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2. P595 Time of Therapeutic Drug Monitoring of infliximab during induction and its relationship with clinical remission in Inflammatory Bowel Disease

Author

C Muñoz-Villafranca, P Arreba, M Gomez, N Ispizua Madariaga, L Buendia, M L Lopez, A Ugarte, I de la Fuente, N Prado, L Irusta, M A Jimenez, M Santa Cruz, M Gonzalez, A Bilbao, and J Ortiz de Zárate

Subjects
Gastroenterology, General Medicine
Abstract

Background The usefulness of therapeutic drug monitoring(TDM) of infliximab during induction and its relationship with clinical outcome in patients with inflammatory bowel disease(IBD) is well established. However, the optimal time of monitoring in terms of clinical outcomes remains unclear. The aim is to evaluate the TDM at various times during induction and its association with clinical remission. Methods An observational, retrospective and single-centre study of patients with active disease ,Crohn’s disease (CD) or ulcerative colitis(UC), was performed. Induction treatment with infliximab 0, 2, 6 weeks was conducted and if there was response, maintenance therapy was provided. Antibody levels were measured in serum samples at week 2 and week 14 by using an enzyme-linked immunosorbent assay, within the therapeutic range(3-8 µg/ml). Patients were followed at least for one year or until loss of response. The clinical response was assessed in week 14 and week 52 by Harvey-Bradshaw Index in CD and Mayo Partial Index in UC. Results Out of 93 patients that were evaluated, 23 with UC(24.73%) and 70 with CD(75.27%,), 65 (69.89%) and 58 patients(62.37%) achieved remission at week 14 and week 52 respectively. Infliximab levels at week 14 but not at week 2 were significantly higher in patients who achieved clinical remission in both week 14 (5.8 vs 2.1 µg/ml; p=0.0002) as in week 52 (6.4 vs 1.9 µg/ml; p=0.0001). The levels at week 14, but not at week 2 had predictive value of remission, in both week 14 [OR 1,4 (CI 1.14-1.76) p=0.001] and week 52 [OR 1.4 (CI 1.15-1.87); p=0.001]. The patients with immunosuppressants at week 14 presented the highest rate of remission in week 52 (77.78 vs 52.63; p=0.014). The intensification of treatment was a negative factor to achieve remission both in week 14 and at week 52. Conclusion The levels of infliximab in week 14 are higher and have predictive value of clinical remission both in early (week 14) and long-term (week 52). Levels of infliximab in week 2 did not provide significant predictive value of clinical response. The use of immunosuppressants could be a favourable factor of clinical outcome.

Published
2023
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3. Trans-metacarpophalangeal traction to avoid finger-related complications during wrist arthroscopy: Feasibility study with 20 cadaver wrists

Author

S. Hendriks, J.J. Hidalgo Diaz, Sybille Facca, L. Buendia, P.A. Liverneaux, Ghada Asmar, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Wrist Joint, medicine.medical_specialty, genetic structures, Joint spaces, medicine.medical_treatment, Wrist, Metacarpophalangeal Joint, Arthroscopy, 03 medical and health sciences, Postoperative Complications, 0302 clinical medicine, Traction, Cadaver, medicine, Wrist arthroscopy, Humans, Orthopedics and Sports Medicine, 030212 general & internal medicine, Orthodontics, 030222 orthopedics, medicine.diagnostic_test, business.industry, Rehabilitation, [PHYS.MECA]Physics [physics]/Mechanics [physics], Traction (orthopedics), Phalanx, eye diseases, Surgery, body regions, surgical procedures, operative, medicine.anatomical_structure, Midcarpal joint, Feasibility Studies, sense organs, business
Abstract

Some of the iatrogenic complications of wrist arthroscopy are related to the traction applied to the fingers. The aim of this work was to test whether trans-metacarpophalangeal (trans-MCP) traction would provide sufficient distraction of the radiocarpal and midcarpal joint spaces to perform wrist arthroscopy without exerting any mechanical stress on the fingers, from the metacarpophalangeal (MCP) joints to the distal phalanges. Our study included 20 cadaveric wrists mounted on an arthroscopic traction tower maintained by finger traps placed on the 2nd and 4th fingers. For each wrist, three fluoroscopic views were taken: without traction, with finger traction (78.45 N), with trans-MCP traction (78.45 N). The average height of the midcarpal joint space was 0.8305 cm without traction, 1.037 cm with finger traction, and 1.1 cm with trans-MCP traction. The height of the radiocarpal joint space averaged 0.853 cm without traction, 1.167 cm with finger traction, and 1.187 cm with trans-MCP traction. There were no differences between the heights of joint spaces between digital and trans-MCP traction. Our results show that trans-MCP traction provides distraction of the wrist joint spaces equivalent to that obtained with finger traction. However, we still need to develop a trans-MCP traction device usable in clinical practice.

Published
2017
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8. Implication of Climate Change in the Philippine Agriculture

Author

C. Escaño, L. Buendia, and A. Valdeavilla

Subjects
Atmospheric Science, business.industry, Yield (finance), Climatic variables, Climate change, Agriculture, Climatology, General Circulation Model, Evapotranspiration, Environmental science, Precipitation, Baseline (configuration management), business, Agronomy and Crop Science
Abstract

The study was conducted in three major rice producing areas to assess the probable impact of climate change in Philippine rice production. Climate change scenarios were devised using general circulation models (GCMs) from ratios of climate variables (temperature, precipitation, and solar radiation) in the doubled CO2 runs to the current climate variables applied to observed weather data for each site.The GCMs used were the Goddard Institute for Space Studies (GISS), the Geophysical Fluid Dynamics Laboratory (GFDL), and the United Kingdom Meteorological Office (UKMO). The CERES-Rice IRSNAT Crop Model was modified to simulate the changes in photosynthesis and evapotranspiration caused by increases in atmospheric CO2. Sensitivity study was performed using step increases in temperature of + 2°C and +4°C combined with step changes in precipitation of +20% and -20%.Using the mean yield from three sites, results show that under scenario of climate change alone (330ppm of CO2), an average reduction in rice baseline yield of 22% will be experienced in the Philippines. However, under climate change with physiological CO2 effects (555ppm of CO2), an increase in baseline yield (at 330ppm level of CO2) of only 1% will a obtained. Increasing only the temperature by 2°C without any change in precipitation at 330ppm CO2 level will correspond to 21% reduction in yield. Further increase in temperature of 4°C will reduce the baseline yield by 39%. Changes in precipitation of -20% and +20% did not significantly affect the rice yields. Considering, however, the physiological effects of CO2 for sensitivity analysis, rice yields increased by 35% (+0°C), 42% (+2°C), and 58% (+4°C).Overall, results indicate a wide variation of regional responses to climate change. The Southern part seemed to be more vulnerable to climate change than the Northern Philippines.

Published
1993
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9. [The kidney in mechanical ventilation]

Author

J A, Gómez Rubí, S, Nicolás Franco, J L, Buendia, and J C, Pardo Talavera

Subjects
Pulmonary Circulation, Acute Disease, Animals, Humans, Renal Insufficiency, Kidney, Respiration, Artificial
Abstract

The negative effect of artificial ventilation with positive pressure on renal function, expresses itself as a decrease of water and sodium excretion, being directly related with the raise of intrathoracic pressure. Factors participating in this process are: lowering in cardiac output, arousal of sympathic nervous system, increase in vasopressin action, activation of renin-angiotensin-aldosterone system and decrease of atrial natriuretic peptide release. This disorder of hydromineral metabolism produces: Impairment of hemodynamic equilibrium, favors the increase of hypoxia and renal failure. The effects of mechanical ventilation on renal function can be attenuated with the adoption of the following measures: a) techniques (use of low levels of PEEP and early disconnection of respirator); b) therapeutic (dopamine 2-3 mcg/kg/min, rational use of diuretics and fluids); y c) monitoring of renal function and hydro-mineral equilibrium.

