1. Accumulation and decay of messenger ribonucleic acid in mouse kidney
- Author
-
Ouellett Aj and Malt Ra
- Subjects
Messenger ribonucleic acid ,Male ,Orotic Acid ,Messenger RNA ,Cytoplasm ,Sucrose ,Time Factors ,Polyadenylation ,RNA ,Kidney ,Biochemistry ,Molecular biology ,chemistry.chemical_compound ,Mice ,chemistry ,RNA, Ribosomal ,Animals ,Eukaryotic Small Ribosomal Subunit ,RNA, Messenger ,Sodium dodecyl sulfate ,Poly A - Abstract
The stability of polyadenylated messenger ribonucleic acid(mRNA) from cytoplasmic structures sedimenting faster than 40S was analyzed in normal mouse kidney. Incorporation of radioactivity into poly(A)-containing and poly(A)-lacking cytoplasmic RNAs separated by oligo(dT)-cellulose chromatography was determined after sedimentation of RNA IN SODIUM DODECYL SULFATE CONTAINING SUCROSE DENSITY GRADIENTS. Radioactivity accumulated in poly(A)-containing RNA during the first 6 h and then decayed exponentially. Beginning 8-12h after administering label, two components were evident in the decay curve of poly(A)-containing RNA; the short-lived component (approximately 57% of newly synthesized molecules) had an apparent half-life of 6h, and the second class (approximately 43% of new mRNA) was more stable, decaying with a 24-h half-life. These studies provide the basis for examining the regulation of mRNA stability during compensatory renal hypertrophy.
- Published
- 1976