14 results on '"Laurito S"'
Search Results
2. Epigenetic regulation of ID4 in the determination of the BRCAness phenotype in breast cancer
- Author
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Branham, M. T., primary, Campoy, E., additional, Laurito, S., additional, Branham, R., additional, Urrutia, G., additional, Orozco, J., additional, Gago, F., additional, Urrutia, R., additional, and Roqué, M., additional
- Published
- 2015
- Full Text
- View/download PDF
3. Methylation profile of triple-negative breast carcinomas
- Author
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Branham, M T, primary, Marzese, D M, additional, Laurito, S R, additional, Gago, F E, additional, Orozco, J I, additional, Tello, O M, additional, Vargas-Roig, L M, additional, and Roqué, M, additional
- Published
- 2012
- Full Text
- View/download PDF
4. Neotropical xenarthrans: a data set of occurrence of xenarthran species in the Neotropics
- Author
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Guilherme Braga Ferreira, Javier de la Maza, Sandra M. C. Cavalcanti, Samir Gonçalves Rolim, Diana Letícia Kruger Pacheco Carvalho, Juan L. Peña-Mondragón, Jessica Castro-Prieto, Maria Luisa S. P. Jorge, Carlos De Angelo, Micheli Ribeiro Luiz, Daniel H. Thornton, Jesús A. Iglesias, Arthur Francisco Araújo Fernandes, Jonas Sponchiado, Juliani Bruna Zanoni, Lucas Lacerda Toth Quintilham, Thadeu Sobral-Souza, Daniel da Silva Ferraz, Rayana Diniz da Silva, Agustin Manuel Abba, María Eugenia Iezzi, Andreas Kindel, Flávia Regina Miranda, Gabriel Ivan Boaglio, Pamella Gusmão de Goés Brennand, Maurício Eduardo Graipel, Paula Alves Condé, Paulina Arroyo-Gerala, Rogério Cunha de Paula, Sebastián Andrés Costa, Natasha Moraes de Albuquerque, Teresa Cristina Anacleto, Erich Fischer, Adriano Garcia Chiarello, Fernando M. Contreras-Moreno, Roan McNab, André Luis Regolin, Zoila Vega Guarderas, Francesca Belem Lopes Palmeira, Rodrigo de Almeida Nobre, Marcia Maria de Assis Jardim, Diego Queirolo, Erika Castro, Luciano Carramaschi de Alagão Querido, Freddy Pérez-Garduza, Gabriel Favero Massocato, Karine Galisteo Diemer Lopes, Beatriz Fernandes Lima Luciano, Bruno Rodrigo de Albuquerque França, Enrique González, Giordano Ciocheti, Agustin Paviolo, Eleonore Z. F. Setz, Victor Gasperotto Krepschi, Felipe Martello, Juan Pablo Arrabal, Paulo de Tarso Zuquim Antas, Daiane Buscariol, Frederico Gemesio Lemos, Joana Zorzal Nodari, Cristiano Trapé Trinca, Fernanda Santos, Valéria da Cunha Tavares, Luis Renato Rezende Bernardo, Maria Angélica Barbosa Beccato, Juliana F. Ribeiro, Marina Ochoa Favarini, Alexine Keuroghlian, Cesar Rojano, Márcio Leite de Oliveira, Laura K. Honda, Lilian Elaine Rampim, Paloma Marques Santos, Nicolás Cantero, Helena Alves do Prado, Miriam Lucia Lages Perilli, Whaldener Endo, Diego Varela, Mauricio M. Núñez-Regueiro, Ernesto B.Viveiros de Castro, Fábio de Barros, Sebastián A. Ballari, Andreia Magro Moraes, Scarlat Dalva Ferreira, Lerrane de Fatima Cunha, William James Loughry, Ana Cecilia Ochoa, Alexandra Cravino Mol, Milton José de Paula, Igor Pfeifer Coelho, Samuel Eurich Betkowski, Erika de la Peña-Cuéllar, Milene Alves-Eigenheer, Evelyn Beatriz Brítez, Benoit de Thoisy, María Alicia de la Colina, Fabiana Lopes Rocha, Bibiana Gómez-Valencia, Cecília Licarião Luna, Hugo Fernandes-Ferreira, Gustavo Gonsioroski, Jeffrey J. Thompson, Deborah Faria, Izar Aximoff, Katyucha Von Kossel de Andrade Silva, Pierre-Cyril Renaud, Raone Beltrão-Mendes, Carlos Henrique Salvador, Alexandre Casagrande Faustino, Pedro Volkmer de Castilho, Paula Koeler Lira, Natalia Fraguas Versiani, Ricardo Sampaio, Santiago Carvalho, Marina Zanin, Geruza Leal Melo, Anne Karoline de Oliveira, Paulo Afonso Hartmann, Leonardo Carreira Trevelin, Marianela Velilla, Ana Raíssa Cunha Costa, Luiz Flamarion B. Oliveira, Patrício Adriano da Rocha, Carla Danielle de Melo-Soares, Dênis A. Sana, Danielle de Oliveira Moreira, Nivaldo Peroni, Carolina Depolito Melo, Marina Furlan Giubbina, José Julio de Toledo, Fredy Ramírez Pinto, Julio Chacón Pacheco, Javier Hinojosa, Pablo Gerardo Fernández Santiago, Maximiliano Augusto Benedetti, Vinícius Santana Orsini, Patrícia Gonçalves Guedes, Elisandra de Almeida Chiquito, Fabiane Girardi, Douglas de Matos Dias, Layla Reis de Andrade, Soledad de Bustos, Maria João Ramos Pereira, Wellington Hannibal, Mariana Bueno Landis, Juliano André Bogoni, Gindomar Gomes Santana, Eloisa Neves Mendonça, Miguel Coutinho Moretta Monteiro, Andre Monnerat Lanna, Isadora Beraldi Esperandio, Francys E. da Veiga da Costa, Sérgio Lucena Mendes, Wesley Dáttilo, Juan M. Campos Krauer, Sebastián Cirignoli, Fernando Gonçalves, Caryne Braga, Helena Godoy Bergallo, Ariane Teixeira Bertoldi, J. Antonio de la Torre, Luciana Souza Araújo, Paulo Marinho, Carla Cristina Gestich, Magnus Machado Severo, Ludmilla Oliveira Ribeiro, Juliane Pereira-Ribeiro, Jairo José Zocche, Mariana B. Nagy-Reis, Fernanda Zimmermann Teixeira, Fábio D. Mazim, Fernando Ferreira de Pinho, Leandro Perez Godoy, André Valle Nunes, Mozart C. Freitas-Junior, André Luiz Ferreira da Silva, Bruno Leles, Flávio Kulaif Ubaid, Fernando Pedroni, Vilmar Picinatto Filho, Sofia Marques Silva, Allison L. Devlin, Denise Lidoro de Mattia, William Douglas de Carvalho, Juan A. Martínez-Lanfranco, Marcela Alvares Oliveira, Bruna