48 results on '"Ngandjio A"'
Search Results
2. First report of New Delhi Metallo-Beta-Lactamase-5 (NDM-5) - producing Escherichia coli isolates from water environment in Cameroon
- Author
-
Megueya, Armelle Leslie, primary, Makuetche, Kévine, additional, Scaccia, Nazareno, additional, Costa, Silvia Figueiredo, additional, Foncesca, Joyce Vanessa da Silva, additional, Komurian–Pradel, Florence, additional, Tchatchouang, Serges, additional, Simo, Pierrette, additional, Tchatchueng, Jules, additional, Nono, Arsène Djoko, additional, Ngang, Essia, additional, Ngandjio, Antoinette, additional, and Nzouankeu, Ariane, additional
- Published
- 2023
- Full Text
- View/download PDF
3. Genomic history of the seventh pandemic of cholera in Africa
- Author
-
Weill, François-Xavier, Domman, Daryl, Njamkepo, Elisabeth, Tarr, Cheryl, Rauzier, Jean, Fawal, Nizar, Keddy, Karen H., Salje, Henrik, Moore, Sandra, Mukhopadhyay, Asish K., Bercion, Raymond, Luquero, Francisco J., Ngandjio, Antoinette, Dosso, Mireille, Monakhova, Elena, Garin, Benoit, Bouchier, Christiane, Pazzani, Carlo, Mutreja, Ankur, Grunow, Roland, Sidikou, Fati, Bonte, Laurence, Breurec, Sébastien, Damian, Maria, Njanpop-Lafourcade, Berthe-Marie, Sapriel, Guillaume, Page, Anne-Laure, Hamze, Monzer, Henkens, Myriam, Chowdhury, Goutam, Mengel, Martin, Koeck, Jean-Louis, Fournier, Jean-Michel, Dougan, Gordon, Grimont, Patrick A. D., Parkhill, Julian, Holt, Kathryn E., Piarroux, Renaud, Ramamurthy, Thandavarayan, Quilici, Marie-Laure, and Thomson, Nicholas R.
- Published
- 2017
4. First report of New Delhi metallo-beta-lactamase-5 (NDM-5)-producing Escherichia coli isolates from water environment in Cameroon
- Author
-
Megueya, Armelle Leslie, Makuetche, Kévine, Scaccia, Nazareno, Costa, Silvia Figueiredo, da Silva Foncesca, Joyce Vanessa, Komurian-Pradel, Florence, Tchatchouang, Serges, Simo, Pierrette, Tchatchueng, Jules, Nono, Arsène Djoko, Ngang, Essia, Ngandjio, Antoinette, and Nzouankeu, Ariane
- Published
- 2024
- Full Text
- View/download PDF
5. Author Correction: Genomic analysis of sewage from 101 countries reveals global landscape of antimicrobial resistance (Nature Communications, (2022), 13, 1, (7251), 10.1038/s41467-022-34312-7)
- Author
-
Munk, Patrick, Brinch, Christian, Møller, Frederik Duus, Petersen, Thomas N., Hendriksen, Rene S., Seyfarth, Anne Mette, Kjeldgaard, Jette S., Svendsen, Christina Aaby, van Bunnik, Bram, Berglund, Fanny, Bego, Artan, Power, Pablo, Rees, Catherine, Lambrinidis, Dionisia, Neilson, Elizabeth Heather Jakobsen, Gibb, Karen, Coventry, Kris, Collignon, Peter, Cassar, Susan, Allerberger, Franz, Begum, Anowara, Hossain, Zenat Zebin, Worrell, Carlon, Vandenberg, Olivier, Pieters, Ilse, Victorien, Dougnon Tamègnon, Gutierrez, Angela Daniela Salazar, Soria, Freddy, Grujić, Vesna Rudić, Mazalica, Nataša, Rahube, Teddie O., Tagliati, Carlos Alberto, Rodrigues, Dalia, Oliveira, Guilherme, de Souza, Larissa Camila Ribeiro, Ivanov, Ivan, Juste, Bonkoungou Isidore, Oumar, Traoré, Sopheak, Thet, Vuthy, Yith, Ngandjio, Antoinette, Nzouankeu, Ariane, Olivier, Ziem A.Abah Jacques, Yost, Christopher K., Kumar, Pratik, Brar, Satinder Kaur, Tabo, Djim Adjim, Adell, Aiko D., Paredes-Osses, Esteban, Koopmans, Marion, and Virology
- Abstract
In this article, the author name Antoinette Ngandjio was incorrectly written as Antoinette Ngandijo. In this article, the affiliation details for Author Sara Cuadros-Orellana were incorrectly given as ‘Centro de Biotecnologνa de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’ but should have been ‘Universidad Catolica del Maule, Centro de Biotecnología de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Talca, Chile’. The original article has been corrected.
- Published
- 2023
6. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
- Author
-
Murielle Baltazar, Antoinette Ngandjio, Kathryn Elizabeth Holt, Elodie Lepillet, Maria Pardos de la Gandara, Jean-Marc Collard, Raymond Bercion, Ariane Nzouankeu, Simon Le Hello, Gordon Dougan, Marie-Christine Fonkoua, and François-Xavier Weill
- Subjects
Salmonella enterica serotype Typhi ,bacteria ,multidrug resistance ,MDR ,pulsed-field gel electrophoresis ,PFGE ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
- Full Text
- View/download PDF
7. Histoplasmosis in HIV-Infected Persons, Yaoundé, Cameroon
- Author
-
Christine E. Mandengue, Antoinette Ngandjio, and Paul J.A. Atangana
- Subjects
Cameroon ,HIV/AIDS and other retroviruses ,tuberculosis ,histoplasmosis ,fungi ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2015
- Full Text
- View/download PDF
8. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
- Author
-
Breurec, S., Guessennd, N., Timinouni, M., Le, T.T.H., Cao, V., Ngandjio, A., Randrianirina, F., Thiberge, J.M., Kinana, A., Dufougeray, A., Perrier-Gros-Claude, J.D., Boisier, P., Garin, B., and Brisse, S.
- Published
- 2013
- Full Text
- View/download PDF
9. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010.
- Author
-
Rolf S Kaas, Antoinette Ngandjio, Ariane Nzouankeu, Achiraya Siriphap, Marie-Christine Fonkoua, Frank M Aarestrup, and Rene S Hendriksen
- Subjects
Medicine ,Science - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
- Published
- 2016
- Full Text
- View/download PDF
10. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
- Author
-
Marie-Christine Fonkoua, M. Massongo, W. Mbanzouen, D. Barger, L. Ngando, Ariane Nzouankeu, J. Tchatchueng, E.W. Pefura Yone, M. C. Tejiokem, A. Ngandjio, Université de Yaoundé I, Jamot hospital, Service de bactériologie [Yaoundé, Cameroun], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Laboratoire d'Epidémiologie et de Santé Publique, Bordeaux population health (BPH), and Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
0301 basic medicine ,Adult ,Male ,Veterinary medicine ,Salmonella ,Article Subject ,Adolescent ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Drug resistance ,Microbial Sensitivity Tests ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Antibiotic resistance ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Drug Resistance, Bacterial ,medicine ,Humans ,030212 general & internal medicine ,Cameroon ,Medical prescription ,Child ,Retrospective Studies ,General Immunology and Microbiology ,biology ,Bacteria ,Pseudomonas aeruginosa ,business.industry ,Infant ,General Medicine ,Bacterial Infections ,Acinetobacter ,Middle Aged ,biology.organism_classification ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,3. Good health ,Anti-Bacterial Agents ,Multiple drug resistance ,Child, Preschool ,Medicine ,Female ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,business ,Research Article - Abstract
Introduction. Antimicrobial resistance represents a growing public health threat. One of the World Health Organization’s strategic objectives is “strengthening knowledge through surveillance and research.” Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon. Methods. We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data were extracted from the CPC’s laboratory data information system software and then managed and analyzed using R. Bacterial resistance rates were calculated in each year and compared using chi-square or Fisher’s tests, and relative changes were calculated. Outcomes included acquired resistance (AR), WHO priority resistant pathogens, some specific resistances of clinical interest, and resistance patterns (multi, extensively, and pan drug resistances) for five selected pathogens. Results. A total of 10,218 isolates were analyzed. The overall AR rate was 96.0% (95% CI: 95.4–96.6). Most of WHO priority bacterial resistance rates increased from 2010 to 2017. The most marked increases expressed as relative changes concerned imipenem-resistant Acinetobacter (6.2% vs. 21.6%, +248.4%, p = 0.02 ), imipenem-resistant Pseudomonas aeruginosa (13.5% vs. 23.5%, +74.1%, p < 0.01 ), 3rd generation-resistant Enterobacteriaceae (23.8% vs. 40.4%, +65.8%, p < 10 − 15 ), methicillin-resistant Staphylococcus aureus (27.3% vs. 46.0%, +68.6%, p < 0.002 ), fluoroquinolone-resistant Salmonella (3.9% vs. 9.5%, +142.9%, p = 0.03 ), and fluoroquinolone-resistant Enterobacteriaceae (32.6% vs. 54.0%, +65.8%, p < 10 − 15 ). For selected pathogens, global multidrug resistance was high in 2010 and 2017 (74.9% vs. 78.0% +4.1%, p = 0.01 ), intensively drug resistance rate was 5.8% (7.0% vs. 4.7%; p = 0.07 ), and no pan drug resistance has been identified. Conclusion. Bacterial resistance to antibiotics of clinical relevance in Cameroon was high and appeared to increase between 2010 and 2017. There is a need for regular surveillance of antibacterial resistance to inform public health strategies and empirically inform prescription practices.
