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Ultrasonic-assisted synthesis of ZnO/TiO₂ and Fe₃O₄/TiO₂ bi-oxide nanocomposites at low frequencies (60 kHz) with their characterizations and antibacterial/antifungal applications are investigated in this study. Using nanotechnology as the next-generation solution, this research explored the potential of metal oxide nanoparticles as effective antibacterial agents. Nanomaterials were synthesized via a novel and facile method (sono-synthesis) and characterized using various techniques (XRD, SEM, FTIR, zeta potential analysis, Raman spectroscopy, and XPS), revealing nanoscale sizes of 25 nm for ZnO/TiO₂ and 31 nm for Fe₃O₄/TiO₂. Structural analysis demonstrated the distinct crystalline phases of the synthesized nanomaterials: TiO₂ with anatase structure (25 %), hexagonal wurtzite structure for ZnO, and Fe₃O₄ with inverse spinel structure, formed in nano-dimensions (19–31 nm) with a near-spherical shape. Antibacterial and antifungal assays highlighted the efficacy of both bi-nanocomposites against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, along with their potent activity against Candida albicans and non-albicans Candida. Moreover, principal component analysis (PCA) was used to identify potential relationships between the physicochemical properties (zeta potential, size, conductivity, and concentration) of all nanomaterials tested and their zone of inhibition (ZOI) against all bacterial species tested. Overall, this study emphasizes the novelty of employing a simple and efficient sono-synthetic route to synthesize ZnO/TiO₂ and Fe₃O₄/TiO₂ bi-nanocomposites and investigate their significant antibacterial and antifungal activities. Additionally, it identifies the potential relationships between the physicochemical properties of the nanomaterials tested and their antimicrobial activity. This approach provides a robust framework for future applications of bi-nanocomposites in combating microbial infections. • Effectively synthesized Fe₃O₄, ZnO, Fe₃O₄/TiO₂, and ZnO–TiO₂ bi-nanocomposites using an ultrasonic process via one-pot synthesis, while also uncovering their inherent antimicrobial potential. • The sono-synthesized nanomaterials were characterized and confirmed by various spectroscopic and microscopic techniques. • XRD confirmed the successful synthesis of bi-nanocomposites and showed the average crystallite size of Fe₃O₄/TiO₂ as 15.79 nm and ZnO/TiO₂ as 10.35 nm. • Particularly, Fe₃O₄/TiO₂ and Fe₃O₄ showed significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. • Both Fe₃O₄/TiO₂ and ZnO/TiO₂ bi-nanocomposites exhibited remarkable antibacterial activity against E. coli and antifungal activity against non-albicans Candida, suggesting their effectiveness in combating fungal pathogens. [ABSTRACT FROM AUTHOR]

This study investigates the degradation of six different types of absorbable surgical threads commonly used in clinical practice, focusing on their response to exposure to physiological fluids. The threads were subjected to hydrolytic and enzymatic degradation in physiological saline, bile, and pancreatic juice. Our findings demonstrate that bile and pancreatic juice, particularly when contaminated with bacterial strains such as Escherichia coli, Klebsiella spp., and Enterococcus faecalis, significantly accelerate the degradation process. Using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and tensile strength testing, we observed distinct differences in the chemical structure and mechanical integrity of the sutures. Principal component analysis (PCA) of the FTIR spectra revealed that PDS threads exhibited the highest resistance to degradation, maintaining their mechanical properties for a longer duration compared with Monocryl and Vicryl. These results highlight the critical role of thread selection in gastrointestinal surgeries, where prolonged exposure to bile and pancreatic juice can compromise the suture integrity and lead to postoperative complications. The insights gained from this study will contribute to improving the selection and application of absorbable threads in clinical settings. [ABSTRACT FROM AUTHOR]

Stilbocrea banihashemiana is a recently described species of ascomycete fungi (Bionectriaceae) that causes canker and dieback diseases on fig and barberry, and dieback on loquat trees. Previous studies suggested this pathogen might affect other trees including economically important fruit crops, such as almonds, apples, and olives. This study assessed the susceptibility of 40 tree species belonging to 20 plant families, including fruit-bearing, ornamental, and edible landscape plants, to S. banihashemiana. The assessment was conducted by wound-inoculating detached shoots and one-year-old saplings with the pathogen. Based on five parameters of induced lesions, we clustered the inoculated tree species using the principal component analysis approach. The results of the approach revealed four distinct groups of tree species based on their susceptibility traits. Almond, apricot, eucalyptus, poplar, and red mulberry clustered in one group as susceptible hosts. The majority of the species examined, which included both tropical and temperate trees, were categorized as moderately susceptible. Platanus and sweet orange were identified as non-host-resistant species, while Arizona cypress, garden privet, and olive were classified as low susceptible. Moreover, our findings suggest a correlation between temperature and the aggressiveness of S. banihashemiana. This study revealed that the emerging pathogen, S. banihashemiana, could have a broader host range than currently recognized. The accidental introduction of this pathogen through the global trade of live host plants may pose a significant threat to economically important trees in temperate and subtropical regions. [ABSTRACT FROM AUTHOR]

The present research investigated the physicochemical properties, phenolic profile, total phenolic content (TPC), total anthocyanin content (TAC), and antioxidant properties of cherry laurel (Prunus laurocerasus L.) pulp and marmalade (with sucrose and stevia addition) during storage. Pulp and marmalades were stored at room temperature and in the refrigerator and analyzed periodically. It was found that sample type, storage temperature, and time had a statistically significant effect on color parameters (L*, a*, b*, C*, and H°). Sample type also significantly affected pH, titratable acidity, total dry matter, ash, reducing sugar, sucrose, total sugar, TPC, TAC, and DPPH antioxidant activity. Storage time affected total dry matter, soluble solids content (SSC), reducing sugar, total sugar, TPC, TAC, and antioxidant activity, whereas storage temperature affected total dry matter, water-soluble dry matter, reducing sugar, total sugar, TPC, TAC, and antioxidant activity. Antioxidant activity, TPC, and TAC decreased with increasing storage time. Samples stored in the refrigerator displayed higher antioxidant activity, TPC, and TAC than those stored at room temperature. Pulp and marmalades contained chlorogenic acid and keracyanin chloride. Principal component analysis (PCA) was conducted to evaluate the impacts of sweetener type, storage time, and temperature. It was determined that sucrose-added marmalade formed a similar group, while pulp and stevia-added marmalade constituted a different group. Finally, this study supported the use of cherry laurel pulp and marmalade as natural sources of antioxidants. [ABSTRACT FROM AUTHOR]

The present study examined the impact of dielectric barrier discharge cold plasma treatment on the physicochemical characteristics of taro peel starch (TPS) and its films. This work reports for the first time on using native and modified TPS in the casting procedure to develop packaging films. The results showed that, as plasma exposure voltage and treatment time prolonged, the pH, 955 cm−1/1022 cm−1, % crystallinity, and turbidity of TPS significantly decreased, while amylose content, solubility, and 1047 cm−1/1022 cm−1 of TPS increased considerably. Despite this, modified TPS‐based films have comparatively smooth and homogeneous surfaces. Moreover, the modified starch‐based film exhibited the lowest water vapor permeability, elongation at break, and solubility, while highest opacity and tensile strength. [ABSTRACT FROM AUTHOR]