Silva Santos, Jorge Ferreira Lima Neto, Fernando Lima, Emiliano Guijosa-Guadarrama, Amadeo Sánchez, Juan I. Reppucci, Sixto Fernández Ramirez, Simonne Chinem, Ana Yoko Ykeuti Meiga, Vinicius A. G. Bastazini, Omar Correia Neto, Gabriel Lima Aguiar, Camila Cantagallo, Luiz H. Varzinczak, Italo Mourthe, Yamil Edgardo Di Blanco, Lydia Möcklinghoff, Bruna Gomes Oliveira, Stefani Gabrieli Age, Gabriel Preuss, Pryscilla Moura Lombardi, José Maurício Barbanti Duarte, Nicholas Gengler, Paul François Colas-Rosas, Paula Gonzalez Ciccia, Fernanda Guedes da Silva, Claudia Zukeran Kanda, Marcelo Hideki Yamane, Marina Lima da Silva, Gustavo Alvez da Costa Toledo, Cintia Gisele Tellaeche, Guilherme Cavicchioli, Larissa Oliveira Gonçalves, Juliana Silveira dos Santos, Alessandra Bertassoni, Newton Mota Filho, Nila Rássia Costa Gontijo, Maria Augusta Andrade da Silva, Guillermo E. Gil, Cyntia Cavalcante Santos, Alexandre Camargo Martensen, Anelise Montanarin, Barbara Zimbres, Anna Carolina Figueiredo de Albuquerque, Frédéric Delsuc, Mircea Mihart Hidalgo, Fernando R. Tortato, Breno Campelo Lima, Ana Cristina Mendes de Oliveira, Rodolfo Assis Magalhães, Eduardo Marques Santos, Ezequiel Pedó, Danianderson Rodrigues Carvalho, Marcelo Cervini, Antonio M. Mangione, Nereyda Falconi, Jose Roberto de Matos, Roberta Montanheiro Paolino, Mauricio Neves Godoi, Rodrigo Costa Araújo, Tayana Godim, Itiberê P. Bernardi, Daniel M. Casali, Alberto Gonzalez Gallina, Flávia P. Tirelli, Carlos Henrique de Freitas, Marcelo Juliano Rabelo Oliveira, Viviana B. Rojas Bonzi, Fernando A. Perini, Catalina Sánchez-Lalinde, Daniela Rodrigues Vasconcellos, Katia Maria Paschoaletto Micchi de Barros Ferraz, Juarez Carlos Brito Pezzuti, Julia Camara Assis, João Gabriel Ribeiro Giovanelli, Lucía Martínez Retta, Renata Twardowsky Ramalho Bonikowski, Rubem A.P. Dornas, Igor Kintopp Ribeiro, Alvaro García-Olaechea, Ricardo Sartorello, Camila Clozato Lara, Marcos Adriano Tortato, Clinton N. Jenkins, Anderson Feijó, Andrew J. Noss, Roque Lázaro de Gaspari Júnior, Alberto Yanosky, Gabriela Teixeira Duarte, Yaribeth Bravata de la Cruz, Erica Vanessa Maggiorini, Robson Odeli Espíndola Hack, Marcos de Souza Fialho, Noé U. de la Sancha, Camila Silveira Lima, Ricardo S. Bovendorp, Cláudia Bueno de Campos, Fernando Gaspari, Marcelo de Assumpção Pereira da Silva, Alexandre Reis Percequillo, Mauro Galetti, Manoel dos Santos-Filho, Filipe Pereira Rego Santos, Alexandre Martins Costa Lopes, Lucas Neves Perillo, Cindy M. Hurtado, Paula Akkawi, Lilian Bonjorne, Rony García Anleu, Julia Martinez Pardo, Anamélia de Souza Jesus, Ramon Lima Silva, Kena Ferrari Moreira da Silva, Franco L. Souza, Maria Santina de Castro Morini, Arnaud Leonard Jean Desbiez, Caroline Leuchtenberger, João Alves de Oliveira, Ailin Gatica, Luiza Neves Guimarães, Alan N. Costa, Gustavo Gabirele Gaspari, Colleen McDonough, Marcela Figuerêdo Duarte Moraes, Erick Francisco Aguiar, Átilla Colombo Ferreguetti, Denison José Henz, Adryelle Francisca de Souza Moreira, Eduardo G. Carrano, Cristina Jaques da Cunha, Edson Fiedler de Abreu-Júnior, Mariana Sampaio Xavier, Gabriel Selbach Hofmann, Ana Cristyna Reis Lacerda, Ricardo Corassa Arrais, Viviane Maria Guedes Layme, Paulo Ribeiro, Rodrigo Lima Massara, Francisco Grotta-Neto, Jéssica Caroline de Faria Falcão, Gustavo A. Marás, Milton Cezar Ribeiro, Elizandra de Matos Cardoso, Antonio Rossano Mendes Pontes, Armando Muniz Calouro, Tatiane Campos Trigo, Adriana Bocchiglieri, Bernardo Brandão Niebuhr, Otávio Santi Ribeiro, Wilson Roberto Spironello, Emiliano Esterci Ramalho, Ângela Camila Deffaci, Santiago Escobar, Rodrigo Raúl León Pérez, Akyllan Zoppi Medeiro, Ricardo Miranda Braga, Hugo Cabral, Maíra Benchimol, Sean Keuroghlian-Eaton, Juan Carlos Rudolf, Nina Attias, Felipe Moreli Fantacini, Jardel Brandão Seibert, Laura K. Marsh, Sérgio Bazilio, Laís dos Santos Everton, Fernando Cesar Cascelli de Azevedo, Marcelo Passamani, Liana Mara Mendes de Sena, Mario Henrique Alves, Franciane Almeida da Silva, Vinícius Peron de Oliveira Gasparotto, Karl-L. Schuchmann, Ana Carolina Srbek-Araujo, Emerson M. Vieira, Felipe Pedrosa, Clarissa Alves da Rosa, Ricardo Machado, Júlia Beduschi, Júlia Emi de Faria Oshima, Luiz Gustavo R. Oliveira-Santos, Henrique Villas Boas Concone, Alexandre Vogliotti, Leonardo Henrique da Silva, Débora Regina Yogui, Manuela Vieira dos Santos, Carlos Candia-Gallardo, Rafael Souza Cruz Alves, Marcelo Magioli, Adriano Pereira Paglia, Murillo Prado da Silva, Pablo Rodrigues Gonçalves, Marina Rivero, Jose Luis Cartes, Lívia de Almeida Rodrigues, Mayara Guimarães Beltrão, Felipe Bortolotto Peters, Jéssica Helena Mangueira Dias, Josué Santos Almeida, Robert L. Wallace, Ludmila Hufnagel, Daiane Cristina Carreira, Danielle D. Brown, Rogério Grassetto Teixeira da Cunha, Jorge José Cherem, Helio Secco, Pablo G. Perovic, Daniele Barcelos, Ubiratan Piovezan, Caetano Troncoso Oliveira, Elvira D'Bastiani, André Hirsch, Ana Maria de Oliveira Paschoal, Marina Xavier da Silva, Valeria Towns, Edgar Federico Rivadeneira, Marinêz Isaac Marques, Ronaldo Gonçalves Morato, Roxane Schaub, Marcus Vinícius Vieira, Ednaldo Cândido Rocha, Shirley Seixas Pereira Silva, Ingridi Camboim Franceschi, Rodrigo Ayala, Mariana Guenther, Fernando Carvalho, Paula Modenesi Ferreira, John