- Published
- 2021
11. Genomic history of the seventh pandemic of cholera in Africa
- Author
-
François-Xavier Weill, Karen H. Keddy, Mireille Dosso, Sandra Moore, Anne Laure Page, Renaud Piarroux, Jean Michel Fournier, Antoinette Ngandjio, Monakhova Ev, Julian Parkhill, Sébastien Breurec, Monzer Hamze, Nizar Fawal, Henrik Salje, Fati Sidikou, Elisabeth Njamkepo, Asish K. Mukhopadhyay, Maria Damian, Gordon Dougan, Kathryn E. Holt, Marie Laure Quilici, Daryl Domman, Raymond Bercion, Jean Rauzier, Martin A. Mengel, Cheryl L. Tarr, Patrick A. D. Grimont, Benoit Garin, Laurence Bonte, Goutam Chowdhury, Roland Grunow, Jean Louis Koeck, Berthe Marie Njanpop-Lafourcade, Guillaume Sapriel, Myriam Henkens, Carlo Pazzani, Christiane Bouchier, Ankur Mutreja, Nicholas R. Thomson, Francisco J. Luquero, Thandavarayan Ramamurthy, Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris] (IP), The Wellcome Trust Sanger Institute [Cambridge], Centers for Disease Control and Prevention [Atlanta] (CDC), Centers for Disease Control and Prevention, National Institute for Communicable Diseases [Johannesburg] (NICD), University of the Witwatersrand [Johannesburg] (WITS), Modélisation mathématique des maladies infectieuses - Mathematical modelling of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Hôpital de la Timone [CHU - APHM] (TIMONE), Université de la Méditerranée - Aix-Marseille 2, Institut Pasteur de Bangui, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Dakar, Epicentre [Paris] [Médecins Sans Frontières], Centre Pasteur du Cameroun, Institut Pasteur de Côte d'Ivoire, Rostov-on-Don Research Institute for Plague Control, Génomique (Plate-Forme) - Genomics Platform, Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), University of Cambridge [UK] (CAM), Translational Health Science and Technology Institute [Faridabad] (THSTI), Robert Koch Institute [Berlin] (RKI), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Cantacuzino Institute [Romania], Agence de Médecine Préventive, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université Libanaise, Médecins Sans Frontières Belgique, National Institute of Cholera and Enteric Diseases, Hôpital Bouffard - Centre Hospitalier des Armées Bouffard [Djibouti], Service de Santé des Armées, Centre National de Référence des Vibrions et du Choléra - National Reference Center Vibrios and Cholera (CNR), Biodiversité des Bactéries Pathogènes Émergentes, University of Melbourne, London School of Hygiene and Tropical Medicine (LSHTM), This study was supported by the Institut Pasteur and the Institut Pasteur International Network, the Institut de Veille Sanitaire, the French government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant no. ANR-10-LABX-62-IBEID), the Fondation Le Roch-Les Mousquetaires, the Wellcome Trust through grant 098051 to the Sanger Institute, and the Indian Council of Medical Research, New Delhi, India., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Bari Aldo Moro (UNIBA), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Weill, François-Xavier [0000-0001-9941-5799], Domman, Daryl [0000-0001-5389-9185], Njamkepo, Elisabeth [0000-0001-6791-6003], Tarr, Cheryl [0000-0001-6290-365X], Fawal, Nizar [0000-0002-9914-0315], Keddy, Karen H [0000-0001-8640-2235], Salje, Henrik [0000-0003-3626-4254], Bercion, Raymond [0000-0002-0466-0407], Luquero, Francisco J [0000-0003-0885-8418], Ngandjio, Antoinette [0000-0001-9777-3656], Dosso, Mireille [0000-0003-4744-8235], Monakhova, Elena [0000-0002-9216-7777], Bouchier, Christiane [0000-0003-3549-1543], Pazzani, Carlo [0000-0003-0414-2965], Grunow, Roland [0000-0001-8966-8469], Sidikou, Fati [0000-0002-0269-5454], Njanpop-Lafourcade, Berthe-Marie [0000-0001-6211-6402], Page, Anne-Laure [0000-0001-7952-3178], Koeck, Jean-Louis [0000-0002-6493-3905], Dougan, Gordon [0000-0003-0022-965X], Grimont, Patrick AD [0000-0002-6264-136X], Parkhill, Julian [0000-0002-7069-5958], Holt, Kathryn E [0000-0003-3949-2471], Piarroux, Renaud [0000-0002-4151-4134], Thomson, Nicholas R [0000-0002-4432-8505], and Apollo - University of Cambridge Repository
- Subjects
0301 basic medicine ,MESH: Pandemics ,Asia ,Lineage (genetic) ,Genomic data ,Zoology ,MESH: Vibrio cholerae O1 ,Biology ,MESH: Genome, Bacterial ,medicine.disease_cause ,Africa, Southern ,West africa ,03 medical and health sciences ,Cholera ,MESH: Cholera ,Phylogenetics ,MESH: Africa, Western ,parasitic diseases ,Pandemic ,medicine ,Humans ,MESH: Phylogeny ,Pandemics ,Phylogeny ,MESH: Africa, Eastern ,MESH: Humans ,Multidisciplinary ,MESH: Asia ,Transmission (medicine) ,MESH: Genomics ,Vibrio cholerae O1 ,Genomics ,Africa, Eastern ,medicine.disease ,3. Good health ,Africa, Western ,030104 developmental biology ,Vibrio cholerae ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,MESH: Africa, Southern ,Genome, Bacterial - Abstract
Wave upon wave of diseaseThe cholera pathogen,Vibrio cholerae, is considered to be ubiquitous in water systems, making the design of eradication measures apparently fruitless. Nevertheless, local and globalVibriopopulations remain distinct. Now, Weillet al.and Dommanet al.show that a surprising diversity between continents has been established. Latin America and Africa bear different variants of cholera toxin with different transmission dynamics and ecological niches. The data are not consistent with the establishment of long-term reservoirs of pandemic cholera or with a relationship to climate events.Science, this issue p.785, p.789
- Published
- 2017
12. Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
-
Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A., Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E., Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison E., Mason, Carl J., Page, Andrew J., Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carle, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Le Hello, Simon, Blaser, Martin J., Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja P., Ezernitchi, Analia V., Guigon, Ghislaine, Harris, Simon R., Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A.M., Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A., Ye, Changyun, Žemličková, Helena, Herpay, Mária, Grimont, Patrick A. D., Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn E., Brisse, Sylvain, Thomson, Nicholas R., and Weill, François-Xavier
- Published
- 2016
- Full Text
- View/download PDF
13. Histoplasmosis in HIV-infected persons, Yaounde, Cameroon
- Author
-
Mandengue, Christine E., Ngandjio, Antoinette, and Atangana, Paul J.A.