The present study examines the water quality in the Quaternary Mio-Plio-Quaternary aquifer of the mining basin of Gafsa using a hydrochemical approach and multivariate statistical methods, to assess groundwater mineralization processes. Results from the analysis of groundwater quality collected during the winter (January 2020) and summer (June 2021) seasons reveal a pronounced stability in geochemical parameters, emphasizing a noteworthy consistency in water composition between the two seasons, with the dominance of the Na-Ca-Mg-SO4-Cl facies, in addition to the fact that all year round these concentrations are beyond their respective WHO limits. Despite the intensive extractive and transformation phosphate industry, the prolonged interaction of water with geological formations is the primary factor controlling their high mineralization. This results from the dissolution of carbonates (calcite, dolomite), gypsum, and halite. The results of the PCA represent two correlation classes. Class 1 comprises major elements sulfate, chloride, sodium, magnesium, and calcium strongly correlated with electrical conductivity (EC) and total dissolved solids (TDS). This correlation is indicative of the water mineralization process. Class 2 includes major elements nitrate and potassium weakly correlated with (TDS) and (EC) As regards heavy metals, their concentrations fall consistently below their respective potability standards established by the WHO across all water sampling points. Meanwhile, fluoride (F-) concentrations exhibited values ranging from (1.6 mg·L−1 to 2.9 mg·L−1) in the winter of January 2020 and (1 to 2.9 mg·L−1) in the summer of June 2021, surpassing its WHO limit (1.5 mg·L−1) in almost all water samples. These findings allow us to conclude that the high mineralization of these waters is acquired due to the dissolution of carbonates (calcite, dolomite), gypsum, and halite due to their prolonged interaction with the geological formations. The deterioration of groundwater quality in the Gafsa mining basin associated with phosphate extraction and processing activities appears to be primarily due to the intensive exploitation of deep-water resources. [ABSTRACT FROM AUTHOR]

Wheat is a vital crop globally, essential for agriculture, economics, and food security. However, in arid and semi-arid conditions, wheat production faces significant challenges due to low water availability, uneven rainfall distribution, and high soil salinity. The germination and early seedling stages are particularly vulnerable to these stresses. Therefore, this study assessed 15 wheat genotypes for their tolerance to salinity stress during early growth stages, using a hydroponic system with four salt stress levels (0, 50, 100, and 150 mM NaCl). Significant differences were observed for genotype and salinity main effects and their interaction on all investigated traits, indicating considerable variability in the response to salt stress among the investigated wheat cultivars. High NaCl concentrations led to substantial reductions in measured parameters across genotypes, with some showing resilience while others exhibited heightened sensitivity. Stress tolerance indices, such as mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress tolerance index (STI) and yield index (YI), were identified as reliable indicators for selecting salt-tolerant wheat cultivars. Consequently, Sidi Okba (G11), Ziad (G12), Tamezghida (G13) and Zidane (G14) emerged as the most promising, displaying acceptable performance under both non-stress and salt-stress conditions. These genotypes could serve as valuable genetic resources for breeding programs aimed at enhancing wheat's salinity tolerance, particularly in arid and semi-arid regions. [ABSTRACT FROM AUTHOR]

In this study, a modern principal component analysis (PCA) of the chemical properties of lunar soils was conducted. American and Soviet results acquired during the Apollo and Luna missions, respectively, were analyzed and compared. The chemical composition of the lunar soil was the focus of our analysis, the main aim of which was to assess any possible differences between the results provided by the missions in question. The results were visualized in two- and three-dimensional spaces. The use of PCA virtual variables enabled the chemical composition of the lunar soil to be fully visualized—something impossible to achieve using traditional techniques—and key similarities and differences among the properties of the lunar soil samples were determined. The sources of any differences were then conceptualized. The work reported in this paper offers new directions for future studies, especially research into the design of new lunar soil simulants for lunar construction and civil engineering programs. [ABSTRACT FROM AUTHOR]

Tillandsia species are plants from the Bromeliaceae family which display biomonitoring capacities in both active and passive modes. The bioaccumulation potential of Tillandsia aeranthos (Loisiel.) Desf. and Tillandsia bergeri Mez acclimated to Southern/Mediterranean Europe has never been studied. More generally, few studies have detailed the maximum accumulation potential of Tillandsia leaves through controlled experiments. The aim of this study is to evaluate the maximum accumulation values of seven metals (Co, Cu, Mn, Ni, Pb, Pt, and Zn) in T. aeranthos and T. bergeri leaves. Plants were immersed in different mono elemental metallic solutions of Co (II), Cu (II), Mn (II), Ni (II), Pb (II), Pt (IV), and Zn (II) ions at different concentrations. In addition, cocktail solutions of these seven metals at different concentrations were prepared to study the main differences and the potential selectivity between metals. After exposure, the content of these metals in the leaves were measured by inductively coupled plasma-optical emission spectrometry. Data sets were evaluated by a fitted regression hyperbola model and principal component analysis, maximum metal loading capacity, and thermodynamic affinity constant were determined. The results showed important differences between the two species, with T. bergeri demonstrating higher capacity and affinity for metals than T. aeranthos. Furthermore, between the seven metals, Pb and Ni showed higher enrichment factors (EF). T. bergeri might be a better bioaccumulator than T. aeranthos with marked selectivity for Pb and Ni, metals of concern in air quality biomonitoring. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Plant Production is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The utilization of heterosis is of great significance in improving rice yield. To explore the physiological and genetic basis for high yield in indica–japonica hybrid rice, Zheyou18 (z18) and Yongyou12 (y12) were used as materials and compared with indica hybrid rice, Zheyou12 (z12); japonica hybrid rice, Liangyoupeijiu (LYPJ); and the conventional lines zhe04B (04B) and zhehui818 (h818) under seedling growth vigor, functional leaf morphology, chlorophyll content, yield component, panicle trait, and InDel heterosis analysis. Z18 and y12 showed the largest increase in plant height 6 d and 9 d after germination; the root dry weight of z18 was 31.2% and 42.0% higher than its parents on the 12th d. The length of functional leaves ranked in the middle, while the width was the largest, resulting in z18 and y12 having the largest leaf area. Yield components showed that z18 and y12 had the highest number of primary branches, spikelets, and grains, and grain yield, which was 58.1 g in z18, increased by 29.8% and 8.7%, respectively, in comparison with h818 and LYPJ. The InDel genetic distance was significantly positively correlated with single spike weight, with r reaching 0.771, making it the only consistent and most correlated among the seven traits. Therefore, we speculated that as the InDel genetic distance expands, heterosis mainly manifests in the increase in single spike weight. This study comprehensively explored the physiological mechanism of yield improvement in indica–japonica-hybrid rice and used InDel genetic distances to study the genetic basis of heterosis, which will be helpful for future rice yield improvement. [ABSTRACT FROM AUTHOR]

Volatile components are one key factor in sample identification, differential analysis, quality control and origin traceability. In order to identify and analyze the differences in volatile substances in different alfalfa seeds, this study used gas chromatography–ion mobility spectrometry (GC-IMS), combined with the Gallery Plot plug-in, and PCA, PLS-DA and other analysis methods. In this way, a comprehensive analysis of volatile components in 10 alfalfa seeds, including aerospace varieties, was conducted. A comparative analysis of the characteristics of different sample compounds using topographic maps and fingerprints led to isolation of 48 kinds of 54 volatile compounds. Among them, esters (9 types), olefins (8 types), ketones (8 types), alcohols (6 types) and aldehydes (6 types) were found to be the most abundant volatile compounds in alfalfa seeds. At the same time, PCA and PLS-DA analysis models showed that esters, ketones and alcohols were the main volatiles causing the differences among alfalfa seeds. Among them, the content of various substances in the ZT2 and ZT3 aerospace varieties were higher than that of other varieties, while the types and contents of volatiles in ZT1, ZM2 and GN3 were relatively low. Therefore, in combination with the differences in maturity of each sample, the 10 varieties of alfalfa were finally divided into three categories, and the varieties of the same series were basically classified into one category. This provides a basis and convenience for future seed screening, identification, traceability and forage breeding. [ABSTRACT FROM AUTHOR]

