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Your search keyword '"Huang, Wei-Hua"' showing total 840 results
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840 results on '"Huang, Wei-Hua"'

7. Nanoelectrodes for intracellular measurements of reactive oxygen and nitrogen species in single living cells

Author

Hu, Keke, Liu, Yan-Ling, Oleinick, Alexander, Mirkin, Michael, Huang, Wei-Hua, and Amatore, Christian

Subjects
Quantitative Biology - Subcellular Processes, Physics - Biological Physics
Abstract

Reactive oxygen and nitrogen species (ROS and RNS) play important roles in various physiological processes (e.g., phagocytosis) and pathological conditions (e.g., cancer). The primary ROS/RNS, viz., hydrogen peroxide, peroxynitrite ion, nitric oxide, and nitrite ion, can be oxidized at different electrode potentials and therefore detected and quantified by electroanalytical techniques. Nanometer-sized electrochemical probes are especially suitable for measuring ROS/RNS in single cells and cellular organelles. In this article, we survey recent advances in localized measurements of ROS/RNS inside single cells and discuss several methodological issues, including optimization of nanoelectrode geometry, precise positioning of an electrochemical probe inside a cell, and interpretation of electroanalytical data.

Published
2020
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21. In vivo pharmacokinetics of ginsenoside compound K mediated by gut microbiota.

Author

Deng, Ming-Si, Huang, Su-tian-zi, Xu, Ya-Ni, Shao, Li, Wang, Zheng-Guang, Chen, Liang-Jian, and Huang, Wei-Hua

Subjects
LIQUID chromatography-mass spectrometry, GUT microbiome, GINSENOSIDES, PHARMACOKINETICS, RATS, BIOCONVERSION
Abstract

Ginsenoside Compound K (GCK) is the main metabolite of natural protopanaxadiol ginsenosides with diverse pharmacological effects. Gut microbiota contributes to the biotransformation of GCK, while the effect of gut microbiota on the pharmacokinetics of GCK in vivo remains unclear. To illustrate the role of gut microbiota in GCK metabolism in vivo, a systematic investigation of the pharmacokinetics of GCK in specific pathogen free (SPF) and pseudo-germ-free (pseudo-GF) rats were conducted. Pseudo-GF rats were treated with non-absorbable antibiotics. Liquid chromatography tandem mass spectrometry (LC–MS/MS) was validated for the quantification of GCK in rat plasma. Compared with SPF rats, the plasma concentration of GCK significantly increased after the gut microbiota depleted. The results showed that GCK absorption slowed down, Tmax delayed by 3.5 h, AUC0-11 increased by 1.3 times, CLz/F decreased by 0.6 times in pseudo-GF rats, and Cmax was 1.6 times higher than that of normal rats. The data indicated that gut microbiota played an important role in the pharmacokinetics of GCK in vivo. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Electroactive polymer tag modified nanosensors for enhanced intracellular ATP detection.

Author

Kang, Yi-Ran, Jiao, Yu-Ting, Zhao, Chen-Fei, Zhang, Xin-Wei, and Huang, Wei-Hua

Subjects
CONDUCTING polymers, NANOSENSORS, POWER resources, POLYMERS, SIGNAL detection, ADENOSINE triphosphate, MONOMERS, APTAMERS
Abstract

ATP plays a crucial role in cell energy supply, so the quantification of intracellular ATP levels is particularly important for understanding many physio-pathological processes. The intracellular quantification of this non-electroactive molecule can be realized using aptamer-modified nanoelectrodes, but is hindered by the limited quantity of modification and electroactive tags on the nanosized electrodes. Herein, we developed a simple but effective electrochemical signal amplification strategy for intracellular ATP detection, which replaces the regular ATP aptamer-linked ferrocene monomer with a polymer, thus greatly magnifying the amounts of electrochemical reporters linked to one chain of the aptamer and enhancing the signals. This ferrocene polymer–ATP aptamer was further immobilized onto Au nanowire electrodes (SiC@C@Au NWEs) to achieve accurate quantification of intracellular ATP in single cells, presenting high electrochemical signal output and high specificity. This work not only provides a powerful tool for quantifying intracellular ATP but also offers a simple and versatile strategy for electrochemical signal amplification in the detection of broader non-electroactive molecules involved in different kinds of intracellular physiological processes. [ABSTRACT FROM AUTHOR]

Published
2024
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25. Nanoelectrochemistry monitoring of intracellular reactive oxygen and nitrogen species induced by nanoplastic exposure.

Author

Wu, Hui-Qian, Qi, Yu-Ting, Guo, Bing-Yi, Zhao, Yi, Zhang, Xin-Wei, and Huang, Wei-Hua

Subjects
REACTIVE nitrogen species, REACTIVE oxygen species, OXIDATIVE stress, NURSES, LUNG injuries
Abstract

Airborne nanoplastics can enter alveolar cells and trigger intracellular oxidative stress primarily. Herein, taking advantage of the high electrochemical resolution of SiC@Pt nanoelectrodes, we achieved the quantitative discrimination of the major ROS/RNS within A549 cells, disclosed the sources of their precursors, and observed that the NO (RNS precursor) level significantly increased, whereas O2˙− (ROS precursor) remained relatively stable during the nanoplastics exposure. This establishes that iNOS or mitochondrion-targeted treatment may be a preventive or therapeutic strategy for nanoplastic-induced lung injury. [ABSTRACT FROM AUTHOR]

Published
2024
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