- Subjects
HIV patients -- Health aspects ,Histoplasmosis -- Health aspects ,Health - Abstract
To the Editor: In HIV-infected persons in Cameroon (Central Africa), histoplasmosis is still misdiagnosed as tuberculosis because of clinical similarities (1,2). These patients are automatically given presumptive antituberculous therapy, although [...]
- Published
- 2015
14. Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
- Author
-
Massongo, M., primary, Ngando, L., additional, Pefura Yone, E. W., additional, NZouankeu, Ariane, additional, Mbanzouen, W., additional, Fonkoua, M. C., additional, Ngandjio, A., additional, Tchatchueng, J., additional, Barger, D., additional, and Tejiokem, M. C., additional
- Published
- 2021
- Full Text
- View/download PDF
15. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
- Author
-
Ngandjio, Antoinette, Tchendjou, Patrice, Koki Ndombo, Paul, Gonsu Kamga, Hortense, and Fonkoua, Marie-Christine
- Published
- 2012
- Full Text
- View/download PDF
16. Trends in Gonococcal Antimicrobial Resistance in Cameroon: Before and After the Launching of the Gonococcal Antimicrobial Surveillance Program
- Author
-
Esther Sokeng, Carole Tayimetha, Ariane Nzouankeu, Marcelle Abanda, Marie-Christine Fonkoua, Gaëlle Tchouwa, and Antoinette Ngandjio
- Subjects
Antibiotic resistance ,business.industry ,Medicine ,Antimicrobial ,business ,Microbiology - Published
- 2016
17. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
- Author
-
Ngandjio, Antoinette, Clerc, Maithe, Fonkoua, Marie Christine, Thonnon, Jocelyn, Lunel, Françoise, Bébéar, Christiane, Bianchi, Anne, and de Barbeyrac, Bertille
- Published
- 2004
18. Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg
- Author
-
Pierrette Landrie Simo Tchuinte, Silvia Venditti, Olivier Barraud, Thibault Stalder, Christophe Dagot, Marie-Cécile Ploy, and Antoinette Ngandjio
- Subjects
0301 basic medicine ,Microbiology (medical) ,Luxembourg ,030106 microbiology ,Sewage ,Biology ,Real-Time Polymerase Chain Reaction ,Integron ,beta-Lactamases ,Aeromonas allosaccharophila ,Integrons ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,Plasmid ,Drug Resistance, Bacterial ,Humans ,Pharmacology (medical) ,Molecular Biology ,Bacteriological Techniques ,Acinetobacter ,business.industry ,High-Throughput Nucleotide Sequencing ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Hospitals ,Citrobacter freundii ,030104 developmental biology ,Infectious Diseases ,Gene cassette ,biology.protein ,bacteria ,Aeromonas ,France ,business ,Bacteria - Abstract
In this study, antibiotic resistance class 3 integrons in Gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. Two samples of hospital effluent from France and Luxembourg and one sample of sludge from a wastewater treatment plant in France were collected in 2010 and 2011. Bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative PCR. Integron gene cassette arrays and their genetic environments were analysed by next-generation sequencing. Three class 3 integron-positive isolates were detected, including Acinetobacter johnsonii LIM75 (French hospital effluent), Aeromonas allosaccharophila LIM82 (sludge) and Citrobacter freundii LIM86 (Luxembourg hospital effluent). The gene cassettes were all implicated in antibiotic (aminoglycoside and β-lactam) or antiseptic resistance. An oxacillinase gene cassette (blaOXA-10, blaOXA-368 or blaOXA-2) was found in each integron. All of the class 3 integrons were located on small mobilisable plasmids. This study highlights the role of class 3 integrons in the dissemination of clinically relevant antibiotic resistance genes, notably oxacillinase genes, in hospital effluent.
- Published
- 2016
19. Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg
- Author
-
Simo Tchuinte, Pierrette Landrie, Stalder, Thibault, Venditti, Silvia, Ngandjio, Antoinette, Dagot, Christophe, Ploy, Marie-Cécile, and Barraud, Olivier
- Published
- 2016
- Full Text
- View/download PDF
20. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
-
Elisabeth Njamkepo, Nizar Fawal, Alicia Tran-Dien, Jane Hawkey, Nancy Strockbine, Claire Jenkins, Kaisar A. Talukder, Raymond Bercion, Konstantin Kuleshov, Renáta Kolínská, Julie E. Russell, Lidia Kaftyreva, Marie Accou-Demartin, Andreas Karas, Olivier Vandenberg, Alison E. Mather, Carl J. Mason, Andrew J. Page, Thandavarayan Ramamurthy, Chantal Bizet, Andrzej Gamian, Isabelle Carle, Amy Gassama Sow, Christiane Bouchier, Astrid Louise Wester, Monique Lejay-Collin, Marie-Christine Fonkoua, Simon Le Hello, Martin J. Blaser, Cecilia Jernberg, Corinne Ruckly, Audrey Mérens, Anne-Laure Page, Martin Aslett, Peter Roggentin, Angelika Fruth, Erick Denamur, Malabi Venkatesan, Hervé Bercovier, Ladaporn Bodhidatta, Chien-Shun Chiou, Dominique Clermont, Bianca Colonna, Svetlana Egorova, Gururaja P. Pazhani, Analia V. Ezernitchi, Ghislaine Guigon, Simon R. Harris, Hidemasa Izumiya, Agnieszka Korzeniowska-Kowal, Anna Lutyńska, Malika Gouali, Francine Grimont, Céline Langendorf, Monika Marejková, Lorea A.M. Peterson, Guillermo Perez-Perez, Antoinette Ngandjio, Alexander Podkolzin, Erika Souche, Mariia Makarova, German A. Shipulin, Changyun Ye, Helena Žemličková, Mária Herpay, Patrick A. D. Grimont, Julian Parkhill, Philippe Sansonetti, Kathryn E. Holt, Sylvain Brisse, Nicholas R. Thomson, and François-Xavier Weill
- Subjects
Microbiology (medical) ,Immunology ,Genetics ,Cell Biology ,Applied Microbiology and Biotechnology ,Microbiology - Published
- 2016
21. Molecular characterization of multidrug resistant Salmonella from chicken and human in Yaounde
- Author
-
Awa Aidara-Kane, Marguerite Wouafo Wouafo, Marie-Christine Fonkoua, Thomas Njiné, Ariane Nzouankeu, and Antoinette Ngandjio
- Subjects
Salmonella ,Nalidixic acid ,biology ,Tetracycline ,Integron ,Antimicrobial ,medicine.disease_cause ,Virology ,Microbiology ,Multiple drug resistance ,Antibiotic resistance ,medicine ,biology.protein ,Pulsed-field gel electrophoresis ,medicine.drug - Abstract
Salmonella is one of the most common foodborne pathogens worldwide and chicken has been recognized as its main reservoir for human. The aim of this study was to investigate the occurrence of antimicrobial resistance genes in Salmonella isolates from chicken and human, and evaluate their genetic relationship. A total of 200 Salmonella strains (103 from Chicken and 97 from humans) were collected from 2006 to 2007 in Yaounde, and characterized for their antimicrobial susceptibility to a panel of 16 antimicrobials. Presence of antimicrobial resistance genes, class1 and class 2 integrons was investigated by PCR in Multidrug Resistant (MDR) isolates. Pulsed-Field Gel Electrophoresis (PFGE) analysis was used to investigate their genetic relatedness. Only serotypes Enteritidis, Hadar, Bareilly and II were recovered from both chicken and human. Overall, 16 isolates (8%) were susceptible to all antimicrobials including third generation cephalosporins and fluoroquinolons. Resistance was mostly observed to tetracycline (57%) in chicken isolates and to sulfonamides (78.3%) in human isolates. 78 (15.3%) strains were MDR. Class 1 integron was predominant in MDR Hadar and Typhimurium isolates. TEM-1 was the unique Extended Spectrum β-lactamase (ESBL) detected in the β-lactam resistant Typhimurium strain isolated from human. No qnr genes were identified in nalidixic acid resistant isolates. PFGE typing of Enteritidis isolates using XbaI restriction enzyme showed close genetic relationship between chicken and human isolates. For Hadar and Typhimurirum, variety of restriction patterns was observed. These results highlight the need for continuous surveillance of antimicrobial resistance in Salmonella isolates in Cameroon.