This study investigates human health risks associated with heavy metals (HMs) occurrence in municipal solid waste (MSW) landfills. For testing of selected MSW landfills steps were involved, including site characterization, soil sampling and chemical testing, statistical analysis, as well as health risk assessment, carcinogenic and non-carcinogenic effects. For the Polish landfill (Radiowo) the average HMs concentrations were found in the following order: Zn (52.74 mg/kg DM) > Pb (28.32 mg/kg DM) > Cu (12.14 mg/kg DM) > Ni (4.50 mg/kg DM) > Cd (3.49 mg/kg DM), while for the Czech landfill (Zdounky): Zn (32.05 mg/kg DM) > Cu (14.73 mg/kg DM) > Ni (4.73 mg/kg DM) > Pb (0.10 mg/kg DM) = Cd (0.10 mg/kg DM). Strong positive correlations between selected HMs demonstrated identical origins. Principal component analysis (PCA) performed for the Radiowo landfill transferred the soil parameters into three principal components (PCs), accounting for 87.12% of the total variance. The results of the PCA analysis for the Zdounky landfill revealed three PCs responsible for 95.16% of the total variance. The exposure pathways of HMs for landfills were in the following order: ingestion > dermal absorption > inhalation. For both landfills, the values of hazard quotient were lower than 1, indicating no potential negative health effects. In terms of the hazard index (HI), for both landfills, no adverse human health effects occur (HI < 1). The incremental lifetime cancer risk (ILCR) values indicated negligible or acceptable carcinogenic risk of HMs (average ILCR in the range from 5.01E−10 to 5.19E−06). [ABSTRACT FROM AUTHOR]

Abstract Background Capparis spinosa L. is a typical desert plant that is resistant to high temperatures and drought, and at the same time is rich in medicinal and food values. The objective of this study is to explore the variations in nutrient composition, morphological characteristics, and SDS-PAGE patterns of caper seeds from different provenances, aiming to provide insights for the selection of superior seed provenances. Results In this experiment, there were significant differences in the morphological characteristics and major nutritional components of caper seeds from different provenances. Seeds from the YKL (Karayagaqi Township, Yining County) and YKG (G218, KashiTown, Yining County) regions were larger in size compared to seeds from other regions. Among the four measured nutritional components, crude fat had the highest content, especially in the YKL and YKG region. The results of correlation analysis showed that crude fat was negatively correlated with soluble sugar and soluble protein but significantly positively correlated with starch content. As longitude increased from east to west, the morphological characteristics gradually increased. Based on the principal component analysis of all the parameters of the seeds, the eight provenances could be classified into three groups. HM (Hami), TGS (S202, Gaochang District, Turpan), HYW (Wubao Town, Yizhou District, Hami), TQQ (Qiquanhu Town, Turpan), and TLF (Turpan) were a group with higher soluble protein, soluble sugar, and water content. YKL and YKG were in one group, which had larger seed grains with high crude fat and starch content. AKS (Aksu) was in a separate group. The protein fractions from seeds of eight regions were extracted using Osborne fractionation method, it was found that glutelin content was the highest, while albumin content was the lowest. After these proteins were analyzed by SDS-PAGE, the electrophoretic patterns showed that the protein molecular weights were relatively small, and there were differences in protein bands among different provenances. Conclusion According to the PCA results, the eight seed provenances could be divided into three groups. There were both geographically distant ones clustered into one group, and those close to each other were also divided into one group. There were differences in seed morphology, nutrient content and SDS-PAGE profiles among the different seed sources. This difference might be caused by a combination of geographic and climatic factors. In addition, YKL and YKG were roughly selected as good seed provenances, which provided a theoretical basis for the development of C. spinosa L. germplasm resources.

The present study examines the water quality in the Quaternary Mio-Plio-Quaternary aquifer of the mining basin of Gafsa using a hydrochemical approach and multivariate statistical methods, to assess groundwater mineralization processes. Results from the analysis of groundwater quality collected during the winter (January 2020) and summer (June 2021) seasons reveal a pronounced stability in geochemical parameters, emphasizing a noteworthy consistency in water composition between the two seasons, with the dominance of the Na-Ca-Mg-SO4-Cl facies, in addition to the fact that all year round these concentrations are beyond their respective WHO limits. Despite the intensive extractive and transformation phosphate industry, the prolonged interaction of water with geological formations is the primary factor controlling their high mineralization. This results from the dissolution of carbonates (calcite, dolomite), gypsum, and halite. The results of the PCA represent two correlation classes. Class 1 comprises major elements sulfate, chloride, sodium, magnesium, and calcium strongly correlated with electrical conductivity (EC) and total dissolved solids (TDS). This correlation is indicative of the water mineralization process. Class 2 includes major elements nitrate and potassium weakly correlated with (TDS) and (EC) As regards heavy metals, their concentrations fall consistently below their respective potability standards established by the WHO across all water sampling points. Meanwhile, fluoride (F-) concentrations exhibited values ranging from (1.6 mg·L−1 to 2.9 mg·L−1) in the winter of January 2020 and (1 to 2.9 mg·L−1) in the summer of June 2021, surpassing its WHO limit (1.5 mg·L−1) in almost all water samples. These findings allow us to conclude that the high mineralization of these waters is acquired due to the dissolution of carbonates (calcite, dolomite), gypsum, and halite due to their prolonged interaction with the geological formations. The deterioration of groundwater quality in the Gafsa mining basin associated with phosphate extraction and processing activities appears to be primarily due to the intensive exploitation of deep-water resources.

Introduction: To investigate the effects of different drip irrigation periods on soil microbial communities and functions. Methods: Increasing drip irrigation amount at the seedling (S), jointing (J), bell (B), tasseling (T) and grain filling (G) stages of maize were studied using no increase in irrigation amount as control (CK). Principal component analysis was conducted to comprehensively evaluate soil microbial quality following the different drip irrigation treatments. In addition, the characteristics of the community structure and the potential functional composition of soil bacteria and fungi were comparatively analyzed by combining amplicon sequencing and functional prediction methods. Results: The results indicated that MBNT15 was the most important genus for the classification of soil bacterial samples, Saitozyma was the most important genus for the classification of soil fungal samples, and fungi were more important than bacteria for the classification of soil microbial samples. Compared with fungal communities, bacterial communities exhibited high levels of functional diversity. The proportion of metabolism was the highest in the prediction of bacterial primary functions, and carbohydrate metabolism and amino acid metabolism were important functions in the prediction of bacterial secondary functions. BugBase phenotype prediction results showed that soil bacteria under B treatment had a higher number of aerobic bacteria and greater resistance to disease and stress. The J treatment had the highest number of bacteria with biofilm forms, and the J, S, and G treatments contained more potentially pathogenic bacteria but fewer stress-tolerant bacteria compared with the CK treatment. The number of Saprotroph was the largest and the number of Symbiotroph was the least. The relative abundances of Saprotroph, Pathotroph and Symbiotroph were 68.60%~74.33%, 15.76%~20.60% and 9.16%~11.13%, respectively. Discussion: The findings provide a reference for conserving water resources, improving maize yield, and predicting soil microbial metabolic potential and function by reflecting the richness of the soil microbial community structure in maize fields. [ABSTRACT FROM AUTHOR]