Published
1992

10. ESICM LIVES 2016: part one: Milan, Italy. 1-5 October 2016

Author

Bos, L., Schouten, L., van Vught, L., Wiewel, M., Ong, D., Cremer, O., Artigas, A., Martin-Loeches, I., Hoogendijk, A., van der Poll, T., Horn, J., Juffermans, N., Schultz, M., de Prost, N., Pham, T., Carteaux, G., Dessap, A. Mekontso, Brun-Buisson, C., Fan, E., Bellani, G., Laffey, J., Mercat, A., Brochard, L., Maitre, B., Howells, P. A., Thickett, D. R., Knox, C., Park, D. P., Gao, F., Tucker, O., Whitehouse, T., McAuley, D. F., Perkins, G. D., Pisani, L., Roozeman, J. P., Simonis, F. D., Giangregorio, A., Schouten, L. R., Van der Hoeven, S. M., Neto, A. Serpa, Festic, E., Dondorp, A. M., Grasso, S., Bos, L. D., Schultz, M. J., Koster-Brouwer, M., Verboom, D., Scicluna, B., van de Groep, K., Frencken, J., Bonten, M., Ko, J. I., Kim, K. S., Suh, G. J., Kwon, W. Y., Kim, K., Shin, J. H., Ranzani, O. T., Prina, E., Menendez, R., Ceccato, A., Mendez, R., Cilloniz, C., Gabarrus, A., Ferrer, M., Torres, A., Urbano, A., Zhang, L. A., Swigon, D., Pike, F., Parker, R. S., Clermont, G., Scheer, C., Kuhn, S. O., Modler, A., Vollmer, M., Fuchs, C., Hahnenkamp, K., Rehberg, S., Gründling, M., Taggu, A., Darang, N., Öveges, N., László, I., Tánczos, K., Németh, M., Lebák, G., Tudor, B., Érces, D., Kaszaki, J., Huber, W., Trásy, D., Molnár, Z., Ferrara, G., Edul, V. S. Kanoore, Canales, H. S., Martins, E., Canullán, C., Murias, G., Pozo, M. O., Eguillor, J. F. Caminos, Buscetti, M. G., Ince, C., Dubin, A., Aya, H. D., Rhodes, A., Fletcher, N., Grounds, R. M., Cecconi, M., Jacquet-Lagrèze, M., Riche, M., Schweizer, R., Portran, P., Fornier, W., Lilot, M., Neidecker, J., Fellahi, J. L., Escoresca-Ortega, A., Gutiérrez-Pizarraya, A., Charris-Castro, L., Corcia-Palomo, Y., Fernandez-Delgado, E., Garnacho-Montero, J., Roger, C., Muller, L., Elotmani, L., Lipman, J., Lefrant, J. Y., Roberts, J. A., Muñoz-Bermúdez, R., Samper, M., Climent, C., Vasco, F., Sara, V., Luque, S., Campillo, N., Cerrato, S. Grau, Masclans, J. R., Alvarez-Lerma, F., Brugger, S. Carvalho, Jimenez, G. Jimenez, Torner, M. Miralbés, Cabello, J. Trujillano, Garrido, B. Balsera, Casals, X. Nuvials, Gaite, F. Barcenilla, Vidal, M. Vallverdú, Martínez, M. Palomar, Gusarov, V., Shilkin, D., Dementienko, M., Nesterova, E., Lashenkova, N., Kuzovlev, A., Zamyatin, M., Demoule, A., Carreira, S., Lavault, S., Palancca, O., Morawiec, E., Mayaux, J., Arnulf, I., Similowski, T., Rasmussen, B. S., Maltesen, R. G., Hanifa, M., Pedersen, S., Kristensen, S. R., Wimmer, R., Panigada, M., Bassi, G. Li, Kolobow, T., Zanella, A., Cressoni, M., Berra, L., Parrini, V., Kandil, H., Salati, G., Livigni, S., Amatu, A., Andreotti, A., Tagliaferri, F., Moise, G., Mercurio, G., Costa, A., Vezzani, A., Lindau, S., Babel, J., Cavana, M., Consonni, D., Pesenti, A., Gattinoni, L., Mansouri, P., Zand, F., Zahed, L., Dehghanrad, F., Bahrani, M., Ghorbani, M., Cambiaghi, B., Moerer, O., Mauri, T., Kunze-Szikszay, N., Ritter, C., Quintel, M., Vilander, L. M., Kaunisto, M. A., Vaara, S. T., Pettilä, V., Mulier, J. L. G. Haitsma, Rozemeijer, S., Spoelstra-de Man, A. M. E., Elbers, P. E., Tuinman, P. R., de Waard, M. C., Oudemans-van Straaten, H. M., Liberatore, A. M. A., Souza, R. B., Martins, A. M. C. R. P. F., Vieira, J. C. F., Koh, I. H. J., Martínez, M. Galindo, Sánchez, R. Jiménez, Gascón, L. Martínez, Mulero, M. D. Rodríguez, Freire, A. Ortín, Muñoz, A. Ojados, Acebes, S. Rebollo, Martínez, Á. Fernández, Aliaga, S. Moreno, Para, L. Herrera, Payá, J. Murcia, Mulero, F. Rodríguez, Guerci, P., Ince, Y., Heeman, P., Ergin, B., Uz, Z., Massey, M., Papatella, R., Bulent, E., Toraman, F., Longbottom, E. R., Torrance, H. D., Owen, H. C., Hinds, C. J., Pearse, R. M., O’Dywer, M. J., Trogrlic, Z., van der Jagt, M., Lingsma, H., Ponssen, H. H., Schoonderbeek, J. F., Schreiner, F., Verbrugge, S. J., Duran, S., van Achterberg, T., Bakker, J., Gommers, D. A. M. P. J., Ista, E., Krajčová, A., Waldauf, P., Duška, F., Shah, A., Roy, N., McKechnie, S., Doree, C., Fisher, S., Stanworth, S. J., Jensen, J. F., Overgaard, D., Bestle, M. H., Christensen, D. F., Egerod, I., Pivkina, A., Zhivotneva, I., Pasko, N., Alklit, A., Hansen, R. L., Knudsen, H., Grode, L. B., Hravnak, M., Chen, L., Dubrawski, A., Pinsky, M. R., Parry, S. M., Knight, L. D., Connolly, B. C., Baldwin, C. E., Puthucheary, Z. A., Denehy, L., Hart, N., Morris, P. E., Mortimore, J., Granger, C. L., Jensen, H. I., Piers, R., Van den Bulcke, B., Malmgren, J., Metaxa, V., Reyners, A. K., Darmon, M., Rusinova, K., Talmor, D., Meert, A. P., Cancelliere, L., Zubek, L., Maia, P., Michalsen, A., Decruyenaere, J., Kompanje, E., Vanheule, S., Azoulay, E., Vansteelandt, S., Benoit, D., Ryan, C., Dawson, D., Ball, J., Noone, K., Aisling, B., Prudden, S., Ntantana, A., Matamis, D., Savvidou, S., Giannakou, M., Gouva, M., Nakos, G., Koulouras, V., Aron, J., Lumley, G., Milliken, D., Dhadwal, K., McGrath, B. A., Lynch, S. J., Bovento, B., Sharpe, G., Grainger, E., Pieri-Davies, S., Wallace, S., McGrath, B., Jung, M., Cho, J., Park, H., Suh, G., Kousha, O., Paddle, J., Gripenberg, L. Gamrin, Rehal, M. Sundström, Wernerman, J., Rooyackers, O., de Grooth, H. J., Choo, W. P., Spoelstra-de Man, A. M., Swart, E. L., Talan, L., Güven, G., Altıntas, N. D., Padar, M., Uusvel, G., Starkopf, L., Starkopf, J., Blaser, A. Reintam, Kalaiselvan, M. S., Arunkumar, A. S., Renuka, M. K., Shivkumar, R. L., Volbeda, M., ten Kate, D., Hoekstra, M., van der Maaten, J. M., Nijsten, M. W., Komaromi, A., Norberg, Å., Smedberg, M., Mori, M., Pettersson, L., Theodorakopoulou, M., Christodoulopoulou, T., Diamantakis, A., Frantzeskaki, F., Kontogiorgi, M., Chrysanthopoulou, E., Lygnos, M., Diakaki, C., Armaganidis, A., Gundogan, K., Dogan, E., Coskun, R., Muhtaroglu, S., Sungur, M., Ziegler, T., Guven, M., Kleyman, A., Khaliq, W., Andreas, D., Singer, M., Meierhans, R., Schuepbach, R., De Brito-Ashurst, I., Sabetian, G., Nikandish, R., Hagar, F., Masjedi, M., Maghsudi, B., Vazin, A., Asadpour, E., Kao, K. C., Chiu, L. C., Hung, C. Y., Chang, C. H., Li, S. H., Hu, H. C., El Maraghi, S., Ali, M., Rageb, D., Helmy, M., Marin-Corral, J., Vilà, C., Vàzquez, A., Martín-Loeches, I., Díaz, E., Yébenes, J. C., Rodriguez, A., Álvarez-Lerma, F., Varga, N., Cortina-Gutiérrez, A., Dono, L., Martínez-Martínez, M., Maldonado, C., Papiol, E., Pérez-Carrasco, M., Ferrer, R., Nweze, K., Morton, B., Welters, I., Houard, M., Voisin, B., Ledoux, G., Six, S., Jaillette, E., Nseir, S., Romdhani, S., Bouneb, R., Loghmari, D., Aicha, N. Ben, Ayachi, J., Meddeb, K., Chouchène, I., Khedher, A., Boussarsar, M., Chan, K. S., Yu, W. L., Nolla, J., Vidaur, L., Bonastre, J., Suberbiola, B., Guerrero, J. E., Coll, N. Ramon, Jiménez, G. Jiménez, Calero, J. Codina, García, M., de la Torre, M. C., Vendrell, E., Palomera, E., Güell, E., Serra-Prat, M., Bermejo-Martín, J. F., Almirall, J., Tomas, E., Escoval, A., Froe, F., Pereira, M. H. Vitoria, Velez, N., Viegas, E., Filipe, E., Groves, C., Reay, M., Ballin, A., Facchin, F., Sartori, G., Zarantonello, F., Campello, E., Radu, C. M., Rossi, S., Ori, C., Simioni, P., Umei, N., Shingo, I., Santos, A. C., Candeias, C., Moniz, I., Marçal, R., e