- Published
- 2016
22. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
-
Frank Møller Aarestrup, Antoinette Ngandjio, Rene S. Hendriksen, Achiraya Siriphap, Ariane Nzouankeu, Marie-Christine Fonkoua, and Rolf Sommer Kaas
- Subjects
Bacterial Diseases ,0301 basic medicine ,Disease reservoir ,Marine and Aquatic Sciences ,lcsh:Medicine ,Plant Science ,Pathology and Laboratory Medicine ,Plant Genetics ,medicine.disease_cause ,El Tor ,Genome ,Disease Outbreaks ,Geographical Locations ,Cholera ,Medicine and Health Sciences ,Plant Genomics ,Cameroon ,lcsh:Science ,Phylogeny ,Genetics ,Multidisciplinary ,biology ,Vibrio cholerae O1 ,Genomics ,Anti-Bacterial Agents ,Bacterial Pathogens ,Infectious Diseases ,Medical Microbiology ,Vibrio cholerae ,Pathogens ,Research Article ,Neglected Tropical Diseases ,Freshwater Environments ,Biotechnology ,Genotype ,Chad ,Microbial Sensitivity Tests ,Serogroup ,History, 21st Century ,Polymorphism, Single Nucleotide ,Microbiology ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,Phylogenetics ,Microbial Control ,Vibrio Cholerae ,parasitic diseases ,medicine ,Humans ,Microbial Pathogens ,Disease Reservoirs ,Vibrio ,Pharmacology ,Bacteria ,Ecology and Environmental Sciences ,lcsh:R ,Organisms ,Biology and Life Sciences ,Aquatic Environments ,Outbreak ,Sequence Analysis, DNA ,Bodies of Water ,Tropical Diseases ,medicine.disease ,biology.organism_classification ,Lakes ,030104 developmental biology ,People and Places ,Africa ,Earth Sciences ,Multilocus sequence typing ,Plant Biotechnology ,lcsh:Q ,Antimicrobial Resistance ,Genome, Bacterial ,Multilocus Sequence Typing - Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
- Published
- 2016
23. Prevalence and Antimicrobial Resistance of Salmonella Serotypes in Chickens from Retail Markets in Yaounde (Cameroon)
- Author
-
Junie Atangana Kinfack, Marie-Christine Fonkoua, Guy Ejenguele, Thomas Njiné, Ariane Nzouankeu, Antoinette Ngandjio, and Marguerite Wouafo
- Subjects
Microbiology (medical) ,Salmonella ,Meat ,Nalidixic acid ,Tetracycline ,Immunology ,Food Contamination ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Microbiology ,Drug Resistance, Multiple, Bacterial ,Ampicillin ,Clavulanic acid ,Drug Resistance, Bacterial ,Prevalence ,medicine ,Animals ,Cameroon ,Serotyping ,Pharmacology ,Amoxicillin ,Anti-Bacterial Agents ,Electrophoresis, Gel, Pulsed-Field ,Ciprofloxacin ,Streptomycin ,Chickens ,medicine.drug - Abstract
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonella serotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin-tetracycline-nalidixic acid. These results highlight once more the need for surveillance of Salmonella contamination in poultry.
- Published
- 2010
24. Trends in Gonococcal Antimicrobial Resistance in Cameroon: Before and After the Launching of the Gonococcal Antimicrobial Surveillance Program
- Author
-
Nzouankeu, Ariane, primary, Ngandjio, Antoinette, additional, Tayimetha, Carole, additional, Tchouwa, Gaëlle, additional, Abanda, Marcelle, additional, Sokeng, Esther, additional, and Fonkoua, Marie-Christine, additional
- Published
- 2016
- Full Text
- View/download PDF
25. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa
- Author
-
Pardos de la Gandara, Maria, Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos De La Gándara, María, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, Weill, François-Xavier, Institut Pasteur [Paris], Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), University of Melbourne, The Wellcome Trust Sanger Institute [Cambridge], Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium] (WIV-ISP), Institut Pasteur de Bangui, This study was supported by the Institut Pasteur, the Réseau International des Instituts Pasteur, the Institut de Veille Sanitaire, and the French Government Investissement d’Avenir Program (Integrative Biology of Emerging Infectious Diseases, Laboratory of Excellence, grant ANR-10-LABX-62-IBEID)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and Institut Pasteur [Paris] (IP)
- Subjects
Serotype ,MESH: Geography ,Epidemiology ,lcsh:Medicine ,Salmonella enterica serotype Typhi ,Drug resistance ,Salmonella typhi ,Plasmid ,Drug Resistance, Multiple, Bacterial ,MDR ,MESH: Genetic Variation ,bacteria ,0303 health sciences ,MESH: Guinea ,MESH: Microbial Sensitivity Tests ,Geography ,biology ,MESH: Polymorphism, Single Nucleotide ,MESH: Salmonella typhi ,Dispatch ,PFGE ,Anti-Bacterial Agents ,Infectious Diseases ,Salmonella enterica ,pulsed-field gel electrophoresis ,Microbiology (medical) ,Microbial Sensitivity Tests ,Polymorphism, Single Nucleotide ,Typhoid fever ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,multidrug resistance ,MESH: Anti-Bacterial Agents ,medicine ,Humans ,lcsh:RC109-216 ,MESH: Typhoid Fever ,Typhoid Fever ,030304 developmental biology ,Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa ,MESH: Humans ,030306 microbiology ,Haplotype ,lcsh:R ,Genetic Variation ,MESH: Drug Resistance, Multiple, Bacterial ,MESH: Haplotypes ,biology.organism_classification ,medicine.disease ,Virology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Multiple drug resistance ,Haplotypes ,Guinea ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
26. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
-
Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, Weill, François-Xavier, Njamkepo, Elisabeth, Fawal, Nizar, Tran-Dien, Alicia, Hawkey, Jane, Strockbine, Nancy, Jenkins, Claire, Talukder, Kaisar A, Bercion, Raymond, Kuleshov, Konstantin, Kolínská, Renáta, Russell, Julie E, Kaftyreva, Lidia, Accou-Demartin, Marie, Karas, Andreas, Vandenberg, Olivier, Mather, Alison AE, Mason, Carl J, Page, Andrew J, Ramamurthy, Thandavarayan, Bizet, Chantal, Gamian, Andrzej, Carles, Isabelle, Sow, Amy Gassama, Bouchier, Christiane, Wester, Astrid Louise, Lejay-Collin, Monique, Fonkoua, Marie-Christine, Hello, Simon Le, Blaser, Martin J, Jernberg, Cecilia, Ruckly, Corinne, Mérens, Audrey, Page, Anne-Laure, Aslett, Martin, Roggentin, Peter, Fruth, Angelika, Denamur, Erick, Venkatesan, Malabi, Bercovier, Hervé, Bodhidatta, Ladaporn, Chiou, Chien-Shun, Clermont, Dominique, Colonna, Bianca, Egorova, Svetlana, Pazhani, Gururaja GP, Ezernitchi, Analia V, Guigon, Ghislaine, Harris, Simon R, Izumiya, Hidemasa, Korzeniowska-Kowal, Agnieszka, Lutyńska, Anna, Gouali, Malika, Grimont, Francine, Langendorf, Céline, Marejková, Monika, Peterson, Lorea A M LA, Perez-Perez, Guillermo, Ngandjio, Antoinette, Podkolzin, Alexander, Souche, Erika, Makarova, Mariia, Shipulin, German A, Ye, Changyun, Zemlickova, Helena, Herpay, Mária, Grimont, Patrick A D PA, Parkhill, Julian, Sansonetti, Philippe, Holt, Kathryn KE, Brisse, Sylvain, Thomson, Nicholas R, and Weill, François-Xavier
- Abstract
info:eu-repo/semantics/published
- Published
- 2016
27. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
-
Kaas, Rolf Sommer, Ngandjio, Antoinette, Nzouankeu, Ariane, Siriphap, Achiraya, Fonkoua, Marie-Christine, Aarestrup, Frank Møller, Hendriksen, Rene S., Kaas, Rolf Sommer, Ngandjio, Antoinette, Nzouankeu, Ariane, Siriphap, Achiraya, Fonkoua, Marie-Christine, Aarestrup, Frank Møller, and Hendriksen, Rene S.
