utilizadas na propriedade foram investigadas quanto à presença de micobactérias. Macroscopicamente, os linfonodos afetados apresentavam nódulos arredondados de 1-2 mm de diâmetro, de coloração amarelo-clara com conteúdo pastoso a caseoso, delimitados por uma cápsula. Em linfonodos de 12 animais foi possível visualizar bacilos álcool-ácido resistentes, sendo que em 4 deles foi isolado o Mycobacterium-grupo III (Runyon). As lesões foram encontradas principalmente em animais entre 6 e 14 meses de idade. A mesma bactéria foi isolada da maravalha, que pode ter sido a fonte de infecção dos animais. Macroscopic lesions suggestive of tuberculosis in swine lymph nodes caused by mycobacteria were already described in many countries, including Brazil. This survey was performed in a farm with previous diagnostic of tuberculosis: lymph nodes were obtained from 146 pigs, aged between 2.5 and > 14 months, and after macroscopic and microscopic examination they were cultured. Furthermore feed, water and sawdust were cultured. Macroscopic lesions were characterized as nodes with diameter between 1-2 mm, with a pale yellow, loose, caseous content. Lymph nodes from 12 pigs were positive for acid-fast bacteria. Mycobacterium-group III (Runyon) was isolated in four cases. The lesions occurred mainly in pigs aged between 6 and 7 months old. The same mycobacteria were isolated from sawdust. It is concluded that sawdust might be the source of infection.