1. Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.
- Author
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Crowther C, Mowa B, and Arbuthnot P
- Subjects
- Animals, Cytokines metabolism, Drug Delivery Systems adverse effects, Genes, Reporter genetics, Genomics, Hepatitis B Surface Antigens metabolism, Hepatitis B virus genetics, Hepatitis B virus immunology, Hepatitis B virus physiology, Hepatocytes metabolism, Lac Operon genetics, Liver cytology, Liver physiology, Liver virology, Mice, MicroRNAs genetics, RNA Interference, beta-Galactosidase metabolism, Adenoviridae genetics, Drug Delivery Systems methods, Genetic Vectors genetics, Genetic Vectors metabolism, Liver metabolism, MicroRNAs metabolism
- Abstract
The potential of RNA interference (RNAi)-based gene therapy has been demonstrated in many studies. However, clinical application of this technology has been hampered by a paucity of efficient and safe methods of delivering the RNAi activators. Prolonged transgene expression and improved safety of helper-dependent adenoviral vectors (HD AdVs) makes them well suited to delivery of engineered artificial intermediates of the RNAi pathway. Also, AdVs' natural hepatotropism makes them potentially useful for liver-targeted gene delivery. HD AdVs may be used for efficient delivery of cassettes encoding short hairpin RNAs and artificial primary microRNAs to the mouse liver. Methods for the characterization of HD AdV-mediated delivery of hepatitis B virus-targeting RNAi activators are described here.
- Published
- 2016
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