- Abstract
The prevalence of reported cholera was relatively low around the Lake Chad basin until 1991. Since then, cholera outbreaks have been reported every couple of years. The objective of this study was to investigate the 2010/2011 Vibrio cholerae outbreak in Cameroon to gain insight into the genomic make-up of the V. cholerae strains responsible for the outbreak. Twenty-four strains were isolated and whole genome sequenced. Known virulence genes, resistance genes and integrating conjugative element (ICE) elements were identified and annotated. A global phylogeny (378 genomes) was inferred using a single nucleotide polymorphism (SNP) analysis. The Cameroon outbreak was found to be clonal and clustered distant from the other African strains. In addition, a subset of the strains contained a deletion that was found in the ICE element causing less resistance. These results suggest that V. cholerae is endemic in the Lake Chad basin and different from other African strains.
- Published
- 2016
28. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students
- Author
-
Anne Bianchi, Marie Christine Fonkoua, Antoinette Ngandjio, Bertille de Barbeyrac, Christiane Bébéar, Jocelyn Thonnon, Françoise Lunel, and M. Clerc
- Subjects
Microbiology (medical) ,Genotype ,HpaII ,Restriction Mapping ,EcoRI ,Porins ,Chlamydia trachomatis ,Biology ,medicine.disease_cause ,Polymerase Chain Reaction ,Microbiology ,Male Urogenital Diseases ,medicine ,Humans ,Cameroon ,Typing ,Genetics ,General Medicine ,Chlamydia Infections ,Female Urogenital Diseases ,Restriction enzyme ,biology.protein ,Restriction fragment length polymorphism ,Nested polymerase chain reaction ,Polymorphism, Restriction Fragment Length - Abstract
Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.
- Published
- 2004
29. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1
- Author
-
Njamkepo, Elisabeth, primary, Fawal, Nizar, additional, Tran-Dien, Alicia, additional, Hawkey, Jane, additional, Strockbine, Nancy, additional, Jenkins, Claire, additional, Talukder, Kaisar A., additional, Bercion, Raymond, additional, Kuleshov, Konstantin, additional, Kolínská, Renáta, additional, Russell, Julie E., additional, Kaftyreva, Lidia, additional, Accou-Demartin, Marie, additional, Karas, Andreas, additional, Vandenberg, Olivier, additional, Mather, Alison E., additional, Mason, Carl J., additional, Page, Andrew J., additional, Ramamurthy, Thandavarayan, additional, Bizet, Chantal, additional, Gamian, Andrzej, additional, Carle, Isabelle, additional, Sow, Amy Gassama, additional, Bouchier, Christiane, additional, Wester, Astrid Louise, additional, Lejay-Collin, Monique, additional, Fonkoua, Marie-Christine, additional, Le Hello, Simon, additional, Blaser, Martin J., additional, Jernberg, Cecilia, additional, Ruckly, Corinne, additional, Mérens, Audrey, additional, Page, Anne-Laure, additional, Aslett, Martin, additional, Roggentin, Peter, additional, Fruth, Angelika, additional, Denamur, Erick, additional, Venkatesan, Malabi, additional, Bercovier, Hervé, additional, Bodhidatta, Ladaporn, additional, Chiou, Chien-Shun, additional, Clermont, Dominique, additional, Colonna, Bianca, additional, Egorova, Svetlana, additional, Pazhani, Gururaja P., additional, Ezernitchi, Analia V., additional, Guigon, Ghislaine, additional, Harris, Simon R., additional, Izumiya, Hidemasa, additional, Korzeniowska-Kowal, Agnieszka, additional, Lutyńska, Anna, additional, Gouali, Malika, additional, Grimont, Francine, additional, Langendorf, Céline, additional, Marejková, Monika, additional, Peterson, Lorea A.M., additional, Perez-Perez, Guillermo, additional, Ngandjio, Antoinette, additional, Podkolzin, Alexander, additional, Souche, Erika, additional, Makarova, Mariia, additional, Shipulin, German A., additional, Ye, Changyun, additional, Žemličková, Helena, additional, Herpay, Mária, additional, Grimont, Patrick A. D., additional, Parkhill, Julian, additional, Sansonetti, Philippe, additional, Holt, Kathryn E., additional, Brisse, Sylvain, additional, Thomson, Nicholas R., additional, and Weill, François-Xavier, additional
- Published
- 2016
- Full Text
- View/download PDF
30. The Lake Chad Basin, an Isolated and Persistent Reservoir of Vibrio cholerae O1: A Genomic Insight into the Outbreak in Cameroon, 2010
- Author
-
Kaas, Rolf S., primary, Ngandjio, Antoinette, additional, Nzouankeu, Ariane, additional, Siriphap, Achiraya, additional, Fonkoua, Marie-Christine, additional, Aarestrup, Frank M., additional, and Hendriksen, Rene S., additional
- Published
- 2016
- Full Text
- View/download PDF
31. Multidrug-Resistant Salmonella enterica, Serotype Typhi, Gulf of Guinea Region, Africa
- Author
-
Baltazar, M, Ngandjio, A, Holt, KE, Lepillet, E, Pardos de la Gandara, M, Collard, J-M, Bercion, R, Nzouankeu, A, Le Hello, S, Dougan, G, Fonkoua, M-C, Weill, F-X, Baltazar, M, Ngandjio, A, Holt, KE, Lepillet, E, Pardos de la Gandara, M, Collard, J-M, Bercion, R, Nzouankeu, A, Le Hello, S, Dougan, G, Fonkoua, M-C, and Weill, F-X
- Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid.
