1. Green method of synthesizing L-malate from D-glucose via CO2 fixation using an ATP-free in vitro synthetic enzymatic biosystem.
- Author
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Lin Fan, Shangshang Sun, Zhidan Zhang, Yanmei Qin, Ruhdal Jensen, Peter, and Chun You
- Subjects
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MALATE dehydrogenase , *SUSTAINABILITY , *CLIMATE change , *LACTATES , *GLOBAL warming , *CARBON dioxide , *BIOCHEMICAL substrates - Abstract
In conjunction with the pressing global issue of climate change and the associated concern over global warming, increasing interest has emerged in the exploration of carbon dioxide (CO2) as a resource for the generation of a diverse array of products intended to serve societal needs. This study presents the development of an ATP-free and NAD-balanced in vitro synthetic enzymatic biosystem (ivSEB), which comprises only five cascade thermophilic enzymes, designed for the synthesis of L-malate through CO2 fixation powered by the utilization of D-glucose as a substrate. This designed ivSEB yields two moles of L-malate from one mole of D-glucose and two moles of CO2. Through meticulous refinement of reaction conditions and enzyme loading amounts, this ivSEB has demonstrated its capability to produce 6.85 mM of L-malate via CO2 fixation from an initial 5 mM of D-glucose with a molar product yield of 68.5%, and 2.45 mM of L-lactate as a byproduct. In the pursuit of assessing the industrial feasibility of this ivSEB, the study further subjected the system to the utilization of a high concentration (45.70 mM) of D-glucose. Although this endeavor necessitates additional optimization for enhanced efficiency, the present findings herald the emergence of an alternative avenue for the sustainable production of L-malate through CO2 fixation, thus bearing substantial promise for addressing ecological and industrial imperatives. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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