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228 results on '"Oria Marc"'

8. Noggin-Loaded PLA/PCL Patch Inhibits BMP-Initiated Reactive Astrogliosis.

Author

Hawes, James, Gonzalez-Manteiga, Ana, Murphy, Kendall P., Sanchez-Petidier, Marina, Moreno-Manzano, Victoria, Pathak, Bedika, Lampe, Kristin, Lin, Chia-Ying, Peiro, Jose L., and Oria, Marc

Subjects
NEURAL stem cells, BONE morphogenetic proteins, BIOMEDICAL materials, FETAL surgery, MORPHOGENESIS
Abstract

Myelomeningocele (MMC) is a congenital birth defect of the spine and spinal cord, commonly treated clinically through prenatal or postnatal surgery by repairing the unclosed spinal canal. Having previously developed a PLA/PCL polymer smart patch for this condition, we aim to further expand the potential therapeutic options by providing additional cellular and biochemical support in addition to its mechanical properties. Bone morphogenetic proteins (BMPs) are a large class of secreted factors that serve as modulators of development in multiple organ systems, including the CNS. We hypothesize that our smart patch mitigates the astrogenesis induced, at least partly, by increased BMP activity during MMC. To test this hypothesis, neural stem or precursor cells were isolated from rat fetuses and cultured in the presence of Noggin, an endogenous antagonist of BMP action, with recombinant BMPs. We found that the developed PLA/PCL patch not only serves as a biocompatible material for developing neural stem cells but was also able to act as a carrier for BMP–Notch pathway inhibitor Noggin, effectively minimizing the effect of BMP2 or BMP4 on NPCs cultured with the Noggin-loaded patch. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Comparison of Amnio‐Exchange With a Novel Synthetic Amniotic Fluid Versus Commercially Used Fluids for Fetal Therapy: An In Vivo Rodent Model.

Author

Martin, Samuel, Peiro, Jose L., Oria, Marc, and Forde, Braxton

Abstract

Objective: Normal Saline (NS) and Lactated Ringer's (LR) damage human amniotic epithelium in vitro when compared with a synthetic amniotic fluid (Amnio‐well, AW). We sought to evaluate the effect of amnio‐exchange with NS, LR, and AW in vivo. Methods: On day E17.5, pregnant rats underwent amnio‐exchange with NS, LR, or AW. Fetuses in each pregnant rat that did not undergo amnio‐exchange acted as controls. Amnions were harvested at E20.5 and ultrastructure evaluated via electron microscopy. Protein levels of cleaved matrix metalloproteinase 9 (MMP9) and collagen 1 (Col1a) were evaluated via Western Blot. Connexin‐43 expression was evaluated via immunofluorescence (IF). Results: There was an increase in amnion microfractures and epithelial cellular shrinkage with NS and LR compared with control and AW. The cleaved MMP9/Col1 ratio was increased 3.9‐fold in NS (p < 0.001) and 4.5‐fold LR (p = 0.0201) relative to control, whereas AW expression was similar to control (p = 0.636). Connexin‐43 was also increased on IF in NS and LR relative to AW (mean gray intensity 26.5 ± 4.5, 26.5 ± 6.7, 19.2 ± 3.4, p < 0.001). Conclusion: Amnio‐exchange with NS and LR led to increased amniotic microfractures and collagen degradation compared with synthetic amniotic fluid. Larger models are warranted to validate or refute these findings. [ABSTRACT FROM AUTHOR]

Published
2024
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35. Fetal Tracheal Occlusion Increases Lung Basal Cells via Increased Yap Signaling

Author

Serapiglia, Vincent, Stephens, Chad A., Joshi, Rashika, Aydin, Emrah, Oria, Marc, Marotta, Mario, Peiro, Jose L., Varisco, Brian M., Universitat Autònoma de Barcelona, Serapiglia, Vincent, Stephens, Chad A., Joshi, Rashika, Aydin, Emrah, Oria, Marc, Marotta, Mario, Peiro, Jose L., Varisco, Brian M., and Universitat Autònoma de Barcelona

Abstract

Fetal endoscopic tracheal occlusion (FETO) is an emerging surgical therapy for congenital diaphragmatic hernia (CDH). Ovine and rabbit data suggested altered lung epithelial cell populations after tracheal occlusion (TO) with transcriptomic signatures implicating basal cells. To test this hypothesis, we deconvolved mRNA sequencing (mRNA-seq) data and used quantitative image analysis in fetal rabbit lung TO, which had increased basal cells and reduced ciliated cells after TO. In a fetal mouse TO model, flow cytometry showed increased basal cells, and immunohistochemistry demonstrated basal cell extension to subpleural airways. Nuclear Yap, a known regulator of basal cell fate, was increased in TO lung, and Yap ablation on the lung epithelium abrogated TO-mediated basal cell expansion. mRNA-seq of TO lung showed increased activity of downstream Yap genes. Human lung specimens with congenital and fetal tracheal occlusion had clusters of subpleural basal cells that were not present in the control. TO increases lung epithelial cell nuclear Yap, leading to basal cell expansion

Published
2022

43. Experimental Maternal Cytokine Prophylaxis Protects from Fetal Neural Tube Teratogenesis Through an NK Cell Mediated Mechanism

Author

Jose L. Peiro, Catherine R. Redden, Amir A. Alhajjat, Hee Kap Kang, Oria Marc, Aimen F. Shaaban, Katherine C. Ott, Lucas E Turner, and Soner Duru

Subjects
Fetus, Cytokine, medicine.anatomical_structure, Mechanism (biology), business.industry, medicine.medical_treatment, Pediatrics, Perinatology and Child Health, medicine, Neural tube, business, Cell mediated immunity, Cell biology
Published
2021
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