Soil is a unique ecosystem with peculiar biodiversity that includes cyanobacteria and algae. Traditionally, cyanobacterial and algal cenoses were described mainly using the dominance approach, rarely based on the Braun-Blanquet method (floristic classification). More importantly, in both cases, the species of cyanobacteria and algae in communities were identified using classical methods (light microscopy) only. In this study, we present results of soil algal cenoses classification using the Braun-Blanquet approach based on species composition data obtained via an integrative approach. Characteristic tables include 19 out of 108 samples collected in the Jewish Autonomous Region, Primorsky Territory, and Sakhalin Region (Iturup Island) in 2018 and in 2020–2021. Twenty-five species of algae from four classes were identified in these sites. We described three new associations of algal communities—Coelastrelletum aeroterrestricae ass. nova, Vischerietum magnae ass. nova, Bracteacoccetum bullati ass. nova. PCA analysis corroborated the results of syntaxonomic analysis and revealed that Coelastrelletum aeroterrestricae inhabit soils with a high value of P; Vischerietum magnae inhabit soils with high value of soil organic carbon (SOC), N, and higher humidity; and Bracteacoccetum bullati inhabit soils with high K values. [ABSTRACT FROM AUTHOR]

Macrophage populations exist on a spectrum between the proinflammatory M1 and proresolution M2 states and have demonstrated the ability to reprogram between them after exposure to opposing polarization stimuli. Particulate matter (PM) has been repeatedly linked to worsening morbidity and mortality following respiratory infections and has been demonstrated to modify macrophage function and polarization. The purpose of this study was to determine whether diesel exhaust particles (DEP), a key component of airborne PM, would demonstrate polarization state-dependent effects on human monocyte-derived macrophages (hMDMs) and whether DEP would modify macrophage reprogramming. CD14 þ CD16 monocytes were isolated from the blood of healthy human volunteers and differentiated into macrophages with macrophage colony-stimulating factor (M-CSF). Resulting macrophages were left unpolarized or polarized into the proresolution M2 state before being exposed to DEP, M1-polarizing conditions (IFN-γ and LPS), or both and tested for phagocytic function, secretory profile, gene expression patterns, and bioenergetic properties. Contrary to previous reports, we observed a mixed M1/M2 phenotype in reprogrammed M2 cells when considering the broader range of functional readouts. In addition, we determined that DEP exposure dampens phagocytic function in all polarization states while modifying bioenergetic properties in M1 macrophages preferentially. Together, these data suggest that DEP exposure of reprogrammed M2 macrophages results in a highly inflammatory, highly energetic subpopulation of macrophages that may contribute to the poor health outcomes following PM exposure during respiratory infections. NEW & NOTEWORTHY We determined that reprogramming M2 macrophages in the presence of diesel exhaust particles (DEP) results in a highly inflammatory mixed M1/M2 phenotype. We also demonstrated that M1 macrophages are particularly vulnerable to particulate matter (PM) exposure as seen by dampened phagocytic function and modified bioenergetics. Our study suggests that PM causes reprogrammed M2 macrophages to become a highly energetic, highly secretory subpopulation of macrophages that may contribute to negative health outcomes observed in humans after PM exposure. [ABSTRACT FROM AUTHOR]

Background: Capparis spinosa L. is a typical desert plant that is resistant to high temperatures and drought, and at the same time is rich in medicinal and food values. The objective of this study is to explore the variations in nutrient composition, morphological characteristics, and SDS-PAGE patterns of caper seeds from different provenances, aiming to provide insights for the selection of superior seed provenances. Results: In this experiment, there were significant differences in the morphological characteristics and major nutritional components of caper seeds from different provenances. Seeds from the YKL (Karayagaqi Township, Yining County) and YKG (G218, KashiTown, Yining County) regions were larger in size compared to seeds from other regions. Among the four measured nutritional components, crude fat had the highest content, especially in the YKL and YKG region. The results of correlation analysis showed that crude fat was negatively correlated with soluble sugar and soluble protein but significantly positively correlated with starch content. As longitude increased from east to west, the morphological characteristics gradually increased. Based on the principal component analysis of all the parameters of the seeds, the eight provenances could be classified into three groups. HM (Hami), TGS (S202, Gaochang District, Turpan), HYW (Wubao Town, Yizhou District, Hami), TQQ (Qiquanhu Town, Turpan), and TLF (Turpan) were a group with higher soluble protein, soluble sugar, and water content. YKL and YKG were in one group, which had larger seed grains with high crude fat and starch content. AKS (Aksu) was in a separate group. The protein fractions from seeds of eight regions were extracted using Osborne fractionation method, it was found that glutelin content was the highest, while albumin content was the lowest. After these proteins were analyzed by SDS-PAGE, the electrophoretic patterns showed that the protein molecular weights were relatively small, and there were differences in protein bands among different provenances. Conclusion: According to the PCA results, the eight seed provenances could be divided into three groups. There were both geographically distant ones clustered into one group, and those close to each other were also divided into one group. There were differences in seed morphology, nutrient content and SDS-PAGE profiles among the different seed sources. This difference might be caused by a combination of geographic and climatic factors. In addition, YKL and YKG were roughly selected as good seed provenances, which provided a theoretical basis for the development of C. spinosa L. germplasm resources. [ABSTRACT FROM AUTHOR]

Seaweeds are used in traditional agriculture practices because of their beneficial effects. Recently, the rising demand for organically grown foods has amplified the use of organic fertilizers such as seaweed extracts. Despite their beneficial effects, few studies have reported information about compounds in seaweed extracts that are responsible for these benefits. Thus, the aim of this study was to evaluate the potential relationships between the components and secondary metabolites in four seaweed liquid extracts from Eisenia arborea, Macrocystis pyrifera, Padina caulescens, and Sargassum horridum and their biostimulant activity through changes in the germination, growth, and protein content of tomato seedlings (Solanum lycopersicum). The E. arborea and S. horridum extracts showed similar compositions (ash, organic carbon, bicarbonates, and chlorides), minerals (Ca, Fe, and Cu) and secondary metabolites (triterpenes and saponins), albeit with different component concentrations. The chemical composition of the P. caulescens extract was significantly different from those of the other extracts; it was characterized by high levels of total nitrogen, phenols, and carbohydrates. Almost all seaweed extracts had beneficial effects on seed germination and seedling length, except the S. horridum extract that inhibits germination. The hierarchical clustering plots and principal component analysis indicated that germination and protein content are related to the presence of sterol. Shoot length was closely related to mineral levels (K, Zn, B, Na) and the C:N ratio, whereas radicle length was closely related to the content of nitrogen, carbohydrates, phenols, and flavonoids in the seaweed extracts. However, the underlying mechanisms are still unclear and require further studies. [ABSTRACT FROM AUTHOR]

Background: The behavior of patients undergoing metabolic bariatric surgery (MBS) has been widely investigated to better understand their attitudes and to formulate effective advisory strategies. Although these elements have been studied separately, the magnitude of these behaviors still needs to be determined from a combined factors approach. This study aimed to identify the lifestyle patterns of patients who underwent MBS 5 or more years ago. Methods: In this observational study, 123 patients who underwent MBS at least 5 years ago were included. Anthropometric data, sociodemographic profile, dietary intake, physical activity level, sleeping, smoking, and alcohol consumption were collected for all participants. Lifestyle pattern was evaluated using principal component analysis (PCA) and the Kaiser-Meyer-Olkin (KMO) index was applied to evaluate data factorability. Results: PCA identified two patterns of behavior adopted by the participants in the late postoperative (PO) period (more than 5 years PO) of MBS. In the first pattern, a positive correlation was found between consumption of ultra-processed foods (r = 0.459), risky consumption of alcoholic beverages (r = 0.630), and tobacco use (r = 0.584). In the second, a positive correlation was observed between the consumption of unprocessed or minimally processed foods (r = 0.692) and more sleep time per day (r = 0.654). Conclusion: After 5 years of PO, combined behavior analysis revealed healthy and unhealthy lifestyle patterns, which points to the relevance of permanent clinical follow-up of these patients to ensure the best health status. [ABSTRACT FROM AUTHOR]