Silva, Z. Costa, Ribeiro, J. M., Georger, J. F., Ponthus, J. P., Tchir, M., Amilien, V., Ayoub, M., Barsam, E., Martucci, G., Panarello, G., Tuzzolino, F., Capitanio, G., Ferrazza, V., Carollo, T., Giovanni, L., Arcadipane, A., Sánchez, M. López, González-Gay, M. A., Díaz, F. J. Llorca, López, M. I. Rubio, Zogheib, E., Villeret, L., Nader, J., Bernasinski, M., Besserve, P., Caus, T., Dupont, H., Morimont, P., Habran, S., Hubert, R., Desaive, T., Blaffart, F., Janssen, N., Guiot, J., Pironet, A., Dauby, P., Lambermont, B., Pettenuzzo, T., Citton, G., Kirakli, C., Ediboglu, O., Ataman, S., Yarici, M., Tuksavul, F., Keating, S., Gibson, A., Gilles, M., Dunn, M., Price, G., Young, N., Remeta, P., Bishop, P., Zamora, M. D. Fernández, Muñoz-Bono, J., Curiel-Balsera, E., Aguilar-Alonso, E., Hinojosa, R., Gordillo-Brenes, A., Arboleda-Sánchez, J. A., Skorniakov, I., Vikulova, D., Whiteley, C., Shaikh, O., Jones, A., Ostermann, M., Forni, L., Scott, M., Sahatjian, J., Linde-Zwirble, W., Hansell, D., Laoveeravat, P., Srisawat, N., Kongwibulwut, M., Peerapornrattana, S., Suwachittanont, N., Wirotwan, T. O., Chatkaew, P., Saeyub, P., Latthaprecha, K., Tiranathanagul, K., Eiam-ong, S., Kellum, J. A., Berthelsen, R. E., Perner, A., Jensen, A. E. K., Jensen, J. U., Gebhard, D. J., Price, J., Kennedy, C. E., Akcan-Arikan, A., Kang, Y. R., Nakamae, M. N., Hamed, K., Khaled, M. M., Soliman, R. Aly, Mokhtar, M. Sherif, Seller-Pérez, G., Arias-Verdú, D., Llopar-Valdor, E., De-Diós-Chacón, I., Quesada-García, G., Herrera-Gutierrez, M. E., Hafes, R., Carroll, G., Doherty, P., Wright, C., Vera, I. G. Guerra, Ralston, M., Gemmell, M. L., MacKay, A., Black, E., Docking, R. I., Appleton, R., Ralston, M. R., Gemmell, L., Mackay, A., Röttgering, J. G., Elbers, P. W. G., Mejeni, N., Nsiala, J., Kilembe, A., Akilimali, P., Thomas, G., Andersson, A. E., Fagerdahl, A. M., Knudsen, V., Cheikh, A. Ben, Hamdaoui, Y., Guiga, A., Fraj, N., Sma, N., Chouchene, I., Bouafia, N., Amirian, A., Ziaian, B., Fleischmann, C., Thomas-Rueddel, D. O., Schettler, A., Schwarzkopf, D., Stacke, A., Reinhart, K., Martins, A., Sousa, P., Snell, G., Matsa, R., Paary, T. T. S., Cavalheiro, A. M., Rocha, L. L., Vallone, C. S., Tonilo, A., Lobato, M. D. S., Malheiro, D. T., Sussumo, G., Lucino, N. M., Rosenthal, V. D., Dashti, A. Sanaei, Yousefipour, A., Goodall, J. R., Williamson, M., Tant, E., Thomas, N., Balci, C., Gonen, C., Haftacı, E., Gurarda, H., Karaca, E., Paldusová, B., Zýková, I., Šímová, D., Houston, S., D’Antona, L., Lloyd, J., Garnelo-Rey, V., Sosic, M., Sotosek-Tokmazic, V., Kuharic, J., Antoncic, I., Dunatov, S., Sustic, A., Chong, C. T., Sim, M., Lyovarin, T., Díaz, F. M. Acosta, Galdó, S. Narbona, Garach, M. Muñoz, Romero, O. Moreno, Bailón, A. M. Pérez, Pinel, A. Carranza, Colmenero, M., Gritsan, A., Gazenkampf, A., Korchagin, E., Dovbish, N., Lee, R. M., Lim, M. P. P., Lim, B. C. L., See, J. J., Assis, R., Filipe, F., Lopes, N., Pessoa, L., Pereira, T., Catorze, N., Aydogan, M. S., Aldasoro, C., Marchio, P., Jorda, A., Mauricio, M. D., Guerra-Ojeda, S., Gimeno-Raga, M., Colque-Cano, M., Bertomeu-Artecero, A., Aldasoro, M., Valles, S. L., Tonon, D., Triglia, T., Martin, J. C., Alessi, M. C., Bruder, N., Garrigue, P., Velly, L., Spina, S., Scaravilli, V., Marzorati, C., Colombo, E., Savo, D., Vargiolu, A., Cavenaghi, G., Citerio, G., Andrade, A. H. V., Bulgarelli, P., Araujo, J. A. P., Gonzalez, V., Souza, V. A., Massant, C., Filho, C. A. C. Abreu, Morbeck, R. A., Burgo, L. E., van Groenendael, R., van Eijk, L. T., Leijte, G. P., Koeneman, B., Kox, M., Pickkers, P., García-de la Torre, A., de la Torre-Prados, M., Fernández-Porcel, A., Rueda-Molina, C., Nuevo-Ortega, P., Tsvetanova-Spasova, T., Cámara-Sola, E., García-Alcántara, A., Salido-Díaz, L., Liao, X., Feng, T., Zhang, J., Cao, X., Wu, Q., Xie, Z., Li, H., Kang, Y., Winkler, M. S., Nierhaus, A., Mudersbach, E., Bauer, A., Robbe, L., Zahrte, C., Schwedhelm, E., Kluge, S., Zöllner, C., Mitsi, E., Pennington, S. H., Reine, J., Wright, A. D., Parker, R., Welters, I. D., Blakey, J. D., Rajam, G., Ades, E. W., Ferreira, D. M., Wang, D., Kadioglu, A., Gordon, S. B., Koch, R., Rahamat-Langedoen, J., Schloesser, J., de Jonge, M., Bringue, J., Guillamat-Prats, R., Torrents, E., Martinez, M. L., Camprubí-Rimblas, M., Blanch, L., Park, S. Y., Park, Y. B., Song, D. K., Shrestha, S., Park, S. H., Koh, Y., Park, M. J., Hong, C. W., Lesur, O., Coquerel, D., Sainsily, X., Cote, J., Söllradl, T., Murza, A., Dumont, L., Dumaine, R., Grandbois, M., Sarret, P., Marsault, E., Salvail, D., Auger-Messier, M., Chagnon, F., Lauretta, M. P., Greco, E., Dyson, A., Preau, S., Ambler, M., Sigurta, A., Saeed, S., Sarıca, L. Topcu, Zibandeh, N., Genc, D., Gul, F., Akkoc, T., Kombak, E., Cinel, L., Cinel, I., Pollen, S. J., Arulkumaran, N., Warnes, G., Pennington, D. J., Brohi, K., O’Dwyer, M. J., Kim, H. Y., Na, S., Kim, J., Chang, Y. F., Chao, A., Shih, P. Y., Lee, C. T., Yeh, Y. C., Chen, L. W., Adriaanse, M., Rietdijk, W., Funcke, S., Sauerlaender, S., Saugel, B., Pinnschmidt, H., Reuter, D. A., Nitzschke, R., Perbet, S., Biboulet, C., Lenoire, A., Bourdeaux, D., Pereira, B., Plaud, B., Bazin, J. E., Sautou, V., Mebazaa, A., Constantin, J. M., Legrand, M., Boyko, Y., Jennum, P., Nikolic, M., Oerding, H., Holst, R., Toft, P., Nedergaard, H. K., Haberlandt, T., Park, S., Kim, S., Cho, Y. J., Lim, Y. J., Chan, A., Tang, S., Nunes, S. L., Forsberg, S., Blomqvist, H., Berggren, L., Sörberg, M., Sarapohja, T., Wickerts, C. J., Hofhuis, J. G. M., Rose, L., Blackwood, B., Akerman, E., Mcgaughey, J., Fossum, M., Foss, H., Georgiou, E., Graff, H. J., Kalafati, M., Sperlinga, R., Schafer, A., Wojnicka, A. G., Spronk, P. E., Khalili, F., Afshari, R., Khodaei, H. Haddad, Javadpour, S., Petramfar, P., Nasimi, S., Tabei, H., Gunther, A., Hansen, J. O., Sackey, P., Storm, H., Bernhardsson, J., Sundin, Ø., Bjärtå, A., Bienert, A., Smuszkiewicz, P., Wiczling, P., Przybylowski, K., Borsuk, A., Trojanowska, I., Matysiak, J., Kokot, Z., Paterska, M., Grzeskowiak, E., Messina, A., Bonicolini, E., Colombo, D., Moro, G., Romagnoli, S., De Gaudio, A. R., Corte, F. Della, Romano, S. M., Silversides, J. A., Major, E., Mann, E. E., Ferguson, A. J., Mcauley, D. F., Marshall, J. C., Diaz-Rodriguez, J. A., Silva-Medina, R., Gomez-Sandoval, E., Gomez-Gonzalez, N., Soriano-Orozco, R., Gonzalez-Carrillo, P. L., Hernández-Flores, M., Pilarczyk, K., Lubarksi, J., Wendt, D., Dusse, F., Günter, J., Huschens, B., Demircioglu, E., Jakob, H., Palmaccio, A., Dell’Anna, A. M., Grieco, D. L., Torrini, F., Iaquaniello, C., Bongiovanni, F., Antonelli, M., Toscani, L., Antonakaki, D., Bastoni, D., Jozwiak, M., Depret, F., Teboul, J. L., Alphonsine, J., Lai, C., Richard, C., Monnet, X., Demeter, G., Kertmegi, I., Hasanin, A., Lotfy, A., El-adawy, A., Nassar, H., Mahmoud, S., Abougabal, A., Mukhtar, A., Quinty, F., Habchi, S., Luzi, A., Antok, E., Hernandez, G., Lara, B., Enberg, L., Ortega, M., Leon, P., Kripper, C., Aguilera, P., Kattan, E., Lehmann, M., Sakka, S., Bein, B., Schmid, R. M., Preti, J., Creteur, J., Herpain, A., Marc, J., Trojette, F., Bar, S., Kontar, L., Titeca, D., Richecoeur, J., Gelee, B., Verrier, N., Mercier, R., Lorne, E., Maizel, J., Slama, M., Abdelfattah, M. E., Eladawy, A., Elsayed, M. A. Ali, Montenegro, A. Pedraza, Zepeda, E. Monares, Granillo, J. Franco, Sánchez, J. S. Aguirre, Alejo, G. Camarena, Cabrera, A. Rugerio, Montoya, A. A. Tanaka, Lee, C., Hatib, F., Cannesson, M., Theerawit, P., Morasert, T., Sutherasan, Y., Zani, G., Mescolini, S., Diamanti, M., Righetti, R., Scaramuzza, A., Papetti, M., Terenzoni, M., Gecele, C., Fusari, M., Hakim, K. A., Chaari, A., Ismail, M., Elsaka, A. H., Mahmoud, T. M., Bousselmi, K., Kauts, V., Casey, W. F., Hutchings, S. D., Naumann, D., Wendon, J., Watts, S., Kirkman, E., Jian, Z., Buddi, S., Settels, J., Bertini, P., Guarracino, F., Trepte, C., Richter, P., Haas, S. A., Eichhorn, V., Kubitz, J. C., Soliman, M. S., Hamimy, W. I., Fouad, A. Z., Mukhtar, A. M., Charlton, M., Tonks, L., Mclelland, L., Coats, T. J., Thompson, J. P., Sims, M. R., Williams, D., Roushdy, D. Z., Soliman, R. A., Nahas, R. A., Arafa, M. Y., Hung, W. T., Chiang, C. C., Huang, W. C., Lin, K. C., Lin, S. C., Cheng, C. C., Kang, P. L., Wann, S. R., Mar, G. Y., Liu, C. P., Carranza, M. Lopez, Fernandez, H. Sancho, Roman, J. A. Sanchez, Lucena, F., Garcia, A. Campanario, Vazquez, A. Loza, Serrano, A. Lesmes, Moreira, L. Sayagues, Vidal-Perez, R., Herranz, U. Anido, Acuna, J. M. Garcia, Gil, C. Pena, Allut, J. L. Garcia, Sedes, P. Rascado, Lopez, C. Martin, Paz, E. Saborido, Rodriguez, C. Galban, Gonzalez-Juanatey, J. R., Vallejo-Baez, A., de