Polisar, Rafael Reyna Hurtado, Burton K. Lim, Alejandro Jesus, Andressa Gatti, Agnis Cristiane de Souza, Pedro Henrique de Faria Peres, Luziene Conceição de Sousa, Gabriela S Oliveira, Alex Bager, Ana Kellen Nogueira Campelo, Marcell Soares Pinheiro, Carlos Eduardo Lustosa Esbérard, Marília A. S. Barros, Marcos Dums, Gabrielle Beca, Daniella Leal Ramos, Bruno Augusto Torres Parahyba Campos, Flavia Caruso, Marcelo Gordo, Diana Friedeberg, Fernanda Delborgo Abra, Luana Marina de Castro Mendonça, Carlos Benhur Kasper, Silvia Benito Santamaría, Fabio de Oliveira Roque, Cinthya Chiva dos Santos, Guilherme Mourão, José Fernando Moreira Ramírez, Rita de Cassia Bianchi, Mario S. Di Bitetti, Nacho Villar, Fernando C. Passos, Flávio Henrique Guimarães Rodrigues, Herbert Duarte, Sabrina Laurito, João Paulo Pandini Favoretti, Paula Cruz, Olivier Pays, Diego Astúa, Pedro Cordeiro Estrela, Saulo Ramos Lima, Cecília Bueno, Tadeu Gomes de Oliveira, Alessandro Rocha, Tainah Cruz Moreira, Laury Cullen, Lucas Lobo Barbosa, Carlos A. Peres, William Bercê, Sara Cortez, Raissa Danielle Praxedes Grangeiro, Rafael Hoogesteijn, Thiago Bernardes Maccarini, María José Andrade-Núñez, Carlos Eduardo Fragoso, Alex Augusto Abreu Bovo, Lucas Leuzinger, Nilton C. Cáceres, Luiz Henrique Medeiros Borges, Joceleia G. Koenemann, Nielson Pasqualotto, Rugieri Juárez, Graziele Oliveira Batista, Micaela Camino, Kathrin Burs, Andrezza Bellotto Nobre, Elildo A.R. Carvalho, Nathália Fernandes Canassa, Donald P. Eaton, Carlos Rodrigo Brocardo, Bráulio A. Santos, Fernanda Cavalcanti de Azevedo, Nicole da Rosa Oliveira, Thiago Ferreira Rodrigues, Verónica Andrea Quiroga, Bernardo Papi, André Luis Moura Botelho, Hugo Fernando del Castillo Cordero, Rosane Vieira Marques, Hugo Reis Medeiros, Gastón Andrés Fernandez Giné, Natalia Mariana Denkiewicz, Vinicius Rodrigues Tonetti, Rafael de Souza Laurindo, Paula Fabiana Pinheiro, Larissa L. Bailey, Martin Roberto Del Valle Alvarez, Ezequiel Vanderhoeven, Vinicius Alberici, Cynthia Elisa Widmer, Claudia Regina Silva, Leonardo Sartorello, Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Marcos Santos P., Bocchiglieri A., Garcia Chiarello A., Pereira Paglia A., Moreira A., Souza A.C., Abba A.M., Gatica A., Zoppi Medeiro A., Costa A.N., Gonzalez Gallina A., Yanosky A., Jesus A., Bertassoni A., Rocha A., Abreu Bovo A.A., Bager A., Cravino Mol Alexandra, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Ecología y Ciencias Ambientales., Camargo Martensen A., Casagrande Faustino A., Martins Costa Lopes A., Reis Percequillo A., Vogliotti A., Keuroghlian A., Colina M.A., Devlin A., García-Olaechea A., Sánchez A., Srbek-Araujo A.C., Ochoa A.C., Mendes Oliveira A.C., Reis Lacerda A.C., Nogueira Campelo A.N., Oliveira Paschoal A.M., Cunha Costa A.R., Ykeuti Meiga A.Y., Souza Jesus A., Feijó A., Hirsch A., Ferreira da Silva A.F., Moura Botelho A.L., Regolin A.L., Monnerat Lanna A., Valle Nunes A., Kindel A., Magro Moraes A., Gatti A., Noss A., Bellotto Nobre A., Montanarin A., Deffaci A.C., Figueiredo de Albuquerque A.C., Oliveira A.K., Mangione A.M., Rossano Mendes Pontes A., Teixeira Bertoldi A., Muniz Calouro A., Desbiez A., Fernandes A., Colombo Ferreguetti A., Andrade da Silva M.A., Zimbres B., Fernandes Lima Luciano B., Thoisy B., Niebuhr B.B., Papi B., Gómez-Valencia B., Santos B., Campelo Lima B., Gomes Oliveira B., Silva Santos B., Torres Parahyba Campos B.A., Leles B., Albuquerque França B.R., Lim B., Troncoso Oliveira C., Cantagallo C., Clozato Lara C., Silveira Lima C., Gestich C.C., Melo-Soares C.D., Peres C., Benhur Kasper C., Candia-Gallardo C., Angelo C., Fragoso C.E., Freitas C.H., Salvador C.H., Brocardo C.R., Depolito Melo C., Leuchtenberger C., Braga C., Sánchez-Lalinde C., Bueno C., Licarião Luna C., Rojano C., Hurtado C.M., Santos C.C., Tellaeche C., Rosa C., Bueno de Campos C., Silva C.R., Zukeran Kanda C., Jenkins N., McDonough C., Trapé Trinca C., Jaques da Cunha C., Widmer C.E., Santos C., Buscariol D., Carreira D.C., Rodrigues Carvalho D., Silva Ferraz D., Casali D., Thornton D., Rodrigues Vasconcellos D., Barcelos D., Brown D., Leal Ramos D., Oliveira Moreira D., Yogui D.R., Faria D., Sana D.A., Lidoro de Mattia D., Henz D.J., Friedeberg D., Kruger Pacheco Carvalho D.L., Astúa D., Queirolo D., Varela D., Eaton D., Matos Dias D., Rivadeneira E.F., Rocha E.C., Abreu-Júnior E.F., Carrano E., Marques Santos E., Freire Setz E.Z., Alves Ribeiro Carvalho E., Almeida Chiquito E., Matos Cardoso E., Neves Mendonça E., D’Bastiani E., Vieira E., Ramalho E.E., Guijosa-Guadarrama E., González E., Maggiorini E.V., Fischer E., Aguiar E.F., Castro E.P., Peña-Cuéllar E., Viveiros de Castro E., Brítez E.B., Vanderhoeven E.A., Pedó E., Lopes Rocha F., Girardi F., Oliveira Roque F., Dias Mazim F., Monteiro de Barros F., Martello F., Moreli Fantacini F., Pedrosa F., Bortolotto Peters F., Delborgo Abra F., Cavalcanti de Azevedo F., Silva Santos F., Guedes da Silva F., Zimmermann Teixeira F., Araujo Perini F., Passos F., Carvalho F., Cascelli de Azevedo F.C., Ferreira de Pinho F., Gonçalves, Lima F., Contreras-Moreno F., Pedroni F., Tortato F.R., Pereira Rego Santos F., Caruso F., Pereira Tirelli F., Miranda F.R., Guimarães Rodrigues F.H., Kulaif Ubaid F., Lopes Palmeira F.B., Almeida da Silva F., Grotta-Neto F., Souza F.L., Costa