Tuberculosis (TB) continues to pose a global health challenge, exacerbated by the rise of drug-resistant strains. The development of new TB therapies is an arduous and time-consuming process. To expedite the discovery of effective treatments, computational structure-based drug repurposing has emerged as a promising strategy. From this perspective, conditionally essential targets present a valuable opportunity, and the mycobactin biosynthesis pathway stands out as a prime example highlighting the intricate response of Mycobacterium tuberculosis (Mtb) to changes in iron availability. This study focuses on the repurposing and revival of FDA-approved drugs (library) as potential inhibitors of MbtA, a crucial enzyme in mycobactin biosynthesis in Mtb conserved among all species of mycobacteria. The literature suggests this pathway to be associated with drug efflux pumps, which potentially contribute to drug resistance. This makes it a potential target for antitubercular drug discovery. Herein, we utilized cheminformatics and structure-based drug repurposing approaches, viz., molecular docking, dynamics, and PCA analysis, to decode the intermolecular interactions and binding affinity of the FDA-reported molecules against MbtA. Virtual screening revealed ten molecules with significant binding affinities and interactions with MbtA. These drugs, originally designed for different therapeutic indications (four antiviral, three anticancer, one CYP450 inhibitor, one ACE inhibitor, and one leukotriene antagonist), were repurposed as potential MbtA inhibitors. Furthermore, our study explores the binding modes and interactions between these drugs and MbtA, shedding light on the structural basis of their inhibitory potential. Principal component analysis highlighted significant motions in MbtA-bound ligands, emphasizing the stability of the top protein–ligand complexes (PLCs). This computational approach provides a swift and cost-effective method for identifying new MbtA inhibitors, which can subsequently undergo validation through experimental assays. This streamlined process is facilitated by the fact that these compounds are already FDA-approved and have established safety and efficacy profiles. This study has the potential to lay the groundwork for addressing the urgent global health challenge at hand, specifically in the context of combating antimicrobial resistance (AMR) and tuberculosis (TB). [ABSTRACT FROM AUTHOR]

Seaweeds are used in traditional agriculture practices because of their beneficial effects. Recently, the rising demand for organically grown foods has amplified the use of organic fertilizers such as seaweed extracts. Despite their beneficial effects, few studies have reported information about compounds in seaweed extracts that are responsible for these benefits. Thus, the aim of this study was to evaluate the potential relationships between the components and secondary metabolites in four seaweed liquid extracts from Eisenia arborea, Macrocystis pyrifera, Padina caulescens, and Sargassum horridum and their biostimulant activity through changes in the germination, growth, and protein content of tomato seedlings (Solanum lycopersicum). The E. arborea and S. horridum extracts showed similar compositions (ash, organic carbon, bicarbonates, and chlorides), minerals (Ca, Fe, and Cu) and secondary metabolites (triterpenes and saponins), albeit with different component concentrations. The chemical composition of the P. caulescens extract was significantly different from those of the other extracts; it was characterized by high levels of total nitrogen, phenols, and carbohydrates. Almost all seaweed extracts had beneficial effects on seed germination and seedling length, except the S. horridum extract that inhibits germination. The hierarchical clustering plots and principal component analysis indicated that germination and protein content are related to the presence of sterol. Shoot length was closely related to mineral levels (K, Zn, B, Na) and the C:N ratio, whereas radicle length was closely related to the content of nitrogen, carbohydrates, phenols, and flavonoids in the seaweed extracts. However, the underlying mechanisms are still unclear and require further studies.

This study aimed to evaluate the postharvest characteristics of edible fresh white shimeji mushrooms under different UV-C radiation doses. The experimental design used was fully randomized, in a 5 × 8 factorial scheme (UV–C radiation dose: 0 (control), 1, 2, 3, and 4 kJ m−2 x day of analysis), with 3 replications of 70 ± 1 g mushrooms each. After exposure to different doses, they were stored at 2 ± 0.5 °C and 60 ± 3.8 % RH. Data were subjected to permutational multivariate analysis (PERMANOVA) (p ≤ 0.05). There was no significance for interaction, nor the factor day, only for the UV-C radiation doses factor. Regarding PCA, among the doses applied, the dose of 2 kJ m−2 was effective in maintaining the quality of mushrooms with greater lightness, greater whiteness index, a greater amount of total extractable polyphenols, and total antioxidant activity. In conclusion, the dose of 2 kJ m−2 was effective in maintaining the postharvest quality of white shimeji mushrooms.

The coastal waters of Ambon Island have quite diverse ecosystems that allow for the presence of various organisms, one of which is gastropods. This study aimed to analyze the correlation of water parameters and Pb in sediment with the diversity of gastropods. The research was carried out by observing the density, water quality parameters, and Pb metal in the sediments. Water parameters were measured in situ and analyzed in the laboratory. The distribution of gastropods was analyzed using Principal Component Analysis (PCA). At the same time, correlation analysis was performed using the Pearson correlation approach in SPSS v.16. The results showed that the gastropods with the highest density in the waters of Ambon Island were Terebralia sulcata, Hebra corticata, and Nerita patula. While the species with the lowest density value were Nassarius olivaceus, Polinices didyma, Lunella cinerea, Conus eburneus, Cypraea isabella, Vexillum plicarium, and Columbella scripta. The Shannon-Wiener diversity index ranged from 1.253–2.622, and the diversity index ranged from 0.083–0.207. It was included in the low category because of the disturbance of water pollution and Pb metal in the sediments. Meanwhile, the dominance index ranged from 0.098 to 0.511, indicating species dominance at several observation stations. The waters' physical-chemical parameters strongly correlating with gastropod diversity are DO and Pb, with respective correlation values of r = 0.656 and r = –0.785. [ABSTRACT FROM AUTHOR]

Testing the feasibility of soil phytoremediation requires the development of models applicable on a large scale. Phytoremediation mechanisms include advanced rhizosphere biodegradation, phytoaccumulation, phytodegradation, and phytostabilization. The aim of this study was to evaluate the phytoremediation potential of the Sinapis alba. Identification of the factors influencing the extraction process of metals from contaminated soils in a laboratory system suitable for evaluating the phytoavailability of these metals in three solutions (M1-CaCl2, M2-DTPA, and M3-EDTA) included the following: distribution of metals in solution (Kd), soil properties and mobile fractions (SOC, CEC, pH), response surface methodology (RSM), and principal component analysis (PCA). The evaluation of the phytoremediation potential of the Sinapis alba plant was assessed using bioaccumulation coefficients (BACs). The accumulation of heavy metals in plants corresponds to the concentrations and soluble fractions of metals in the soil. Understanding the extractable metal fractions and the availability of metals in the soil is important for soil management. Extractable soluble fractions may be more advantageous in total metal content as a predictor of bioconcentrations of metals in plants. In this study, the amount of metal available in the most suitable extractors was used to predict the absorption of metals in the Sinapis alba plant. Multiple regression prediction models have been developed for estimating the amounts of As and Cd in plant organs. The performance of the predictive models generated based on the experimental data was evaluated by the adjusted coefficient of determination (aR2), model efficiency (RMSE), Durbin–Watson (DW) test, and Shapiro–Wilk (SW) test. The accumulation of the analyzed metals followed the pattern Root > Pods > Leaves > Seeds, stems > Flowers for As and Leaves > Root > Stem > Pods > Seeds > Flowers for Cd in soil contaminated with different metal concentrations. The obtained results showed a phytoremediation potential of the Sinapis alba plant. [ABSTRACT FROM AUTHOR]

To explore the alleviation effect and physiological mechanism of gibberellin (GA3) on the growth of ‘Paulownia 1201’ seedlings under NaCl stress, this study took ‘Paulownia 1201’ seedlings as materials, different NaCl and GA3 concentrations were set to determine and analyze the physiological indexes, photosynthetic indexes and ion transport capacity of ‘Paulownia 1201’ seedlings under NaCl stress alleviated by GA3. The results were as follows: (1) Under 150 mmol·L-1 NaCl stress, the growth of ‘Paulownia 1201’ seedlings decreased significantly (P