la Torre-Prados, M. V., Marharaj, R., Gervasio, K., Bottiroli, M., Mondino, M., De Caria, D., Calini, A., Montrasio, E., Milazzo, F., Gagliardone, M. P., Vallejo-Báez, A., Anido, U., Cheikh-Bouhlel, M., Dela Cruz, M. P. R. D. L., Bernardo, J. M., Galfo, F., Marino, A., Chao, C. C., Hou, P., Hung, C. C., Chiang, C. H., Liou, Y. J., Hung, S. M., Lin, Y. S., Kuo, F. Y., Chiou, K. R., Chen, C. J., Yan, L. S., Liu, C. Y., Wang, H. H., Chen, H. L., Ho, C. K., Grewal, S., Gopal, S., Corbett, C., Wilson, A., Capps, J., Ayoub, W., Lomas, A., Ghani, S., Moore, J., Atkinson, D., Sharman, M., Swinnen, W., Pauwels, J., Mignolet, K., Pannier, E., Koch, A., Sarens, T., Temmerman, W., Elmenshawy, A. M., Fayed, A. M., Elboriuny, M., Hamdy, E., Zakaria, E., Falk, A. C., Petosic, A., Olafsen, K., Wøien, H., Flaatten, H., Sunde, K., Agra, J. J. Cáceres, Cabrera, J. L. Santana, Santana, J. D. Martín, Alzola, L. Melián, Pérez, H. Rodríguez, Pires, T. Castro, Calderón, H., Pereira, A., Castro, S., Granja, C., Norkiene, I., Urbanaviciute, I., Kezyte, G., Ringaitiene, D., Jovaisa, T., Vogel, G., Johansson, U. B., Sandgren, A., Svensen, C., Joelsson-Alm, E., Leite, M. A., Murbach, L. D., Osaku, E. F., Costa, C. R. L. M., Pelenz, M., Neitzke, N. M., Moraes, M. M., Jaskowiak, J. L., Silva, M. M. M., Zaponi, R. S., Abentroth, L. R. L., Ogasawara, S. M., Jorge, A. C., Duarte, P. A. D., Barreto, J., Duarte, S. T., Taba, S., Miglioranza, D., Gund, D. P., Lordani, C. F., Vollmer, H., Gager, M., Waldmann, C., Mazzeo, A. T., Tesio, R., Filippini, C., Vallero, M. E., Giolitti, C., Caccia, S., Medugno, M., Tenaglia, T., Rosato, R., Mastromauro, I., Brazzi, L., Terragni, P. P., Urbino, R., Fanelli, V., Ranieri, V. M., Mascia, L., Ballantyne, J., Paton, L., Perez-Teran, P., Roca, O., Ruiz-Rodriguez, J. C., Zapatero, A., Serra, J., Bianzina, S., Cornara, P., Rodi, G., Tavazzi, G., Pozzi, M., Iotti, G. A., Mojoli, F., Braschi, A., Vishnu, A., Buche, D., Pande, R., Moolenaar, D. L. J., Bakhshi-Raiez, F., Dongelmans, D. A., de Keizer, N. F., de Lange, D. W., Fernández, I. Fuentes, Baño, D. Martínez, Moreno, J. L. Buendía, Rubio, R. Jara, Scott, J., Phelan, D., Morely, D., O’Flynn, J., Stapleton, P., Lynch, M., Marsh, B., Carton, E., O’Loughlin, C., Cheng, K. C., Sung, M. I., Elghonemi, M. O., Saleh, M. H., Meyhoff, T. S., Krag, M., Hjortrup, P. B., Møller, M. H., Öhman, T., Sigmundsson, T., Redondo, E., Hallbäck, M., Suarez-Sipmann, F., Björne, H., Sander, C. Hällsjö, Chiumello, D., Chiurazzi, C., Brioni, M., Algieri, I., Guanziroli, M., Vergani, G., Tonetti, T., Tomic, I., Colombo, A., Crimella, F., Carlesso, E., Gasparovic, V., El-Sherif, R., Al-Basser, M. Abd, Raafat, A., El-Sherif, A., Schouten, L. R. A., Cremer, O. L., Ong, D. S. Y., Amoruso, G., Cinnella, G., Bos, L. D. J., Schmidle, P., Findeisen, M., Hoppmann, P., Jaitner, J., Brettner, F., Lahmer, T., Rajagopalan, G., Bansal, V., Frank, R., Hinds, R., Levitt, J., Siddiqui, S., Gilbert, J. P., Sim, K., Wang, C. H., Li, I. J., Tang, W. R., Persona, P., De Cassai, A., Franco, M., Goffi, A., Ruiz, B. Llorente, Varas, J. Lujan, Montero, R. Molina, Delgado, C. Pintado, Navarrete, O., Mezquita, M. Vazquez, Peces, E. Alonso, Nakamura, M. A. M., Hajjar, L. A., Galas, F. R. B. G., Ortiz, T. A., Amato, M. B. P., Bitker, L., Costes, N., Le Bars, D., Lavenne, F., Mojgan, D., Richard, J. C., Massari, D., Gotti, M., Cadringher, P., Zerman, A., Türkoğlu, M., Arık, G., Yıldırım, F., Güllü, Z., Kara, I., Boyacı, N., Aydoğan, B. Basarık, Gaygısız, Ü., Gönderen, K., Aygencel, G., Aydoğdu, M., Ülger, Z., Gürsel, G., Riera, J., Toral, C. Maldonado, Mazo, C., Martínez, M., Baldirà, J., Lagunes, L., Roman, A., Deu, M., Rello, J., Levine, D. J., Mohus, R. M., Askim, Å., Paulsen, J., Mehl, A., Dewan, A. T., Damås, J. K., Solligård, E., Åsvold, B. O., DeWan, A., Aktepe, O., Kara, A., Yeter, H., Topeli, A., Norrenberg, M., Devroey, M., Khader, H., Preiser, J. C., Tang, Z., Qiu, C., Tong, L., Cai, C., Apostolopoulou, O., Moon, J. Y., Park, M. R., Kwon, I. S., Chon, G. R., Ahn, J. Y., Kwon, S. J., Chang, Y. J., Lee, J. Y., Yoon, S. Y., Lee, J. W., Kostalas, M., Mckinlay, J., Kooner, G., Dudas, G., Horton, A., Kerr, C., Karanjia, N., Creagh-Brown, B., Altintas, N. D., Izdes, S., Keremoglu, O., Alkan, A., Neselioglu, S., Erel, O., Tardif, N., Gustafsson, T., MacEachern, K. N., Traille, M., Bromberg, I., Lapinsky, S. E., Moore, M. J., García-Garmendia, J. L., Villarrasa-Clemente, F., Maroto-Monserrat, F., Rufo-Tejeiro, O., Jorge-Amigo, V., Sánchez-Santamaría, M., Colón-Pallarés, C., Barrero-Almodóvar, A., Gallego-Lara, S., Anthon, C. T., Müller, R. B., Haase, N., Møller, K., Wetterslev, J., Nakanishi, M., Kuriyama, A., Fukuoka, T., Abd el Halim, M. A., Elsaid hafez, M. H., Moktar, A. M., Elazizy, H. M., Hakim, K. Abdel, Elbahr, M., Mahmoud, T., Khalil, E., Casey, W., Zaky, S. H., Rizk, A., Ahmed, R., Ospina-Tascón, G. A., Marin, A. F. Garcia, Echeverry, G. J., Bermudez, W. F., Madriñan-Navia, H. J., Valencia, J. D., Quiñonez, E., Marulanda, A., Arango-Dávila, C. A., Bruhn, A., De Backer, D., Cortes, D. Orbegozo, Su, F., Vincent, J. L., Tullo, L., Mirabella, L., Di Molfetta, P., Dambrosio, M., Lujan, C. Villavicencio, irigoyen, J. Leache, Cartanya ferré, M., García, R. Carbonell, Ahmed, M., El Ayashi, M., Ayman, E., Salem, M., Fathy, S., Zaghlol, A., Arzapalo, M. F. Aguilar, Valsø, Å., Rustøen, T., Schou-Bredal, I., Skogstad, L., Tøien, K., Padilla, C., Palmeiro, Y., Egbaria, W., Kigli, R., Maertens, B., Blot, K., Blot, S., Santana-Santos, E., dos Santos, E. R., Ferretti-Rebustini, R. E. D. L., dos