F.E., Pérez-Garduza F., Delsuc F., Lemos F.G., Ramirez Pinto F., Boaglio G.I., Fávero Massocato G., Preuss G., Selbach Hofmann G., Lima Aguiar G., Schuck Oliveira G., Teixeira Duarte G., Beca G., Fernandez Giné G.A., Oliveira Batista G., Gil G.E., Gonsioroski G., Secco H., Reis Medeiros H., Pfeifer Coelho I., Camboim Franceschi I., Bernardi I., Torre J.A., Zocche J.J., Seibert J.B., Faria Falcão J.C., Mangueira Dias J.H., Zorzal Nodari J., Alves Oliveira J., Ribeiro Giovanelli J.G., Pandini Favoretti J.P., Polisar J., Sponchiado J., Cherem J.J., Moreira Ramírez J.F., Toledo J.J., Barbanti Duarte J.M., Matos J.R., Arrabal J.R., Faria Oshima J.E., Fernandes Ribeiro J., Bogoni J.A., Chacón Pacheco J.A., Schuchmann K., Ferraz K., Santos Everton L., Bailey L., Oliveira Gonçalves L., Cullen L., Reis de Andrade L., Carreira Trevelin L., Bonjorne L., Almeida Rodrigues L., Leuzinger L., Neves Perillo L., Souza Araújo L., Hufnagel L., Oliveira Ribeiro L., Rezende Bernardo L.R., Rodrigues Oliveira-Santos L.G., Varzinczak L.H., Medeiros Borges L.H., Neves Guimarães L.H., Möcklinghoff L., Alvares Oliveira M., Magioli M., Assis Jardim M.M., Leite de Oliveira M., Tortato M.A., Dums M., Iezzi M.E., Ramos Pereira M.J., Jorge M.J., Castro Morini M.S., Bueno Landis M., Sampaio Xavier M., Barros M., Lima da Silva M., Rivero M., Zanin M., Marques I.M., Alves M.H., Di Bitetti M., Alvarez M., Graipel M.E., Neves Godoi M., Benedetti M.A., Guimarães Beltrão M., Coutinho Moretta Monteiro M., Paula M.J., Lages Perilli M.L., Prado da Silva M., Villar N., Moraes De Albuquerque N., Canassa N., Mota Filho N., Rosa Oliveira N., Pasqualotto N., Cáceres N.C., Attias N., Ochoa Favarini M., Santi Ribeiro O., Rodrigues Gonçalves P., Rocha P.A., Alves Condé P., Akkawi P., Koeler Lira P., Cruz P., Modenesi Ferreira P., Arroyo-Gerala P., Hartmann P.A., Tarso Zuquim Antas P., Marinho P.H., Faria Peres P.H., Peña-Mondragón J.L., Moura Lombardi P., Souza Laurindo R., Souza Cruz Alves R., Praxedes Grangeiro R.D., Lima Silva R., Beltrão-Mendes R., Twardowsky Ramalho Bonikowski R., Reppucci J., Corassa Arrais R., Sampaio R., Sartorello R., Siqueira Bovendorp R., McNab R., Espíndola Hack R.O., Assis Magalhães R., Costa Araújo R., Almeida Nobre R., León Pérez R.R., Lima Massara R., Cunha de Paula R., García Anleu R., Vieira Marques R., Dornas R., Gonçalves Rolim S., Cavalcanti S., Ramos Lima S., Ballari S., Santamaría S.B., Marques Silva S., Age S.G., Godim T., Sobral-Souza T., Bernardes Maccarini T., Ferreira Rodrigues T., Piovezan U., Cunha Tavares V., Quiroga V.A., Gasperotto Krepschi V., Picinatto Filho V., Galvão Bastazini V.A., Oliveira Gasparotto V.P., Santana Orsini V., Guedes Layme V.M., Hannibal W., Dáttilo W., Carvalho W.D., Loughry W.J., Di Blanco Y.E., Núñez-Regueiro M., Furlan Giubbina M., Passamani M., Carramaschi de Alagão Querido L., Alvez da Costa Toledo G., Kintopp Ribeiro I., Quintilham L., Bustos S., Maza J., Ferreira Lima Neto J., Von Kossel de Andrade Silva K., Sartorello L., Rampim L.E., Marás G., Camino M., Freitas-Junior M.C., Perovic P.G., Montanheiro Paolino R., Ferreira S.D., Towns V., Beraldi Esperandio I., Aximoff A., Beduschi J., Guenther M., Cassia Bianchi R., Keuroghlian-Eaton K., Lucena Mendes S., Fatima Cunha L., Cirignoli S., Ciocheti G., Alves do Prado H., Fernandes-Ferreira F., Mendes de Sena L.M., Hideki Yamane M., Brennand P., Silva R.D., Escobar S., Endo W., Reyna Hurtado R., Costa Gontijo R., Marsh L., Machado Severo M., Martinez Pardo J., Costa S.A., Leal Melo G., Gomes Santana G., Miranda Mourão G., Gaspari G.G., Duarte H., Cabral H., Silva L.H., Mendonça L., Barbosa L.L., Vieira dos Santos M., Figuerêdo Duarte Moraes M., Gordo M., Fraguas Versiani N., Cantero N., Pays O., Gonçalves Guedes P., Colas-Rosas P.F., Ribeiro P., Renaud P.C., Hoogesteijn R.J., Ayala R., Grassetto Teixeira da Cunha R., Schaub R., Laurito S., Eurich Betkowski S., Cortez S., Pereira Silva S.S., Gomes de Oliveira T., Spironello W.R., Gengler N., Mihart Hidalgo M., Juárez R., Iglesias J., Anacleto T.C., Souza Fialho M., Cavicchioli G., Barbosa Beccato M.A., Silva M., Correia Neto O., Galisteo Diemer Lopes K., Perez Godoy L., Ribeiro Luiz M., Rojas Bonzi V., Braga Ferreira G., Rabelo Oliveira M.J., Hinojosa J., Barbosa de Oliveira L.F., Nagy-Reis M.B., Fernández Ramirez S., Villas Boas Concone H., Mourthe I., Martínez-Lanfranco J., Zanoni J.B., Cruz Moreira T., Vega Guarderas Z., Bazilio S., Cervini M., Soares Pinheiro M., Gonçalves Morato R., Peroni N., Campos Trigo T., Bomfim Machado R., Gaspari F., Koenemann J., Rudolf J.C., Benchimol M., Vieira M.V., Martínez Retta L., Fernández Santiago P.G., Gonzalez Ciccia P., Cordeiro Estrela P., Carvalho S., Lustosa Esbérard C.E., Bravata-de la Cruz Y., Castro-Prieto J., Miranda Braga R., Cartes J.L., Andrade-Núñez M.J., Denkiewicz N.M., Falconi N., Brito Pezzuti J.C., Castillo Cordero H.E., Conceição de Sousa L., Gaspari Júnior R.L., Santos-Filho M., Almeida J.S., Thompson J., Silveira dos Santos J., Pereira-Ribeiro J., Burs K., Ferrari Moreira da Silva K., Velilla M., Xavier da Silva M., Sancha N., Pinheiro P.F., Volkmer de Castilho P., Bercê W., Camara Assis J., Rodrigues Tonetti V., Alves-Eigenhee M., Chinem S., Honda L., Godoy Bergallo H., Alberici V., Wallace R., Campos Krauer J.M., Ribeiro M.C., and Galetti M.