- Published
- 2015
32. Klebsiella pneumoniae resistant to third-generation cephalosporins in five African and two Vietnamese major towns: multiclonal population structure with two major international clonal groups, CG15 and CG258
- Author
-
Jean-David Perrier-Gros-Claude, V Cao, T A H Le, Benoit Garin, N Guessennd, Sylvain Brisse, M Timinouni, A Ngandjio, Jean-Michel Thiberge, Frédérique Randrianirina, Alfred Dieudonné Kinana, P. Boisier, A. Dufougeray, Sébastien Breurec, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Institut Pasteur de Côte d'Ivoire, Institut Pasteur du Maroc, Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Institut Pasteur d'Ho Chi Minh Ville, Centre Pasteur du Cameroun, Institut Pasteur de Madagascar, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Institut Pasteur [Paris], We thank Fatou Bintou Dieye (Institut Pasteur, Dakar, Senegal), Laure Diancourt and Patrice Courvalin (Institut Pasteur, Paris, France), Guillaume Arlet (Hoˆ pital Tenon, Paris, France) and Marie Christine Ploy (CHU Dupuytren, Limoges, France) for their contributions, as well as all the clinicians involved in this study., and Institut Pasteur [Paris] (IP)
- Subjects
Microbiology (medical) ,CTX-M-15 ,Veterinary medicine ,Genotype ,Klebsiella pneumoniae ,multilocus sequence typing ,beta-Lactam Resistance ,Microbiology ,03 medical and health sciences ,Intergenic region ,Antibiotic resistance ,Genetic variation ,parasitic diseases ,Humans ,antimicrobial resistance ,Developing Countries ,030304 developmental biology ,CG15 ,0303 health sciences ,Genetic diversity ,Molecular Epidemiology ,Molecular epidemiology ,biology ,030306 microbiology ,1. No poverty ,Genetic Variation ,General Medicine ,extended-spectrum β-lactamase ,biology.organism_classification ,extended-spectrum b-lactamase ,3. Good health ,Anti-Bacterial Agents ,Cephalosporins ,Klebsiella Infections ,Infectious Diseases ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Vietnam ,Genes, Bacterial ,Africa ,CG258 ,Multilocus sequence typing ,epidemiology ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
International audience; The molecular epidemiology of third-generation cephalosporin-resistant (3GC-R) Klebsiella pneumoniae in developing countries is poorly documented. From February 2007 to March 2008, we collected 135 3GC-R K. pneumoniae isolates from seven major towns in Maghreb (Morocco), West Africa (Senegal, Côte d'Ivoire), Central Africa (Cameroon), East Africa (Madagascar) and Southeast Asia (Vietnam). Their genetic diversity, assessed by multilocus sequence typing, was high (60 sequence types), reflecting multiclonality. However, two major clonal groups, CG15 (n = 23, 17% of isolates) and CG258 (n = 18, 13%), were detected in almost all participating centres. The two major clonal groups have previously been described in other parts of the world, indicating their global spread. The high diversity of enterobacterial repetitive intergenic consensus sequence-PCR banding patterns at the local level indicates that most isolates were epidemiologically unrelated. The isolates were characterized by the presence of multiple resistance determinants, most notably the concomitant presence of the aac(6')-Ib-cr, qnr and bla(CTX-M-15) genes in 61 isolates (45%) belonging to 31 sequence types. These isolates were detected across a large geographical area including Cameroon (n = 1), Vietnam (n = 4), Madagascar (n = 10), Côte d'Ivoire (n = 12), Morocco (n = 13) and Senegal (n = 21). These results have major implications for patient management and highlight a potential reservoir for resistance determinants.
- Published
- 2012
33. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
- Author
-
Paul Koki Ndombo, Antoinette Ngandjio, Marie-Christine Fonkoua, Hortense Gonsu Kamga, and Patrice Tchendjou
- Subjects
Serotype ,Male ,biology ,Adolescent ,business.industry ,Infant ,Salmonella enterica ,biology.organism_classification ,Virology ,Drug Resistance, Multiple ,Microbiology ,Infectious Diseases ,Anti-Infective Agents ,Pediatrics, Perinatology and Child Health ,Salmonella Infections ,Multi drug resistant ,Medicine ,Humans ,Female ,Cameroon ,Serotyping ,business - Published
- 2011
34. Multidrug-ResistantSalmonella entericaSerotype Typhi, Gulf of Guinea Region, Africa
- Author
-
Baltazar, Murielle, primary, Ngandjio, Antoinette, additional, Holt, Kathryn Elizabeth, additional, Lepillet, Elodie, additional, Pardos de la Gandara, Maria, additional, Collard, Jean-Marc, additional, Bercion, Raymond, additional, Nzouankeu, Ariane, additional, Le Hello, Simon, additional, Dougan, Gordon, additional, Fonkoua, Marie-Christine, additional, and Weill, François-Xavier, additional
- Published
- 2015
- Full Text
- View/download PDF
35. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
- Author
-
Françoise Lunel, Jocelyn Thonnon, Antoinette Ngandjio, Friede Njock, Marie Christine Fonkoua, Régis Pouillot, Christiane Bébéar, Bertille de Barbeyrac, M. Clerc, and Anne Bianchi
- Subjects
Microbiology (medical) ,Sexually transmitted disease ,Adult ,Male ,Genotype ,Chlamydiology and Rickettsiology ,Porins ,Chlamydia trachomatis ,medicine.disease_cause ,Risk Factors ,Prevalence ,Medicine ,Humans ,Chlamydiaceae ,Cameroon ,Genotyping ,Chlamydia ,Molecular epidemiology ,biology ,business.industry ,Chlamydia Infections ,biology.organism_classification ,medicine.disease ,Virology ,Chlamydiales ,Female ,business - Abstract
The prevalence of Chlamydia trachomatis infection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women ( P = 0.026) and a previous sexually transmitted infection in men ( P = 0.023). The most frequent genotypes determined by sequencing the omp1 genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
36. Emergence of multi-drug resistant Salmonella enterica serotype Stanleyville infections among children in Yaounde, Cameroon
- Author
-
Ngandjio, A., primary, Tchendjou, P., additional, Koki Ndombo, P., additional, Gonsu Kamga, H., additional, and Fonkoua, M.-C., additional
- Published
- 2011
- Full Text
- View/download PDF
37. Prevalence and Antimicrobial Resistance of Salmonella Serotypes in Chickens from Retail Markets in Yaounde (Cameroon)
- Author
-
Wouafo, Marguerite, primary, Nzouankeu, Ariane, additional, Kinfack, Junie Atangana, additional, Fonkoua, Marie-Christine, additional, Ejenguele, Guy, additional, Njine, Thomas, additional, and Ngandjio, Antoinette, additional
- Published
- 2010
- Full Text
- View/download PDF
38. Multiple contaminations of chickens with Campylobacter, Escherichia coli and Salmonella in Yaounde (Cameroon)
- Author
-
Nzouankeu, Ariane, primary, Ngandjio, Antoinette, additional, Ejenguele, Guy, additional, Njine, Thomas, additional, and Ndayo Wouafo, Marguerite, additional
- Published
- 2010
- Full Text
- View/download PDF
39. Antimicrobial Resistance and Molecular Characterization ofVibrio choleraeO1 During the 2004 and 2005 Outbreak of Cholera in Cameroon
- Author
-
Ngandjio, Antoinette, primary, Tejiokem, Mathurin, additional, Wouafo, Marguerite, additional, Ndome, Irene, additional, Yonga, Martial, additional, Guenole, Alain, additional, Lemee, Laure, additional, Quilici, Marie-Laure, additional, and Fonkoua, Marie-Christine, additional