Santos, R. D. C. C. D. O., Verardino, R. G. S., Bortolotto, L. A., Doyle, A. M., Naldrett, I., Tillman, J., Price, S., Pearson, P., Greaves, J., Goodall, D., Berry, A., Richardson, A., Odundo, G. O., Omengo, P., Obonyo, P., Chanzu, N. M., Kleinpell, R., Sarris, S. J., Nedved, P., Heitschmidt, M., Ben-Ghezala, H., Snouda, S., Djobbi, S., Adhikari, N. K. J., Leasa, D., Fergusson, D., Mckim, D. A., Weblin, J., McWilliams, D., Doesburg, F., Cnossen, F., Dieperink, W., Bult, W., Nijsten, M. W. N., Galvez-Blanco, G. A., Guzman, C. I. Olvera, Stroud, J. Santos, Thomson, R., Llaurado-Serra, M., Lobo-Civico, A., Pi-Guerrero, M., Blanco-Sanchez, I., Piñol-Tena, A., Paños-Espinosa, C., Alabart-Segura, Y., Coloma-Gomez, B., Fernandez-Blanco, A., Braga-Dias, F., Treso-Geira, M., Valeiras-Valero, A., Martinez-Reyes, L., Sandiumenge, A., Jimenez-Herrera, M. F., Prada, R., Juárez, P., Argandoña, R., Díaz, J. J., Ramirez, C. Sánchez, Saavedra, P., Santana, S. Ruiz, Obukhova, O., Kashiya, S., Kurmukov, I. A., Pronina, A. M., Simeone, P., Puybasset, L., Auzias, G., Coulon, O., Lesimple, B., Torkomian, G., Bartkowska-Sniatkowska, A., Szerkus, O., Siluk, D., Bartkowiak-Wieczorek, J., Rosada-Kurasinska, J., Warzybok, J., Kaliszan, R., Caballero, C. Hernandez, Roberts, S., Isgro, G., Hall, D., Guillaume, G., Passouant, O., Dumas, F., Bougouin, W., Champigneulle, B., Arnaout, M., Chelly, J., Chiche, J. D., Varenne, O., Mira, J. P., Marijon, E., Cariou, A., Beerepoot, M., Touw, H. R., Parlevliet, K., Boer, C., Elbers, P. W., Reina, Á. J. Roldán, Palomo, Y. Corcia, Bermúdez, R. Martín, Villén, L. Martín, García, I. Palacios, Izurieta, J. R. Naranjo, Bernal, J. B. Pérez, Jiménez, F. J. Jiménez, Cota-Delgado, F., Kaneko, T., Tanaka, H., Kamikawa, M., Karashima, R., Iwashita, S., Irie, H., Kasaoka, S., Arola, O., Laitio, R., Saraste, A., Airaksinen, J., Pietilä, M., Hynninen, M., Wennervirta, J., Bäcklund, M., Ylikoski, E., Silvasti, P., Nukarinen, E., Grönlund, J., Harjola, V. P., Niiranen, J., Korpi, K., Varpula, M., Roine, R. O., Laitio, T., Salah, S., Hassen, B. G., Fehmi, A. Mohamed, Hsu, Y. C., Barea-Mendoza, J., García-Fuentes, C., Castillo-Jaramillo, M., Dominguez-Aguado, H., Viejo-Moreno, R., Terceros-Almanza, L., Aznárez, S. Bermejo, Mudarra-Reche, C., Xu, W., Chico-Fernández, M., Montejo-González, J. C., Crewdson, K., Thomas, M., Merghani, M., Fenner, L., Morgan, P., Lockey, D., van Lieshout, E. J., Oomen, B., Binnekade, J. M., de Haan, R. J., Juffermans, N. P., Vroom, M. B., Algarte, R., Martínez, L., Sánchez, B., Romero, I., Martínez, F., Quintana, S., Trenado, J., Sheikh, O., Pogson, D., Clinton, R., Riccio, F., Arthur, A., Young, L., Sinclair, A., Markopoulou, D., Venetsanou, K., Filippou, L., Salla, E., Stratouli, S., Alamanos, I., Guirgis, A. H., Rodriguez, R. Gutiérrez, Lorente, M. J. Furones, Guarasa, I. Macias, Ukere, A., Meisner, S., Greiwe, G., Opitz, B., Benten, D., Nashan, B., Fischer, L., Trepte, C. J. C., Behem, C. R., Ana, B., Vazir, A., Gibson, D., Hadavi, M. R., alam, M. Riahi, Sasani, M. R., Parenti, N., Agrusta, F., Palazzi, C., Pifferi, B., Sganzerla, R., Tagliazucchi, F., Luciani, A., Möller, M., Müller-Engelmann, J., Montag, G., Adams, P., Lange, C., Neuzner, J., Gradaus, R., Wodack, K. H., Thürk, F., Waldmann, A. D., Grässler, M. F., Nishimoto, S., Böhm, S. H., Kaniusas, E., Trepte, C. J., Wallin, M., Sipman, F. Suarez, Oldner, A., Colinas, L., Vicho, R., Serna, M., Cuena, R., Canabal, A., Etman, M., El Bahr, M., El Sakka, A., Arali, A., Bond, O., De Santis, P., Iesu, E., Franchi, F., Scolletta, S., Taccone, F. S., Marutyan, Z., Hamidova, L., Shakotko, A., Movsisyan, V., Uysupova, I., Evdokimov, A., Petrikov, S., Calvo, F. J. Redondo, Bejarano, N., Baladron, V., Villazala, R., Redondo, J., Padilla, D., Villarejo, P., Gomez-Gonzalez, C., Mas-Font, S., Puppo-Moreno, A., Herrera-Gutierrez, M., Garcia-Garcia, M., Aldunate-Calvo, S., Plata-Menchaca, E. P., Pérez-Fernández, X. L., Estruch, M., Betbese-Roig, A., Campos, P. Cárdenas, Lora, M. Rojas, Gaibor, N. D. Toapanta, Medina, R. S. Contreras, Sanguino, V. D. Gumucio, Casanova, E. J., Riera, J. Sabater, Kritmetapak, K., Peerapornratana, S., Kittiskulnam, P., Dissayabutra, T., Susantithapong, P., Praditpornsilpa, K., Tungsanga, K., Eiam-Ong, S., Winkelmann, T., Busch, T., Meixensberger, J., Bercker, S., Cabeza, E. M. Flores, Sánchez, M. Sánchez, Giménez, N. Cáceres, Melón, C. Gutierrez, de Lucas, E. Herrero, Estañ, P. Millán, Bernal, M. Hernández, de Lorenzo y Mateos, A. Garcia, Specht, P. A. C., Balik, M., Zakharchenko, M., Los, F., Brodska, H., de Tymowski, C., Augustin, P., Desmard, M., Montravers, P., Stapel, S. N., de Boer, R., Oudemans, H. M., Hollinger, A., Schweingruber, T., Jockers, F., Dickenmann, M., Siegemund, M., Runciman, N., Alban, L., Turrini, C., Sasso, T., Langer, T., Taccone, P., Marenghi, C., Grasselli, G., Wibart, P., Reginault, T., Garcia, M., Barbrel, B., Benard, A., Bader, C., Vargas, F., Bui, H. N., Hilbert, G., Simón, J. M. Serrano, Sánchez, P. Carmona, Ferrón, F. Ruiz, de Acilu, M. García, Marin, J., Antonia, V., Ruano, L., Monica, M., Hong, G., Kim, D. H., Kim, Y. S., Park, J. S., Jee, Y. K., xiang, Z. Yu, Jia-xing, W., dan, W. Xiao, long, N. Wen, Yu, W., Yan, Z., Cheng, X., Kobayashi, T., Onodera, Y., Akimoto, R., Sugiura, A., Suzuki, H., Iwabuchi, M., Nakane, M., Kawamae, K., Sanchez, P. Carmona, Rodriguez, M. D. Bautista, Delgado, M. Rodriguez, Sánchez, V. Martínez de Pinillos, Gómez, A. Mula, Beuret, P., Fortes, C., Lauer, M., Reboul, M., Chakarian, J. C., Fabre, X., Philippon-Jouve, B., Devillez, S., Clerc, M., Rittayamai, N., Sklar, M., Dres, M., Rauseo, M., Campbell, C., West, B., Tullis, D. E., Okada, M., Ahmad, N., Wood, M., Glossop, A., Lucas, J. Higuera, Ortiz, A. Blandino, Alonso, D. Cabestrero, De Pablo Sánchez, R., González, L. Rey, Costa, R., Spinazzola, G., Pizza, A., Ferrone, G., Rossi, M., Conti, G., Ribeiro, H., Alves, J., Sousa, M., Reis, P., Socolovsky, C. S., Cauley, R. P., Frankel, J. E., Beam, A. L., Olaniran, K. O., Gibbons, F. K., Christopher, K. B., Pennington, J., Zolfaghari, P., King, H. S., Kong, H. H. Y., Shum, H. P., Yan, W. W., Kaymak, C., Okumus, N., Sari, A., Erdogdu, B., Aksun, S., Basar, H., Ozcan, A., Ozcan, N., Oztuna, D., Malmgren, J. A., Lundin, S., Torén, K., Eckerström, M., Wallin, A., Waldenström, A. C., Riccio, F. C., Antonio, A. C. P., Leivas, A. F., Kenji, F., James, E., Jonnada, S., Gerrard, C. S., Jones, N., Salciccioli, J. D., Marshall, D. C., Komorowski, M., Hartley, A., Sykes, M. C., Goodson, R., Shalhoub, J., Villanueva, J. R. Fernández, Garda, R. Fernández, Lago, A. M. López, Ruiz, E. Rodríguez, Vaquero, R. Hernández, Rodríguez, C. Galbán, Pérez, E. Varo, Hilasque, C., Oliva, I., Sirgo, G., Martin, M. C., Olona, M., Gilavert, M. C., Bodí, M., Ebm, C., Aggarwal, G., Huddart, S., Quiney, N., Fernandes, S. M., Silva, J. Santos, Gouveia, J., Silva, D., Marques, R., Bento, H., Alvarez, A., Silva, Z. Costa, Diaz, D. Díaz, Martínez, M. Villanova, Herrejon, E. Palencia, de la Gandara, A. Martinez, Gonzalo, G., Lopez, M. A., de Gopegui Miguelena, P. Ruíz, Matilla, C. I. Bernal, Chueca, P. Sánchez, Longares, M. D. C. Rodríguez, Abril, R. Ramos, Aguilar, A. L. Ruíz, de Murillas, R. Garrido López, Fernández, R. Fernández, Laborías, P. Morales, Castellanos, M. A. Díaz, Laborías, M. E. Morales, Park, J., Woo, S., West, T., Powell, E., Rimmer, A., Orford, C., Williams, J., de Gopegui Miguelena, P. Ruiz, Bourne, R. S., Shulman, R., Tomlin, M., Mills, G. H., Borthwick, M., Berry, W., Huertas, D. García, Manzano, F., Villagrán-Ramírez, F., Ruiz-Perea, A., Rodríguez-Mejías, C., Santiago-Ruiz, F., Colmenero-Ruiz, M., König, C., Matt, B., Kortgen, A., Hartog, C. S., Wong, A., Balan, C., Barker, G., Tachaboon, S., Paratz, J., Kayambu, G., Boots, R., Vlasenko, R., Gromova, E., Loginov, S., Kiselevskiy, M., Dolgikova, Y., Tang, K. B., Chau, C. M., Lam, K. N., Gil, E., Suh, G. Y., Park, C. M., Chung, C. R., Lai, C. H., Cheng, Y. J., Colella, V., Zarrillo, N., D’Amico, M., Forfori, F., Pezza, B., Laddomada, T., Beltramelli, V., Pizzaballa, M. L., Doronzio, A., Balicco, B., Kiers, D., van der Heijden, W., Gerretsen, J., de Mast, Q., el Messaoudi, S., Rongen, G., Gomes, M., Riksen, N. P., Kashiwagi, Y., Hayashi, K., Inagaki, Y., Fujita, S., Blet, A., Sadoune, M., Lemarié, J., Bihry, N., Bern, R., Polidano, E., Merval, R., Launay, J. M., Lévy, B., Samuel, J. L., Hartmann, J., Harm, S., and Weber, V.