- Subjects
0106 biological sciences ,NEOTROPICAL REGION ,biodiversity hotspot ,xenarthra ,habitat loss ,Pilosa ,HABITAT LOSS ,Forest fragmentation ,XENARTHRA ,010603 evolutionary biology ,01 natural sciences ,purl.org/becyt/ford/1 [https] ,PILOSA ,Ciencias Biológicas ,Cingulata ,neotropical mammals ,purl.org/becyt/ford/1.6 [https] ,NEOTROPICAL MAMMALS ,ComputingMilieux_MISCELLANEOUS ,Ecology, Evolution, Behavior and Systematics ,forest fragmentation ,BIODIVERSITY HOTSPOT ,pilosa ,biology ,Ecology ,010604 marine biology & hydrobiology ,Xenarthra ,FOREST FRAGMENTATION ,Ecología ,biology.organism_classification ,Biodiversity hotspot ,Data set ,Geography ,Habitat destruction ,cingulata ,neotropical region ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,CINGULATA ,CIENCIAS NATURALES Y EXACTAS - Abstract
Xenarthrans -anteaters, sloths, and armadillos- have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. Have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become. Fil: Marques Santos, Paloma. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil Fil: Bocchiglieri, Adriana. Universidade Federal de Sergipe; Brasil Fil: Garcia Chiarello, Adriano. Universidade de Sao Paulo; Brasil Fil: Pereira Paglia, Adriano. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil Fil: Moreira, Adryelle. Amplo Engenharia e Gestão de Projetos ; Brasil Fil: Abba, Agustin Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Paviolo, Agustin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Gatica, Ailin. Universidad Nacional de San Luis; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Ochoa, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: de Angelo, Carlos Daniel. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias de la Tierra, Biodiversidad y Ambiente.; Argentina Fil: Tellaeche, Cintia Gisele. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Centro de Estudios Ambientales Territoriales y Sociales; Argentina. Universidad Nacional de Jujuy. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Ecorregiones Andinas; Argentina Fil: Varela, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Vanderhoeven, Ezequiel Andres. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caruso, María Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administración de Parques Nacionales. Delegación Regional del Noroeste; Argentina Fil: Arrabal, Juan Pablo. Secretaria de Gobierno de Salud. Instituto Nacional de Medicina Tropical - Sede Puerto Iguazú Misiones; Argentina. Centro de Investigaciones del Bosque Atlántico; Argentina Fil: Iezzi, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Di Bitetti, Mario Santiago. Centro de Investigaciones del Bosque Atlántico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Cruz, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina. Centro de Investigaciones del Bosque Atlántico; Argentina Fil: Reppucci, Juan Ignacio. Administración de Parques Nacionales. Delegación Regional del Noroeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Benito Santamaria, Silvia. Centro de Investigaciones del Bosque Atlántico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Quiroga, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentina Fil: Di Blanco, Yamil Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Marás, Gustavo Arnaldo. Administración de Parques Nacionales. Delegación Regional del Noroeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Camino, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Centro de Ecología Aplicada del Litoral. Universidad Nacional del Nordeste. Centro de Ecología Aplicada del Litoral; Argentina Fil: Perovic, Pablo Gastón. Administración de Parques Nacionales. Delegación Regional del Noroeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martínez Pardo, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Costa, Sebastián Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Puerto Iguazú; Argentina Fil: Pinheiro, Fabiana. Universidade Federal do Rio Grande do Sul; Brasil Fil: Volkmer de Castilho, Pedro. Universidade Federal de Santa Catarina; Brasil Fil: Bercê, William. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Camara Assis, Julia. Universidade Estadual Paulista Julio de Mesquita Filho. Faculdade de Engenharia.; Brasil Fil: Rodrigues Tonetti, Vinicius. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Alves Eigenheer, Milene. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Chinem, Simonne. Universidade de Sao Paulo; Brasil Fil: Honda, Laura K.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Bergallo, Helena de Godoy. Universidade do Estado de Rio do Janeiro; Brasil Fil: Alberici, Vinicius. Universidade de Sao Paulo; Brasil Fil: Wallace, Robert. Wildlife Conservation Society; Estados Unidos Fil: Ribeiro, Milton Cezar. Universidade de Sao Paulo; Brasil Fil: Galetti, Mauro. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
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- 2019
5. Exploring the epigenetic profile of ID4 in breast cancer: bioinformatic insights into methylation patterns and chromatin accessibility dynamics.
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Nasif D, Laurito S, Real S, and Branham MT
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- Humans, Female, Cell Line, Tumor, Tamoxifen pharmacology, Tamoxifen therapeutic use, Enhancer Elements, Genetic, Estradiol pharmacology, Inhibitor of Differentiation Proteins genetics, Inhibitor of Differentiation Proteins metabolism, Breast Neoplasms genetics, Breast Neoplasms metabolism, Breast Neoplasms pathology, Breast Neoplasms drug therapy, DNA Methylation, Epigenesis, Genetic, Computational Biology methods, Gene Expression Regulation, Neoplastic, Chromatin metabolism, Chromatin genetics, Hepatocyte Nuclear Factor 3-alpha genetics, Hepatocyte Nuclear Factor 3-alpha metabolism, Promoter Regions, Genetic
- Abstract
Purpose: Inhibitor of differentiation 4 (ID4) is a dominant-negative regulator of basic helix-loop-helix (bHLH) transcription factors. The expression of ID4 is dysregulated in various breast cancer subtypes, indicating a potential role for ID4 in subtype-specific breast cancer development. This study aims to elucidate the epigenetic regulation of ID4 within breast cancer subtypes, with a particular focus on DNA methylation and chromatin accessibility., Methods: Bioinformatic analyses were conducted to assess DNA methylation and chromatin accessibility in ID4 regulatory regions across breast cancer subtypes. Gene Set Enrichment Analysis (GSEA) was conducted to identify related gene sets. Transcription factor binding within ID4 enhancer and promoter regions was explored. In vitro experiments involved ER+ breast cancer cell lines treated with estradiol (E2) and Tamoxifen., Results: Distinct epigenetic profiles of ID4 were observed, revealing increased methylation and reduced chromatin accessibility in luminal subtypes compared to the basal subtype. Gene Set Enrichment Analysis (GSEA) implicated estrogen-related pathways, suggesting a potential link between estrogen signaling and the regulation of ID4 expression. Transcription factor analysis identified ER and FOXA1 as regulators of ID4 enhancer regions. In vitro experiments confirmed the role of ER, demonstrating reduced ID4 expression and increased methylation with estradiol treatment. Conversely, Tamoxifen treatment increased ID4 expression, indicating the potential involvement of ER signaling through ERα in the epigenetic regulation of ID4 in breast cancer cells., Conclusion: This study shows the intricate epigenetic regulation of ID4 in breast cancer, highlighting subtype-specific differences in DNA methylation and chromatin accessibility., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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6. Working together for the family: determination of HER oncogene co-amplifications in breast cancer.
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Laurito S, Branham MT, Campoy E, Real S, Cueto J, Urrutia G, Gago F, Tello O, Glatstein T, De la Iglesia P, Atanesyan L, Savola S, and Roqué M
- Abstract
HER2 is a well-studied tyrosine kinase (TK) membrane receptor which functions as a therapeutic target in invasive ductal breast carcinomas (IDC). The standard of care for the treatment of HER2-positive breast is the antibody trastuzumab. Despite specific treatment unfortunately, 20% of primary and 70% of metastatic HER2 tumors develop resistance. HER2 belongs to a gene family, with four members (HER1-4) and these members could be involved in resistance to anti-HER2 therapies. In this study we designed a probemix to detect the amplification of the four HER oncogenes in a single reaction. In addition, we developed a protocol based on the combination of MLPA with ddPCR to detect the tumor proportion of co-amplified HERs. On 111 IDC, the HER2 MLPA results were validated by FISH (Adjusted r
2 = 0,91, p < 0,0001), CISH (Adjusted r2 = 0,938, p < 0,0001) and IHC (Adjusted r2 = 0,31, p findings show that the outcome of HER2+ patients is conditioned by the status of HER3 and HER4. Our results encourage further studies to investigate the relationship with patient's response to single or combined treatment. The approach could serve as proof of principle for other tumors in which the HER oncogenes are involved.p < 0,05). Of the 111 samples, 26% presented at least one HER amplified, of which 23% showed co-amplifications with other HERs. The percentage of cells with HER2 co-amplified varied among the tumors (from 2-72,6%). Independent in-silico findings show that the outcome of HER2+ patients is conditioned by the status of HER3 and HER4. Our results encourage further studies to investigate the relationship with patient's response to single or combined treatment. The approach could serve as proof of principle for other tumors in which the HER oncogenes are involved., Competing Interests: CONFLICTS OF INTEREST The authors declare that they have no competing interests. LA and SS are employed by MRC-Holland, manufacturer of commercially available MLPA probemixes and reagents.- Published
- 2020
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7. NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics.
