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Your search keyword '"Loveless, Bianca"' showing total 10 results
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10 results on '"Loveless, Bianca"'

1. CLIC-01: Manufacture and distribution of non-cryopreserved CAR-T cells for patients with CD19 positive hematologic malignancies

Author

Kekre, Natasha, primary, Hay, Kevin A., additional, Webb, John R., additional, Mallick, Ranjeeta, additional, Balasundaram, Miruna, additional, Sigrist, Mhairi K., additional, Clement, Anne-Marie, additional, Nielsen, Julie S., additional, Quizi, Jennifer, additional, Yung, Eric, additional, Brown, Scott D., additional, Dreolini, Lisa, additional, Waller, Daniel D., additional, Smazynski, Julian, additional, Gierc, Nicole S., additional, Loveless, Bianca C., additional, Clark, Kayla, additional, Dyer, Tyler, additional, Hogg, Richard, additional, McCormick, Leah, additional, Gignac, Michael, additional, Bell, Shanti, additional, Chapman, D. Maria, additional, Bond, David, additional, Yong, Siao, additional, Fung, Rachel, additional, Lockyer, Heather M., additional, Hodgson, Victoria, additional, Murphy, Catherine, additional, Subramanian, Ana, additional, Wiebe, Evelyn, additional, Yoganathan, Piriya, additional, Medynski, Liana, additional, Vaillan, Dominique C., additional, Black, Alice, additional, McDiarmid, Sheryl, additional, Kennah, Michael, additional, Hamelin, Linda, additional, Song, Kevin, additional, Narayanan, Sujaatha, additional, Rodrigo, Judith A., additional, Dupont, Stefany, additional, Hawrysh, Terry, additional, Presseau, Justin, additional, Thavorn, Kednapa, additional, Lalu, Manoj M., additional, Fergusson, Dean A., additional, Bell, John C., additional, Atkins, Harold, additional, Nelson, Brad H., additional, and Holt, Robert A., additional

Published
2022
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5. Structural Characterization and Epitope Mapping of the Glutamic Acid/Alanine-rich Protein from Trypanosoma congolense: DEFINING ASSEMBLY ON THE PARASITE CELL SURFACE*♦

Author

Loveless, Bianca C., Mason, Jeremy W., Sakurai, Tatsuya, Inoue, Noboru, Razavi, Morteza, Pearson, Terry W., and Boulanger, Martin J.

Subjects
Models, Molecular, Structure-Activity Relationship, Tsetse Flies, Trypanosoma congolense, parasitic diseases, Protozoan Proteins, Animals, Molecular Bases of Disease, Cattle, Crystallography, X-Ray, Epitope Mapping, Protein Structure, Secondary
Abstract

Trypanosoma congolense is an African trypanosome that causes serious disease in cattle in Sub-Saharan Africa. The four major life cycle stages of T. congolense can be grown in vitro, which has led to the identification of several cell-surface molecules expressed on the parasite during its transit through the tsetse vector. One of these, glutamic acid/alanine-rich protein (GARP), is the first expressed on procyclic forms in the tsetse midgut and is of particular interest because it replaces the major surface coat molecule of bloodstream forms, the variant surface glycoprotein (VSG) that protects the parasite membrane, and is involved in antigenic variation. Unlike VSG, however, the function of GARP is not known, which necessarily limits our understanding of parasite survival in the tsetse. Toward establishing the function of GARP, we report its three-dimensional structure solved by iodide phasing to a resolution of 1.65 Å. An extended helical bundle structure displays an unexpected and significant degree of homology to the core structure of VSG, the only other major surface molecule of trypanosomes to be structurally characterized. Immunofluorescence microscopy and immunoaffinity-tandem mass spectrometry were used in conjunction with monoclonal antibodies to map both non-surface-disposed and surface epitopes. Collectively, these studies enabled us to derive a model describing the orientation and assembly of GARP on the surface of trypanosomes. The data presented here suggest the possible structure-function relationships involved in replacement of the bloodstream form VSG by GARP as trypanosomes differentiate in the tsetse vector after a blood meal.

Published
2011

6. Structural and Biochemical Characterization of Plasmodium falciparum 12 (Pf12) Reveals a Unique Interdomain Organization and the Potential for an Antiparallel Arrangement with Pf41

Author

Tonkin, Michelle L., primary, Arredondo, Silvia A., additional, Loveless, Bianca C., additional, Serpa, Jason J., additional, Makepeace, Karl A.T., additional, Sundar, Natarajan, additional, Petrotchenko, Evgeniy V., additional, Miller, Louis H., additional, Grigg, Michael E., additional, and Boulanger, Martin J., additional

Published
2013
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10. Purification, crystallization and X-ray diffraction analysis of Trypanosoma congolense insect-stage surface antigen (TcCISSA).

Author

Tonkin, Michelle L., Workman, Sean D., Eyford, Brett A., Loveless, Bianca C., Fudge, Jessica L., Pearson, Terry W., and Boulanger, Martin J.

Subjects
TRYPANOSOMA, CRYSTALLIZATION, X-ray diffraction, CELL surface antigens, RECOMBINANT DNA
Abstract

Trypanosoma congolense is a major contributor to the vast socioeconomic devastation in sub-Saharan Africa caused by animal African trypanosomiasis. These protozoan parasites are transmitted between mammalian hosts by tsetse-fly vectors. A lack of understanding of the molecular basis of tsetse-trypanosome interactions stands as a barrier to the development of improved control strategies. Recently, a stage-specific T. congolense protein, T. congolense insect-stage surface antigen (TcCISSA), was identified that shows considerable sequence identity (>60%) to a previously identified T. brucei insect-stage surface molecule that plays a role in the maturation of infections. TcCISSA has multiple di-amino-acid and tri-amino-acid repeats in its extracellular domain, making it an especially interesting structure-function target. The predicted mature extracellular domain of TcCISSA was produced by recombinant DNA techniques, purified from Escherichia coli, crystallized and subjected to X-ray diffraction analysis; the data were processed to 2.7 Å resolution. [ABSTRACT FROM AUTHOR]

Published
2012
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