Published
2016
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11. LysM receptor-like kinases involved in immunity perceive lipo-chitooligosaccharides in mycotrophic plants.

Author

Wang T, Gasciolli V, Gaston M, Medioni L, Cumener M, Buendia L, Yang B, Bono JJ, He G, and Lefebvre B

Subjects
Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Chitin metabolism, Symbiosis physiology, Plants metabolism, Plant Roots metabolism, Mycorrhizae physiology, Chitosan metabolism
Abstract

Symbiotic microorganisms such as arbuscular mycorrhizal fungi (AMF) produce both conserved microbial molecules that activate plant defense and lipo-chitooligosaccharides (LCOs) that modulate plant defense. Beside a well-established role of LCOs in the activation of a signaling pathway required for AMF penetration in roots, LCO perception and defense modulation during arbuscular mycorrhiza is not well understood. Here we show that members of the LYRIIIA phylogenetic group from the multigenic Lysin Motif Receptor-Like Kinase family have a conserved role in dicotyledons as modulators of plant defense and regulate AMF colonization in the Solanaceae species Nicotiana benthamiana. Interestingly, these proteins have a high-affinity for LCOs in plant species able to form a symbiosis with AMF but have lost this property in species that have lost this ability. Our data support the hypothesis that LYRIIIA proteins modulate plant defense upon LCO perception to facilitate AMF colonization in mycotrophic plant species and that only their role in plant defense, but not their ability to be regulated by LCOs, has been conserved in non-mycotrophic plants., Competing Interests: Conflict of interest statement. None declared., (© American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2023
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12. Plant neighbour-modulated susceptibility to pathogens in intraspecific mixtures.

Author

Pélissier R, Buendia L, Brousse A, Temple C, Ballini E, Fort F, Violle C, and Morel JB

Subjects
Genotype, Oryza genetics, Triticum genetics
Abstract

As part of a trend towards diversifying cultivated areas, varietal mixtures are subject to renewed interest as a means to manage diseases. Besides the epidemiological effects of varietal mixtures on pathogen propagation, little is known about the effect of intraspecific plant-plant interactions and their impact on responses to disease. In this study, genotypes of rice (Oryza sativa) or durum wheat (Triticum turgidum) were grown with different conspecific neighbours and manually inoculated under conditions preventing pathogen propagation. Disease susceptibility was measured together with the expression of basal immunity genes as part of the response to intra-specific neighbours. The results showed that in many cases for both rice and wheat susceptibility to pathogens and immunity was modified by the presence of intraspecific neighbours. This phenomenon, which we term 'neighbour-modulated susceptibility' (NMS), could be caused by the production of below-ground signals and does not require the neighbours to be infected. Our results suggest that the mechanisms responsible for reducing disease in varietal mixtures in the field need to be re-examined., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.)

Published
2021
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13. Cardiovascular Risk Factors in Younger Black Women: Results from the 10,000 Women Community Screening Project.