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Santos PM, Bocchiglieri A, Chiarello AG, Paglia AP, Moreira A, de Souza AC, Abba AM, Paviolo A, Gatica A, Medeiro AZ, Costa AN, Gallina AG, Yanosky AA, Jesus A, Bertassoni A, Rocha A, Bovo AAA, Bager A, Mol AC, Martensen AC, Faustino AC, Lopes AMC, Percequillo AR, Vogliotti A, Keuroghlian A, de la Colina MA, Devlin AL, García-Olaechea A, Sánchez A, Srbek-Araujo AC, Ochoa AC, Oliveira ACM, Lacerda ACR, Campelo AKN, de Oliveira Paschoal AM, Costa ARC, Meiga AYY, Jesus AS, Feijó A, Hirsch A, da Silva ALF, Botelho ALM, Regolin AL, Lanna AM, Nunes AV, Kindel A, Moraes AM, Gatti A, Noss AJ, Nobre AB, Montanarin A, Deffaci ÂC, de Albuquerque ACF, de Oliveira AK, Mangione AM, Pontes ARM, Bertoldi AT, Calouro AM, Desbiez ALJ, Fernandes A, Ferreguetti AC, da Silva MAA, Zimbres B, Luciano BFL, de Thoisy B, Niebuhr BBS, Papi B, Gómez-Valencia B, Santos BA, Lima BC, Oliveira BG, Santos BS, Campos BATP, Leles B, de Albuquerque França BR, Lim B, Oliveira CT, Cantagallo C, Lara CC, Lima CS, Gestich CC, de Melo-Soares CD, Peres CA, Kasper CB, Candia-Gallardo C, De Angelo C, Fragoso CE, de Freitas CH, Salvador CH, Brocardo CR, Melo CD, Leuchtenberger C, Braga C, Sánchez-Lalinde C, Bueno C, Luna CL, Rojano C, Hurtado CM, Dos Santos CC, Tellaeche C, Rosa C, de Campos CB, Silva CR, Kanda CZ, Jenkins CN, McDonough C, Trinca CT, da Cunha CJ, Widmer CE, Santos C, Buscariol D, Carreira DC, Carvalho DR, da Silva Ferraz D, Casali D, Thornton D, Vasconcellos DR, Barcelos D, Brown D, Ramos DL, Moreira DO, Yogui DR, Faria D, Sana DA, de Mattia DL, Henz DJ, Friedeberg DB, Carvalho DLKP, Astúa D, Queirolo D, Varela DM, Eaton DP, Dias DM, Rivadeneira EF, Rocha EC, de Abreu-Júnior EF, Carrano E, Santos EM Jr, Setz EZF, Carvalho EAR Jr, de Almeida Chiquito E, de Matos Cardoso E, Mendonça EN, D'Bastiani E, Vieira EM, Ramalho EE, Guijosa-Guadarrama E, González E, Maggiorini EV, Fischer E, Aguiar EF, Castro ÉP, de la Peña-Cuéllar E, de Castro EBV, Brítez EB, Vanderhoeven EA, Pedó E, Rocha FL, Girardi F, de Oliveira Roque F, Mazim FD, de Barros FM, Martello F, Fantacini FM, Pedrosa F, Peters FB, Abra FD, de Azevedo FC, da Silva Santos F, da Silva FG, Teixeira FZ, Perini FA, Passos FC, Carvalho F, de Azevedo FCC, de Pinho FF, Gonçalves F, Lima F, Contreras-Moreno FM, Pedroni F, Tortato FR, Santos FPR, Caruso F, Tirelli FP, Miranda FR, Rodrigues FHG, Ubaid FK, Palmeira FBL, da Silva FA, Grotta-Neto F, de Souza FL, Costa FE, Pérez-Garduza F, Delsuc F, Lemos F, Pinto FR, Boaglio GI, Massocato GF, Preuss G, Hofmann GS, Aguiar GL, Oliveira GS, Duarte GT, Beca G, Giné GAF, Batista GO, Gil GE, Gonsioroski G, Secco H, Medeiros HR, Coelho IP, Franceschi IC, Bernardi I, de la Torre JA, Zocche JJ, Seibert JB, de Faria Falcão JC, Dias JHM, Nodari JZ, Oliveira JA, Giovanelli JGR, Favoretti JPP, Polisar J, Sponchiado J, Cherem JJ, Ramírez JFM, de Toledo JJ, Duarte JMB, de Matos JR, Arrabal JP, de Faria Oshima JE, Ribeiro JF, Bogoni JA, Pacheco JJC, Schuchmann KL, Ferraz KMPMB, Dos Santos Everton L, Bailey LL, Gonçalves LO, Cullen L Jr, de Andrade LR, Trevelin LC, Bonjorne L, de Almeida Rodrigues L, Leuzinger L, Perillo LN, Araújo LS, Hufnagel L, Ribeiro LO, Bernardo LRR, Oliveira-Santos LGR, Varzinczak LH, Borges LHM, Guimarães LN, Möcklinghoff L, Oliveira MA, Magioli M, de Assis Jardim MM, de Oliveira ML, Tortato MA, Dums M, Iezzi ME, Pereira MJR, Jorge ML, de Castro Morini MS, Landis MB, Xavier MS, Barros MAS, da Silva ML, Rivero M, Zanin M, Marques MI, Alves MH, Di Bitetti MS, Alvarez MR, Graipel ME, Godoi MN, Benedetti MA, Beltrão MG, Monteiro MCM, de Paula MJ, Perilli MLL, da Silva MP, Villar N, De Albuquerque NM, Canassa NF, Filho NM, da Rosa Oliveira N, Pasqualotto N, Cáceres NC, Attias N, Favarini MO, Ribeiro OS, Gonçalves PR, da Rocha PA, Condé PA, Akkawi P, Cruz P, Lira PK, Ferreira PM, Arroyo-Gerala P, Hartmann PA, de Tarso Zuquim Antas P, Marinho PH, de Faria Peres PH, Peña-Mondragón JL, Lombardi PM, de Souza Laurindo R, Alves RSC, Grangeiro RDP, Silva RL, Beltrão-Mendes R, Bonikowski RTR, Reppucci J, Arrais RC, Sampaio R, Sartorello R, Bovendorp RS, McNab R, Hack ROE, Magalhães RA, Araújo RC, de Almeida Nobre R, Pérez RRL, Massara RL, de Paula RC, Anleu RG, Marques RV, Dornas R, Rolim SG, Cavalcanti SMC, Lima SR, Ballari SA, Santamaría SB, Silva SM, Age SG, Godim T, Sobral-Souza T, Maccarini TB, Rodrigues TF, Piovezan U, Tavares VDC, Quiroga VA, Krepschi VG, Filho VP, Bastazini VAG, de Oliveira Gasparotto VP, Orsini VS, Layme VMG, Hannibal W, Dáttilo W, de Carvalho WD, Loughry WJ, Di Blanco YE, Núñez-Regueiro MM, Giubbina MF, Passamani M, de Alagão Querido LC, da Costa Toledo GA, Ribeiro IK, Quintilham L, de Bustos S, de la Maza J, Neto JFL, de Andrade Silva KVK, Sartorello L, Rampim LE, Marás GA, Camino M, Freitas-Junior M, Perovic PG, Paolino RM, Ferreira SD, Towns V, Esperandio IB, Aximoff I, Beduschi J, Guenther M, de Cassia Bianchi R, Keuroghlian-Eaton S, Mendes SL, de Fatima Cunha L, Cirignoli S, Ciocheti G, do Prado HA, Fernandes-Ferreira H, de Sena LMM, Yamane MH, Brennand PGG, da Silva RD, Escobar S, Endo W, Hurtado RR, Gontijo NRC, Marsh LK, Severo MM, Pardo JM, Costa SA, Melo GL, Santana GG, de Miranda Mourão G, Gaspari GG, Duarte H, Cabral H, da Silva LH, Mendonça L, Barbosa LL, Dos Santos MV, Moraes MFD, Gordo M, Versiani NF, Cantero N, Pays O, Guedes PG, Colas-Rosas PF, Ribeiro P, Renaud PC, Hoogesteijn RJ, Ayala R, da Cunha RGT, Schaub R, Laurito S, Betkowski SE, Cortez S, Silva SSP, de Oliveira TG, Spironello WR, Gengler N, Hidalgo MM, Juárez R, Iglesias JA, Anacleto TC, de Souza Fialho M, Cavicchioli G, Beccato MAB, Silva MD, Neto OC, Lopes KGD, Godoy LP, Luiz MR, Rojas Bonzi VB, Ferreira GB, Oliveira MJR, Hinojosa J, de Oliveira LFB, Nagy-Reis MB, Ramirez SF, Concone HVB, Mourthe I, Martínez-Lanfranco JA, Zanoni JB, Moreira TC, Guarderas ZV, Bazilio S, Cervini M, Pinheiro MS, Morato RG, Peroni N, Trigo TC, Machado RB, Gaspari F, Koenemann JG, Rudolf JC, Benchimol M, Vieira MV, Retta LM, Santiago PGF, Ciccia PG, Estrela PC, Carvalho S, Esbérard CEL, de la Cruz YB, Castro-Prieto J, Braga RM, Cartes JL, Andrade-Núñez MJ, Denkiewicz NM, Falconi N, Pezzuti JCB, Del Castillo Cordero HF, de Sousa LC, de Gaspari Júnior RL, Santos-Filho M, Almeida JS, Thompson JJ, Dos Santos JS, Pereira-Ribeiro J, Burs K, da Silva KFM, Velilla M, da Silva MX, de la Sancha NU, Pinheiro PF, de Castilho PV, Bercê W, Assis JC, Tonetti VR, Alves-Eigenheer M, Chinem S, Honda LK, de Godoy Bergallo H, Alberici V, Wallace R, Krauer JMC, Ribeiro MC, and Galetti M