- Published
- 2009
- Full Text
- View/download PDF
40. Multidrug-Resistant Salmonella enterica Serotype Typhi, Gulf of Guinea Region, Africa.
- Author
-
Baltazar, Murielle, Ngandjio, Antoinette, Holt, Kathryn Elizabeth, Lepillet, Elodie, Pardos de la Gandara, Maria, Collard, Jean-Marc, Bercion, Raymond, Nzouankeu, Ariane, Le Hello, Simon, Dougan, Gordon, Fonkoua, Marie-Christine, and Weill, François-Xavier
- Subjects
- *
MULTIDRUG resistance in bacteria , *SALMONELLA enterica serovar Typhi , *HAPLOTYPES , *PLASMIDS - Abstract
We identified 3 lineages among multidrug-resistant (MDR) Salmonella enterica serotype Typhi isolates in the Gulf of Guinea region in Africa during the 2000s. However, the MDR H58 haplotype, which predominates in southern Asia and Kenya, was not identified. MDR quinolone-susceptible isolates contained a 190-kb incHI1 pST2 plasmid or a 50-kb incN pST3 plasmid. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
41. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatis Infection and Genotyping of Isolated C. trachomatis Strains
- Author
-
Ngandjio, Antoinette, primary, Clerc, Maithe, additional, Fonkoua, Marie Christine, additional, Thonnon, Jocelyn, additional, Njock, Friede, additional, Pouillot, Regis, additional, Lunel, Françoise, additional, Bebear, Christiane, additional, de Barbeyrac, Bertille, additional, and Bianchi, Anne, additional
- Published
- 2003
- Full Text
- View/download PDF
42. First report of New Delhi metallo-beta-lactamase-5 (NDM-5)-producing Escherichia coliisolates from water environment in Cameroon
- Author
-
Megueya, Armelle Leslie, Makuetche, Kévine, Scaccia, Nazareno, Costa, Silvia Figueiredo, da Silva Foncesca, Joyce Vanessa, Komurian-Pradel, Florence, Tchatchouang, Serges, Simo, Pierrette, Tchatchueng, Jules, Nono, Arsène Djoko, Ngang, Essia, Ngandjio, Antoinette, and Nzouankeu, Ariane
- Published
- 2023
- Full Text
- View/download PDF
43. Antimicrobial Resistance and Molecular Characterization of Vibrio choleraeO1 During the 2004 and 2005 Outbreak of Cholera in Cameroon
- Author
-
Ngandjio, Antoinette, Tejiokem, Mathurin, Wouafo, Marguerite, Ndome, Irene, Yonga, Martial, Guenole, Alain, Lemee, Laure, Quilici, Marie-Laure, and Fonkoua, Marie-Christine
- Abstract
AbstractThere was an outbreak of cholera in Cameroon during 2004 and 2005; the epidemic began in Douala in January 2004 and spread throughout the south of the country. The World Health Organization (WHO) reported 8005 cases in 2004 and 2847 cases in 2005. Five hundred eighty-nine stool samples were received in the Pasteur Centre of Cameroon and 352 were microbiologically confirmed to be positive for Vibrio choleraeO1. Isolated strains were tested for their antimicrobial susceptibilities. All the strains were multidrug resistant and predominantly showed a common resistance pattern at the beginning of the outbreak. Tetracycline, recommended by the WHO for treating cholera in adults, was effective against all the strains tested. Cotrimoxazole (trimethoprim/sulfamethoxazole), previously a first-line treatment in children, was ineffective in vitrofor all the clinical isolates and was quickly replaced by amoxicillin. Ampicillin resistance emerged at the end of 2004 and was the leading resistance pattern observed in the second half of 2005. This therefore represented the second major resistance pattern. These two major resistance profiles were not associated with patient characteristics (sex and age) or to the geographic origin of strains. However, there was a highly significant relationship between resistance patterns and the year of isolation (p< 0.001). The strains possessed genes ctxAand ctxBencoding the two cholera toxin subunits and were very closely related, irrespective of their antimicrobial resistance patterns. They were not differentiated by molecular typing methods and gave similar ribotyping and pulsed-field gel electrophoresis patterns.
- Published
- 2009
- Full Text
- View/download PDF
44. Screening of Volunteer Students in Yaounde (Cameroon, Central Africa) for Chlamydia trachomatisInfection and Genotyping of Isolated C. trachomatisStrains
- Author
-
Ngandjio, Antoinette, Clerc, Maithe, Fonkoua, Marie Christine, Thonnon, Jocelyn, Njock, Friede, Pouillot, Regis, Lunel, Franc¸oise, Bebear, Christiane, de Barbeyrac, Bertille, and Bianchi, Anne
- Abstract
ABSTRACTThe prevalence of Chlamydia trachomatisinfection was 3.78% out of 1,277 volunteer students screened by direct fluorescence assay and Cobas Amplicor PCR. The infection was associated with the nonuse or inconsistent use of condoms in women (P= 0.026) and a previous sexually transmitted infection in men (P= 0.023). The most frequent genotypes determined by sequencing the omp1genes of 25 clinical isolates were E (44%) and F (20%), and some strains harbored mutations, but E genotype strains did not.
- Published
- 2003
- Full Text
- View/download PDF
45. Prevalence and Antimicrobial Resistance of SalmonellaSerotypes in Chickens from Retail Markets in Yaounde (Cameroon).
- Author
-
Marguerite Wouafo, Ariane Nzouankeu, Junie Atangana Kinfack, Marie-Christine Fonkoua, Guy Ejenguele, Thomas Njine, and Antoinette Ngandjio
- Subjects
- *
DISEASE prevalence , *ANTI-infective agents , *DRUG resistance in microorganisms , *SALMONELLA infections in poultry , *CHICKEN industry , *RETAIL industry , *DISEASE susceptibility - Abstract
From February 2006 to January 2007, 150 chickens were collected from eight retail markets in Yaounde, and 90 (60%) tested positive for Salmonella. Seventy-nine chickens were contaminated with only one Salmonellaserotype, 10 with two different serotypes, and 1 with four serotypes. The most prevalent serotypes were Enteritidis (47 strains) and Hadar (29 strains). The isolates were tested for their susceptibilities to amoxicillin, amoxicillin/clavulanic acid, cefoxitin, cefotaxime, gentamicin, streptomycin, tetracycline, chloramphenicol, sulfonamides, nalidixic acid, ciprofloxacin, and trimethoprim/sulfamethazole by disk diffusion assay. Minimum inhibitory concentrations of ampicillin, streptomycin, tetracycline, sulfonamides, and nalidixic acid were determined for the resistant strains by agar dilution method. Eleven isolates (10.7%) of the 103 tested were susceptible to all antimicrobials. Resistance was most observed to tetracycline (84.5%), streptomycin (44.7%), and nalidixic acid (34%). Forty-one isolates (39.8%) were multidrug resistant (resistant to three or more antimicrobials from different classes), of which 68.3% were Hadar and 21.9% Enteritidis. The most frequent resistant pattern in Hadar was streptomycin–tetracycline–nalidixic acid. These results highlight once more the need for surveillance of Salmonellacontamination in poultry. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
46. Class 3 integrons : machanistical and epidemiological aspects
- Author
-
Simo Tchuinte, Pierrette Landrie, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Université de Limoges, Marie-Cécile Ploy, Antoinette Brigitte Chikaha Tchouya-Ngandjio, and Olivier Barraud
- Subjects
Resistance aux antibiotiques ,Class 3 integrons ,Antibiotic resistance ,Effluents ,Intégrons de classe 3 ,Delftia ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Integrons are bacterial genetic elements able to capture and express genes embedded within gene cassettes. They are widely described among Gram-negative bacteria and generally confer a multidrug resistance phenotype. Resistance integrons (RI) play an important role in the acquisition of antibiotic resistance. There are 3 main classes of RI. Class 3 RI has been poorly studied class with less than ten fully IR3 characterized. Objectives of this thesis were (i) to conduct an epidemiological study of class 3 RI in France and Cameroon and (ii) to better understand the modes of expression of the integrase and cassettes of IR3. We described 3 new class 3 RI isolated from environmental bacteria belonging to genus Aeromonas, Acinetobacter and Citrobacter. Gene cassettes encoded resistance to betalactams, aminoglycosides and quaternary ammonium compounds. We also described IR3 from three Delftia strains (2 D.acidovorans and 1 D.tsuruhatensis) in Africa containing cassettes that do not encode antibiotic resistance. The fundamental part of the work showed that the PintI3(1) promoter is involved in the expression of the intI3 gene. Furthermore, we demonstrated that variants of the Pc promoter and variants of the PintI3(1) promoter are functional with different strengths. These results showed that the environment may constitute a reservoir of class 3 integrons and that these genetic elements could play an important role in the spread of the resistance in this ecosystem.; Les intégrons sont des supports génétiques bactériens de capture, d’expression et de dissémination de gènes de résistance aux antibiotiques sous forme de cassettes. Ils sont majoritairement décrits chez les bactéries à Gram négatif chez qui ils confèrent généralement un phénotype de multirésistance. Les intégrons de résistance (IR) jouent un rôle majeur dans l’acquisition de la résistance dans le monde bactérien. Il existe 3 principales classes d’IR ; les IR de classe 1, les IR de classe 2 et les IR de classe 3 (IR3). Contrairement aux 2 premières classes, les IR3 représentent la classe d’intégrons de résistance la moins étudiée. Très peu de travaux s’intéressent à leur étude et on dénombre actuellement moins de 10 IR3 entièrement caractérisés. Les objectifs de ce travail de thèse étaient (i) d’effectuer une étude épidémiologique des IR3 en France et au Cameroun et (ii) d’étudier les modalités d’expression de l’intégrase et des cassettes de ces intégrons. Nos travaux ont permis d’isoler puis de décrire 3 nouveaux IR3 présents au sein de bactéries environnementales appartenant aux genres Aeromonas, Acinetobacter et Citrobacter. Les cassettes de ces IR3 codent des résistances aux bétalactamines, aminosides et ammoniums quaternaires. De plus, nous avons caractérisé des IR3 dans 3 souches de Delftia spp. (2 D.acidovorans et 1 D. tsuruhatensis) isolées en Afrique ; les cassettes de ces intégrons ne codent pas de résistance aux antibiotiques. L’axe plus fondamental de ce travail de thèse a permis de montrer que le PintI3(1) est le promoteur impliqué dans l’expression du gène intI3. De plus, nous avons montré que les variants du promoteur Pc, ainsi que les variants du promoteur PintI3(1) sont fonctionnels et de force différente. Il ressort de nos travaux que l’environnement constituerait un réservoir d’intégrons de classe 3 et que ces supports génétiques pourraient jouer un rôle important dans la dissémination de la résistance au sein de cet écosystème.
