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The development of the embryo sac is an important factor that affects seed setting in rice. Numerous genes associated with embryo sac (ES) development have been identified in plants; however, the function of the DEAD-box RNA helicase family genes is poorly known in rice. Here, we characterized a rice DEAD-box protein, RH52A, which is localized in the nucleus and cytoplasm and highly expressed in the floral organs. The knockout mutant rh52a displayed partial ES sterility, including degeneration of the ES (21%) and the presence of a double-female-gametophyte (DFG) structure (11.8%). The DFG developed from two functional megaspores near the chalazal end in one ovule, and 3.4% of DFGs were able to fertilize via the sac near the micropylar pole in rh52a. RH52A was found to interact with MFS1 and ZIP4, both of which play a role in homologous recombination in rice meiosis. RNA-sequencing identified 234 down-regulated differentially expressed genes associated with reproductive development, including two, MSP1 and HSA1b , required for female germline cell specification. Taken together, our study demonstrates that RH52A is essential for the development of the rice embryo sac and provides cytological details regarding the formation of DFGs. [ABSTRACT FROM AUTHOR]

The topspin forehand is the most frequent and effective shot in top-level table tennis matches. The present study assesses the biomechanical differences in the topspin forehand stroke technique when the incoming ball is in different rotations. Eight national level-1 male table tennis athletes (176.6 ± 4.8 cm, 70.8 ± 6.6 kg, 20.9 ± 2.4 yr) performed two kinds of techniques—topspin forehand strokes against topspin (AT) and against backspin (AB) balls, respectively. The kinematic parameters of their bodies and rackets were calculated. Meanwhile, the force plates analyzed their lower limb moments through inverse dynamics. Paired t-test and non-parametric paired t-test mapping were used to assess the differences between the two conditions. Their center of gravity (COG) vertical distance, elbow flexion, thorax–pelvis flexion, and knee flexion angles had significant differences during the stroke phase (p < 0.05). The moment of the racket-side hip rotation and ankle dorsiflexion showed significant differences during the stroke phase (p < 0.05). The racket velocity, angle, and trajectories showed significant differences at characteristic moments (p < 0.05). This study revealed the different topspin forehand stroke techniques in table tennis, even though the two movements look similar. Compared with AT strokes, the athletes kept a straight elbow and lower center of gravity than they did during the stroke phase of AB strokes. They increased the distance of the racket trajectory and velocity to meet the incoming ball with a backspin, especially in the vertical direction. This detailed information is necessary for beginners to improve the efficiency of their forehand topspin technique, especially in strokes against incoming balls with different rotations. [ABSTRACT FROM AUTHOR]

Background: This study aimed to derive the effects of walking while using a mobile phone on balance perturbation and joint movement among young adults. Methods: Sixteen healthy college students with no history of brain injury were tested. The participants were asked to walk under four different conditions: (1) walking, (2) browsing, (3) dialing, and (4) texting. Indicators related to balance control and lower limb kinematic/kinetic parameters were analyzed using the continuous relative phase and statistical nonparametric mapping methods. Results: Walking while using a mobile phone slowed participants' gait speed and reduced the cadence, stride length, and step length. The posterior tilt angle (0–14%, 57–99%), torque of the hip flexion (0–15%, 30–35%, 75–100%), and angle of the hip flexion (0–28%, 44–100%) decreased significantly. The activation of biceps femoris and gastrocnemius, hip stiffness, and ankle stiffness increased significantly. This impact on gait significantly differed among three dual tasks: texting > browsing > dialing. Conclusion: Che overlap of walking and mobile phone use affects the gait significantly. The "hip–ankle strategy" may result in a "smooth" but slower gait, while this strategy was deliberate and tense. In addition, this adjustment also increases the stiffness of the hip and ankle, increasing the risk of fatigue. Findings regarding this effect may prove that even for young healthy adults, walking with mobile phone use induces measurable adjustment of the motor pattern. These results suggest the importance of simplifying the control of the movement. [ABSTRACT FROM AUTHOR]