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Introduction: Streptococcus suis serotype ( cps ) 2 is an important bacterial pathogen in piglet nurseries and an emerging zoonotic agent without effective vaccines available. Immunoglobulin (Ig)M plays an essential role in host defense against S. suis . In mice, non-conventional B-1 cells are a major source of protective IgM against encapsulated bacterial pathogens, such as S. pneumoniae . Two IgM + CD21 - B-1-like cell subpopulations, distinguishable by CD11R1 expression, were described in pigs, but their properties and functions are poorly understood. This study aimed at a first characterization of the porcine early IgM B cell response against S. suis cps 2., Methods: We analyzed the same healthy pigs, naturally colonized by different S. suis serotypes, including cps 2, at four and eight weeks of age serologically and determined the frequency of different peripheral B cell subpopulations by flow cytometry. Furthermore, we isolated conventional IgM + CD21 + B-2 cells as well as non-conventional B-1-like cell subpopulations from peripheral blood of eight-weeks-old pigs to evaluate their potential of IgM secretion in response to innate and adaptive stimuli in vitro ., Results: Between the fourth and eighth week of life, a characteristic increase of S. suis cps 2-binding serum IgM antibodies, restricting bacterial growth, was observed. Moreover, we show for the first time that the significant increase of anti- S. suis serum IgM is associated with a relative increase of peripheral non-conventional IgM + CD21 - B-1-like cells in vivo , particularly of the IgM + CD21 - CD11R1 - subpopulation. Noteworthy, sorted IgM + CD21 - CD11R1 - B-1-like cells from eight-weeks-old pigs spontaneously secreted IgM in vitro . In addition, both non-conventional IgM + CD21 - B cell subpopulations, in contrast to conventional IgM + CD21 + B-2 cells, produced anti- S. suis IgM upon toll-like receptor (TLR) stimulation underlining their innate-like characteristics. We furthermore observed that both B-1-like subpopulations secrete S. suis cps 2-binding IgM upon stimulation with T cell-associated factors with highest amounts in IgM + CD21 - CD11R1 - B-1-like cells even exceeding anti- S. suis IgM levels produced by B-2 cells., Conclusion: Porcine non-conventional B-1-like cells are a potent source of S. suis -binding IgM indicating a role in immunity during a critical phase of piglet rearing., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Seidel, Kauffold, Protschka, Baums, Alber and Eschke.)

Background: Scrotal swelling is a clinical situation which can be caused by different aetiologies. In this case report, we describe a multi-week episode of unilateral and bilateral scrotal swelling in boars at an Austrian boar stud and its diagnostic work-up., Case Presentation: In the summer of 2020, the herd veterinarian of an Austrian boar stud reported that over a period of six weeks, five out of 70 boars presented with unilateral severe swelling of the left scrotum and three out of 70 boars with bilateral severe swelling of the left and moderate swelling of the right scrotum, respectively. A complete history was obtained and an on-site evaluation of the facility was done. Five boars were necropsied, and a variety of samples harvested for further diagnostic investigations. Infectious differential diagnoses associated with unilateral swelling of the scrotum or the testis were excluded through serological and tissue testing. In three of the five boars, histopathology revealed complete acute haemorrhagic necrosis of the left testis concurrent with strongly congested blood vessels. Review of the collected information with a group of experts in the field of boar stud management resulted with consensus that, most likely, trauma was the etiologic event causing the clinical signs and pathology. Coincident with discussion of implementing video recording cameras in the boar housing area, no further clinical cases followed. As this case occurred during the first lockdown of the COVID-19 pandemic, we propose that the distress and travelling restrictions may have contributed to frustration among boar stud workers, which was consequently expressed as misbehaviour against boars., Conclusions: Once all known infectious causes of unilateral swelling of the scrotum were excluded, a critical diagnostic work-up focused on non-infectious causes. Non-infectious causes, such as trauma, need to be carefully evaluated, as it may also include human misbehaviour against boars. Summarizing all findings of this case report, the authors hypothesize that a blunt trauma was the reason for the series of mainly unilateral swelling of the scrota of boars., (© 2023. The Author(s).)

The modern hyperprolific sow is susceptible to metabolic disease and chronic inflammation. The most sensitive phase is parturition, when the sow experiences systemic inflammation and stress, and major changes in metabolism and endocrinology. Resolution of inflammation and stress needs to happen quickly to ensure good reproductive health during puerperium. If the sow fails to adapt to these changes, puerperal disease may occur. The economically most important puerperal disease complex is the postpartum dysgalactia syndrome (PPDS). Other puerperal diseases include infections of the urogenital tract. Diagnosis of PPDS and urogenital disease on-farm is challenging but several diagnostic methods, including clinical examination, behavioral observations, ultrasonography and biomarkers are available. Ultrasonography is an excellent tool for monitoring the health of the urogenital tract, the mammary gland, and uterine involution and guide further diagnostic interventions. Biomarkers such as Chromogranin A, tumor necrosis factor-α, and interleukin-6 represent promising tools to monitor general health and the systemic state of inflammation and oxidative stress of the sow. Nonsteroidal anti-inflammatory drugs, dopamine antagonists, and oxytocin are promising to address the symptoms of PPDS. Reducing of stress, improving nutrition and intestinal health, and supporting animal welfare-friendly husbandry help in the prevention of PPDS., (© 2022 The Authors. Molecular Reproduction and Development published by Wiley Periodicals LLC.)