- Published
- 2016
47. Intégrons de classe 3 : aspects mécanistiques et épidémiologiques
- Author
-
Simo Tchuinte, Pierrette Landrie, STAR, ABES, Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT), CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Université de Limoges, Marie-Cécile Ploy, Antoinette Brigitte Chikaha Tchouya-Ngandjio, and Olivier Barraud
- Subjects
Resistance aux antibiotiques ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,Class 3 integrons ,Antibiotic resistance ,Effluents ,Intégrons de classe 3 ,Delftia ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
Integrons are bacterial genetic elements able to capture and express genes embedded within gene cassettes. They are widely described among Gram-negative bacteria and generally confer a multidrug resistance phenotype. Resistance integrons (RI) play an important role in the acquisition of antibiotic resistance. There are 3 main classes of RI. Class 3 RI has been poorly studied class with less than ten fully IR3 characterized. Objectives of this thesis were (i) to conduct an epidemiological study of class 3 RI in France and Cameroon and (ii) to better understand the modes of expression of the integrase and cassettes of IR3. We described 3 new class 3 RI isolated from environmental bacteria belonging to genus Aeromonas, Acinetobacter and Citrobacter. Gene cassettes encoded resistance to betalactams, aminoglycosides and quaternary ammonium compounds. We also described IR3 from three Delftia strains (2 D.acidovorans and 1 D.tsuruhatensis) in Africa containing cassettes that do not encode antibiotic resistance. The fundamental part of the work showed that the PintI3(1) promoter is involved in the expression of the intI3 gene. Furthermore, we demonstrated that variants of the Pc promoter and variants of the PintI3(1) promoter are functional with different strengths. These results showed that the environment may constitute a reservoir of class 3 integrons and that these genetic elements could play an important role in the spread of the resistance in this ecosystem., Les intégrons sont des supports génétiques bactériens de capture, d’expression et de dissémination de gènes de résistance aux antibiotiques sous forme de cassettes. Ils sont majoritairement décrits chez les bactéries à Gram négatif chez qui ils confèrent généralement un phénotype de multirésistance. Les intégrons de résistance (IR) jouent un rôle majeur dans l’acquisition de la résistance dans le monde bactérien. Il existe 3 principales classes d’IR ; les IR de classe 1, les IR de classe 2 et les IR de classe 3 (IR3). Contrairement aux 2 premières classes, les IR3 représentent la classe d’intégrons de résistance la moins étudiée. Très peu de travaux s’intéressent à leur étude et on dénombre actuellement moins de 10 IR3 entièrement caractérisés. Les objectifs de ce travail de thèse étaient (i) d’effectuer une étude épidémiologique des IR3 en France et au Cameroun et (ii) d’étudier les modalités d’expression de l’intégrase et des cassettes de ces intégrons. Nos travaux ont permis d’isoler puis de décrire 3 nouveaux IR3 présents au sein de bactéries environnementales appartenant aux genres Aeromonas, Acinetobacter et Citrobacter. Les cassettes de ces IR3 codent des résistances aux bétalactamines, aminosides et ammoniums quaternaires. De plus, nous avons caractérisé des IR3 dans 3 souches de Delftia spp. (2 D.acidovorans et 1 D. tsuruhatensis) isolées en Afrique ; les cassettes de ces intégrons ne codent pas de résistance aux antibiotiques. L’axe plus fondamental de ce travail de thèse a permis de montrer que le PintI3(1) est le promoteur impliqué dans l’expression du gène intI3. De plus, nous avons montré que les variants du promoteur Pc, ainsi que les variants du promoteur PintI3(1) sont fonctionnels et de force différente. Il ressort de nos travaux que l’environnement constituerait un réservoir d’intégrons de classe 3 et que ces supports génétiques pourraient jouer un rôle important dans la dissémination de la résistance au sein de cet écosystème.
- Published
- 2016
48. Antimicrobial resistance and molecular characterization of Vibrio cholerae O1 during the 2004 and 2005 outbreak of cholera in Cameroon.
- Author
-
Ngandjio A, Tejiokem M, Wouafo M, Ndome I, Yonga M, Guenole A, Lemee L, Quilici ML, and Fonkoua MC
- Subjects
- Cameroon epidemiology, Cholera epidemiology, Colony Count, Microbial, Disease Outbreaks, Electrophoresis, Gel, Pulsed-Field, Feces microbiology, Food Microbiology, Humans, Microbial Sensitivity Tests, Polymerase Chain Reaction, Ribotyping, Vibrio cholerae O1 genetics, Vibrio cholerae O1 isolation & purification, Anti-Bacterial Agents pharmacology, Cholera microbiology, DNA, Bacterial analysis, Drug Resistance, Multiple, Bacterial, Vibrio cholerae O1 drug effects
- Abstract
There was an outbreak of cholera in Cameroon during 2004 and 2005; the epidemic began in Douala in January 2004 and spread throughout the south of the country. The World Health Organization (WHO) reported 8005 cases in 2004 and 2847 cases in 2005. Five hundred eighty-nine stool samples were received in the Pasteur Centre of Cameroon and 352 were microbiologically confirmed to be positive for Vibrio cholerae O1. Isolated strains were tested for their antimicrobial susceptibilities. All the strains were multidrug resistant and predominantly showed a common resistance pattern at the beginning of the outbreak. Tetracycline, recommended by the WHO for treating cholera in adults, was effective against all the strains tested. Cotrimoxazole (trimethoprim/sulfamethoxazole), previously a first-line treatment in children, was ineffective in vitro for all the clinical isolates and was quickly replaced by amoxicillin. Ampicillin resistance emerged at the end of 2004 and was the leading resistance pattern observed in the second half of 2005. This therefore represented the second major resistance pattern. These two major resistance profiles were not associated with patient characteristics (sex and age) or to the geographic origin of strains. However, there was a highly significant relationship between resistance patterns and the year of isolation (p < 0.001). The strains possessed genes ctxA and ctxB encoding the two cholera toxin subunits and were very closely related, irrespective of their antimicrobial resistance patterns. They were not differentiated by molecular typing methods and gave similar ribotyping and pulsed-field gel electrophoresis patterns.
- Published
- 2009
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.