Author

Vatsa N, Thobani A, Buendia L, Murphy K, Asier S, Chen Z, Ko YA, Putnam T, Mehta PK, and Lundberg GP

Abstract

Background: Cardiovascular Disease (CVD) risk factors are prevalent in black women, but when these risk factors arise is not clear. We aimed to determine when obesity, hypertension, and hyperlipidemia appear in black women within a community screening program., Methods: 945 black women who enrolled in the 10,000 Women community screening project in the metro Atlanta area were included (2015-2018). Socioeconomic, lifestyle, and traditional CVD risk factor information was patient-reported and measured. Characteristics of three cohorts stratified by age, 20-39 years old (yo), 40-59 yo, and ≥60 yo, were compared using pairwise analysis., Results: All cohorts had class 1 obesity. Mean systolic blood pressure was higher in older cohorts [20-39 yo: 122 ± 15; 40-59 yo: 133 ± 19; ≥60 yo: 142 ± 20 mmHg; p  < 0.001]. All age groups had mean total cholesterol levels below 200 mg/dL and were lowest in women 20-39 yo, ( p  < 0.001). All age groups had mean LDL levels below 100 mg/dL and were highest in women 20-39 yo, ( p  < 0.01). All age groups had mean HDL levels greater than 50 mg/dL and were highest in women ≥60 yo, ( p -value = 0.03). A higher proportion of ≥60 yo limited salt intake, ( p  ≤ 0.001), and ate fast food less than three times a week, ( p  < 0.001), compared to younger women., Conclusion: We report that CVD risk factors, like elevated blood pressure and obesity, are prevalent at young ages in black women, which could be due to lifestyle practices. Earlier initiation of CVD preventive care in black women could be beneficial; however, this needs to be studied further., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Table 1 Frequency and percent of baseline characteristics of the sample (from top to bottom), including CVD lifestyle risk factors, social determinants of health, and traditional CVD risk factors. P-values from left to right include chi-square analyses and posthoc Bonferroni pairwise comparisons between age groups (adjusted significant p-value <0.0015). Table 2: CVD medical risk factor mean (SD) for the three 20-year age cohorts and total sample. P-values from left to right for measured BMI, blood pressure, and cholesterol include ANOVA comparison and posthoc Tukey pairwise comparisons between age groups adjusted for income and education (significant p-value <0.05)., (© 2021 The Authors.)

Published
2021
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14. Exploring physiological traits for measuring response to competition in durum wheat.

Author

Henry L, Morel J, and Buendia L

Subjects
Chromosomes, Plant genetics, Genome-Wide Association Study, Genotype, Kinetics, Triticum genetics
Abstract

Studies of the effect of plant density have been conducted in numerous crops. However, most of them focused on yield to evaluate the impact of plant competition. In this study, we determined the best functional trait to detect early competition responses affecting plant growth. To do so, we designed experiments with increasing sowing density using two different durum wheat genotypes. Height from crown to the last liguled leaf and dry weight decreased with increasing sowing density showing that these traits are suitable to measure early competition responses. However, specific leaf area, a common-measured trait to study competition was not affected by sowing density at early stages. We conclude that plant density is a modifier of plant growth at the early stages of plant growth and that dry weight as well as height from crown to the last liguled leaf are the best functional traits to be measured.

Published
2020
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15. LCO Receptors Involved in Arbuscular Mycorrhiza Are Functional for Rhizobia Perception in Legumes.

Author

Girardin A, Wang T, Ding Y, Keller J, Buendia L, Gaston M, Ribeyre C, Gasciolli V, Auriac MC, Vernié T, Bendahmane A, Ried MK, Parniske M, Morel P, Vandenbussche M, Schorderet M, Reinhardt D, Delaux PM, Bono JJ, and Lefebvre B

Subjects
Chitin analogs & derivatives, Chitin metabolism, Chitosan, Fabaceae metabolism, Fabaceae microbiology, Gene Expression Regulation, Plant genetics, Solanum lycopersicum metabolism, Mycorrhizae metabolism, Oligosaccharides, Petunia metabolism, Plant Proteins metabolism, Protein Kinases metabolism, Signal Transduction genetics, Symbiosis genetics, Lipopolysaccharides metabolism, Mycorrhizae physiology, Rhizobium metabolism
Abstract

Bacterial lipo-chitooligosaccharides (LCOs) are key mediators of the nitrogen-fixing root nodule symbiosis (RNS) in legumes. The isolation of LCOs from arbuscular mycorrhizal fungi suggested that LCOs are also signaling molecules in arbuscular mycorrhiza (AM). However, the corresponding plant receptors have remained uncharacterized. Here we show that petunia and tomato mutants in the LysM receptor-like kinases LYK10 are impaired in AM formation. Petunia and tomato LYK10 proteins have a high affinity for LCOs (Kd in the nM range) comparable to that previously reported for a legume LCO receptor essential for the RNS. Interestingly, the tomato and petunia LYK10 promoters, when introduced into a legume, were active in nodules similarly to the promoter of the legume orthologous gene. Moreover, tomato and petunia LYK10 coding sequences restored nodulation in legumes mutated in their orthologs. This combination of genetic and biochemical data clearly pinpoints Solanaceous LYK10 as part of an ancestral LCO perception system involved in AM establishment, which has been directly recruited during evolution of the RNS in legumes., (Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Published
2019
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16. Lipo-chitooligosaccharides promote lateral root formation and modify auxin homeostasis in Brachypodium distachyon.

Author

Buendia L, Maillet F, O'Connor D, van de-Kerkhove Q, Danoun S, Gough C, Lefebvre B, and Bensmihen S

Subjects
Brachypodium drug effects, Brachypodium genetics, Chitin pharmacology, Chitosan, Fluorescence, Indoles metabolism, Models, Biological, Oligosaccharides, Plant Roots drug effects, Signal Transduction drug effects, Brachypodium growth & development, Chitin analogs & derivatives, Homeostasis drug effects, Indoleacetic Acids pharmacology, Lipids pharmacology, Plant Roots growth & development
Abstract

Lipo-chitooligosaccharides (LCOs) are microbial symbiotic signals that also influence root growth. In Medicago truncatula, LCOs stimulate lateral root formation (LRF) synergistically with auxin. However, the molecular mechanisms of this phenomenon and whether it is restricted to legume plants are not known. We have addressed the capacity of the model monocot Brachypodium distachyon (Brachypodium) to respond to LCOs and auxin for LRF. For this, we used a combination of root phenotyping assays, live-imaging and auxin quantification, and analysed the regulation of auxin homeostasis genes. We show that LCOs and a low dose of the auxin precursor indole-3-butyric acid (IBA) stimulated LRF in Brachypodium, while a combination of LCOs and IBA led to different regulations. Both LCO and IBA treatments locally increased endogenous indole-3-acetic acid (IAA) content, whereas the combination of LCO and IBA locally increased the endogenous concentration of a conjugated form of IAA (IAA-Ala). LCOs, IBA and the combination differentially controlled expression of auxin homeostasis genes. These results demonstrate that LCOs are active on Brachypodium roots and stimulate LRF probably through regulation of auxin homeostasis. The interaction between LCO and auxin treatments observed in Brachypodium on root architecture opens interesting avenues regarding their possible combined effects during the arbuscular mycorrhizal symbiosis., (© 2018 The Authors New Phytologist © 2018 New Phytologist Trust.)

Published
2019
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17. Sunflower resistance to multiple downy mildew pathotypes revealed by recognition of conserved effectors of the oomycete Plasmopara halstedii.

Author

Pecrix Y, Buendia L, Penouilh-Suzette C, Maréchaux M, Legrand L, Bouchez O, Rengel D, Gouzy J, Cottret L, Vear F, and Godiard L

Subjects
Chromosome Mapping, Disease Resistance genetics, Disease Resistance physiology, Genotype, Virulence genetics, Virulence physiology, Helianthus metabolism, Helianthus microbiology, Oomycetes pathogenicity, Plant Diseases microbiology
Abstract

Over the last 40 years, new sunflower downy mildew isolates (Plasmopara halstedii) have overcome major gene resistances in sunflower, requiring the identification of additional and possibly more durable broad-spectrum resistances. Here, 354 RXLR effectors defined in silico from our new genomic data were classified in a network of 40 connected components sharing conserved protein domains. Among 205 RXLR effector genes encoding conserved proteins in 17 P. halstedii pathotypes of varying virulence, we selected 30 effectors that were expressed during plant infection as potentially essential genes to target broad-spectrum resistance in sunflower. The transient expression of the 30 core effectors in sunflower and in Nicotiana benthamiana leaves revealed a wide diversity of targeted subcellular compartments, including organelles not so far shown to be targeted by oomycete effectors such as chloroplasts and processing bodies. More than half of the 30 core effectors were able to suppress pattern-triggered immunity in N. benthamiana, and five of these induced hypersensitive responses (HR) in sunflower broad-spectrum resistant lines. HR triggered by PhRXLRC01 co-segregated with Pl22 resistance in F3 populations and both traits localized in 1.7 Mb on chromosome 13 of the sunflower genome. Pl22 resistance was physically mapped on the sunflower genome recently sequenced, unlike all the other downy mildew resistances published so far. PhRXLRC01 and Pl22 are proposed as an avirulence/resistance gene couple not previously described in sunflower. Core effector recognition is a successful strategy to accelerate broad-spectrum resistance gene identification in complex crop genomes such as sunflower., (© 2018 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.)

Published
2019
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18. Brachypodium distachyon tar2l hypo mutant shows reduced root developmental response to symbiotic signal but increased arbuscular mycorrhiza.