Background: Feedgrain contamination with mycotoxins, including deoxynivalenol (DON, "vomitoxin") is relatively frequently encountered. Pigs are particularly sensitive to the toxicity of DON. To assess the interplay between DON and porcine reproductive and respiratory syndrome virus (PRRSV), we performed an experimental DON exposure-PRRSV vaccination-challenge infection trial. Three-week-old piglets were divided into four groups. Groups I, II and III (10 animals/group) were vaccinated with a PRRSV modified live vaccine and 2 weeks later challenged with a heterologous field strain. While group I was not supplemented with DON, animals in groups II and III received DON for 4 weeks prior to challenge infection at levels that can be encountered in pig feed, employing a low-dose or high-dose regime (group II: 40 µg DON/kg body weight per day; group III: 80 µg DON/kg body weight per day, corresponding to approx. 1 or 2 mg DON/kg feed, respectively). Eight animals (group IV; unvaccinated, not DON exposed) served as control animals for the challenge infection., Results: We assessed clinical signs, virus load in serum and various organs as well as antibody titres in the animals. All vaccinated animals mounted an efficient PRRSV-specific antibody response within 2 weeks, except for 20% of the animals receiving the higher DON dose. Upon virus challenge, the vaccinated animals in group I were protected from clinical signs. Vaccinated DON-exposed animals in group II and III were protected from clinical signs to a lesser extent. Clinical signs in group III receiving the higher dose of DON were as severe as in the (unvaccinated, not DON exposed) control group IV. The animals of group III also displayed lower antibody titres compared with the animals in group I and II., Conclusions: The experimental vaccination/challenge study therefore revealed that exposure of pigs to DON for a period of 4 weeks deteriorates the efficacy of vaccination against clinical signs of PRRS., (© 2022. The Author(s).)

Objective: The aim of this study was to analyse the continuous and subjective evaluation of echogenicity, echotexture of the puerperal uterus as well as the presence of intrauterine fluid in sows of differing parity stages. The goal was to determine associations between grayscale values and simultaneously assessed uterine diameter, body condition, and additional performance parameters such as farrowing duration, placenta expulsion duration, placenta weight and litter weight., Material and Methods: The study population included 95 sows housed either in free farrowing (n = 46) or crated (n = 49) stables. Transabdominal ultrasound was performed daily between postpartum days 2-14 (p. p.) and the day before weaning. Echogenicity (ranging between anechoic (1)-hyperechoic (6)), echotexture (homogenous/heterogeneous), and the presence of intrauterine fluid (yes/no) was evaluated on three cross-sections of the uterine horn/sow/day., Results: In the first week p.p, most sows showed a medium (median = 4.0) echogenicity, whereas from day 7 p. p., echogenicity decreased (median = 3.5 to 3.0). Echotexture between days 2-7 p.p was mainly heterogeneous, whereas between days 8-14 p.p, it proved to be mostly homogeneous. Intrauterine fluid was detected until day 9. Echogenicity exhibited significant association with farrowing duration (FD) on days 8-14 p. p. (p = 0.048). Sows with FD > 300 min showed slightly lower echogenicity (median = 3.0) than sows with FD ≤ 300 min (median = 3.5). There was a positive correlation between uterine diameter and echogenicity on day 2-7 p. p. (r = 0,359, p < 0.001) and days 8-14 p. p. (r = 0,381, p < 0.001)., Conclusions: Subjective grayscale analysis by ultrasonography is suitable in characterizing uterine echogenicity and echotexture as well as intrauterine fluid in post-partum sows. The presented findings suggest that this may aide in assessing patterns of the sow's puerperium., Clinical Relevance: The subjective grayscale analysis is suitable in clinically assessing echogenicity and echotexture during the postpartum phase. The subjective grayscale analysis provides initial indications concerning status of uterine health and involution in sows. Based on the ultrasonographic findings, treatment may be initiated and subsequently evaluated. Furthermore, the results of the subjective grey analysis in combination with further performance characteristics can be implemented to cull breeding sows. Furthermore, the results of the subjective grey analysis in combination with additional performance characteristics may be used in order to cull/reject sows exhibiting pathophysiologic findings from further breeding., Competing Interests: Die Autoren bestätigen, dass kein Interessenkonflikt besteht., (Thieme. All rights reserved.)