- Abstract
Xenarthrans-anteaters, sloths, and armadillos-have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data., (© 2019 The Authors. Ecology © 2019 The Ecological Society of America.)
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- 2019
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8. PAX6 Promoter Methylation Correlates with MDA-MB-231 Cell Migration, and Expression of MMP2 and MMP9
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Urrutia G, Laurito S, Campoy E, Nasif D, Branham MT, and Roqué M
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- Cell Line, Tumor, Cell Movement genetics, Female, Gene Expression Regulation, Neoplastic genetics, Humans, MCF-7 Cells, Breast Neoplasms genetics, DNA Methylation genetics, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 9 genetics, PAX6 Transcription Factor genetics, Promoter Regions, Genetic genetics
- Abstract
Objective: Breast cancer is a heterogeneous disease characterized by an accumulation of genetic and epigenetic alterations that lead tumor cells to acquire characteristics like the capacity for invasion and metastasis. Metastasis remains a major challenge in cancer management and understanding of its molecular basis should result in improved prevention, diagnosis, and treatment of breast cancer patients. The aim of this study was to investigate how promoter DNA methylation regulates PAX6 gene expression and influences breast carcinoma cell migration. Methods: PAX6 promoter methylation was detected by Methyl Specific-Multiplex Ligation Probe Amplification (MS-MLPA). Gene expression was evaluated using qRT-PCR, while the effect of PAX6 on migration was ssessed by wound healing assay. In addition, MMP2 and MMP9 genes were studied using different bioinformatic tools. Results: The PAX6 promoter is methylated in breast cancer cell lines and methylation in this region impacts on its expression. Migration assays revealed that PAX6 overexpression promotes cell migration, while PAX6 inhibition decreases it. More importantly, we found that migration is affected by PAX6 methylation status. Employing bioinformatic analysis, binding sites for PAX6 on the regulatory regions of the MMP2 and MMP9 genes were established, PAX6 overexpression increasing MMP2 and MMP9 expression at the mRNA level. Conclusion: Our study provides novel insights into epigenetic events that regulate PAX6 expression and molecular mechanisms by which PAX6 modifies the migration capacity of breast cancer cells., (Creative Commons Attribution License)
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- 2018
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9. Epigenetic regulation of ID4 in breast cancer: tumor suppressor or oncogene?
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Nasif D, Campoy E, Laurito S, Branham R, Urrutia G, Roqué M, and Branham MT
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- Breast Neoplasms metabolism, Cell Line, Tumor, Cell Movement, Epigenesis, Genetic, Female, Gene Expression Regulation, Neoplastic, Humans, MCF-7 Cells, Prognosis, Receptors, Estrogen metabolism, Signal Transduction, Survival Analysis, Breast Neoplasms genetics, DNA Methylation, Down-Regulation, Inhibitor of Differentiation Proteins genetics
- Abstract
Background: Inhibitor of differentiation protein 4 (ID4) is a dominant negative regulator of the basic helix-loop-helix (bHLH) family of transcription factors. During tumorigenesis, ID4 may act as a tumor suppressor or as an oncogene in different tumor types. However, the role of ID4 in breast cancer is not clear where both an oncogenic and a tumor suppressor function have been attributed. Here, we hypothesize that ID4 behaves as both, but its role in breast differs according to the estrogen receptor (ER) status of the tumor., Methods: ID4 expression was retrieved from TCGA database using UCSC Xena. Association between overall survival (OS) and ID4 was assessed using Kaplan-Meier plotter. Correlation between methylation and expression was analyzed using the MEXPRESS tool. In vitro experiments involved ectopic expression of ID4 in MCF-7, T47D, and MDA-MB231 breast cancer cell lines. Migration and colony formation capacity were assessed after transfection treatments. Gene expression was analyzed by ddPCR and methylation by MSP, MS-MLPA, or ddMSP., Results: Data mining analysis revealed that ID4 expression is significantly lower in ER+ tumors with respect to ER- tumors or normal tissue. We also demonstrate that ID4 is significantly methylated in ER+ tumors. Kaplan-Meier analysis indicated that low ID4 expression levels were associated with poor overall survival in patients with ER+ tumors. In silico expression analysis indicated that ID4 was associated with the expression of key genes of the ER pathway only in ER+ tumors. In vitro experiments revealed that ID4 overexpression in ER+ cell lines resulted in decreased migration capacity and reduced number of colonies. ID4 overexpression induced a reduction in ER levels in ER+ cell lines, while estrogen deprivation with fulvestrant did not induce changes neither in ID4 methylation nor in ID4 expression., Conclusions: We propose that ID4 is frequently silenced by promoter methylation in ER+ breast cancers and functions as a tumor suppressor gene in these tumors, probably due to its interaction with key genes of the ER pathway. Our present study contributes to the knowledge of the role of ID4 in breast cancer.
- Published
- 2018
- Full Text
- View/download PDF
10. [Variation analysis of the number of copies and methylene patterns in region 15q11-q13].
