1. TNPO3 Is Required for HIV-1 Replication after Nuclear Import but prior to Integration and Binds the HIV-1 Core
- Author
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Patricio Perez, Felipe Diaz-Griffero, Maritza Lienlaf, Abraham L. Brass, Jose Carlos Valle-Casuso, Yang Yang, Francesca Di Nunzio, Nathalie J. Arhel, Natalia Reszka, Department of Microbiology and Immunology, Albert Einstein College of Medicine [New York], Virologie moléculaire et vaccinologie, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Ragon Institute of Massachusetts General Hospital, Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT), and Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Cell Nucleus ,Cytoplasm ,Virus Integration ,Immunology ,Human immunodeficiency virus (HIV) ,Active Transport, Cell Nucleus ,HIV Infections ,MESH: Active Transport, Cell Nucleus ,Biology ,medicine.disease_cause ,Virus Replication ,Microbiology ,gag Gene Products, Human Immunodeficiency Virus ,MESH: HIV-1 ,03 medical and health sciences ,Virology ,medicine ,MESH: Protein Binding ,Humans ,030304 developmental biology ,Cell Nucleus ,0303 health sciences ,MESH: Humans ,030306 microbiology ,MESH: Cytoplasm ,MESH: Virus Replication ,virus diseases ,MESH: HIV Infections ,beta Karyopherins ,MESH: gag Gene Products, Human Immunodeficiency Virus ,Replication (computing) ,3. Good health ,Virus-Cell Interactions ,Core (game theory) ,Insect Science ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,HIV-1 ,Nuclear transport ,MESH: beta Karyopherins ,MESH: Virus Integration ,Protein Binding - Abstract
TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.
- Published
- 2012