Objective: Deoxynivalenol (DON) is a mycotoxin produced by fungi of Fusarium spp. It causes vomiting, but also exerts immunosuppressive effects that may be seen at local level e. g. the urogenital tract, in which case these effects are of relevance for fertility. The presented report describes a DON-associated pathomorphological picture of the urogenital tract in conjunction with the associated microbiological colonization., Material and Methods: A total of 19 genital tracts (vagina, cervix, uterus, oviducts and ovaries) including the urinary bladder (n = 15) from reproductively failed gilts and different parity sows submitted from 8 farms in 2019/20 were examined pathomorphologically. DON as well as zearalenone (ZEA) were determined by using high performance liquid chromatography in 11 individual and 2 pooled (2 and 4 animals, respectively) bile samples. Microbiologic examinations of uterine (n = 17) and bladder (n = 12) specimens were additionally performed., Results: Nearly all vaginas (n = 16), cervixes (n = 15), uteri (n = 18) and numerous oviducts (n = 11) as well as urinary bladders (n = 11) displayed a mostly medium to severe chronical inflammation. In 17 cases, ≥ 2 organs were affected. Microbiologic evaluation revealed a usually mixed flora of several gram-negative and gram-positive bacterial species with E. coli, Streptococcus spp., Aeromonas spp. and Enterococcus spp. being the predominant species. A total of 8 individual and both pooled bile samples were found DON-positive (75.5 to > 200.0 µg/l). In a subset of the DON-positive samples, ZEA was simultaneously detected at low concentrations (6.57-21.20 µg/l)., Conclusion and Clinical Relevance: The results of the present study allow for the postulation that DON may cause fertility problems. Subsequent pathomorphological examinations of genital organs and the urinary bladder are recommended. When ≥ 2 organs are chronically inflamed and the uteri are additionally microbiologically positive, a contribution of DON may be assumed and confirmation via bile analysis is warranted., Competing Interests: Die Autoren bestätigen, dass kein Interessenkonflikt besteht., (Thieme. All rights reserved.)

Litter size in modern so called hyperprolific pig (Sus scrofa Linnaeus) breeds such as of crossbred Danish Landrace x Danish Yorkshire (LY/YL) sows increased remarkably over recent years, however, commonly associated with reduced piglet birth weight and higher within litter birth weight variability likely due to a limited uterine capacity. Since investigation into this issue is patchy, the aim of this study was to investigate uterine capacity based on litter and placental characteristics in two sow lines with different prolificacy, that is crossbred Danish genetic (Danish Landrace x Danish Yorkshire; DG; n = 14) and purebred German Saddleback (GS) sows (n = 12). Parameters recorded were litter size, piglet birth weight and vitality, placental weight and surface area as well as placental vascularization. Litters of DG were on average larger than of GS (p < .001). Piglets of DG weighed on average less than GS (p < .001) and were less vital (p < .001-.142). Increasing litter size was associated with reduced piglet birth weight and increased within litter birth weight variability in GS, but not in DG. DG had on average a lower placental weight (p < .001) and smaller placentae (p < .001) than GS, but the placenta was on average more efficient than of GS (based on the quotient of piglet and corresponding placental weight; p < .001). Vascularization of placentae was on average not or only slightly different between breeds (p < .05 - .982). Remarkably, however, vascularization of the lateral and apical chorionic epithelium of the chorionic ridges as the immediate foetal/maternal interface was on average slightly higher in DG than GS (p < .05-.111). Results thus demonstrate that uterine capacity based on litter and placental characteristics is higher in DG than GS sows., (© 2020 The Authors. Reproduction in Domestic Animals published by Wiley-VCH GmbH.)

Within the past 30 years, through ongoing technology and portability developments, real-time (b-mode) ultrasonography (RTU) has increasingly become a valuable diagnostic tool in assessing the female reproductive tract in swine. Initially applied in swine production to visually determine pregnancy status, RTU use has expanded to include assessment of the peri-pubertal and mature non-pregnant females as well. Transabdominal and transrectal modalities to visualizing the reproductive tract in swine have been reported with the transabdominal approach more common due to the fact of its ease of accessibility, animal/personnel safety, and reduced time to perform. Adjustable frequency transducers are preferred as they allow optimization of image quality at various depths. If a single transducer frequency must be selected, a 5 MHz probe provides the best versatility for visualizing the reproductive tract in swine. Other basic requirements for ultrasound equipment which will be used on commercial swine farms include being light weight and easy to handle, readily cleanable and disinfectable, long battery-life, and good durability. When using RTU for pregnancy determination, diagnosis is based upon a combination of the animal's breeding records, the presence of embryonic fluid, and, depending upon gestational stage, fetal structures. If RTU is used as a diagnostic tool in assessing reproductive problems in an individual or a group of animals, sonographic evaluation of both the uterus and ovaries is performed. Tissues are delineated and assessed based upon their echogenicity, echotexture, and size. Uses of RTU in clinical practice may include assessment of delayed puberty, prolonged wean-to-estrus interval, absence of post-weaning estrus, herd disruptions in conception and farrowing rates, vulval discharge, peripartum and puerperal disorders. This review aims to provide an overview on principles and clinical uses of RTU with respect to application to address female reproductive performance issues in commercial swine operations.