Wheat is a vital crop globally, essential for agriculture, economics, and food security. However, in arid and semi-arid conditions, wheat production faces significant challenges due to low water availability, uneven rainfall distribution, and high soil salinity. The germination and early seedling stages are particularly vulnerable to these stresses. Therefore, this study assessed 15 wheat genotypes for their tolerance to salinity stress during early growth stages, using a hydroponic system with four salt stress levels (0, 50, 100, and 150 mM NaCl). Significant differences were observed for genotype and salinity main effects and their interaction on all investigated traits, indicating considerable variability in the response to salt stress among the investigated wheat cultivars. High NaCl concentrations led to substantial reductions in measured parameters across genotypes, with some showing resilience while others exhibited heightened sensitivity. Stress tolerance indices, such as mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress tolerance index (STI) and yield index (YI), were identified as reliable indicators for selecting salt-tolerant wheat cultivars. Consequently, Sidi Okba (G11), Ziad (G12), Tamezghida (G13) and Zidane (G14) emerged as the most promising, displaying acceptable performance under both non-stress and salt-stress conditions. These genotypes could serve as valuable genetic resources for breeding programs aimed at enhancing wheat’s salinity tolerance, particularly in arid and semi-arid regions.

In this study, a modern principal component analysis (PCA) of the chemical properties of lunar soils was conducted. American and Soviet results acquired during the Apollo and Luna missions, respectively, were analyzed and compared. The chemical composition of the lunar soil was the focus of our analysis, the main aim of which was to assess any possible differences between the results provided by the missions in question. The results were visualized in two- and three-dimensional spaces. The use of PCA virtual variables enabled the chemical composition of the lunar soil to be fully visualized—something impossible to achieve using traditional techniques—and key similarities and differences among the properties of the lunar soil samples were determined. The sources of any differences were then conceptualized. The work reported in this paper offers new directions for future studies, especially research into the design of new lunar soil simulants for lunar construction and civil engineering programs.

The utilization of heterosis is of great significance in improving rice yield. To explore the physiological and genetic basis for high yield in indica–japonica hybrid rice, Zheyou18 (z18) and Yongyou12 (y12) were used as materials and compared with indica hybrid rice, Zheyou12 (z12); japonica hybrid rice, Liangyoupeijiu (LYPJ); and the conventional lines zhe04B (04B) and zhehui818 (h818) under seedling growth vigor, functional leaf morphology, chlorophyll content, yield component, panicle trait, and InDel heterosis analysis. Z18 and y12 showed the largest increase in plant height 6 d and 9 d after germination; the root dry weight of z18 was 31.2% and 42.0% higher than its parents on the 12th d. The length of functional leaves ranked in the middle, while the width was the largest, resulting in z18 and y12 having the largest leaf area. Yield components showed that z18 and y12 had the highest number of primary branches, spikelets, and grains, and grain yield, which was 58.1 g in z18, increased by 29.8% and 8.7%, respectively, in comparison with h818 and LYPJ. The InDel genetic distance was significantly positively correlated with single spike weight, with r reaching 0.771, making it the only consistent and most correlated among the seven traits. Therefore, we speculated that as the InDel genetic distance expands, heterosis mainly manifests in the increase in single spike weight. This study comprehensively explored the physiological mechanism of yield improvement in indica–japonica-hybrid rice and used InDel genetic distances to study the genetic basis of heterosis, which will be helpful for future rice yield improvement.

Volatile components are one key factor in sample identification, differential analysis, quality control and origin traceability. In order to identify and analyze the differences in volatile substances in different alfalfa seeds, this study used gas chromatography–ion mobility spectrometry (GC-IMS), combined with the Gallery Plot plug-in, and PCA, PLS-DA and other analysis methods. In this way, a comprehensive analysis of volatile components in 10 alfalfa seeds, including aerospace varieties, was conducted. A comparative analysis of the characteristics of different sample compounds using topographic maps and fingerprints led to isolation of 48 kinds of 54 volatile compounds. Among them, esters (9 types), olefins (8 types), ketones (8 types), alcohols (6 types) and aldehydes (6 types) were found to be the most abundant volatile compounds in alfalfa seeds. At the same time, PCA and PLS-DA analysis models showed that esters, ketones and alcohols were the main volatiles causing the differences among alfalfa seeds. Among them, the content of various substances in the ZT2 and ZT3 aerospace varieties were higher than that of other varieties, while the types and contents of volatiles in ZT1, ZM2 and GN3 were relatively low. Therefore, in combination with the differences in maturity of each sample, the 10 varieties of alfalfa were finally divided into three categories, and the varieties of the same series were basically classified into one category. This provides a basis and convenience for future seed screening, identification, traceability and forage breeding.

The data for provide evidences of the multi steady state of the human cell line HEK 293 was obtained from 2 L bioreactor continuous culture. A HEK 293 cell line transfected to produce soluble HER1 receptor was used. The bioreactor was operated at three different dilution rates in sequential manner. Daily samples of culture broth were collected, a total of 85 samples were processed. Viable cell concentration and culture viability was addressing by trypan blue exclusion method using a hemocytometer. Heterologous HER1 supernatant concentration was quantified by a specific ELISA and the metabolites by mass spectrometry coupled to a liquid chromatography.The primary data were collected in excel files, where it was calculated the kinetic and other variables by using mass balance and mathematical principles. It was compared the steady states behavior each other's to find out the existence of steady states’ multiplicity, taking into account the stationary phase with respect to the cell density (which means its coefficient of variation is less than 20 %).From the metabolic measurements by using Liquid Chromatography coupled to mass spectrometry (LC-MS), it was also built the data matrix with the specific rates of the 76 metabolites obtained. The data were processed and analyzed, using multivariate data asssnalysis (MVDA) to reduce the complexity and to find the main patterns present in the data.We describe also the full data of the metabolites not only for steady states but also in the time evolution, which could help others in terms of modeling and deep understanding of HEK293 metabolism, especially under different culture conditions.

Evaluating the consistency of herb injectable formulations could improve their product quality and clinical safety, particularly concerning the composition and content levels of trace ingredients. Panax notoginseng Saponins Injection (PNSI), widely used in China for treating acute cardiovascular diseases, contains low-abundance (10%–25%) and trace saponins in addition to its five main constituents (notoginsenoside R 1 , ginsenoside Rg 1 , ginsenoside Re, ginsenoside Rb 1 , and ginsenoside Rd). This study aimed to establish a robust analytical method and assess the variability in trace saponin levels within PNSI from different vendors and formulation types. To achieve this, a liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) method employing multiple ions monitoring (MIM) was developed. A "post-column valve switching" strategy was implemented to eliminate highly abundant peaks (NR 1 , Rg 1 , and Re) at 26 min. A total of 51 saponins in PNSI were quantified or relatively quantified using 18 saponin standards, with digoxin as the internal standard. This study evaluated 119 batches of PNSI from seven vendors, revealing significant variability in trace saponin levels among different vendors and formulation types. These findings highlight the importance of consistent content in low-abundance and trace saponins to ensure product control and clinical safety. Standardization of these ingredients is crucial for maintaining the quality and effectiveness of PNSI in treating acute cardiovascular diseases. [ABSTRACT FROM AUTHOR]

