7 results on '"Linn, Doug"'
Search Results
2. The Discovery of Two Novel Classes of 5,5-Bicyclic Nucleoside-Derived PRMT5 Inhibitors for the Treatment of Cancer
- Author
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Quiroz, Ryan V., primary, Reutershan, Michael H., additional, Schneider, Sebastian E., additional, Sloman, David, additional, Lacey, Brian M., additional, Swalm, Brooke M., additional, Yeung, Charles S., additional, Gibeau, Craig, additional, Spellman, Daniel S., additional, Rankic, Danica A., additional, Chen, Dapeng, additional, Witter, David, additional, Linn, Doug, additional, Munsell, Erik, additional, Feng, Guo, additional, Xu, Haiyan, additional, Hughes, Jonathan M. E., additional, Lim, Jongwon, additional, Saurí, Josep, additional, Geddes, Kristin, additional, Wan, Murray, additional, Mansueto, My Sam, additional, Follmer, Nicole E., additional, Fier, Patrick S., additional, Siliphaivanh, Phieng, additional, Daublain, Pierre, additional, Palte, Rachel L., additional, Hayes, Robert P., additional, Lee, Sandra, additional, Kawamura, Shuhei, additional, Silverman, Steven, additional, Sanyal, Sulagna, additional, Henderson, Timothy J., additional, Ye, Yingchun, additional, Gao, Yuanwei, additional, Nicholson, Benjamin, additional, and Machacek, Michelle R., additional
- Published
- 2021
- Full Text
- View/download PDF
3. Combination of EP4 antagonist MF-766 and anti-PD-1 promotes anti-tumor efficacy by modulating both lymphocytes and myeloid cells
- Author
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Wang, Yun, primary, Cui, Long, additional, Georgiev, Peter, additional, Singh, Latika, additional, Zheng, Yanyan, additional, Yu, Ying, additional, Grein, Jeff, additional, Zhang, Chunsheng, additional, Muise, Eric S., additional, Sloman, David L., additional, Ferguson, Heidi, additional, Yu, Hongshi, additional, Pierre, Cristina St., additional, Dakle, Pranal J, additional, Pucci, Vincenzo, additional, Baker, James, additional, Loboda, Andrey, additional, Linn, Doug, additional, Brynczka, Christopher, additional, Wilson, Doug, additional, Haines, Brian B, additional, Long, Brian, additional, Wnek, Richard, additional, Sadekova, Svetlana, additional, Rosenzweig, Michael, additional, Haidle, Andrew, additional, Han, Yongxin, additional, and Ranganath, Sheila H., additional
- Published
- 2021
- Full Text
- View/download PDF
4. Combination of EP4 antagonist MF-766 and anti-PD-1 promotes anti-tumor efficacy by modulating both lymphocytes and myeloid cells.
- Author
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Yun Wang, Long Cui, Georgiev, Peter, Singh, Latika, Yanyan Zheng, Ying Yu, Grein, Jeff, Chunsheng Zhang, Muise, Eric S., Sloman, David L., Ferguson, Heidi, Hongshi Yu, St. Pierre, Cristina, Dakle, Pranal J., Pucci, Vincenzo, Baker, James, Loboda, Andrey, Linn, Doug, Brynczka, Christopher, and Wilson, Doug
- Subjects
MYELOID cells ,MYELOID-derived suppressor cells ,PEMBROLIZUMAB ,KILLER cells ,LYMPHOCYTES ,PROSTAGLANDIN receptors ,TUMOR necrosis factors - Abstract
Prostaglandin E
2 (PGE2 ), an arachidonic acid pathway metabolite produced by cyclooxygenase (COX)-1/2, has been shown to impair anti-tumor immunity through engagement with one or more E-type prostanoid receptors (EP1-4 ). Specific targeting of EP receptors, as opposed to COX-1/2 inhibition, has been proposed to achieve preferential antagonism of PGE2 -mediated immune suppression. Here we describe the anti-tumor activity of MF-766, a potent and highly selective small-molecule inhibitor of the EP4 receptor. EP4 inhibition by MF-766 synergistically improved the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in CT26 and EMT6 syngeneic tumor mouse models. Multiparameter flow cytometry analysis revealed that treatment with MF-766 promoted the infiltration