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Inter-alpha-trypsin inhibitor (ITI) is a 180-kDa glycoprotein having the role of a serine protease inhibitor. It has been revealed that ITI is not a single polypeptide chain structure, but a complex of three kinds of subunits, two heavy (H1 and H2) chains and a light (L) chain (Bourguignon et al. 1983) with chondroitin sulfate cross-links (Jessen et al. 1988). In situ hybridization showed that the H1, H2 and L chains were encoded by separate genes located on chromosomes 3, 10 and 9, respectively (Diarra-Mehrpour et al. 1989). Genetic variation of ITI on an isoelectric point was reported by Vogt and Cleve (1990) being controlled by mainly three alleles (ITI*1, ITI*2 and ITI*3) with autosomal codominant inheritance. The utility of ITI system has been supported from the results of several population studies (Luckenbach et al. 1991; Vogt et al. 1991a, 1991b; Yuasa et al. 1991; Vogt et al. 1992). However, Vogt et al. (1991b) indicated the cautious application of ITI for paternity testing due to an incomplete expression of ITI phenotypes among infants.

KUMAGAI, R., TOKIWA, K., NAKAYASHIKI, N. and KATSURA, S. Glutamate- Pyruvate Transaminase (GPT) Variant Found in a Disputed Paternity Case. Tohoku J. exp. Med., 1987, 153 (4), 383-388 - GPT variants, GPT2C-1 and GPT2A-2C, were found in samples from a mother and her child, respectively, in a disputed paternity case. The phenotype GPT 2C-1 was determined by polyacrylamide gel electrophoresis performed at alkaline pH and acidic pH; this is probably the first GPT2C-1 detected in Japanese. It was considered that the GPT*2C allele in this family was inherited in a manner of autosomal codominant transmission.