The phenolic composition and antioxidant activity of wine and fermented pomace (FP) from Cabernet Sauvignon grapes harvested at three ripening stages were evaluated using LC-MS/MS and spectrophotometric analyses. An investigation of grey mold's (Botrytis cinerea) influence on wine phenolic content modulation was conducted as well. Finally, the influence of the plant's ripening stage on the dynamics of the phenolic compounds extracted from wine and FP obtained from fully ripe grapes was evaluated. In this study, the content of catechin, epicatechin, quercetin, and p-coumaric, gallic, and syringic acids was analyzed. Wine and FP were obtained after extended maceration during the spontaneous and inoculated fermentation of fully ripe grapes. When comparing the wine and FP obtained from véraison, fully ripe, and overripe grapes, catechin was the most abundant in wine (40.13 ± 3.25 mg/L) and quercetin in FP (10.96 ± 0.14 mg/kg). A decrease in analyzed phenolic compounds was noticed in wine produced from grapes affected by Botrytis cinerea, and the highest depletion was found for quercetin. The use of a winemaking technique that involved differing maceration periods and inoculation using yeasts as well as spontaneous fermentation significantly modulated the phenolic content of derived wines and FP. The dynamics of the phenolic compounds extracted into wine, evaluated using a principal component analysis (PCA), highlighted contents of catechin and epicatechin. After a decrease in maceration, the PCA revealed a notable content of gallic and syringic acids, as well as quercetin, in samples of FP. This study offers a perspective for future research and the development of functional food with a high content of phenolic compounds originating from red grape products, such as wine and fermented pomace. [ABSTRACT FROM AUTHOR]

The agro-morphological characterization of local germplasm provides insight into existing diversity, enables the identification of desirable traits, and enhances crop improvement. The present study evaluated 96 kharif sorghum (Sorghum bicolor L. Moench) landraces and 6 checks using 20 agro-morphological traits at two locations, ICAR-IIMR in Hyderabad and Experimental Farm at Annamalai University in Annamalai Nagar, using alpha lattice design with 2 replications during 2021 kharif to assess genetic diversity. Results showed significant genetic variability among the 20 traits (P<0.01), providing opportunities for improvement. The high genotypic (GCV) and phenotypic components of variance (PCV) exhibited among the traits indicated their genetic determination and potential for improvement through breeding programs. High heritability and genetic advance also indicated the presence of additive genes, offering reliable improvement through trait selection. The correlation analysis showed a strong positive relationship between grain yield and several desirable traits, including panicle length, width, primary branch length, hundred seed weight, number of leaves, and total tillers per plant, indicating that grain yield can be improved by selecting accessions with desirable characteristics for these traits. The Cluster analysis using Euclidean distance revealed (four distinct clusters), with Cluster I being the most differentiated. These clusters may serve as valuable resources for hybridization programs. The PCA analysis indicated that the first three PCs accounted for 43.26% of the total variation and highlighted the key agro-morphological traits driving diversity. The results of this study demonstrated the significant genetic diversity among kharif sorghum landraces, providing a promising opportunity for varietal development programs. [ABSTRACT FROM AUTHOR]

Diversity lifts the productivity of any ecosystem as all the species have a vital role to play that is present within the ecosystem. The characterization is essential to delve into the ecological functions of microbial communities and discover the type of microorganisms present within the ecosystem. As microbial diversity in ecosystems responds to environmental disturbances, it functions as a marker to indicate the change in such ecosystems. Mine ecology differs significantly from other habitats due to the presence of acidic runoff. This paper provides insight into the diversity of cultivable bacteria isolated from lignite mines located in south Gujarat. A total of 67 heterotrophic isolates were successfully cultivated from the collected solid and water samples of the Rajpardi and Tadkeshwar Lignite mine sites. The isolates were characterized morphologically and biochemically, and intra- and extracellular enzyme synthesis were studied. Moreover, the relative density and frequency of cultivated isolates from the samples were calculated. The similarity and evenness of the heterotrophic isolated were studied by calculating diversity indices such as Shannon and Simpson. Alpha diversity was calculated in PAST software to analyse the similarity between the selected two mine sites. This research also explored the relationship between the variance in heterotrophic microbial diversity and substrate utilization richness of the studied lignite mines of Gujarat. [ABSTRACT FROM AUTHOR]

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Algal-dissolved organic matter (ADOM) is an important fraction of dissolved organic carbon (DOC) in eutrophic water. Although ADOM is known to be readily transformed by microbes, the role of sunlight-induced photochemical process and the interactions between two processes on ADOM transformation remains unclear. In this study, three types of treatments for ADOM, including photochemical process under natural solar light (L treatment), microbial process (M treatment), and the simultaneous photochemical plus microbial process (L&M), were performed for 18 days. Our results showed that M treatment was more effective for the loss of DOC, chromophoric DOM (CDOM) at short wavelengths (a254 and a280), than L treatment, while L treatment was more effective for the transformation of a350 and the fluorescent components of the ubiquitous humic-like component and the tryptophan-like component. Comparison in the decay kinetics of DOC and CDOM in the three treatments showed that the simultaneous photochemical and biological processes exhibited an inhibitory effect on DOC decay rate but not the percentage of labile DOC fraction. Higher relative abundance of protein-like substances was found after L&M treatment, while the relative abundance of humic-like substance and aromaticity increased after M treatment, and the low molecular-weight compounds were produced after L treatment. Our results emphasized the importance of photochemistry in processing ADOM to mediate the chemodiversity in natural water. [ABSTRACT FROM AUTHOR]

To explore the alleviation effect and physiological mechanism of gibberellin(GA3)on the growth of ‘Paulownia 1201' seedlings under NaCl stress, this study took ‘Paulownia 1201' seedlings as materials, different NaCl and GA3 concentrations were set to determine and analyze the physiological indexes, photosynthetic indexes and ion transport capacity of ‘Paulownia 1201' seedlings under NaCl stress alleviated by GA3. The results were as follows:(1)Under 150 mmol·L-1 NaCl stress, the growth of ‘Paulownia 1201' seedlings decreased significantly(P<0.05), which was more than 50%. The appropriate concentration of exogenous GA3 significantly increased the plant height, root length and biomass of ‘Paulownia 1201' seedling. The dry weight of 400 mg·L-1 GA3 treatment increased by 69.71% compared with A0.(2)With the increase of GA3 concentration, the activities of three antioxidant enzymes(SOD, POD, CAT)increased significantly; content of MDA decreased significantly; chlorophyll content and gas exchange parameters(Pn, Tr, Ci, Gs)were increased, and the photosynthetic efficiency increased; the contents of each ion(K+, Ca2+, Mg2+)increased first and then decreased. The Na+ contents in leaves and roots of 400 mg·L-1 GA3 treatment group decreased by 23.59% and 11.92% compared with A0.(3)Correlation analysis and PCA analysis showed that there was a correlation between each index, seedlings which treated with different concentrations of GA3 had obvious differences, and the best alleviating effect was found when the concentration of GA3 was 400 mg·L-1. In summary, foliar spraying GA3 can enhance the antioxidant capacity of ‘Paulownia 1201' seedlings under salt stress, alleviate the membrane lipid peroxidation reaction caused by the increase of reactive oxygen species under salt stress, promote the accumulation of photosynthesis and biomass of plants, reduce the absorption of Na+ by plants, promote the accumulation of nutrient ions, adding 400 mg·L-1 GA3 can improve the resistance of ‘Paulownia 1201' seedlings best. This study provides a basis for further understanding of Paulownia salt tolerance mechanism identification, and also provides a scientific reference for the development and utilization of Paulownia in saline land. [ABSTRACT FROM AUTHOR]