of CD8+ T cells, natural killer (NK) cells and conventional dendritic cells (cDCs), induced M1-like macrophage reprogramming, and reduced granulocytic myeloid-derived suppressor cells (MDSC) in the tumor microenvironment (TME). In vitro experiments demonstrated that MF-766 restored PGE2 -mediated inhibition of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α production in THP-1 cells and human blood, and PGE2 -mediated inhibition of interleukin (IL)-2-induced interferon (IFN)-γ production in human NK cells. MF-766 reversed the inhibition of IFN-γ in CD8+ T-cells by PGE2 and impaired suppression of CD8+ T-cells induced by myeloid-derived suppressor cells (MDSC)/PGE2 . In translational studies using primary human tumors, MF-766 enhanced anti-CD3-stimulated IFN-γ, IL-2, and TNF-α production in primary histoculture and synergized with pembrolizumab in a PGE2 high TME. Our studies demonstrate that the combination of EP4 blockade with anti-PD-1 therapy enhances antitumor activity by differentially modulating myeloid cell, NK cell, cDC and T-cell infiltration profiles. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
5. Combination of EP4antagonist MF-766 and anti-PD-1 promotes anti-tumor efficacy by modulating both lymphocytes and myeloid cells
- Author
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Wang, Yun, Cui, Long, Georgiev, Peter, Singh, Latika, Zheng, Yanyan, Yu, Ying, Grein, Jeff, Zhang, Chunsheng, Muise, Eric S., Sloman, David L., Ferguson, Heidi, Yu, Hongshi, Pierre, Cristina St., Dakle, Pranal J, Pucci, Vincenzo, Baker, James, Loboda, Andrey, Linn, Doug, Brynczka, Christopher, Wilson, Doug, Haines, Brian B, Long, Brian, Wnek, Richard, Sadekova, Svetlana, Rosenzweig, Michael, Haidle, Andrew, Han, Yongxin, and Ranganath, Sheila H.
- Abstract
ABSTRACTProstaglandin E2(PGE2), an arachidonic acid pathway metabolite produced by cyclooxygenase (COX)-1/2, has been shown to impair anti-tumor immunity through engagement with one or more E-type prostanoid receptors (EP1-4). Specific targeting of EP receptors, as opposed to COX-1/2 inhibition, has been proposed to achieve preferential antagonism of PGE2–mediated immune suppression. Here we describe the anti-tumor activity of MF-766, a potent and highly selective small-molecule inhibitor of the EP4receptor. EP4inhibition by MF-766 synergistically improved the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in CT26 and EMT6 syngeneic tumor mouse models. Multiparameter flow cytometry analysis revealed that treatment with MF-766 promoted the infiltration of CD8+T cells, natural killer (NK) cells and conventional dendritic cells (cDCs), induced M1-like macrophage reprogramming, and reduced granulocytic myeloid-derived suppressor cells (MDSC) in the tumor microenvironment (TME). In vitroexperiments demonstrated that MF-766 restored PGE2-mediated inhibition of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α production in THP-1 cells and human blood, and PGE2-mediated inhibition of interleukin (IL)-2-induced interferon (IFN)-γ production in human NK cells. MF-766 reversed the inhibition of IFN-γ in CD8+T-cells by PGE2and impaired suppression of CD8+T-cells induced by myeloid-derived suppressor cells (MDSC)/PGE2.In translational studies using primary human tumors, MF-766 enhanced anti-CD3-stimulated IFN-γ, IL-2, and TNF-α production in primary histoculture and synergized with pembrolizumab in a PGE2high TME. Our studies demonstrate that the combination of EP4blockade with anti-PD-1 therapy enhances antitumor activity by differentially modulating myeloid cell, NK cell, cDC and T-cell infiltration profiles.
- Published
- 2021
- Full Text
- View/download PDF
6. Activation of Hepatocyte Growth Factor/MET Signaling as a Mechanism of Acquired Resistance to a Novel YAP1/TEAD Small Molecule Inhibitor.
