Purpose: The established automatic AI tooth segmentation algorithm was used to achieve rapid and automatic tooth segmentation from CBCT images. The three-dimensional data obtained by oral scanning of real isolated teeth were used as the gold standard to verify the accuracy of the algorithm., Methods: Thirty sets of CBCT data and corresponding 59 isolated teeth were collected from Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. The three-dimensional tooth data in CBCT images were segmented by the algorithm. The digital information obtained by scanning the extracted teeth after processing was used as the gold standard. In order to compare the difference between the segmentation results and the scanning results of the algorithm. The Dice coefficient(Dice), sensitivity (Sen) and average symmetric surface distance (ASSD) were selected to evaluate the segmentation accuracy of the algorithm. The intra-class correlation coefficient(ICC) was used to evaluate the differences in length, area, and volume between the single tooth obtained by the AI system and the digital isolated tooth. Due to the existence of CBCT with different resolution, ANOVA was used to analyze the differences between groups with different resolution, and SNK method was used to compare them between two groups. SPSS 25.0 software package was used to analyze the data., Results: After comparing the segmentation results with the in vitro dental scanning results, the average Dice value was (94.7±1.88)%, the average Sen was (95.8±2.02)%, and the average ASSD was (0.49±0.12) mm. By comparing the length, area and volume of a single tooth obtained by the digital isolated tooth and the AI system, the ICC values of the intra-group correlation coefficients were 0.734, 0.719 and 0.885, respectively. The single tooth divided by the AI system has a good consistency with the digital model in evaluating the length, area and volume, but the segmentation results were still different from the real situation in terms of specific values. The smaller the voxel of CBCT, the higher the resolution, the better the segmentation results., Conclusions: The CBCT tooth segmentation algorithm established in this study can accurately achieve the tooth segmentation of the whole dentition in CBCT at all resolutions. The improvement of CBCT resolution ratio can make the algorithm more accurate. Compared with the current segmentation algorithms, our algorithm has better performance. Compared with the real situation, there are still some differences, and the algorithm needs to be further improved and verified.