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Your search keyword '"Mariaconcetta Reale"' showing total 2 results
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2 results on '"Mariaconcetta Reale"'

1. Prevalence of Antibiotic Resistance Over Time in a Third-Level University Hospital

Author

Vincenzo Scaglione, Mariaconcetta Reale, Chiara Davoli, Maria Mazzitelli, Francesca Serapide, Rosaria Lionello, Valentina La Gamba, Paolo Fusco, Andrea Bruni, Daniela Procopio, Eugenio Garofalo, Federico Longhini, Nadia Marascio, Cinzia Peronace, Aida Giancotti, Luigia Gallo, Giovanni Matera, Maria Carla Liberto, Bruno Mario Cesana, Chiara Costa, Enrico Maria Trecarichi, Angela Quirino, and Carlo Torti

Subjects
Acinetobacter baumannii, Hospitals, University, Microbiology (medical), Pharmacology, Klebsiella pneumoniae, Drug Resistance, Multiple, Bacterial, Pseudomonas aeruginosa, Immunology, Prevalence, Humans, Microbial Sensitivity Tests, Microbiology, Anti-Bacterial Agents
Abstract

This study evaluated the spread and possible changes in resistance patterns of ESKAPE bacteria to first-choice antibiotics from 2015 to 2019 at a third-level university hospital after persuasive stewardship measures were implemented. Isolates were divided into three groups (group 1, low drug-resistant; group 2, multidrug/extremely drug-resistant; and group 3, pan-resistant bacteria) and a

Published
2022
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2. Real-life 3D therapy failure: Analysis of NS5A 93H RAS plus 108 K polymorphism in complex with ombitasvir by molecular modeling

Author

Sebastiano Di Salvo, Nicola Perrotti, Carmine Talarico, Mariaconcetta Reale, Grazia Pavia, Massimo De Siena, Tiziana Gravina, Anna Artese, Francesca Giancotti, Giorgio Settimo Barreca, Stefano Alcaro, Nadia Marascio, Isabella Romeo, Fernanda Fabiani, Alfredo Focà, Vito Marano, Carlo Torti, and Maria Carla Liberto

Subjects
0301 basic medicine, Male, Models, Molecular, Proline, viruses, Population, Mutation, Missense, Pharmacology, Molecular Dynamics Simulation, Viral Nonstructural Proteins, Antiviral Agents, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Recurrence, Virology, Medicine, Humans, Anilides, Treatment Failure, education, NS5A, NS5B, Aged, education.field_of_study, Dasabuvir, Polymorphism, Genetic, business.industry, virus diseases, Valine, biochemical phenomena, metabolism, and nutrition, Hepatitis C, Chronic, Ombitasvir, 030104 developmental biology, Infectious Diseases, chemistry, Paritaprevir, Phosphoprotein, 030211 gastroenterology & hepatology, Ritonavir, Carbamates, business, medicine.drug
Abstract

We report a real-life 3D therapy failure in a patient treated with ombitasvir (OMV)/paritaprevir/ritonavir and dasabuvir without ribavirin (3D-R). He had therapy failure at week 12 after the end of treatment. We detected resistance-associated substitutions (RASs) plus polymorphisms on NS3, NS5A, and NS5B target regions by population sequencing (15% cut-off) at baseline, at relapse and during follow-up. About this, NS5A RASs generally persist longer than resistances in the other target genes and may impact treatment outcome. Therefore, to evaluate OMV drug-resistance mechanism, we studied the acquired RAS plus polymorphisms on NS5A phosphoprotein by computational studies. OMV showed a higher affinity towards baseline and 93H/108 K mutant structure (follow-up) with respect to 93H/R108 mutant structure (relapse) on phosphoprotein. By Molecular Dynamics simulations (MDs), structural information about the protein stability in presence of OMV were observed. According to our data, molecular modeling approach has proved to be a powerful method to evaluate the impact of these RASs plus specific amino acid (AA) changes on phosphoprotein.

Published
2017

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