- Author
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Laurito S and Roqué M
- Subjects
- Gene Deletion, Gene Duplication, Humans, Angelman Syndrome genetics, Chromosomes, Human, Pair 15 genetics, DNA Copy Number Variations genetics, Prader-Willi Syndrome genetics, Uniparental Disomy genetics
- Abstract
Human chromosome 15q11-q13 region is prone to suffer genetic alterations. Some genes of this region have a differential monoallelic imprinting-regulated expression pattern. Defects in imprinting regulation (IE), uniparental disomy (UPD) or copy number variation (CNV) due to chromosomal breakpoints (BP) in 15q11-q13 region, are associated with several diseases. The most frequent are Prader-Willi syndrome, Angelman syndrome and 15q11-q13 microduplication syndrome. In this work, we analyzed DNA samples from 181 patients with phenotypes which were compatible with the above-mentioned diseases, using Methyl specific-multiplex ligation-dependent probe amplification (MS-MLPA). We show that, of the 181 samples, 39 presented alterations detectable by MS-MLPA. Of those alterations, 61.5% (24/39) were deletions, 5.1% (2/39) duplications and 33.3% (13/39) UPD/IE. The CNV cases were 4 times more frequent than UPD/IE (OR= 4; IC 95%: 1.56-10.25), consistent with the literature. Among the CNVs, two atypical cases allow to postulate new possible BP sites that have not been reported previously in the literature.
- Published
- 2018
11. Epigenetic variations in breast cancer progression to lymph node metastasis.
- Author
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Urrutia G, Laurito S, Marzese DM, Gago F, Orozco J, Tello O, Branham T, Campoy EM, and Roqué M
- Subjects
- Biomarkers, Tumor, Breast pathology, Cohort Studies, CpG Islands, DNA Methylation, Disease Progression, Eye Proteins metabolism, Female, Homeodomain Proteins metabolism, Humans, Lymph Nodes pathology, PAX6 Transcription Factor, Paired Box Transcription Factors metabolism, Prognosis, Repressor Proteins metabolism, Breast Neoplasms genetics, Breast Neoplasms metabolism, Epigenesis, Genetic, Gene Expression Regulation, Neoplastic, Lymphatic Metastasis
- Abstract
Breast cancer is a heterogeneous disease characterized by the accumulation of genetic and epigenetic alterations that contribute to the development of regional and distant metastases. Lymph node metastasis (LNM) status is the single most important prognostic factor. Metastatic cancer cells share common molecular alterations with those of the primary tumor, but in addition, they develop distinct changes that allow the cancer to progress. There is an urgent need for molecular studies which focus on identifying genomic and epigenomic markers that can predict the progression to metastasis. The objective of this study was to identify epigenetic similarities and differences between paired primary breast tumor (PBT) and LNM. We employed Methylation-Specific-MLPA (Multiplex ligation-dependent probe amplification) to assess the methylation status of 33 cancer-related genes in a cohort of 50 paired PBT and LNM specimens. We found that the methylation index, which represents the degree of aberrantly methylated genes in a specimen, was maintained during the progression to LNM. However, some genes presented differential methylation profiles. Interestingly, PAX6 presented a significant negative correlation between paired PBT and LNM (p = 0.03), which indicated a switch from methylated to unmethylated status in the progression from PBT to LNM. We further identified that the methylation status of PAX6 on the identified CpG site functionally affected the expression of PAX6 at the mRNA level. Our study unraveled significant epigenetic changes during the progression from PBT to LNM, which may contribute to improved prognosis, prediction and therapeutic management of metastatic breast cancer patients.
- Published
- 2015
- Full Text
- View/download PDF
12. [Neurofibromatosis type 1. Splicing mutation detected by MLPA and DNA sequencing in Argentina].
- Author
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Laurito S, Di Pierri J, and Roqué M
- Subjects
- Adolescent, Algorithms, Argentina, Female, Humans, Indians, South American, White People, Multiplex Polymerase Chain Reaction, Mutation, Missense, Neurofibromatosis 1 genetics, RNA Splicing, Sequence Analysis, DNA
- Abstract
Neurofibromatosis type 1 (NF1) is a dominant autosomic genetic disorder, with a birth incidence of 1 in 2500-3000. Diagnosis is difficult because of the size of gene NF1 that has few hot-spots sites, the absence of a clear genotype-phenotype relation, and a heterogeneous clinical manifestation. A NF1 suspected case from Jujuy province was analyzed by multiplex ligation-dependent probe amplification (MLPA). Mestizo female teenage (Amerindian/European), with a maxilar osteoma, lumbar lordosis, cutaneous neurofibromas and café au lait spots. MLPA detected an alteration in exon 13 of the NF1 gene. By sequencing of exon 13, a missense mutation (NM_000267.3:c.1466A>G) was found which introduces an aberrant splicing site and is registered as pathogenic in the clinical variants database of NCBI. As far as we are aware, this is the first report of a NF1 mutation in mestizo population of Northwest Argentina. 1466A>G has been described before in patients of European origin, suggesting that the affected site could be a hot-spot site of the gene. For countries as Argentina, with limited availability of molecular diagnostic methods, we propose a diagnosis algorithm by starting the mutational analysis of NF1 with MLPA. This methodology is relatively simple and of low cost, avoiding to send samples abroad for genetic analyses.
- Published
- 2015
13. [Detection of a Williams Beuren syndrome case by MLPA].
- Author
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Laurito S, Branham T, Herrero G, Marsa S, Garro F, and Roqué M
- Subjects
- Aortic Stenosis, Supravalvular diagnosis, Child, Preschool, Gene Dosage, Humans, In Situ Hybridization, Fluorescence, Male, Williams Syndrome genetics, Multiplex Polymerase Chain Reaction, Williams Syndrome diagnosis
- Abstract
Williams-Beuren syndrome (WBS) is a rare developmental disorder characterized by distinctive facial, neurobehavioral, and cardiovascular features. WBS is caused by a heterozygous contiguous gene microdeletion of the WBS crítical region on chromosome 7q11.23. Confirmation of clinical suspicion is essential for clinical monitoring of the patient and genetic counseling of the family. Fluorescence in situ hybridization (FISH) is considered the gold standard technique for detecting WBS. Multiplex ligation-dependent probe amplification (MLPA) has been introduced into DNA diagnostic laboratories for the detection of copy number variations in several diseases including WBS. The objective of this study was to confirm, by MLPA, the clinical diagnosis of WBS in a pediatric patient. This technique allowed to detect the deletion of CYLN2, FZD9, STX1A, ELN, LIMK1 and RFC2 genes. In geographic regions were the detection by FISH is not available for this disease, the MLPA methodology allowed to confirm the clinic diagnostic of WBS. To our knowledge this is the first report demonstrating the confirmation of WBS by MLPA in Argentina.
- Published
- 2013
14. [Deletion of the LIS1, ASPA, TRPV1 and CAMTA2 genes in region 17p13.3 in a patient with Miller-Dieker syndrome].
- Author
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Laurito S, Goldschmidt E, Marquez M, and Roque M
- Subjects
- Calcium-Binding Proteins genetics, DNA Mutational Analysis, Humans, Infant, Male, Phenotype, Trans-Activators genetics, 1-Alkyl-2-acetylglycerophosphocholine Esterase genetics, Chromosomes, Human, Pair 17 genetics, Classical Lissencephalies and Subcortical Band Heterotopias genetics, Microtubule-Associated Proteins genetics, TRPV Cation Channels genetics
- Published
- 2011
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