This study aimed to identify the most important variables of male and female beach handball workload demands and compare them by sex. A total of 92 elite Brazilian beach handball players (54 male: age 22.1 ± 2.6 years, height 1.8 ± 0.5 m, weight 77.6 ± 13.4 kg; and 38 female: age 24.4 ± 5.5 years, height 1.7 ± 0.5 m, weight 67.5 ± 6.5 kg) were analyzed in 24 official matches during a four-day congested tournament. From 250 variables measured by the inertial measurement unit, fourteen were extracted for analysis using Principal Component Analysis as selection criteria. Five Principal Components (PC) were extracted that explained 81.2--82.8% of total variance (overview of workload demands during beach handball). Specifically, 36.2--39.3% was explained by PC1 (DistanceExpl, Distance, Distance4--7 km/h, and Acc), 15--18% by PC2 (AccMax, Acc3--4 m/s, Dec4--3 m/s), 10.7--12.9% by PC3 (JumpsAvg Take-Off, JumpsAvg Landing and PLRT), 8--9.4% by PC4 (Distance >18.1 km/h, SpeedMax), and 6.7--7.7% by PC5 (HRAvg and Step Balance). Sex-related differences were found in the PC distribution of variables, as well as in selected variables (HRAvg, Dec4--3 m/s, Acc3--4 m/s, JumpsAvg Take-Off, JumpsAvg Landing, AccMax, Distance, Distance4--7 km/h, Acc, SpeedMax) with higher values in male players (p < .05). In conclusion, the sex-related PC distribution and workload demands in beach handball should consider for training design and injury prevention programs. [ABSTRACT FROM AUTHOR]

High-pressure homogenization (HPH) is considered an innovative and modern method of processing and preserving liquid and semi-liquid foods. The aim of this research was to examine the impact of HPH processing on the content of betalain pigments and physicochemical properties of beetroot juice. Combinations of the following HPH parameters were tested: the pressure used (50, 100, 140 MPa), the number of cycles (1 and 3) and the applied cooling or no cooling. The physicochemical analysis of the obtained beetroot juices was based on the determination of the extract, acidity, turbidity, viscosity and color values. Use of higher pressures and a greater number of cycles reduces the turbidity (NTU) of the juice. Moreover, in order to maintain the highest possible extract content and a slight color change of the beetroot juice, it was crucial to perform sample cooling after the HPH process. The quantitative and qualitative profiles of betalains have been also determined in the juices. In terms of the content of betacyanins and betaxanthins, the highest values were found in untreated juice at 75.3 mg and 24.8 mg per 100 mL, respectively. The high-pressure homogenization process resulted in a decrease in the content of betacyanins in the range of 8.5–20.2% and of betaxanthins in the range of 6.5–15.0%, depending on the parameters used. Studies have shown that that the number of cycles was irrelevant, but an increase in pressure from 50 MPa to 100 or 140 MPa had a negative effect on pigment content. Additionally, juice cooling significantly limits the degradation of betalains in beetroot juice. [ABSTRACT FROM AUTHOR]

Bistortae Rhizoma (Quanshen), a dried rhizome of Polygonum bistorta L., is edited in Chinese Pharmacopiea as only one of species of Polygonum. There are many adulterants were used as Quanshen such as "Eryeliao," "Taipingyangliao" and "Daogenliao" because of its remarkable functions. Previous researches had shown that there were not significant differences among them. Therefore, it is necessary to conduct a comprehensive authentication of these herbs samples. Analyses of the microscopic features of the powders and transverse sections of herbs are the most significant measures of accreditation of the quality of traditional Chinese medicine. In this study, microscopic observation of powders of the herbs and cross‐sectional tissues were performed by using common light microscopy, polarized light microscopy and combining PCA analysis to identify Quanshen and three adulterants. We found that calcium oxalate clusters can be a good microscopic marker index to distinguish Quabshen and Eryeliao. In addition, Quanshen and the other two can be further distinguished by the characteristics of cork layer cells and vascular bundles on transverse section of the rhizome. Our results showed that the approach, microscopic features and digital characterization, is efficient, convenient, and reliable. [ABSTRACT FROM AUTHOR]

Soil aggregation in forest ecosystem is considered as a significant physical process mainly influenced by manure, fertilizers or combination. This aggregation may directly alter the soil nutrient and their fractions in soil. So, soil samples were collected from two types of forests i.e. Natural Korean pine forests (NKPF) and Korean pine plantation (KPP) in order to know the quantities of organic and inorganic Phosphorus (P) amounts in different aggregate sizes viz. >5 mm, 2–5 mm, 0.25–2 mm, 5 mm) and decreased with the decreasing aggregate size, while other variables, i.e., NaOH-Pi, NaHCO3-Po, pH and T-N were not affected in aggregate size. H2O-Pi (48 ppm), NaHCO3-Pi (68 ppm), NaHCO3-Po (80 ppm), NaOH-Po (623 ppm), HCL-Po (67 ppm), SOC (20.36 ± 1.6) was estimated in medium fertilizer treatment. PCA analysis showed that spread/variance of data points on F1 (62.90%) is more than spread/variance of data points on F2 (57.74%) in NKPF and KPP, respectively, while correlation matrix showed high correlation between H2O-Pi and NaOH-Pi (0.63) and H2O-Pi and NaHCO3-Pi (0.63) while a strong negative correlation was present between Res-Pi and Po (−0.61). Moreover, litter inputs increased the organic-P fractions in soil particularly at medium treatment.

Soil is a unique ecosystem with peculiar biodiversity that includes cyanobacteria and algae. Traditionally, cyanobacterial and algal cenoses were described mainly using the dominance approach, rarely based on the Braun-Blanquet method (floristic classification). More importantly, in both cases, the species of cyanobacteria and algae in communities were identified using classical methods (light microscopy) only. In this study, we present results of soil algal cenoses classification using the Braun-Blanquet approach based on species composition data obtained via an integrative approach. Characteristic tables include 19 out of 108 samples collected in the Jewish Autonomous Region, Primorsky Territory, and Sakhalin Region (Iturup Island) in 2018 and in 2020–2021. Twenty-five species of algae from four classes were identified in these sites. We described three new associations of algal communities—Coelastrelletum aeroterrestricae ass. nova, Vischerietum magnae ass. nova, Bracteacoccetum bullati ass. nova. PCA analysis corroborated the results of syntaxonomic analysis and revealed that Coelastrelletum aeroterrestricae inhabit soils with a high value of P; Vischerietum magnae inhabit soils with high value of soil organic carbon (SOC), N, and higher humidity; and Bracteacoccetum bullati inhabit soils with high K values.

Considering that agricultural production needs to be adjusted to global climate changes, it is of particular importance to develop bread wheat germplasm with improved tolerance to abiotic stress conditions. Therefore, the aim of this research was to identify stable wheat genotypes with increased salinity stress tolerance. The experiment was conducted with 27 wheat genotypes, at two localities: Kumane (solonetz soil type) and Rimski Šančevi (chernozem soil type) during two growing seasons. A significant influence of genotype and environment, as well as G × E interaction, on the phenotypic expression of yield components was found. The factor of genotype had the largest effect on the variation in plant height (38.7%) and the smallest effect on the variation in the number of grains per spike (8.24%). The greatest influence of the environment (64.7%) and G × E interaction (17.44%) was found in the variation in the number of grains per spike. Salinity conditions contributed the most to the decrease in the number of grains per spike (30%), which is considered the best indicator of the impact of stress on the plant. The smallest decrease in the number of grains per spike was recorded in the genotypes Šumadija (16.0%) and Harmonija (18.8%). However, AMMI and PCA analyses showed that the genotype Harmonija is characterized by a higher value of the number of grains per spike and greater stability. The genotypes Renesansa, Jugoslavija, Bankut 1205, and Harmonija were characterized by the smallest reduction in plant height under salinity conditions, among which Jugoslavija and Renesansa exhibited high stability and lower trait values. The smallest reduction in spike length was found in the genotypes Jugoslavija and Šumadija, which exhibited the specific adaptability to salinity stress. In terms of salinity tolerance, the genotypes Šumadija, Harmonija, and Renesansa are considered valuable genetic resources in breeding programs.