- Author
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Moure CJ, Vara B, Cheng MM, Sondey C, Muise E, Park E, Vela Ramirez JE, Su D, D'Souza S, Yan Q, Yeung CS, Zhang M, Mansueto MS, Linn D, Buchanan M, Foti R, DiMauro E, Long B, Simov V, and Barry ER
- Subjects
- Humans, Animals, Mice, Cell Line, Tumor, Xenograft Model Antitumor Assays, Small Molecule Libraries pharmacology, TEA Domain Transcription Factors, Cell Proliferation drug effects, Hepatocyte Growth Factor metabolism, Transcription Factors metabolism, Transcription Factors antagonists & inhibitors, Proto-Oncogene Proteins c-met antagonists & inhibitors, Proto-Oncogene Proteins c-met metabolism, Signal Transduction drug effects, Adaptor Proteins, Signal Transducing metabolism, Adaptor Proteins, Signal Transducing antagonists & inhibitors, YAP-Signaling Proteins, Drug Resistance, Neoplasm drug effects
- Abstract
Many tumor types harbor alterations in the Hippo pathway, including mesothelioma, where a high percentage of cases are considered YAP1/TEAD dependent. Identification of autopalmitoylation sites in the hydrophobic palmitate pocket of TEADs, which may be necessary for YAP1 protein interactions, has enabled modern drug discovery platforms to generate compounds that allosterically inhibit YAP1/TEAD complex formation and transcriptional activity. We report the discovery and characterization of a novel YAP1/TEAD inhibitor MRK-A from an aryl ether chemical series demonstrating potent and specific inhibition of YAP1/TEAD activity. In vivo, MRK-A showed a favorable tolerability profile in mice and demonstrated pharmacokinetics suitable for twice daily oral dosing in preclinical efficacy studies. Importantly, monotherapeutic targeting of YAP1/TEAD in preclinical models generated regressions in a mesothelioma CDX model; however, rapid resistance to therapy was observed. RNA-sequencing of resistant tumors revealed mRNA expression changes correlated with the resistance state and a marked increase of hepatocyte growth factor (HGF) expression. In vitro, exogenous HGF was able to fully rescue cytostasis induced by MRK-A in mesothelioma cell lines. In addition, co-administration of small molecule inhibitors of the MET receptor tyrosine kinase suppressed the resistance generating effect of HGF on MRK-A induced growth inhibition. In this work, we report the structure and characterization of MRK-A, demonstrating potent and specific inhibition of YAP1/TAZ-TEAD-mediated transcriptional responses, with potential implications for treating malignancies driven by altered Hippo signaling, including factors resulting in acquired drug resistance., (©2024 American Association for Cancer Research.)
- Published
- 2024
- Full Text
- View/download PDF
7. Combination of EP 4 antagonist MF-766 and anti-PD-1 promotes anti-tumor efficacy by modulating both lymphocytes and myeloid cells.
- Author
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Wang Y, Cui L, Georgiev P, Singh L, Zheng Y, Yu Y, Grein J, Zhang C, Muise ES, Sloman DL, Ferguson H, Yu H, Pierre CS, Dakle PJ, Pucci V, Baker J, Loboda A, Linn D, Brynczka C, Wilson D, Haines BB, Long B, Wnek R, Sadekova S, Rosenzweig M, Haidle A, Han Y, and Ranganath SH
- Subjects
- Animals, Cyclooxygenase 2, Dinoprostone, Macrophages, Mice, CD8-Positive T-Lymphocytes, Receptors, Prostaglandin E, EP4 Subtype
- Abstract
Prostaglandin E
2 (PGE2 ), an arachidonic acid pathway metabolite produced by cyclooxygenase (COX)-1/2, has been shown to impair anti-tumor immunity through engagement with one or more E-type prostanoid receptors (EP1-4 ). Specific targeting of EP receptors, as opposed to COX-1/2 inhibition, has been proposed to achieve preferential antagonism of PGE2 -mediated immune suppression. Here we describe the anti-tumor activity of MF-766, a potent and highly selective small-molecule inhibitor of the EP4 receptor. EP4 inhibition by MF-766 synergistically improved the efficacy of anti-programmed cell death protein 1 (PD-1) therapy in CT26 and EMT6 syngeneic tumor mouse models. Multiparameter flow cytometry analysis revealed that treatment with MF-766 promoted the infiltration of CD8+ T cells, natural killer (NK) cells and conventional dendritic cells (cDCs), induced M1-like macrophage reprogramming, and reduced granulocytic myeloid-derived suppressor cells (MDSC) in the tumor microenvironment (TME). In vitro experiments demonstrated that MF-766 restored PGE2 -mediated inhibition of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α production in THP-1 cells and human blood, and PGE2 -mediated inhibition of interleukin (IL)-2-induced interferon (IFN)-γ production in human NK cells. MF-766 reversed the inhibition of IFN-γ in CD8+ T-cells by PGE2 and impaired suppression of CD8+ T-cells induced by myeloid-derived suppressor cells (MDSC)/PGE2. In translational studies using primary human tumors, MF-766 enhanced anti-CD3-stimulated IFN-γ, IL-2, and TNF-α production in primary histoculture and synergized with pembrolizumab in a PGE2 high TME. Our studies demonstrate that the combination of EP4 blockade with anti-PD-1 therapy enhances antitumor activity by differentially modulating myeloid cell, NK cell, cDC and T-cell infiltration profiles., (© 2021 Merck and Co. Published with license by Taylor & Francis Group, LLC.)- Published
- 2021
- Full Text
- View/download PDF
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