Author

Buendia L, Ribeyre C, Bensmihen S, and Lefebvre B

Subjects
Brachypodium drug effects, Chitin analogs & derivatives, Chitin pharmacology, Chitosan, Indoleacetic Acids metabolism, Indoleacetic Acids pharmacology, Indoles pharmacology, Mycorrhizae drug effects, Mycorrhizae growth & development, Oligosaccharides, Plant Roots drug effects, Symbiosis drug effects, Brachypodium genetics, Brachypodium microbiology, Mutation genetics, Mycorrhizae physiology, Plant Proteins genetics, Plant Roots growth & development, Symbiosis physiology
Abstract

Auxin is a major phytohormone that controls root development. A role for auxin is also emerging in the control of plant-microbe interactions, including for the establishment of root endosymbiosis between plants and arbuscular mycorrhizal fungi (AMF). Auxin perception is important both for root colonization by AMF and for arbuscule formation. AMF produce symbiotic signals called lipo-chitooligosaccharides (LCOs) that can modify auxin homeostasis and promote lateral root formation (LRF). Since Brachypodium distachyon (Brachypodium) has a different auxin sensitivity compared to other plant species, we wondered whether this would interfere with the effect of auxin in arbuscular mycorrhizal (AM) symbiosis. Here we tested whether tar2l hypo a Brachypodium mutant with an increase in endogenous auxin content is affected in LRF stimulation by LCOs and in AM symbiosis. We found that, in contrast to control plants, LCO treatment inhibited LRF of the tar2l hypo mutant. However, the level of AMF colonization and the abundance of arbuscules were increased in tar2l hypo compared to control plants, suggesting that auxin also plays a positive role in both AMF colonization and arbuscule formation in Brachypodium.

Published
2019
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19. LysM Receptor-Like Kinase and LysM Receptor-Like Protein Families: An Update on Phylogeny and Functional Characterization.

Author

Buendia L, Girardin A, Wang T, Cottret L, and Lefebvre B

Abstract

Members of plant specific families of receptor-like kinases (RLKs) and receptor-like proteins (RLPs), containing 3 extracellular LysMs have been shown to directly bind and/or to be involved in perception of lipo-chitooligosaccharides (LCO), chitooligosaccharides (CO), and peptidoglycan (PGN), three types of GlcNAc-containing molecules produced by microorganisms. These receptors are involved in microorganism perception by plants and can activate different plant responses leading either to symbiosis establishment or to defense responses against pathogens. LysM-RLK/Ps belong to multigenic families. Here, we provide a phylogeny of these families in eight plant species, including dicotyledons and monocotyledons, and we discuss known or putative biological roles of the members in each of the identified phylogenetic groups. We also report and discuss known biochemical properties of the LysM-RLK/Ps.

Published
2018
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20. RXLR and CRN Effectors from the Sunflower Downy Mildew Pathogen Plasmopara halstedii Induce Hypersensitive-Like Responses in Resistant Sunflower Lines.

Author

Gascuel Q, Buendia L, Pecrix Y, Blanchet N, Muños S, Vear F, and Godiard L

Abstract

Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus , an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances.

Published
2016
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21. The LysM receptor-like kinase SlLYK10 regulates the arbuscular mycorrhizal symbiosis in tomato.

Author

Buendia L, Wang T, Girardin A, and Lefebvre B

Subjects
Gene Expression Regulation, Plant, Gene Silencing, Solanum lycopersicum genetics, Phylogeny, Plant Proteins genetics, Sequence Homology, Amino Acid, Glomeromycota physiology, Solanum lycopersicum enzymology, Solanum lycopersicum microbiology, Mycorrhizae physiology, Plant Proteins metabolism, Receptor Protein-Tyrosine Kinases metabolism, Symbiosis genetics
Abstract

Most plants have the ability to establish a symbiosis with arbuscular mycorrhizal (AM) fungi, which allows better plant nutrition. A plant signaling pathway, called the common symbiosis signaling pathway (CSSP), is essential for the establishment of both AM and root nodule symbioses. The CSSP is activated by microbial signals. Plant receptor(s) for AM fungal signals required for the activation of the CSSP and initial fungal penetration are currently unknown. We set up conditions to use virus-induced gene silencing (VIGS) in Solanum lycopersicum to study the genes potentially involved in AM. We show that the lysin motif receptor-like kinase SlLYK10, whose orthologs in legumes are essential for nodulation, but not for AM, and SlCCaMK, a component of the CSSP, are required for penetration of the AM fungus Rhizophagus irregularis into the roots of young tomato plants. Our results support the hypothesis that the SILYK10 ancestral gene originally played a role in AM and underwent duplication and neofunctionalization for a role in nodulation in legumes. Moreover, we conclude that VIGS is an efficient method for fast screening of genes playing major roles in AM., (© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.)

Published
2016
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22. Tolerance, arsenic uptake, and oxidative stress in Acacia farnesiana under arsenate-stress.

Author

Alcantara-Martinez N, Guizar S, Rivera-Cabrera F, Anicacio-Acevedo BE, Buendia-Gonzalez L, and Volke-Sepulveda T

Subjects
Acacia enzymology, Acacia genetics, Arsenates metabolism, Arsenic metabolism, Arsenic toxicity, Biodegradation, Environmental, Glutathione Transferase metabolism, Lipid Peroxidation drug effects, Oxidative Stress, Peroxidases metabolism, Plant Proteins metabolism, Seedlings drug effects, Seedlings enzymology, Seedlings growth & development, Seedlings metabolism, Soil Pollutants metabolism, Acacia drug effects, Acacia metabolism, Arsenates toxicity, Soil Pollutants toxicity
Abstract

Acacia farnesiana is a shrub widely distributed in soils heavily polluted with arsenic in Mexico. However, the mechanisms by which this species tolerates the phytotoxic effects of arsenic are unknown. This study aimed to investigate the tolerance and bioaccumulation of As by A. farnesiana seedlings exposed to high doses of arsenate (AsV) and the role of peroxidases (POX) and glutathione S-transferases (GST) in alleviating As-stress. For that, long-period tests were performed in vitro under different AsV treatments. A. farnesiana showed a remarkable tolerance to AsV, achieving a half-inhibitory concentration (IC50) of about 2.8 mM. Bioaccumulation reached about 940 and 4380 mg As·kg(-1) of dry weight in shoots and roots, respectively, exposed for 60 days to 0.58 mM AsV. Seedlings exposed to such conditions registered a growth delay during the first 15 days, when the fastest As uptake rate (117 mg kg(-1) day(-1)) occurred, coinciding with both the highest rate of lipid peroxidation and the strongest up-regulation of enzyme activities. GST activity showed a strong correlation with the As bioaccumulated, suggesting its role in imparting AsV tolerance. This study demonstrated that besides tolerance to AsV, A. farnesiana bioaccumulates considerable amounts of As, suggesting that it may be useful for phytostabilization purposes.

Published
2016
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23. Reducing greenhouse gas emissions and adapting agricultural management for climate change in developing countries: providing the basis for action.

Author

Ogle SM, Olander L, Wollenberg L, Rosenstock T, Tubiello F, Paustian K, Buendia L, Nihart A, and Smith P

Subjects
Air Pollutants analysis, Air Pollution analysis, Developing Countries, Agriculture methods, Air Pollution prevention & control, Climate Change
Abstract

Agriculture in developing countries has attracted increasing attention in international negotiations within the United Nations Framework Convention on Climate Change for both adaptation to climate change and greenhouse gas mitigation. However, there is limited understanding about potential complementarity between management practices that promote adaptation and mitigation, and limited basis to account for greenhouse gas emission reductions in this sector. The good news is that the global research community could provide the support needed to address these issues through further research linking adaptation and mitigation. In addition, a small shift in strategy by the Intergovernmental Panel on Climate Change (IPCC) and ongoing assistance from agricultural organizations could produce a framework to move the research and development from concept to reality. In turn, significant progress is possible in the near term providing the basis for UNFCCC negotiations to move beyond discussion to action for the agricultural sector in developing countries., (© 2013 John Wiley & Sons Ltd.)

Published
2014
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24. Effect of a saprophytic fungus on the growth and the lead uptake, translocation and immobilization in Dodonaea viscosa.

Author

Rojas-Loria CC, Peralta-Perez Mdel R, Buendia-Gonzalez L, and Volke-Sepulveda TL

Subjects
Biodegradation, Environmental, Biological Transport, Biomass, Lead analysis, Plant Roots growth & development, Plant Roots metabolism, Plant Roots microbiology, Sapindaceae drug effects, Sapindaceae growth & development, Sapindaceae metabolism, Soil, Ascomycota physiology, Lead metabolism, Sapindaceae microbiology, Soil Pollutants metabolism
Abstract

Phytoremediation is a feasible alternative to remediate soils polluted with toxic elements, which can be enhanced by manipulating plant-microbe interactions. Regarding this, free-living saprophytic fungi that interact beneficially with roots have been scarcely studied. Thus, the aim of this study was to assess the effect of a saprophytic fungus, Lewia sp., on the plant growth and the ability of Dodonaea viscosa to phytoaccumulate or phytostabilize soluble and insoluble sources of lead in a solid support. The growth of D. viscosa was influenced by both Pb and Lewia sp. While seedlings exposed to Pb showed a decrease in biomass production, in seedlings grown without Pb the biomass was stimulated by Lewia sp. The fungus strongly stimulated the weight-to-length ratio in roots. Regardless of the treatment, D. viscosa accumulated 4.4-6.5 times more Pb in roots than in shoots, conducting to low translocation factors (< 0.2). The presence of Lewia sp. significantly improved Pb accumulation, achieving high bioconcentration factors (> 22), which was attributed to an increased bioavailability and uptake of Pb due to the fungus. This study demonstrated that Lewia sp. could improve Pb-phytostabilization by D. viscosa in soils polluted with soluble and insoluble forms of Pb.

Published
2012
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